Phospho-CtBP1 (Ser422) Antibody | Affinity Biosciences

WB
IHC
pElisa

1/8

Western blot analysis of extracts from Mouse heart, using Phospho-CtBP1 (Ser422) Antibody.

AF0807 at 1/100 staining human kidney tissue sections by IHC-P.

AF0807 at 1/100 staining Mouse brain tissue by IHC-P.

AF0807 at 1/100 staining Mouse testis tissue by IHC-P.

AF0807 at 1/100 staining Rat heart tissue by IHC-P.

AF0807 at 1/100 staining Rat liver tissue by IHC-P.

AF0807 at 1/100 staining Rat brain tissue by IHC-P.

peptide-ELISA analysis of AF0807.

Product:	Phospho-CtBP1 (Ser422) Antibody
Catalog:	AF0807
Description:	Rabbit polyclonal antibody to Phospho-CtBP1 (Ser422)
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	48kDa; 48kD(Calculated).
Uniprot:	Q13363
RRID:	AB_2834100

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Phospho-CtBP1 (Ser422) Antibody detects endogenous levels of CtBP1 only when phosphorylated at Sersine 422.

RRID:

AB_2834100
Cite Format: Affinity Biosciences Cat# AF0807, RRID:AB_2834100.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BARS; brefeldin A- ribosylated substrate; C terminal binding protein 1; C-terminal-binding protein 1; CTBP; CtBP1; CTBP1_HUMAN; MGC104684;

Immunogens

Immunogen:

Uniprot:

Q13363(CTBP1_HUMAN) >>Visit HPA database.

Gene(ID):

CTBP1(1487)

Expression:

Q13363 CTBP1_HUMAN:

Expressed in germinal center B-cells.

Description:

This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants.

Sequence:

Q13363 CTBP1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGSSHLLNKGLPLGVRPPIMNGPLHPRPLVALLDGRDCTVEMPILKDVATVAFCDAQSTQEIHEKVLNEAVGALMYHTITLTREDLEKFKALRIIVRIGSGFDNIDIKSAGDLGIAVCNVPAASVEETADSTLCHILNLYRRATWLHQALREGTRVQSVEQIREVASGAARIRGETLGIIGLGRVGQAVALRAKAFGFNVLFYDPYLSDGVERALGLQRVSTLQDLLFHSDCVTLHCGLNEHNHHLINDFTVKQMRQGAFLVNTARGGLVDEKALAQALKEGRIRGAALDVHESEPFSFSQGPLKDAPNLICTPHAAWYSEQASIEMREEAAREIRRAITGRIPDSLKNCVNKDHLTAATHWASMDPAVVHPELNGAAYRYPPGVVGVAPTGIPAAVEGIVPSAMSLSHGLPPVAHPPHAPSPGQTVKPEADRDHASDQL

PTMs - Q13363 As Substrate

Q13363

Site	PTM Type	Enzyme	Source
K46	Ubiquitination		Uniprot
C54	S-Nitrosylation		Uniprot
Y76	Phosphorylation		Uniprot
K88	Ubiquitination		Uniprot
S100	Phosphorylation		Uniprot
T144	Phosphorylation		Uniprot
S158	Phosphorylation	Q13153 (PAK1) , Q13131 (PRKAA1)	Uniprot
K194	Ubiquitination		Uniprot
R266	Methylation		Uniprot
K273	Ubiquitination		Uniprot
K280	Ubiquitination		Uniprot
R285	Methylation		Uniprot
S300	Phosphorylation		Uniprot
K348	Ubiquitination		Uniprot
C350	S-Nitrosylation		Uniprot
S422	Phosphorylation	P45983 (MAPK8) , Q9H2X6 (HIPK2)	Uniprot
K428	Sumoylation		Uniprot

Research Backgrounds

Q13363_CTBP1_HUMAN

Function:

Corepressor targeting diverse transcription regulators such as GLIS2 or BCL6. Has dehydrogenase activity. Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation.

PTMs:

The level of phosphorylation appears to be regulated during the cell cycle. Phosphorylation by HIPK2 on Ser-422 induces proteasomal degradation.

ADP-ribosylated; when cells are exposed to brefeldin A.

Sumoylation on Lys-428 is promoted by the E3 SUMO-protein ligase CBX4.

Subcellular Location:

Cytoplasm. Nucleus.

Tissue Specificity:

Expressed in germinal center B-cells.

Subunit Structure:

Homo- or heterodimer. Heterodimer with CTBP2. Interacts with PRDM16; the interaction represses white adipose tissue (WAT)-specific genes expression. Interacts with GLIS2, FOXP2, HDAC4, HDAC5, HDAC9 and ZNF217. Interacts with ELK3 (via its PXDLS motif). Interacts with RBBP8 (via its PXDLS motif); the interaction is disrupted by binding to adenovirus E1A. Interacts with FOXP1, HIPK2, PNN, NRIP1, MECOM, ZFHX1B and WIZ. Interacts with ZNF366 (via PXDLS motif). Interaction with SATB1 (non-acetylated form); the interaction stabilizes its attachment to DNA and promotes transcription repression. Interacts with BCL6; the interaction is required for BCL6 transcriptional autoinhibition and inhibition of some BCL6 target genes. Interacts with IKZF4 (By similarity). Interacts with MCRIP1 (unphosphorylated form, via the PXDLS motif); competitively inhibiting CTBP-ZEB1 interaction. Interacts with Bassoon/BSN; this interaction targets and anchors CTBP1 to presynapses (By similarity).

(Microbial infection) Interacts with Epstein-Barr virus EBNA3 and EBNA6.

(Microbial infection) Interacts with adenovirus E1A protein (via its C-terminus); the interaction disrupts the interaction of CTBP1 with RBBP8.

(Microbial infection) Interacts with human adenovirus 5 E1A protein; this interaction seems to potentiate viral replication.

Family&Domains:

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Research Fields

· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Notch signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia. (View pathway)

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Phospho-CtBP1 (Ser422) Antibody - #AF0807