Product: Phospho-FER (Tyr402) Antibody
Catalog: AF0044
Source: Rabbit
Application: WB, IHC, IF/ICC, ELISA(peptide)
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 85kD; 95kD(Calculated).
Uniprot: P16591
RRID: AB_2834091

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-FER (Tyr402) Antibody detects endogenous levels of FER only when phosphorylated at Tyrosine 402.
RRID:
AB_2834091
Cite Format: Affinity Biosciences Cat# AF0044, RRID:AB_2834091.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

c FER; Feline encephalitis virus related kinase FER; Fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94); Fer (fps/fes related) tyrosine kinase; FER; FER_HUMAN; FerT; Fujinami poultry sarcoma/Feline sarcoma related protein Fer; p94 FER; p94-FER; Pe1Fe10; Pe1Fe13; Pe1Fe3; Pe1Fe6; Phosphoprotein NCP94; PPP1R74; Protein phosphatase 1 regulatory subunit 74; Proto oncogene tyrosine protein kinase FER; Proto-oncogene c-Fer; TYK3; Tyrosine kinase 3; Tyrosine-protein kinase Fer;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P16591 FER_HUMAN:

Isoform 1 is detected in normal colon and in fibroblasts (at protein level). Isoform 3 is detected in normal testis, in colon carcinoma-derived metastases in lung, liver and ovary, and in colon carcinoma and hepato carcinoma cell lines (at protein level). Isoform 3 is not detected in normal colon or in normal fibroblasts (at protein level). Widely expressed.

Description:
Fer protein is a member of the FPS/FES family of nontransmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors.
Sequence:
MGFGSDLKNSHEAVLKLQDWELRLLETVKKFMALRIKSDKEYASTLQNLCNQVDKESTVQMNYVSNVSKSWLLMIQQTEQLSRIMKTHAEDLNSGPLHRLTMMIKDKQQVKKSYIGVHQQIEAEMIKVTKTELEKLKCSYRQLIKEMNSAKEKYKEALAKGKETEKAKERYDKATMKLHMLHNQYVLALKGAQLHQNQYYDITLPLLLDSLQKMQEEMIKALKGIFDEYSQITSLVTEEIVNVHKEIQMSVEQIDPSTEYNNFIDVHRTTAAKEQEIEFDTSLLEENENLQANEIMWNNLTAESLQVMLKTLAEELMQTQQMLLNKEEAVLELEKRIEESSETCEKKSDIVLLLSQKQALEELKQSVQQLRCTEAKFSAQKELLEQKVQENDGKEPPPVVNYEEDARSVTSMERKERLSKFESIRHSIAGIIRSPKSALGSSALSDMISISEKPLAEQDWYHGAIPRIEAQELLKKQGDFLVRESHGKPGEYVLSVYSDGQRRHFIIQYVDNMYRFEGTGFSNIPQLIDHHYTTKQVITKKSGVVLLNPIPKDKKWILSHEDVILGELLGKGNFGEVYKGTLKDKTSVAVKTCKEDLPQELKIKFLQEAKILKQYDHPNIVKLIGVCTQRQPVYIIMELVSGGDFLTFLRRKKDELKLKQLVKFSLDAAAGMLYLESKNCIHRDLAARNCLVGENNVLKISDFGMSRQEDGGVYSSSGLKQIPIKWTAPEALNYGRYSSESDVWSFGILLWETFSLGVCPYPGMTNQQAREQVERGYRMSAPQHCPEDISKIMMKCWDYKPENRPKFSELQKELTIIKRKLT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P16591 As Substrate

Site PTM Type Enzyme
T27 Phosphorylation
K29 Ubiquitination
K40 Ubiquitination
S70 Phosphorylation
S113 Phosphorylation
Y114 Phosphorylation
K145 Ubiquitination
K155 Ubiquitination
K173 Acetylation
Y199 Phosphorylation
Y200 Phosphorylation
Y229 Phosphorylation
K326 Acetylation
K347 Acetylation
K364 Ubiquitination
K376 Ubiquitination
K381 Ubiquitination
K394 Ubiquitination
Y402 Phosphorylation
S408 Phosphorylation
T410 Phosphorylation
S411 Phosphorylation
S423 Phosphorylation
S427 Phosphorylation
S434 Phosphorylation
K453 Ubiquitination
Y461 Phosphorylation
K475 Ubiquitination
Y492 Phosphorylation
Y497 Phosphorylation
S498 Phosphorylation
K552 Ubiquitination
K579 Ubiquitination
T586 Phosphorylation
S587 Phosphorylation
K591 Ubiquitination
T592 Phosphorylation
K594 Ubiquitination
K602 Ubiquitination
K604 Ubiquitination
K610 Ubiquitination
K613 Ubiquitination
Y615 Phosphorylation P16591 (FER)
K699 Ubiquitination
Y714 Phosphorylation P16591 (FER) , P12931 (SRC)
S715 Phosphorylation
S716 Phosphorylation
K720 Ubiquitination
Y734 Phosphorylation
K795 Acetylation
K795 Ubiquitination
K812 Ubiquitination

PTMs - P16591 As Enzyme

Substrate Site Source
P10275 (AR) Y225 Uniprot
P14923 (JUP) Y550 Uniprot
P15311 (EZR) Y146 Uniprot
P15311 (EZR) Y354 Uniprot
P15311 (EZR) Y478 Uniprot
P16284 (PECAM1) Y690 Uniprot
P16284 (PECAM1) Y713 Uniprot
P16591-1 (FER) Y402 Uniprot
P16591-1 (FER) S434 Uniprot
P16591 (FER) Y615 Uniprot
P16591-1 (FER) Y714 Uniprot
P35222 (CTNNB1) Y142 Uniprot
P40763 (STAT3) Y705 Uniprot
P46459 (NSF) Y83 Uniprot
Q05397 (PTK2) Y861 Uniprot
Q05397 (PTK2) Y925 Uniprot
Q14247-3 (CTTN) Y384 Uniprot
Q14247-1 (CTTN) Y421 Uniprot

Research Backgrounds

Function:

Tyrosine-protein kinase that acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. Acts downstream of EGFR, KIT, PDGFRA and PDGFRB. Acts downstream of EGFR to promote activation of NF-kappa-B and cell proliferation. May play a role in the regulation of the mitotic cell cycle. Plays a role in the insulin receptor signaling pathway and in activation of phosphatidylinositol 3-kinase. Acts downstream of the activated FCER1 receptor and plays a role in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Plays a role in the regulation of mast cell degranulation. Plays a role in leukocyte recruitment and diapedesis in response to bacterial lipopolysaccharide (LPS). Plays a role in synapse organization, trafficking of synaptic vesicles, the generation of excitatory postsynaptic currents and neuron-neuron synaptic transmission. Plays a role in neuronal cell death after brain damage. Phosphorylates CTTN, CTNND1, PTK2/FAK1, GAB1, PECAM1 and PTPN11. May phosphorylate JUP and PTPN1. Can phosphorylate STAT3, but the biological relevance of this depends on cell type and stimulus.

PTMs:

Autophosphorylated.

Polyubiquitinated; this leads to proteasomal degradation.

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection. Cell junction. Membrane>Peripheral membrane protein>Cytoplasmic side. Nucleus. Cytoplasm>Cell cortex.
Note: Associated with the chromatin. Detected on microtubules in polarized and motile vascular endothelial cells. Colocalizes with F-actin at the cell cortex. Colocalizes with PECAM1 and CTNND1 at nascent cell-cell contacts.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1 is detected in normal colon and in fibroblasts (at protein level). Isoform 3 is detected in normal testis, in colon carcinoma-derived metastases in lung, liver and ovary, and in colon carcinoma and hepato carcinoma cell lines (at protein level). Isoform 3 is not detected in normal colon or in normal fibroblasts (at protein level). Widely expressed.

Subunit Structure:

Homotrimer. Interacts with ARHGDIA, IRS1, JAK1, NRP1, PIK3R1, PLEC and TMF1. Interacts with PPP1CA and regulates its phosphorylation at 'Thr-320' (By similarity). Interacts with CTNND1, EGFR, FLT3, PECAM1, PDGFR and STAT3. Interacts (via SH2 domain) with CTTN. Interacts with HSP90; this stabilizes phosphorylated FER and protects FER against proteasomal degradation. Component of a complex that contains at least FER, CTTN and PTK2/FAK1.

Family&Domains:

The coiled coil domains mediate homooligomerization and are required for location at microtubules.

The N-terminal region including the first coiled coil domain mediates interaction with phosphoinositide-containing membranes.

Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

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