Phospho-hnRPD (Ser83) Antibody | Affinity Biosciences

IHC
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AF0033 at 1/200 staining human brain tissue sections by IHC-P.

Product:	Phospho-hnRPD (Ser83) Antibody
Catalog:	AF0033
Description:	Rabbit polyclonal antibody to Phospho-hnRPD (Ser83)
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	38kDa; 38kD(Calculated).
Uniprot:	Q14103
RRID:	AB_2834084

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Phospho-hnRPD (Ser83) Antibody detects endogenous levels of hnRPD only when phosphorylated at Sersine 83.

RRID:

AB_2834084
Cite Format: Affinity Biosciences Cat# AF0033, RRID:AB_2834084.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ARE binding protein AUFI type A; AU-rich element RNA-binding protein 1; AUF; AUF1; AUF1A; Heterogeneous nuclear ribonucleoprotein D0; hnRNP D; hnRNP D0; Hnrnpd; hnRNPD0; HNRPD; HNRPD_HUMAN; P37;

Immunogens

Immunogen:

Uniprot:

Q14103(HNRPD_HUMAN) >>Visit HPA database.

Gene(ID):

HNRNPD(3184)

Description:

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants.

Sequence:

Q14103 HNRPD_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSEEQFGGDGAAAAATAAVGGSAGEQEGAMVAATQGAAAAAGSGAGTGGGTASGGTEGGSAESEGAKIDASKNEEDEGHSNSSPRHSEAATAQREEWKMFIGGLSWDTTKKDLKDYFSKFGEVVDCTLKLDPITGRSRGFGFVLFKESESVDKVMDQKEHKLNGKVIDPKRAKAMKTKEPVKKIFVGGLSPDTPEEKIREYFGGFGEVESIELPMDNKTNKRRGFCFITFKEEEPVKKIMEKKYHNVGLSKCEIKVAMSKEQYQQQQQWGSRGGFAGRARGRGGGPSQNWNQGYSNYWNQGYGNYGYNSQGYGGYGGYDYTGYNNYYGYGDYSNQQSGYGKVSRRGGHQNSYKPY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Pig

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14103 As Substrate

Q14103

Site	PTM Type	Enzyme	Source
S2	Acetylation		Uniprot
S2	Phosphorylation		Uniprot
S71	Phosphorylation		Uniprot
K72	Acetylation		Uniprot
K72	Ubiquitination		Uniprot
S80	Phosphorylation		Uniprot
S82	Phosphorylation		Uniprot
S83	Phosphorylation	P49841 (GSK3B)	Uniprot
S87	Phosphorylation	P17612 (PRKACA)	Uniprot
T91	Phosphorylation		Uniprot
R94	Methylation		Uniprot
S105	Phosphorylation		Uniprot
K110	Acetylation		Uniprot
K110	Ubiquitination		Uniprot
K111	Acetylation		Uniprot
K111	Ubiquitination		Uniprot
K114	Acetylation		Uniprot
K114	Ubiquitination		Uniprot
K119	Acetylation		Uniprot
K119	Ubiquitination		Uniprot
C126	S-Nitrosylation		Uniprot
T127	Phosphorylation		Uniprot
K129	Acetylation		Uniprot
K129	Ubiquitination		Uniprot
T134	Phosphorylation		Uniprot
S137	Phosphorylation		Uniprot
R138	Methylation		Uniprot
K153	Acetylation		Uniprot
K153	Ubiquitination		Uniprot
K158	Acetylation		Uniprot
K165	Acetylation		Uniprot
K165	Ubiquitination		Uniprot
K183	Ubiquitination		Uniprot
S190	Phosphorylation		Uniprot
T193	Phosphorylation	P24941 (CDK2)	Uniprot
K197	Acetylation		Uniprot
K197	Sumoylation		Uniprot
K197	Ubiquitination		Uniprot
Y201	Phosphorylation		Uniprot
S210	Phosphorylation		Uniprot
K218	Acetylation		Uniprot
K218	Methylation		Uniprot
K218	Ubiquitination		Uniprot
C226	S-Nitrosylation		Uniprot
K231	Acetylation		Uniprot
K231	Ubiquitination		Uniprot
K238	Acetylation		Uniprot
K243	Acetylation		Uniprot
K243	Ubiquitination		Uniprot
Y244	Phosphorylation		Uniprot
K251	Acetylation		Uniprot
K251	Ubiquitination		Uniprot
K255	Ubiquitination		Uniprot
K260	Methylation		Uniprot
K260	Ubiquitination		Uniprot
Y263	Phosphorylation		Uniprot
S271	Phosphorylation		Uniprot
R272	Methylation		Uniprot
R278	Methylation		Uniprot
R280	Methylation		Uniprot
R282	Methylation		Uniprot
R345	Methylation		Uniprot
S351	Phosphorylation		Uniprot
K353	Acetylation		Uniprot
K353	Methylation		Uniprot

Research Backgrounds

Q14103_HNRPD_HUMAN

Function:

Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Binding of RRM1 to DNA inhibits the formation of DNA quadruplex structure which may play a role in telomere elongation. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. May play a role in the regulation of the rhythmic expression of circadian clock core genes. Directly binds to the 3'UTR of CRY1 mRNA and induces CRY1 rhythmic translation. May also be involved in the regulation of PER2 translation.

PTMs:

Arg-345 is dimethylated, probably to asymmetric dimethylarginine.

Methylated by PRMT1, in an insulin-dependent manner. The PRMT1-mediated methylation regulates tyrosine phosphorylation (By similarity).

Subcellular Location:

Nucleus. Cytoplasm.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Cytoplasmic localization oscillates diurnally.

Subunit Structure:

Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Part of a complex associated with the FOS mCRD domain and consisting of PABPC1, PAIP1, CSDE1/UNR and SYNCRIP. Interacts with IGF2BP2. Interacts with GTPBP1. Interacts with EIF4G1; the interaction requires RNA. Interacts with EIF3B and RPS3.

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Phospho-hnRPD (Ser83) Antibody - #AF0033