Phospho-CDC25A (Ser76) Antibody | Affinity Biosciences

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AF0021 at 1/100 staining Mouse spleen tissue by IHC-P.

Product:	Phospho-CDC25A (Ser76) Antibody
Catalog:	AF0021
Description:	Rabbit polyclonal antibody to Phospho-CDC25A (Ser76)
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	59kDa; 59kD(Calculated).
Uniprot:	P30304
RRID:	AB_2834083

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(88%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(88%), Dog(88%)

Clonality:

Polyclonal

Specificity:

Phospho-CDC25A (Ser76) Antibody detects endogenous levels of CDC25A only when phosphorylated at Sersine 76.

RRID:

AB_2834083
Cite Format: Affinity Biosciences Cat# AF0021, RRID:AB_2834083.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Cdc 25a; CDC25A; CDC25A2; CDC25A2 CAG isoform; Cell division cycle 25 homolog A (S. pombe); Cell division cycle 25A; Cell division cycle 25A isoform a; Cell division cycle 25A isoform b; D9Ertd393e; Dual specificity phosphatase Cdc25A; M phase inducer phosphatase 1; M-phase inducer phosphatase 1; MGC115549; MPIP1_HUMAN;

Immunogens

Immunogen:

Uniprot:

P30304(MPIP1_HUMAN) >>Visit HPA database.

Gene(ID):

CDC25A(993)

Description:

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.

Sequence:

P30304 MPIP1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MELGPEPPHRRRLLFACSPPPASQPVVKALFGASAAGGLSPVTNLTVTMDQLQGLGSDYEQPLEVKNNSNLQRMGSSESTDSGFCLDSPGPLDSKENLENPMRRIHSLPQKLLGCSPALKRSHSDSLDHDIFQLIDPDENKENEAFEFKKPVRPVSRGCLHSHGLQEGKDLFTQRQNSAPARMLSSNERDSSEPGNFIPLFTPQSPVTATLSDEDDGFVDLLDGENLKNEEETPSCMASLWTAPLVMRTTNLDNRCKLFDSPSLCSSSTRSVLKRPERSQEESPPGSTKRRKSMSGASPKESTNPEKAHETLHQSLSLASSPKGTIENILDNDPRDLIGDFSKGYLFHTVAGKHQDLKYISPEIMASVLNGKFANLIKEFVIIDCRYPYEYEGGHIKGAVNLHMEEEVEDFLLKKPIVPTDGKRVIVVFHCEFSSERGPRMCRYVRERDRLGNEYPKLHYPELYVLKGGYKEFFMKCQSYCEPPSYRPMHHEDFKEDLKKFRTKSRTWAGEKSKREMYSRLKKL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Sheep

Dog

Rabbit

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30304 As Substrate

P30304

Site	PTM Type	Enzyme	Source
	Ubiquitination		Uniprot
S18	Phosphorylation	P06493 (CDK1)	Uniprot
S40	Phosphorylation		Uniprot
Y59	Phosphorylation		Uniprot
S76	Phosphorylation	O14757 (CHEK1) , P49841 (GSK3B)	Uniprot
S77	Phosphorylation		Uniprot
S79	Phosphorylation	Q8NG66 (NEK11) , P48729 (CSNK1A1)	Uniprot
T80	Phosphorylation	Q9H4B4 (PLK3)	Uniprot
S82	Phosphorylation	P48729 (CSNK1A1) , Q8NG66 (NEK11)	Uniprot
S88	Phosphorylation	Q8NG66 (NEK11)	Uniprot
S107	Phosphorylation		Uniprot
K111	Ubiquitination		Uniprot
S116	Phosphorylation	P06493 (CDK1)	Uniprot
K120	Ubiquitination		Uniprot
S124	Phosphorylation	Q683Z8 (CHK2) , O96017 (CHEK2) , O14757 (CHEK1)	Uniprot
S126	Phosphorylation		Uniprot
K150	Acetylation		Uniprot
K150	Ubiquitination		Uniprot
K169	Ubiquitination		Uniprot
S178	Phosphorylation	Q683Z8 (CHK2) , O14757 (CHEK1) , O96017 (CHEK2)	Uniprot
K257	Ubiquitination		Uniprot
S261	Phosphorylation		Uniprot
S263	Phosphorylation	P24941 (CDK2)	Uniprot
R278	Methylation		Uniprot
S279	Phosphorylation	Q683Z8 (CHK2) , O14757 (CHEK1) , O96017 (CHEK2)	Uniprot
S283	Phosphorylation		Uniprot
R290	Methylation		Uniprot
S293	Phosphorylation	O14757 (CHEK1) , O96017 (CHEK2) , Q15418 (RPS6KA1) , Q683Z8 (CHK2)	Uniprot
S295	Phosphorylation	Q15418 (RPS6KA1)	Uniprot
K300	Ubiquitination		Uniprot
S321	Phosphorylation		Uniprot
K343	Ubiquitination		Uniprot
K353	Ubiquitination		Uniprot
K358	Ubiquitination		Uniprot
Y359	Phosphorylation		Uniprot
S361	Phosphorylation		Uniprot
S367	Phosphorylation		Uniprot
K372	Ubiquitination		Uniprot
K378	Ubiquitination		Uniprot
K415	Ubiquitination		Uniprot
K471	Ubiquitination		Uniprot
Y486	Phosphorylation		Uniprot
T507	Phosphorylation	O14757 (CHEK1)	Uniprot
S513	Phosphorylation	Q9H4B4 (PLK3)	Uniprot
Y518	Phosphorylation		Uniprot
S519	Phosphorylation	Q9H4B4 (PLK3)	Uniprot

Research Backgrounds

P30304_MPIP1_HUMAN

Function:

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

PTMs:

Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation.

Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.

Subunit Structure:

Interacts with CCNB1/cyclin B1. Interacts with YWHAE/14-3-3 epsilon when phosphorylated. Interacts with CUL1 specifically when CUL1 is neddylated and active. Interacts with BTRC/BTRCP1 and FBXW11/BTRCP2. Interactions with CUL1, BTRC and FBXW11 are enhanced upon DNA damage. Interacts with PIM1. Interacts with CHEK2; mediates CDC25A phosphorylation and degradation in response to infrared-induced DNA damages. Interacts with HSP90AB1; prevents heat shock-mediated CDC25A degradation and contributes to cell cycle progression.

Family&Domains:

The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.

Belongs to the MPI phosphatase family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

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Phospho-CDC25A (Ser76) Antibody - #AF0021