Product: Phospho-PYK2 (Tyr580) Antibody
Catalog: AF0020
Source: Rabbit
Application: WB, IHC, ELISA(peptide)
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 135kD; 116kD(Calculated).
Uniprot: Q14289
RRID: AB_2834082

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(88%), Dog(88%), Chicken(88%)
Clonality:
Polyclonal
Specificity:
Phospho-PYK2 (Tyr580) Antibody detects endogenous levels of PYK2 only when phosphorylated at Tyrosine 580.
RRID:
AB_2834082
Cite Format: Affinity Biosciences Cat# AF0020, RRID:AB_2834082.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CADTK; CAK-beta; CAKB; CAKbeta; Calcium regulated non receptor proline rich tyrosine kinase; Calcium-dependent tyrosine kinase; Cell adhesion kinase beta; E430023O05Rik; EC 2.7.10.2; FADK 2; FADK2; FAK2; FAK2_HUMAN; Focal adhesion kinase 2; MGC124628; PKB; Proline-rich tyrosine kinase 2; Protein kinase B; Protein Tyrosine Kinase 2 Beta; Protein-tyrosine kinase 2-beta; PTK; PTK2B; PTK2B protein tyrosine kinase 2 beta; PYK2; RAFTK; RAFTK2; Related adhesion focal tyrosine kinase;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q14289 FAK2_HUMAN:

Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney (at protein level). Also expressed in spleen and lymphocytes.

Description:
Pyk2 a nonreceptor tyrosine kinase of the Fak family. Predominantly expressed in the cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for G-protein-coupled receptors. Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. Interacts with the SH2 domain of Grb2.
Sequence:
MSGVSEPLSRVKLGTLRRPEGPAEPMVVVPVDVEKEDVRILKVCFYSNSFNPGKNFKLVKCTVQTEIREIITSILLSGRIGPNIRLAECYGLRLKHMKSDEIHWLHPQMTVGEVQDKYECLHVEAEWRYDLQIRYLPEDFMESLKEDRTTLLYFYQQLRNDYMQRYASKVSEGMALQLGCLELRRFFKDMPHNALDKKSNFELLEKEVGLDLFFPKQMQENLKPKQFRKMIQQTFQQYASLREEECVMKFFNTLAGFANIDQETYRCELIQGWNITVDLVIGPKGIRQLTSQDAKPTCLAEFKQIRSIRCLPLEEGQAVLQLGIEGAPQALSIKTSSLAEAENMADLIDGYCRLQGEHQGSLIIHPRKDGEKRNSLPQIPMLNLEARRSHLSESCSIESDIYAEIPDETLRRPGGPQYGIAREDVVLNRILGEGFFGEVYEGVYTNHKGEKINVAVKTCKKDCTLDNKEKFMSEAVIMKNLDHPHIVKLIGIIEEEPTWIIMELYPYGELGHYLERNKNSLKVLTLVLYSLQICKAMAYLESINCVHRDIAVRNILVASPECVKLGDFGLSRYIEDEDYYKASVTRLPIKWMSPESINFRRFTTASDVWMFAVCMWEILSFGKQPFFWLENKDVIGVLEKGDRLPKPDLCPPVLYTLMTRCWDYDPSDRPRFTELVCSLSDVYQMEKDIAMEQERNARYRTPKILEPTAFQEPPPKPSRPKYRPPPQTNLLAPKLQFQVPEGLCASSPTLTSPMEYPSPVNSLHTPPLHRHNVFKRHSMREEDFIQPSSREEAQQLWEAEKVKMRQILDKQQKQMVEDYQWLRQEEKSLDPMVYMNDKSPLTPEKEVGYLEFTGPPQKPPRLGAQSIQPTANLDRTDDLVYLNVMELVRAVLELKNELCQLPPEGYVVVVKNVGLTLRKLIGSVDDLLPSLPSSSRTEIEGTQKLLNKDLAELINKMRLAQQNAVTSLSEECKRQMLTASHTLAVDAKNLLDAVDQAKVLANLAHPPAE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Horse
88
Bovine
88
Sheep
88
Dog
88
Chicken
88
Rabbit
88
Xenopus
75
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14289 As Substrate

Site PTM Type Enzyme
Phosphorylation
S2 Phosphorylation
S9 Phosphorylation
K12 Ubiquitination
T15 Phosphorylation
K54 Ubiquitination
K188 Ubiquitination
K198 Ubiquitination
K206 Ubiquitination
K216 Ubiquitination
K295 Ubiquitination
K303 Ubiquitination
S332 Phosphorylation
S361 Phosphorylation
S375 Phosphorylation
S394 Phosphorylation
S396 Phosphorylation
S399 Phosphorylation
Y402 Phosphorylation Q14289 (PTK2B) , P43405 (SYK) , P12931 (SRC)
T409 Phosphorylation
Y418 Phosphorylation
Y440 Phosphorylation
K451 Ubiquitination
K457 Ubiquitination
S473 Phosphorylation
K479 Ubiquitination
S530 Phosphorylation
S559 Phosphorylation
K564 Acetylation
K564 Ubiquitination
S571 Phosphorylation
Y573 Phosphorylation
Y579 Phosphorylation
Y580 Phosphorylation
K581 Ubiquitination
S583 Phosphorylation
K640 Ubiquitination
Y683 Phosphorylation
Y699 Phosphorylation
T701 Phosphorylation
K703 Ubiquitination
S718 Phosphorylation
Y722 Phosphorylation
S746 Phosphorylation
S747 Phosphorylation
T749 Phosphorylation
S752 Phosphorylation
Y756 Phosphorylation
S758 Phosphorylation
S762 Phosphorylation
T765 Phosphorylation
S778 Phosphorylation
K801 Ubiquitination
K810 Ubiquitination
Y819 Phosphorylation
S828 Phosphorylation
Y834 Phosphorylation
S839 Phosphorylation
T842 Phosphorylation
Y849 Phosphorylation
T853 Phosphorylation
K858 Ubiquitination
S866 Phosphorylation
Y881 Phosphorylation P12931 (SRC)
Y906 Phosphorylation
K911 Ubiquitination
S923 Phosphorylation
K944 Ubiquitination
K948 Ubiquitination
K956 Ubiquitination
K973 Ubiquitination
K998 Ubiquitination

PTMs - Q14289 As Enzyme

Substrate Site Source
O15259 (NPHP1) Y46 Uniprot
O15259 (NPHP1) Y349 Uniprot
O15259 (NPHP1) Y721 Uniprot
O43294 (TGFB1I1) Y60 Uniprot
P29474 (NOS3) Y657 Uniprot
P37840 (SNCA) Y125 Uniprot
Q05397 (PTK2) Y407 Uniprot
Q14289-1 (PTK2B) S375 Uniprot
Q14289-1 (PTK2B) Y402 Uniprot
Q14289-2 (PTK2B) Y579 Uniprot
Q14289-1 (PTK2B) Y580 Uniprot
Q14289-1 (PTK2B) S778 Uniprot
Q14289-2 (PTK2B) Y839 Uniprot
Q14289-1 (PTK2B) Y881 Uniprot
Q658W2 (DKFZp666O0110) Y429 Uniprot
Q9ULH1 (ASAP1) Y767 Uniprot
Q9ULZ3 (PYCARD) Y146 Uniprot

Research Backgrounds

Function:

Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376'. Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca(2+) levels. May also regulate potassium ion transport by phosphorylation of potassium channel subunits. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN; this requires both SRC and PTK2/PYK2.

PTMs:

Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration; this activation is indirect and may be mediated by production of reactive oxygen species (ROS). Tyr-402 is the major autophosphorylation site, but other kinases can also phosphorylate Tyr-402. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-402 promotes interaction with SRC and SRC family members, leading to phosphorylation at Tyr-579; Tyr-580 and Tyr-881. Phosphorylation at Tyr-881 is important for interaction with GRB2. Phosphorylated on tyrosine residues upon activation of FGR and PKC. Recruitment by NPHP1 to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity. Phosphorylation by MYLK promotes ITGB2 activation and is thus essential to trigger neutrophil transmigration during lung injury. Dephosphorylated by PTPN12.

Subcellular Location:

Cytoplasm. Cytoplasm>Perinuclear region. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell junction>Focal adhesion. Cell projection>Lamellipodium. Cytoplasm>Cell cortex. Nucleus.
Note: Interaction with NPHP1 induces the membrane-association of the kinase. Colocalizes with integrins at the cell periphery.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney (at protein level). Also expressed in spleen and lymphocytes.

Subunit Structure:

Homodimer, or homooligomer. Interacts with SIRPA and SH2D3C. Interacts with ARHGAP10. Interacts with DLG4 (By similarity). Interacts with KCNA2 (By similarity). Interacts with NPHP1, ASAP1, ASAP2, ARHGAP26, SKAP2 and TGFB1I1. The Tyr-402 phosphorylated form interacts with SRC (via SH2 domain) and SRC family members. Forms a signaling complex with EPHA1, LCK and phosphatidylinositol 3-kinase; upon activation by EFNA1. Interacts with GRB2 (via SH2 domain). Interacts with P53/TP53 and MDM2. Interacts with MYLK. Interacts with BCAR1. Interacts with PDPK1. Interacts (hypophosphorylated) with PXN. Interacts with RB1CC1. Interacts with RHOU. Interacts with VAV1. Interacts with LPXN and PTPN12.

Family&Domains:

Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

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