ITGB8 Antibody | Affinity Biosciences

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Product:	ITGB8 Antibody
Catalog:	DF2545
Description:	Rabbit polyclonal antibody to ITGB8
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	81 kDa; 86kD(Calculated).
Uniprot:	P26012
RRID:	AB_2839751

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(89%), Dog(100%)

Clonality:

Polyclonal

Specificity:

ITGB8 Antibody detects endogenous levels of total ITGB8.

RRID:

AB_2839751
Cite Format: Affinity Biosciences Cat# DF2545, RRID:AB_2839751.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Integrin beta-8; ITB8_HUMAN; ITGB 8; ITGB8;

Immunogens

Immunogen:

Uniprot:

P26012(ITB8_HUMAN) >>Visit HPA database.

Gene(ID):

ITGB8(3696)

Expression:

P26012 ITB8_HUMAN:

Placenta, kidney, brain, ovary, uterus and in several transformed cells. Transiently expressed in 293 human embryonic kidney cells.

Description:

Integrin alpha-V/beta-8 is a receptor for fibronectin.

Sequence:

P26012 ITB8_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MCGSALAFFTAAFVCLQNDRRGPASFLWAAWVFSLVLGLGQGEDNRCASSNAASCARCLALGPECGWCVQEDFISGGSRSERCDIVSNLISKGCSVDSIEYPSVHVIIPTENEINTQVTPGEVSIQLRPGAEANFMLKVHPLKKYPVDLYYLVDVSASMHNNIEKLNSVGNDLSRKMAFFSRDFRLGFGSYVDKTVSPYISIHPERIHNQCSDYNLDCMPPHGYIHVLSLTENITEFEKAVHRQKISGNIDTPEGGFDAMLQAAVCESHIGWRKEAKRLLLVMTDQTSHLALDSKLAGIVVPNDGNCHLKNNVYVKSTTMEHPSLGQLSEKLIDNNINVIFAVQGKQFHWYKDLLPLLPGTIAGEIESKAANLNNLVVEAYQKLISEVKVQVENQVQGIYFNITAICPDGSRKPGMEGCRNVTSNDEVLFNVTVTMKKCDVTGGKNYAIIKPIGFNETAKIHIHRNCSCQCEDNRGPKGKCVDETFLDSKCFQCDENKCHFDEDQFSSESCKSHKDQPVCSGRGVCVCGKCSCHKIKLGKVYGKYCEKDDFSCPYHHGNLCAGHGECEAGRCQCFSGWEGDRCQCPSAAAQHCVNSKGQVCSGRGTCVCGRCECTDPRSIGRFCEHCPTCYTACKENWNCMQCLHPHNLSQAILDQCKTSCALMEQQHYVDQTSECFSSPSYLRIFFIIFIVTFLIGLLKVLIIRQVILQWNSNKIKSSSDYRVSASKKDKLILQSVCTRAVTYRREKPEEIKMDISKLNAHETFRCNF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

Chicken

Zebrafish

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P26012 As Substrate

P26012

Site	PTM Type	Source
S75	Phosphorylation	Uniprot
S78	Phosphorylation	Uniprot
Y314	Phosphorylation	Uniprot
S329	Phosphorylation	Uniprot
Y447	Phosphorylation	Uniprot
S468	Phosphorylation	Uniprot
K480	Ubiquitination	Uniprot
K758	Ubiquitination	Uniprot

Research Backgrounds

P26012_ITB8_HUMAN

Function:

Integrin alpha-V:beta-8 (ITGAV:ITGB8) is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligands. Integrin alpha-V:beta-6 (ITGAV:ITGB6) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation on the surface of activated regulatory T-cells (Tregs) (Probable). Required during vasculogenesis (By similarity).

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Tissue Specificity:

Placenta, kidney, brain, ovary, uterus and in several transformed cells. Transiently expressed in 293 human embryonic kidney cells.

Subunit Structure:

Heterodimer of an alpha and a beta subunit. Beta-8 (ITGB8) associates with alpha-V (ITGAV) to form ITGAV:ITGB8. ITGAV:ITGB8 interacts with TGFB1.

Family&Domains:

Belongs to the integrin beta chain family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

References

1). The Role of YB1 in Renal Cell Carcinoma Cell Adhesion. International Journal of Medical Sciences (PubMed: 30275756) [IF=3.6]

Application: WB Species: human Sample: 786-0-shYB1 cells

Figure 2. |ITGB8 was significantly down-regulated in 786-0-shYB1 cells. A. The reliability of the microarray data was further verified by real-time PCR analysis. B. Western blotting analysis showing ITGB8 and YB1 protein levels in 786-0-shYB1 and control cells. (** indicate p < 0.01)

2). Long Noncoding RNA CTD-2589M5.4 Inhibits Ovarian Cancer Cell Proliferation, Migration, and Invasion Via Downregulation of the Extracellular Matrix–Receptor Interaction Pathway. Cancer biotherapy & radiopharmaceuticals (PubMed: 34242057) [IF=3.4]

3). RETRACTED: Long Noncoding RNA CTD-2589M5.4 Inhibits Ovarian Cancer Cell Proliferation, Migration, and Invasion Via Downregulation of the Extracellular Matrix–Receptor Interaction Pathway. Cancer Biotherapy & Radiopharmaceuticals (PubMed: 34242057) [IF=3.4]

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ITGB8 Antibody - #DF2545