ITGA1 Antibody | Affinity Biosciences

WB
IF/ICC
Cites

1/5

Western blot analysis of extracts from Mouse testis, using ITGA1 Antibody at 1/1000 dilution.

DF2538 staining H2O2 treated Hela cells by IF/ICC.

DF2538 staining Hela cells by IF/ICC.

Fig.

Figure 5 Measurement of expression levels of DEPs, oxidative stress markers and proinflammatory cytokines production.

Product:	ITGA1 Antibody
Catalog:	DF2538
Description:	Rabbit polyclonal antibody to ITGA1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.:	131 kDa; 131kD(Calculated).
Uniprot:	P56199
RRID:	AB_2839744

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

ITGA1 Antibody detects endogenous levels of total ITGA1.

RRID:

AB_2839744
Cite Format: Affinity Biosciences Cat# DF2538, RRID:AB_2839744.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD 49a; CD49 antigen-like family member A; CD49a; CD49a antigen; Integrin alpha-1; ITA1_HUMAN; Itga 1; ITGA1; Laminin and collagen receptor; Very late activation protein 1; VLA 1; VLA-1; VLA1;

Immunogens

Immunogen:

Uniprot:

P56199(ITA1_HUMAN) >>Visit HPA database.

Gene(ID):

ITGA1(3672)

Description:

Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen.

Sequence:

P56199 ITA1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAPRPRARPGVAVACCWLLTVVLRCCVSFNVDVKNSMTFSGPVEDMFGYTVQQYENEEGKWVLIGSPLVGQPKNRTGDVYKCPVGRGESLPCVKLDLPVNTSIPNVTEVKENMTFGSTLVTNPNGGFLACGPLYAYRCGHLHYTTGICSDVSPTFQVVNSIAPVQECSTQLDIVIVLDGSNSIYPWDSVTAFLNDLLERMDIGPKQTQVGIVQYGENVTHEFNLNKYSSTEEVLVAAKKIVQRGGRQTMTALGIDTARKEAFTEARGARRGVKKVMVIVTDGESHDNHRLKKVIQDCEDENIQRFSIAILGSYNRGNLSTEKFVEEIKSIASEPTEKHFFNVSDELALVTIVKTLGERIFALEATADQSAASFEMEMSQTGFSAHYSQDWVMLGAVGAYDWNGTVVMQKASQIIIPRNTTFNVESTKKNEPLASYLGYTVNSATASSGDVLYIAGQPRYNHTGQVIIYRMEDGNIKILQTLSGEQIGSYFGSILTTTDIDKDSNTDILLVGAPMYMGTEKEEQGKVYVYALNQTRFEYQMSLEPIKQTCCSSRQHNSCTTENKNEPCGARFGTAIAAVKDLNLDGFNDIVIGAPLEDDHGGAVYIYHGSGKTIRKEYAQRIPSGGDGKTLKFFGQSIHGEMDLNGDGLTDVTIGGLGGAALFWSRDVAVVKVTMNFEPNKVNIQKKNCHMEGKETVCINATVCFDVKLKSKEDTIYEADLQYRVTLDSLRQISRSFFSGTQERKVQRNITVRKSECTKHSFYMLDKHDFQDSVRITLDFNLTDPENGPVLDDSLPNSVHEYIPFAKDCGNKEKCISDLSLHVATTEKDLLIVRSQNDKFNVSLTVKNTKDSAYNTRTIVHYSPNLVFSGIEAIQKDSCESNHNITCKVGYPFLRRGEMVTFKILFQFNTSYLMENVTIYLSATSDSEEPPETLSDNVVNISIPVKYEVGLQFYSSASEYHISIAANETVPEVINSTEDIGNEINIFYLIRKSGSFPMPELKLSISFPNMTSNGYPVLYPTGLSSSENANCRPHIFEDPFSINSGKKMTTSTDHLKRGTILDCNTCKFATITCNLTSSDISQVNVSLILWKPTFIKSYFSSLNLTIRGELRSENASLVLSSSNQKRELAIQISKDGLPGRVPLWVILLSAFAGLLLLMLLILALWKIGFFKRPLKKKMEK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Zebrafish

Horse

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P56199 As Substrate

P56199

Site	PTM Type	Source
K81	Ubiquitination	Uniprot
N100	N-Glycosylation	Uniprot
N105	N-Glycosylation	Uniprot
N217	N-Glycosylation	Uniprot
K274	Acetylation	Uniprot
K328	Ubiquitination	Uniprot
N341	N-Glycosylation	Uniprot
N418	N-Glycosylation	Uniprot
T419	Phosphorylation	Uniprot
T420	Phosphorylation	Uniprot
N460	N-Glycosylation	Uniprot
Y527	Phosphorylation	Uniprot
N532	N-Glycosylation	Uniprot
S541	Phosphorylation	Uniprot
Y604	Phosphorylation	Uniprot
T612	Phosphorylation	Uniprot
K709	Ubiquitination	Uniprot
N780	N-Glycosylation	Uniprot
S834	Phosphorylation	Uniprot
N840	N-Glycosylation	Uniprot
Y890	Phosphorylation	Uniprot
N966	N-Glycosylation	Uniprot
N974	N-Glycosylation	Uniprot
S992	Phosphorylation	Uniprot
S994	Phosphorylation	Uniprot
N1008	N-Glycosylation	Uniprot
K1046	Ubiquitination	Uniprot

Research Backgrounds

P56199_ITA1_HUMAN

Function:

Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Involved in anchorage-dependent, negative regulation of EGF-stimulated cell growth.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Subunit Structure:

Heterodimer of an alpha and a beta subunit. Alpha-1 associates with beta-1. Interacts with RAB21. Interacts (via cytoplasmic domain) with PTPN2; activates PTPN2 phosphatase activity towards EGFR and negatively regulates EGF signaling.

Family&Domains:

The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.

Belongs to the integrin alpha chain family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction. (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)

References

1). SERPINA5 Protein in Cumulus-Oocyte Complexes Increases the Fertilisation Ability of Mouse Sperm. Reproductive Sciences (PubMed: 35194761) [IF=2.9]

2). Verification of osteoblast differentiation on airborne-particle abrasion, large-grit, acid-etched surface of titanium implants regulated by yes-associated protein and transcriptional coactivator with PDZ-binding motif. Journal of Oral Science (PubMed: 31327805) [IF=1.9]

Application: WB Species: mouse Sample: MC3T3-E1 cells

Fig. 5 | mRNA and protein expression of integrins α1, α2, and β1 on Polished Ti and SLA Ti: (a) mRNA expression after GAPDH standardization of integrin α1, integrin α2, and integrin β1 on Polished Ti and SLA Ti, detected with qRT-PCR on the 3rd day; (b) protein expression of integrin α1, integrin α2, and integrin β1 on Polished Ti and SLA Ti, detected with Western blot on the 3rd day; *P < 0.05 when SLA Ti and Polished Ti are compared; **P < 0.01 is considered statistically significant when SLA Ti and Polished Ti are compared; ***P < 0.0001 is considered statistically significant when SLA Ti and Polished Ti are compared.

3). TMT-Based Quantitative Proteomics Reveals Cochlear Protein Profile Alterations in Mice with Noise-Induced Hearing Loss. International Journal of Environmental Research and Public Health (PubMed: 35010640)

Application: WB Species: Mice Sample:

Figure 5 Measurement of expression levels of DEPs, oxidative stress markers and proinflammatory cytokines production. (a) Validation of representative inflammation and autophagy-related DEPs by western blots in the control group (n = 3) and noise group (n = 3). (b) Comparison of MDA level and SOD activity in cochlea between the control group (n = 3) and noise group (n = 3). (c) Comparison of proinflammatory cytokines TNF-α and IL-6 in cochlea between the control group (n = 3) and noise group (n = 3). Data are represented as mean ± SD. * p < 0.05, ** p < 0.01.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

ITGA1 Antibody - #DF2538