CPEB1 Antibody | Affinity Biosciences

WB
IF/ICC
Cites

1/3

Western blot analysis of extracts from various samples, using CPEB1 Antibody.

Figure 4 Targeted regulation of CPEB1 expression by miR-301.

Product:	CPEB1 Antibody
Catalog:	DF2462
Description:	Rabbit polyclonal antibody to CPEB1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	63 kDa; 63kD(Calculated).
Uniprot:	Q9BZB8
RRID:	AB_2839668

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

CPEB1 Antibody detects endogenous levels of total CPEB1.

RRID:

AB_2839668
Cite Format: Affinity Biosciences Cat# DF2462, RRID:AB_2839668.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CEBP; CPE binding protein 1; CPE BP1; CPE-binding protein 1; CPE-BP1; CPEB 1; CPEB; CPEB-1; CPEB1; CPEB1 protein; CPEB1_HUMAN; Cytoplasmic polyadenylation binding protein 1; Cytoplasmic polyadenylation element binding protein; Cytoplasmic polyadenylation element binding protein 1; Cytoplasmic polyadenylation element-binding protein 1; FLJ13203; h CEBP; h-CEBP; hCPEB 1; hCPEB-1; MGC34136; MGC60106; mKIAA0940; MKIAA0940 protein;

Immunogens

Immunogen:

Uniprot:

Q9BZB8(CPEB1_HUMAN) >>Visit HPA database.

Gene(ID):

CPEB1(64506)

Expression:

Q9BZB8 CPEB1_HUMAN:

Isoform 1 is expressed in immature oocytes, ovary, brain and heart. Isoform 2 is expressed in brain and heart. Isoform 3 and isoform 4 are expressed in brain. Expressed in breast tumors and several tumor cell lines.

Description:

Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR. In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation (By similarity). Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses (By similarity). Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation (By similarity). Induces the assembly of stress granules in the absence of stress.

Sequence:

Q9BZB8 CPEB1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MALSLEEEAGRIKDCWDNQEAPALSTCSNANIFRRINAILDNSLDFSRVCTTPINRGIHDHLPDFQDSEETVTSRMLFPTSAQESSRGLPDANDLCLGLQSLSLTGWDRPWSTQDSDSSAQSSTHSVLSMLHNPLGNVLGKPPLSFLPLDPLGSDLVDKFPAPSVRGSRLDTRPILDSRSSSPSDSDTSGFSSGSDHLSDLISSLRISPPLPFLSLSGGGPRDPLKMGVGSRMDQEQAALAAVTPSPTSASKRWPGASVWPSWDLLEAPKDPFSIEREARLHRQAAAVNEATCTWSGQLPPRNYKNPIYSCKVFLGGVPWDITEAGLVNTFRVFGSLSVEWPGKDGKHPRCPPKGNMPKGYVYLVFELEKSVRSLLQACSHDPLSPDGLSEYYFKMSSRRMRCKEVQVIPWVLADSNFVRSPSQRLDPSRTVFVGALHGMLNAEALAAILNDLFGGVVYAGIDTDKHKYPIGSGRVTFNNQRSYLKAVSAAFVEIKTTKFTKKVQIDPYLEDSLCHICSSQPGPFFCRDQVCFKYFCRSCWHWRHSMEGLRHHSPLMRNQKNRDSS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Chicken

100

Rabbit

100

Bovine

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BZB8 As Substrate

Q9BZB8

Site	PTM Type	Source
K13	Ubiquitination	Uniprot
S43	Phosphorylation	Uniprot
T52	Phosphorylation	Uniprot
T80	Phosphorylation	Uniprot
T172	Phosphorylation	Uniprot
S178	Phosphorylation	Uniprot
S184	Phosphorylation	Uniprot
S208	Phosphorylation	Uniprot
K226	Ubiquitination	Uniprot
S231	Phosphorylation	Uniprot
K252	Ubiquitination	Uniprot
K305	Ubiquitination	Uniprot
K347	Acetylation	Uniprot

Research Backgrounds

Q9BZB8_CPEB1_HUMAN

Function:

Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR. RNA binding results in a clear conformational change analogous to the Venus fly trap mechanism. In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation (By similarity). Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses (By similarity). Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation (By similarity). Induces the assembly of stress granules in the absence of stress. Required for cell cycle progression, specifically for prophase entry.

PTMs:

Phosphorylated on serine/threonine residues by AURKA within positions 166 and 197. Phosphorylation and dephosphorylation on Thr-172 regulates cytoplasmic polyadenylation and translation of CPE-containing mRNAs. Phosphorylation on Thr-172 by AURKA and CAMK2A activates CPEB1. Phosphorylation on Thr-172 may be promoted by APLP1. Phosphorylation increases binding to RNA (By similarity).

Subcellular Location:

Cytoplasm. Nucleus. Cytoplasm>P-body. Cytoplasmic granule. Cell junction>Synapse. Membrane. Cell junction>Synapse>Postsynaptic density. Cell projection>Dendrite.
Note: Continuously shuttling between nucleus and cytoplasm (PubMed:18923137). Also found in stress granules. Recruited to stress granules (SGs) upon arsenite treatment. In dendrites (By similarity). Localizes in synaptosomes at dendritic synapses of neurons (By similarity). Strongly enriched in postsynaptic density (PSD) fractions (By similarity). Transported into dendrites in a microtubule-dependent fashion and colocalizes in mRNA-containing particles with TACC3, dynein and kinesin (By similarity). Membrane-associated (By similarity). Colocalizes at excitatory synapses with members of the polyadenylation and translation complex factors (CPSF, APLP1, TACC3, AURKA, SYP, etc.) including CPE-containing RNAs (By similarity).

Tissue Specificity:

Subunit Structure:

Interacts with kinesin, dynein, APLP1, APLP2, TENT2/GLD2 and APP. Both phosphorylated and non phosphorylated forms interact with APLP1 (By similarity). Interacts with TENT4B; the interaction is required for TENT4B-mediated translational control.

Family&Domains:

The 2 RRM domains and the C-terminal region mediate interaction with CPE-containing RNA (PubMed:24990967). The interdomain linker (411-429) acts as a hinge to fix the relative orientation of the 2 RRMs (PubMed:24990967). The ZZ domain (509-566) coordinates 2 Zn ions and is probably implicated in mediating interactions with other proteins in addition to increasing the affinity of the RRMs for the CPEs (PubMed:23500490, PubMed:24990967). A continuous hydrophobic interface is formed between the 2 RRMs (PubMed:24990967).

Belongs to the RRM CPEB family.

Research Fields

· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

References

1). MiR-301 regulates the SIRT1/SOX2 pathway via CPEB1 in the breast cancer progression. Molecular Therapy-Oncolytics (PubMed: 34377766) [IF=5.7]

Application: WB Species: Human Sample: breast cancer cells

Figure 4 Targeted regulation of CPEB1 expression by miR-301. A: Detection results of fluorescence activity of miR-301 and CPEB1; B: RIP experiment to detect the combination of miR-301, CPEB1 and Ago2; C: Detection of CPEB1 expression in cancer and para-cancerous tissues by RT-qPCR; D: Correlation Analysis of miR-301 and CPEB1; E: The expression of CPEB1 in each group of cells was tested by RT-qPCR; F: Western blot was used to detect the protein expression of CPEB1 in each group of cells. The continuous variables were expressed by mean ± SD (standard deviation); * P < 0.05 compared with normal tissues or normal cells; paired sample t-test, independent sample t-test or one-way ANOVA were used to compare the differences of continuous variables among different groups; Pearson correlation is used to analyze the correlation between CPEB1 and miR-301. We repeated the experiment three times to get accurate and stable results.

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CPEB1 Antibody - #DF2462