Goat Anti-Mouse IgG (H+L) HRP
Price Size
$250 50ul
$350 100ul
$450 200ul

Same day delivery

Contact distributor
  • Product Name
    Goat Anti-Mouse IgG (H+L) HRP
  • Catalog No.
    S0002
  • RRID
    AB_2839430
  • Source
    Goat
  • Application
    WB,IHC
  • Reactivity
    Mouse
  • Mol.Wt

Product Information

Applications:

WB 1:3000~1:10000, IHC 1:200
*The optimal dilutions should be determined by the end user.

Reactivity:

Mouse

Source:

Goat

Clonality:

Polyclonal

Purification:

affinity purification.

RRID:

AB_2839430
Please cite this product as: Affinity Biosciences Cat# S0002, RRID:AB_2839430.

Storage Condition and Buffer:

HRP-labeled Goat IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl.

Immunogen Information

Immunogen:

The antiserum was developed in goat using the mouse IgG as the immunogen.

Molecular Weight:

Observed Mol.Wt.: Not available.
Predicted Mol.Wt.: Not available.

Not available

Reference Citations:

1). Lei R et al. bHLH121 Functions as A Direct Link that Facilitates the Activation of FIT by bHLH IVc Transcription Factors for Maintaining Fe Homeostasis in Arabidopsis. Mol Plant 2020 Jan 18 (PubMed: 31962167) [IF=12.084]

2). Bian WX et al. Binding of cellular nucleolin with the viral core RNA G-quadruplex structure suppresses HCV replication. Nucleic Acids Res 2018 Nov 20 (PubMed: 30462330) [IF=11.501]

Application: WB    Species:human;    Sample:hepatoma cell lines

(B) G4 pull-down and Western blot. The fourth lane represents cell lysates directly used for western blot. β-actin was used as an internal control.


3). Wu H et al. Breaking the vicious loop between inflammation, oxidative stress and coagulation, a novel anti-thrombus insight of nattokinase by inhibiting LPS-induced inflammation and oxidative stress. Redox Biol 2020 Mar 11;32:101500 (PubMed: 32193146) [IF=9.986]

4). Zhang D et al. Cold to Hot: Rational Design of Minimalist Multifunctional Photo-Immunotherapy Nanoplatform toward Boosting Immunotherapy Capability. ACS Appl Mater Interfaces 2019 Aug 20 (PubMed: 31429272) [IF=8.758]

5). Wang Y et al. The interaction of YBX1 with G3BP1 promotes renal cell carcinoma cell metastasis via YBX1/G3BP1-SPP1- NF-κB signaling axis. J Exp Clin Cancer Res 2019 Sep 3;38(1):386 (PubMed: 31481087) [IF=7.068]

6). Li Y et al. 2',3'-Cyclic-nucleotide 3'-phosphodiesterase contributes to epithelial-mesenchymal transition of lens epithelial cells through the notch signalling pathway. Cell Prolif 2019 Nov;52(6):e12707 (PubMed: 31617266) [IF=5.753]

7). Liu H et al. Antimalarial drug pyrimethamine plays a dual role in anti-tumor proliferation and metastasis through targeting DHFR and TP. Mol Cancer Ther 2019 Jan 14 (PubMed: 30642883) [IF=5.615]

8). Xu Y et al. microRNA-690 regulates induced pluripotent stem cells (iPSCs) differentiation into insulin-producing cells by targeting Sox9. Stem Cell Res Ther 2019 Feb 15;10(1):59 (PubMed: 30767782) [IF=5.116]

9). Jiang Y et al. Hedgehog pathway inhibition causes primary follicle atresia and decreases female germline stem cell proliferation capacity or stemness. Stem Cell Res Ther 2019 Jul 5;10(1):198 (PubMed: 31277696) [IF=5.116]

10). Liu N;Sun Q;Xu H;Yu X;Chen W;Wei H;Jiang J;Xu Y;Lu W; et al. Hyperuricemia induces lipid disturbances mediated by LPCAT3 upregulation in the liver. FASEB J 2020 Aug 11. (PubMed: 32780898) [IF=4.966]

11). Liang G;Zhang H;Li Y;Pu M;Yang Y;Li C;Lu C;Xu P;Yu D; et al. Oryza sativa FER‐LIKE FE DEFICIENCY‐INDUCED TRANSCRIPTION FACTOR (OsFIT/OsbHLH156) interacts with OsIRO2 to regulate iron homeostasis. J Integr Plant Biol 2020 May;62(5):668-689. (PubMed: 32237201) [IF=4.885]

12). Ding S et al. Resveratrol alleviates chronic "real-world" ambient particulate matter-induced lung inflammation and fibrosis by inhibiting NLRP3 inflammasome activation in mice. Ecotoxicol Environ Saf 2019 Oct 30;182:109425 (PubMed: 31295660) [IF=4.872]

13). Meng X;Zhang J;Wu H;Yu D;Fang X; et al. Akkermansia muciniphila Aspartic Protease Amuc_1434* Inhibits Human Colorectal Cancer LS174T Cell Viability via TRAIL-Mediated Apoptosis Pathway. Int J Mol Sci 2020 May 11;21(9):E3385. (PubMed: 32403433) [IF=4.556]

14). Zhong L;Zhang H;Ding ZF;Li J;Lv JW;Pan ZJ;Xu DX;Yin ZS; et al. Erythropoietin-Induced Autophagy Protects Against Spinal Cord Injury and Improves Neurological Function via the Extracellular-Regulated Protein Kinase Signaling Pathway. Mol Neurobiol 2020 Jul 9. (PubMed: 32647973) [IF=4.500]

15). Chi Y et al. miR-516a-3p inhibits breast cancer cell growth and EMT by blocking the Pygo2/Wnt signalling pathway. J Cell Mol Med 2019 Sep;23(9):6295-6307 (PubMed: 31273950) [IF=4.486]

16). Xiao H;Chen H;Jiang R;Zhang L;Wang L;Gan H;Jiang N;Zhao J;Zhai X;Liang P; et al. NLRP6 contributes to inflammation and brain injury following intracerebral haemorrhage by activating autophagy. J Mol Med (Berl) 2020 Aug 12. (PubMed: 32783081) [IF=4.427]

17). Xie X et al. Advanced glycation end products reduce macrophage-mediated killing of Staphylococcus aureus by ARL8 upregulation and inhibition of autolysosome formation. Eur J Immunol 2020 Apr 6 (PubMed: 32250445) [IF=4.404]

18). Yuan CH et al. Secreted Rv1768 From RD14 of Mycobacterium tuberculosis Activates Macrophages and Induces a Strong IFN-γ-Releasing of CD4+ T Cells. Front Cell Infect Microbiol 2019 Oct 14;9:341 (PubMed: 31681622) [IF=4.123]

19). Tan L et al. Dihydroberberine, a hydrogenated derivative of berberine firstly identified in Phellodendri Chinese Cortex, exerts anti-inflammatory effect via dual modulation of NF-κB and MAPK signaling pathways. Int Immunopharmacol 2019 Aug 8;75:105802 (PubMed: 31401380) [IF=3.943]

20). An J et al. Preparation, characterization and application of anti-human OX40 ligand (OX40L) monoclonal antibodies and establishment of a sandwich ELISA for autoimmune diseases detection. Int Immunopharmacol 2018 Dec 15;67:260-267 (PubMed: 30562687) [IF=3.943]

21). Wei X;Sun C;Zhou RP;Ma GG;Yang Y;Lu C;Hu W; et al. Nerve growth factor promotes ASIC1a expression via the NF-κB pathway and enhances acid-induced chondrocyte apoptosis. Int Immunopharmacol 2020 Mar 5;82:106340. (PubMed: 32146316) [IF=3.943]

22). Li C;Liu J;Zhang Q;Cui K;Ge Q;Wang C;Chen Z; et al. Upregulation of E‑cadherin expression mediated by a novel dsRNA suppresses the growth and metastasis of bladder cancer cells by inhibiting β-catenin/TCF target genes. Int J Oncol 2018 Jun;52(6):1815-1826. (PubMed: 29620261) [IF=3.899]

23). Ding CL et al. Identification of retinoid acid induced 16 as a novel androgen receptor target in prostate cancer cells. Mol Cell Endocrinol 2020 Jan 31;506:110745 (PubMed: 32014455) [IF=3.871]

24). Shi Y et al. Synaptic Plasticity of Human Umbilical Cord Mesenchymal Stem Cell Differentiating into Neuron-like Cells In Vitro Induced by Edaravone. Stem Cells Int 2018 Oct 28;2018:5304279 (PubMed: 30510585) [IF=3.869]

25). Liu C et al. PADI4 stimulates esophageal squamous cell carcinoma tumor growth and up-regulates CA9 expression. Mol Carcinog 2018 Sep 22 (PubMed: 30242913) [IF=3.825]

26). Liu Y;Huang J;Yu N;Wei S;Liu Z; et al. Involvement of WNT2 in trophoblast cell behavior in preeclampsia development. Cell Cycle 2020 Aug 11;1-9. (PubMed: 32779546) [IF=3.699]

27). Lei X et al. The effect of fluid shear stress on fibroblasts and stem cells on plane and groove topographies. Cell Adh Migr 2020 Dec;14(1):12-23 (PubMed: 31942821) [IF=3.694]

28). Lu W et al. ApoE deficiency promotes non-alcoholic fatty liver disease in mice via impeding AMPK/mTOR mediated autophagy. Life Sci 2020 Apr 15;252:117601 (PubMed: 32304762) [IF=3.647]

29). Sun Y et al. CDC25A Facilitates Chemo-resistance in Ovarian Cancer Multicellular Spheroids by Promoting E-cadherin Expression and Arresting Cell Cycles. J Cancer 2019 Jun 2;10(13):2874-2884 (PubMed: 31281464) [IF=3.565]

30). Sun Y;Li S;Yu W;Chen C;Liu T;Li L;Zhang D;Zhao Z;Gao J;Wang X;Shi D;Liu L; et al. CD148 Serves as a Prognostic Marker of Gastric Cancer and Hinders Tumor Progression by Dephosphorylating EGFR. J Cancer 2020 Feb 19;11(9):2667-2678. (PubMed: 32201537) [IF=3.565]

31). Zhang T et al. Interaction with tumor‑associated macrophages promotes PRL‑3‑induced invasion of colorectal cancer cells via MAPK pathway‑induced EMT and NF‑κB signaling‑induced angiogenesis. Oncol Rep 2019 Mar 7 (PubMed: 30864736) [IF=3.417]

32). Sun X et al. (‑)‑Epigallocatechin‑3‑gallate inhibits bladder cancer stem cells via suppression of sonic hedgehog pathway. Oncol Rep 2019 Jul;42(1):425-435 (PubMed: 31180522) [IF=3.417]

33). He S et al. Triptolide inhibits PDGF-induced proliferation of ASMCs through G0/G1 cell cycle arrest and suppression of the AKT/NF-κB/cyclinD1 signaling pathway. Eur J Pharmacol 2019 Nov 19:172811 (PubMed: 31756335) [IF=3.263]

34). Chi Y et al. ZKSCAN3 promotes breast cancer cell proliferation, migration and invasion. Biochem Biophys Res Commun 2018 Sep 18;503(4):2583-2589 (PubMed: 30049438)

35). Guan SY et al. Anxiolytic effects of polydatin through the blockade of neuroinflammation in a chronic pain mouse model. Mol Pain 2020 Jan-Dec;16:1744806919900717 (PubMed: 31964240)

36). Wang Y et al. The Role of YB1 in Renal Cell Carcinoma Cell Adhesion. Int J Med Sci 2018 Aug 6;15(12):1304-1311 (PubMed: 30275756)

37). Ding S et al. Resveratrol reduces the inflammatory response in adipose tissue and improves adipose insulin signaling in high-fat diet-fed mice. PeerJ 2018 Jun 29;6:e5173 (PubMed: 29967759)

38). Li J et al. Rapamycin enhanced the antitumor effects of doxorubicin in myelogenous leukemia K562 cells by downregulating the mTOR/p70S6K pathway. Oncol Lett 2019 Sep;18(3):2694-2703 (PubMed: 31404320)

39). Luo L et al. Decreased expression of ubiquilin‑1 following neonatal hypoxia‑ischemic brain injury in mice. Mol Med Rep 2019 Apr 15 (PubMed: 31059032)

40). Wang W;Zheng S;He H;Ge H;Saeed BR; et al. N, N‑diethylaminobenzaldehyde targets aldehyde dehydrogenase to eradicate human pancreatic cancer cells. Exp Ther Med 2020 Jul;20(1):662-670. (PubMed: 32550888)

41). Qu Y et al. Yes-associated protein (YAP) predicts poor prognosis and regulates progression of esophageal squamous cell cancer through epithelial-mesenchymal transition. Exp Ther Med 2019 Oct;18(4):2993-3001 (PubMed: 31572541)

42). Fan L;Chen L;Liang Z;Bao H;Wang D;Dong Y;Zheng S;Xiao C;Du J;Li H; et al. A Polysaccharide Extract from Maitake Culinary-Medicinal Mushroom, Grifola frondosa (Agaricomycetes) Ameliorates Learning and Memory Function in Aluminum Chloride-Induced Amnesia in Mice. Int J Med Mushrooms 2019;21(11):1065-1074. (PubMed: 32450016)

43). Jin R;Xu J;Gao Q;Mao X;Yin J;Lu K;Guo Y;Zhang M;Cheng R; et al. IL-33-induced neutrophil extracellular traps degrade fibronectin in a murine model of bronchopulmonary dysplasia. Cell Death Discov 2020 May 4;6:33. (PubMed: 32377396)

44). et al. Effect of verapamil in the reversal of doxorubicin chemotherapy resistance in advanced gastric cancer.

45). et al. Thermosensitive hydrogels based on methylcellulose derivatives for prevention of postoperative adhesion.

46). Wang F;Zhang QB;Zhou Y;Liu AY;Huang PP;Liu Y; et al. Effect of ultrashort wave treatment on joint dysfunction and muscle atrophy in a rabbit model of extending knee joint contracture: Enhanced expression of myogenic differentiation. Knee 2020 Jun;27(3):795-802. (PubMed: 32201041)

47). et al. Effects of 2, 3′, 4, 4′, 5-pentachlorobiphenyl exposure during pregnancy on DNA methylation in the testis of offspring in the mouse.

48). et al. Enzyme-induced Fenton reaction coupling oxidation of o-phenylenediamine for sensitive and specific immunoassay.

49). et al. Study on the inhibitive effect of Catalpol on diabetic nephropathy.

50). et al. Alteration of the Wnt/GSK3β/β‑catenin signalling pathway by rapamycin ameliorates pathology in an Alzheimer's disease model.

51). Liding Zhang et al. Efficient Capture and Detection of Zika Virion by Polyclonal Antibody Against Prokaryotic Recombinant Envelope Protein. Jundishapur Journal of Microbiology 2018;

52). et al. Inhibitory effect of 17β‑estradiol on triglyceride synthesis in skeletal muscle cells is dependent on ESR1 and not ESR2.

53). Wang N et al. Neutrophil extracellular traps induced by VP1 contribute to pulmonary edema during EV71 infection. Cell Death Discov 2019 Jul 4;5:111 (PubMed: 31285854)

No comment
Total 0 records, divided into1 pages. First Prev Next Last

Submit Review

Support JPG, GIF, PNG format only
captcha
Catalog Number :

S0002-BP
(Blocking peptide available as S0002-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

IMPORTANT: For western blots, incubate membrane with diluted secondary antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 37°C, 2H.

To Top