Goat Anti-Mouse IgG (H+L) HRP
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$250 50ul
$350 100ul
$450 200ul

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  • Product Name
    Goat Anti-Mouse IgG (H+L) HRP
  • Catalog No.
  • RRID
  • Source
  • Application
  • Reactivity

Product Information


WB 1:3000~1:10000, IHC 1:200
*The optimal dilutions should be determined by the end user.








affinity purification.


Please cite this product as: Affinity Biosciences Cat# S0002, RRID:AB_2839430.

Storage Condition and Buffer:

HRP-labeled Goat IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl.

Immunogen Information in 3D


The antiserum was developed in goat using the mouse IgG as the immunogen.

Molecular Weight:

Observed Mol.Wt.: Not available.
Predicted Mol.Wt.: Not available.

Not available

Reference Citations:

1). Lei R et al. bHLH121 Functions as A Direct Link that Facilitates the Activation of FIT by bHLH IVc Transcription Factors for Maintaining Fe Homeostasis in Arabidopsis. Mol Plant 2020 Jan 18 (PubMed: 31962167) [IF=12.084]

2). Bian WX et al. Binding of cellular nucleolin with the viral core RNA G-quadruplex structure suppresses HCV replication. Nucleic Acids Res 2018 Nov 20 (PubMed: 30462330) [IF=11.501]

Application: WB    Species:human;    Sample:hepatoma cell lines

(B) G4 pull-down and Western blot. The fourth lane represents cell lysates directly used for western blot. β-actin was used as an internal control.

3). Wu H et al. Breaking the vicious loop between inflammation, oxidative stress and coagulation, a novel anti-thrombus insight of nattokinase by inhibiting LPS-induced inflammation and oxidative stress. Redox Biol 2020 Mar 11;32:101500 (PubMed: 32193146) [IF=9.986]

4). Fan H;Ding R;Liu W;Zhang X;Li R;Wei B;Su S;Jin F;Wei C;He X;Li X;Duan C; et al. Heat shock protein 22 modulates NRF1/TFAM-dependent mitochondrial biogenesis and DRP1-sparked mitochondrial apoptosis through AMPK-PGC1α signaling pathway to alleviate the early brain injury of subarachnoid hemorrhage in rats. Redox Biol 2021 Apr;40:101856. (PubMed: 33472123) [IF=9.986]

5). Zhang D et al. Cold to Hot: Rational Design of Minimalist Multifunctional Photo-Immunotherapy Nanoplatform toward Boosting Immunotherapy Capability. ACS Appl Mater Interfaces 2019 Aug 20 (PubMed: 31429272) [IF=8.758]

6). Wang Y et al. The interaction of YBX1 with G3BP1 promotes renal cell carcinoma cell metastasis via YBX1/G3BP1-SPP1- NF-κB signaling axis. J Exp Clin Cancer Res 2019 Sep 3;38(1):386 (PubMed: 31481087) [IF=7.068]

7). Zhou B;Fang L;Dong Y;Yang J;Chen X;Zhang N;Zhu Y;Huang T; et al. Mitochondrial quality control protects photoreceptors against oxidative stress in the H 2 O 2-induced models of retinal degeneration diseases. Cell Death Dis 2021 Apr 20;12(5):413. (PubMed: 33879768) [IF=6.304]

8). Li Y et al. 2',3'-Cyclic-nucleotide 3'-phosphodiesterase contributes to epithelial-mesenchymal transition of lens epithelial cells through the notch signalling pathway. Cell Prolif 2019 Nov;52(6):e12707 (PubMed: 31617266) [IF=5.753]

9). Liu H et al. Antimalarial drug pyrimethamine plays a dual role in anti-tumor proliferation and metastasis through targeting DHFR and TP. Mol Cancer Ther 2019 Jan 14 (PubMed: 30642883) [IF=5.615]

10). Ma S;Zhu L;Fan X;Luo T;Liu D;Liang Z;Hu X;Shi T;Tan W;Wang Z; et al. Melatonin derivatives combat with inflammation-related cancer by targeting the Main Culprit STAT3. Eur J Med Chem 2021 Feb 5;211:113027. (PubMed: 33248852) [IF=5.572]

11). Xu Y et al. microRNA-690 regulates induced pluripotent stem cells (iPSCs) differentiation into insulin-producing cells by targeting Sox9. Stem Cell Res Ther 2019 Feb 15;10(1):59 (PubMed: 30767782) [IF=5.116]

12). Jiang Y et al. Hedgehog pathway inhibition causes primary follicle atresia and decreases female germline stem cell proliferation capacity or stemness. Stem Cell Res Ther 2019 Jul 5;10(1):198 (PubMed: 31277696) [IF=5.116]

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18). Tao J;Yang P;Xie L;Pu Y;Guo J;Jiao J;Sun L;Lu D; et al. Gastrodin induces lysosomal biogenesis and autophagy to prevent the formation of foam cells via AMPK‐FoxO1‐TFEB signalling axis. J Cell Mol Med 2021 May 10. (PubMed: 33973365) [IF=4.658]

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23). Tao J;Yang P;Xie L;Pu Y;Guo J;Jiao J;Sun L;Lu D; et al. Gastrodin induces lysosomal biogenesis and autophagy to prevent the formation of foam cells via AMPK‐FoxO1‐TFEB signalling axis. J Cell Mol Med 2021 May 10. (PubMed: 33973365) [IF=4.486]

24). Song Z;Wu T;Sun J;Wang H;Hua F;Nicolas YSM;Kc R;Chen K;Jin Z;Liu J;Zhang M; et al. Metformin attenuates post‐epidural fibrosis by inhibiting the TGF‐β1/Smad3 and HMGB1/TLR4 signaling pathways. J Cell Mol Med 2021 Feb 21. (PubMed: 33611840) [IF=4.486]

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26). Xie X et al. Advanced glycation end products reduce macrophage-mediated killing of Staphylococcus aureus by ARL8 upregulation and inhibition of autolysosome formation. Eur J Immunol 2020 Apr 6 (PubMed: 32250445) [IF=4.404]

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28). Yuan CH et al. Secreted Rv1768 From RD14 of Mycobacterium tuberculosis Activates Macrophages and Induces a Strong IFN-γ-Releasing of CD4+ T Cells. Front Cell Infect Microbiol 2019 Oct 14;9:341 (PubMed: 31681622) [IF=4.123]

29). Tan L et al. Dihydroberberine, a hydrogenated derivative of berberine firstly identified in Phellodendri Chinese Cortex, exerts anti-inflammatory effect via dual modulation of NF-κB and MAPK signaling pathways. Int Immunopharmacol 2019 Aug 8;75:105802 (PubMed: 31401380) [IF=3.943]

30). Wei X;Sun C;Zhou RP;Ma GG;Yang Y;Lu C;Hu W; et al. Nerve growth factor promotes ASIC1a expression via the NF-κB pathway and enhances acid-induced chondrocyte apoptosis. Int Immunopharmacol 2020 Mar 5;82:106340. (PubMed: 32146316) [IF=3.943]

31). An J et al. Preparation, characterization and application of anti-human OX40 ligand (OX40L) monoclonal antibodies and establishment of a sandwich ELISA for autoimmune diseases detection. Int Immunopharmacol 2018 Dec 15;67:260-267 (PubMed: 30562687) [IF=3.943]

32). Wang X;Yin H;Fan L;Zhou Y;Tang X;Fei X;Tang H;Peng J;Zhang J;Xue Y;Luo J;Jin Q;Jin Q; et al. Shionone alleviates NLRP3 inflammasome mediated pyroptosis in interstitial cystitis injury. Int Immunopharmacol 2020 Nov 19;107132. (PubMed: 33223465) [IF=3.943]

33). Khayeka-Wandabwa C;Ma X;Jia Y;Bureik M; et al. Concomitant occurence of multiple autoantibodies against human cytochromes P450. Int Immunopharmacol 2021 Aug 28;100:108087. (PubMed: 34464888) [IF=3.943]

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35). Ding CL et al. Identification of retinoid acid induced 16 as a novel androgen receptor target in prostate cancer cells. Mol Cell Endocrinol 2020 Jan 31;506:110745 (PubMed: 32014455) [IF=3.871]

36). Shi Y et al. Synaptic Plasticity of Human Umbilical Cord Mesenchymal Stem Cell Differentiating into Neuron-like Cells In Vitro Induced by Edaravone. Stem Cells Int 2018 Oct 28;2018:5304279 (PubMed: 30510585) [IF=3.869]

37). Liu C et al. PADI4 stimulates esophageal squamous cell carcinoma tumor growth and up-regulates CA9 expression. Mol Carcinog 2018 Sep 22 (PubMed: 30242913) [IF=3.825]

38). Liu Y;Huang J;Yu N;Wei S;Liu Z; et al. Involvement of WNT2 in trophoblast cell behavior in preeclampsia development. Cell Cycle 2020 Aug 11;1-9. (PubMed: 32779546) [IF=3.699]

39). Lei X et al. The effect of fluid shear stress on fibroblasts and stem cells on plane and groove topographies. Cell Adh Migr 2020 Dec;14(1):12-23 (PubMed: 31942821) [IF=3.694]

40). Zhang Y;Gu Y;Chen Y;Huang Z;Li M;Jiang W;Chen J;Rao W;Luo S;Chen Y;Chen J;Li L;Jia Y;Liu M;Zhou F; et al. Dingxin Recipe IV attenuates atherosclerosis by regulating lipid metabolism through LXR-α/SREBP1 pathway and modulating the gut microbiota in ApoE-/- mice fed with HFD. J Ethnopharmacol 2020 Oct 1;266:113436. (PubMed: 33011372) [IF=3.690]

41). Lu W et al. ApoE deficiency promotes non-alcoholic fatty liver disease in mice via impeding AMPK/mTOR mediated autophagy. Life Sci 2020 Apr 15;252:117601 (PubMed: 32304762) [IF=3.647]

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45). An Q;Liu T;Wang MY;Yang YJ;Zhang ZD;Liu ZJ;Yang B; et al. KRT7 promotes epithelial‑mesenchymal transition in ovarian cancer via the TGF‑β/Smad2/3 signaling pathway. Oncol Rep 2020 Dec 8;45(2):481-492. (PubMed: 33416175) [IF=3.417]

46). Sun X et al. (‑)‑Epigallocatechin‑3‑gallate inhibits bladder cancer stem cells via suppression of sonic hedgehog pathway. Oncol Rep 2019 Jul;42(1):425-435 (PubMed: 31180522) [IF=3.417]

47). Zhang T et al. Interaction with tumor‑associated macrophages promotes PRL‑3‑induced invasion of colorectal cancer cells via MAPK pathway‑induced EMT and NF‑κB signaling‑induced angiogenesis. Oncol Rep 2019 Mar 7 (PubMed: 30864736) [IF=3.417]

48). Fang Q;Zheng B;Liu N;Liu J;Liu W;Huang X;Zeng X;Chen L;Li Z;Ouyang D; et al. Trimethylamine N-Oxide Exacerbates Renal Inflammation and Fibrosis in Rats With Diabetic Kidney Disease. Front Physiol 2021 Jun 16;12:682482. (PubMed: 34220546) [IF=3.367]

49). Chen S;Zhou L;Sun J;Qu Y;Chen M; et al. The Role of cAMP-PKA Pathway in Lactate-Induced Intramuscular Triglyceride Accumulation and Mitochondria Content Increase in Mice. Front Physiol 2021 Sep 13;12:709135. (PubMed: 34588991) [IF=3.367]

50). He J;Mao Y;Huang W;Li M;Zhang H;Qing Y;Lu S;Xiao H;Li K; et al. Methylcrotonoyl-CoA Carboxylase 2 Promotes Proliferation, Migration and Invasion and Inhibits Apoptosis of Prostate Cancer Cells Through Regulating GLUD1-P38 MAPK Signaling Pathway. Onco Targets Ther 2020 Jul 28;13:7317-7327. (PubMed: 32801758) [IF=3.337]

51). He S et al. Triptolide inhibits PDGF-induced proliferation of ASMCs through G0/G1 cell cycle arrest and suppression of the AKT/NF-κB/cyclinD1 signaling pathway. Eur J Pharmacol 2019 Nov 19:172811 (PubMed: 31756335) [IF=3.263]

52). Wang X;Li Y;Fan GF;Zhang TY;Sun B;Fan PS; et al. Effect of verapamil in the reversal of doxorubicin chemotherapy resistance in advanced gastric cancer. Eur Rev Med Pharmacol Sci 2020 Jul;24(14):7753-7763. (PubMed: 32744702) [IF=3.024]

53). Chi Y et al. ZKSCAN3 promotes breast cancer cell proliferation, migration and invasion. Biochem Biophys Res Commun 2018 Sep 18;503(4):2583-2589 (PubMed: 30049438)

54). Guan SY et al. Anxiolytic effects of polydatin through the blockade of neuroinflammation in a chronic pain mouse model. Mol Pain 2020 Jan-Dec;16:1744806919900717 (PubMed: 31964240)

55). Liang J;Li W;Zhuang N;Wen S;Huang S;Lu W;Zhou Y;Liao G;Zhang B;Liu C; et al. Experimental study on bone defect repair by BMSCs combined with a light-sensitive material: gC3N4/rGO. J Biomater Sci Polym Ed 2020 Oct 8;1-18. (PubMed: 32975477)

56). Wang Y et al. The Role of YB1 in Renal Cell Carcinoma Cell Adhesion. Int J Med Sci 2018 Aug 6;15(12):1304-1311 (PubMed: 30275756)

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Catalog Number :

(Blocking peptide available as S0002-BP)

Price/Size :

Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

IMPORTANT: For western blots, incubate membrane with diluted secondary antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 37°C, 2H.

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