Goat Anti-Rabbit IgG (H+L) HRP
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$250 50ul
$350 100ul
$450 200ul

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  • Product Name
    Goat Anti-Rabbit IgG (H+L) HRP
  • Catalog No.
    S0001
  • Source
    Goat
  • Application
    WB,IHC
  • Reactivity
    Rabbit

Product Information

Applications:

WB 1:3000~1:10000, IHC 1:200

Reactivity:

Rabbit

Source:

Goat

Clonality:

Polyclonal

Purification:

affinity purification.

Storage Condition and Buffer:

HRP-labeled Goat IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl.

Immunogen Information

Immunogen:

The antiserum was developed in goat using the Rabbit IgG as the immunogen.

Molecular Weight:

Observed Mol.Wt.: Not available.
Predicted Mol.Wt.: Not available.

Not available

Reference Citations:

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13). Xiong Y et al. Hypoxia-inducible factor 1α-induced epithelial-mesenchymal transition of endometrial epithelial cells may contribute to the development of endometriosis. Hum Reprod 2016 Jun;31(6):1327-38 (PubMed: 27094478) [IF=5.506]

Application: WB    Species:human;    Sample:Not available

Figure 2 The expression of epithelial –mesenchymal transition (EMT) markers and b-catenin was detected at each time point. (A) Immunoblotting analysis of human primary cultured endometrial epithelial cell extracts using the corresponding antibodies. The ratios of each protein relative to non-treated cells were normalized to GAPDH. (B) The relative expression of HIF-1a, N-cadherin, E-cadherin, b-catenin, vimentin and snail proteins in human endometrial epithelial glands under hypoxic conditions at each time point was investigated by western blot. Data are represented as mean+SD and are representative of the relative expression of protein normalized by GAPDH. All experiments were repeated four times. Data were evaluated by one-way ANOVA analysis (*P , 0.05, **P , 0.01 compared with untreated group). (C) The changed cellular morphologies of human endometrial epithelial glands in hypoxia compared with cells in normoxia, the hypoxic time was 48 h. Red arrows indicate the spindle-shaped and fibroblast-like cells.


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93). Li H et al. Dysifragilone A inhibits LPS‑induced RAW264.7 macrophage activation by blocking the p38 MAPK signaling pathway. Mol Med Rep 2018 Jan;17(1):674-682 (PubMed: 29115475)

94). Li H et al. Dysifragilone A inhibits LPS‑induced RAW264.7 macrophage activation by blocking the p38 MAPK signaling pathway. Mol Med Rep 2018 Jan;17(1):674-682 (PubMed: 29115475)

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Application: WB    Species:mouse;    Sample:Not available


96). Luo L et al. Decreased expression of ubiquilin‑1 following neonatal hypoxia‑ischemic brain injury in mice. Mol Med Rep 2019 Apr 15 (PubMed: 31059032)

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105). Xiaojun Li et al. Recombinant human irisin regulated collagen II, matrix metalloproteinase‑13 and the Wnt/β‑catenin and NF‑κB signaling pathways in interleukin‑1β‑induced human SW1353 cells. EXP THER MED 2020 Feb 26

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109). Qian SQ et al. Vacuum therapy prevents corporeal veno-occlusive dysfunction and penile shrinkage in a cavernosal nerve injured rat model. Asian J Androl 2019 Jun 25 (PubMed: 31249269)

110). Liu H et al. Long non-coding RNA MALAT1 mediates hypoxia-induced pro-survival autophagy of endometrial stromal cells in endometriosis. J Cell Mol Med 2019 Jan;23(1):439-452 (PubMed: 30324652)

111). Xiangli Yan et al. Calycosin-7-O-β-D-glucoside Attenuates OGD/R-Induced Damage by Preventing Oxidative Stress and Neuronal Apoptosis via the SIRT1/FOXO1/PGC-1α Pathway in HT22 Cells. NEURAL PLAST 2019, Article ID 8798069, 11 pages

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117). et al. Inhibitory effect of 17β‑estradiol on triglyceride synthesis in skeletal muscle cells is dependent on ESR1 and not ESR2.

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Catalog Number :

S0001-BP
(Blocking peptide available as S0001-BP)

Price/Size :

$200/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

IMPORTANT: For western blots, incubate membrane with diluted secondary antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 37°C, 2H.

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