Goat Anti-Rabbit IgG (H+L) HRP
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$250 50ul
$350 100ul
$450 200ul

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  • Product Name
    Goat Anti-Rabbit IgG (H+L) HRP
  • Catalog No.
    S0001
  • Source
    Goat
  • Application
    WB,IHC
  • Reactivity:
    Rabbit

Product Information

Applications:

WB 1:3000~1:10000, IHC 1:200

Reactivity:

Rabbit

Source:

Goat

Clonality:

Polyclonal

Purification:

affinity purification.

Storage Condition and Buffer:

HRP-labeled Goat IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl.

Immunogen Information

Immunogen:

The antiserum was developed in goat using the Rabbit IgG as the immunogen.

Molecular Weight:

Observed Mol.Wt.: Not available.
Predicted Mol.Wt.: Not available.

Not available

Reference Citations:

1). Bian WX et al. Binding of cellular nucleolin with the viral core RNA G-quadruplex structure suppresses HCV replication. Nucleic Acids Res 2018 Nov 20 (PubMed: 30462330) [IF=11.561]

2). Xu X et al. Progerin accumulation in nucleus pulposus cells impairs mitochondrial function and induces intervertebral disc degeneration and therapeutic effects of sulforaphane. Theranostics 2019 Apr 12;9(8):2252-2267 (PubMed: 31149042) [IF=8.537]

3). et al. Inhibitory effect of 17β‑estradiol on triglyceride synthesis in skeletal muscle cells is dependent on ESR1 and not ESR2.

4). et al. Synergistic protection of matrine and lycopene against lipopolysaccharide‑induced acute lung injury in mice.

5). Jin X et al. Tiliroside, the major component of Agrimonia pilosa Ledeb ethanol extract, inhibits MAPK/JNK/p38-mediated inflammation in lipopolysaccharide-activated RAW 264.7 macrophages. Exp Ther Med 2016 Jul;12(1):499-505 (PubMed: 27347085)

6). Yang Y et al. Black Sesame Seeds Ethanol Extract Ameliorates Hepatic Lipid Accumulation, Oxidative Stress and Insulin Resistance in Fructose-induced Nonalcoholic Fatty Liver Disease. J Agric Food Chem 2018 Sep 24 (PubMed: 30244573)

7). Yuan Q et al. The effect of CA125 on metastasis of ovarian cancer: old marker new function. Oncotarget 2017 Jul 25;8(30):50015-50022 (PubMed: 28637006)

8). Luo ML et al. Conditioned Medium from Human Umbilical Vein Endothelial Cells Promotes Proliferation, Migration, Invasion and Angiogenesis of Adipose Derived Stem Cells. Curr Med Sci 2018 Feb;38(1):124-130 (PubMed: 30074161)

9). Han Y et al. Antiangiogenic effects of catalpol on rat corneal neovascularization. Mol Med Rep 2018 Feb;17(2):2187-2194 (PubMed: 29207076)

10). Xin X et al. The suppressive role of calcium sensing receptor in endometrial cancer. Sci Rep 2018 Jan 18;8(1):1076 (PubMed: 29348629)

11). Yun-Peng Shi et al. Synaptic plasticity of Human Umbilical Cord Mesenchymal Stem Cell differentiating into neuron like cells in vitro induced by Edavarone. STEM CELLS INT 2018 Aug;

12). Xin Y et al. Versatile Pt NCs-based chemotherapeutic agents significantly induce the apoptosis of cisplatin-resistant non-small cell lung cancer. Biochem Biophys Res Commun 2019 Mar 15 (PubMed: 30885437)

13). Cui X et al. IL22 furthers malignant transformation of rat mesenchymal stem cells, possibly in association with IL22RA1/STAT3 signaling. Oncol Rep 2019 Apr;41(4):2148-2158 (PubMed: 30816520)

14). Liu Y et al. (3R, 7R)-7-Acetoxyl-9-Oxo-de-O-Methyllasiodiplodin, a Secondary Metabolite of Penicillium Sp., Inhibits LPS-Mediated Inflammation in RAW 264.7 Macrophages through Blocking ERK/MAPKs and NF-κB Signaling Pathways. Inflammation 2019 Apr 23 (PubMed: 31011928)

15). et al. (‑)‑Epigallocatechin‑3‑gallate inhibits bladder cancer stem cells via suppression of sonic hedgehog pathway.

16). Pan Q et al. A single-stranded DNA aptamer against mannose-capped lipoarabinomannan enhances anti-tuberculosis activity of macrophages through downregulation of lipid-sensing nuclear receptor peroxisome proliferator-activated receptor γ expression. Microbiol Immunol 2017 Feb;61(2):92-102 (PubMed: 28206680)

17). Zhao ZH et al. PirB Overexpression Exacerbates Neuronal Apoptosis by Inhibiting TrkB and mTOR Phosphorylation After Oxygen and Glucose Deprivation Injury. Cell Mol Neurobiol 2017 May;37(4):707-715 (PubMed: 27443384)

18). Zhong W et al. Long non-coding RNA MIAT promotes epithelial-mesenchymal transition and is an independent risk factor for poor prognosis of tongue squamous cell carcinoma. J Oral Pathol Med 2019 Jun 5 (PubMed: 31166624)

19). Seo E et al. Exendin-4 in combination with adipose-derived stem cells promotes angiogenesis and improves diabetic wound healing. J Transl Med 2017 Feb 15;15(1):35 (PubMed: 28202074)

20). Tao M et al. α-Mangostin Alleviated Lipopolysaccharide Induced Acute Lung Injury in Rats by Suppressing NAMPT/NAD Controlled Inflammatory Reactions. Evid Based Complement Alternat Med 2018 Oct 10;2018:5470187 (PubMed: 30405740)

21). Zeng SY et al. Nox1/4 dual inhibitor GKT137831 attenuates hypertensive cardiac remodelling associating with the inhibition of ADAM17-dependent proinflammatory cytokines-induced signalling pathways in the rats with abdominal artery constriction. Biomed Pharmacother 2019 Jan;109:1907-1914 (PubMed: 30551445)

22). Shen M et al. Xanthatin inhibits corneal neovascularization by inhibiting the VEGFR2‑mediated STAT3/PI3K/Akt signaling pathway. Int J Mol Med 2018 Aug;42(2):769-778 (PubMed: 29717775)

23). Ma MQ et al. A 6 hour therapeutic window, optimal for interventions targeting AMPK synergism and apoptosis antagonism, for cardioprotection against myocardial ischemic injury: an experimental study on rats. Am J Cardiovasc Dis 2015 Mar 20;5(1):63-71 (PubMed: 26064793)

24). Ding S et al. Resveratrol reduces the inflammatory response in adipose tissue and improves adipose insulin signaling in high-fat diet-fed mice. PeerJ 2018 Jun 29;6:e5173 (PubMed: 29967759)

25). Ding S et al. Combined effects of ambient particulate matter exposure and a high-fat diet on oxidative stress and steatohepatitis in mice. PLoS One 2019 Mar 28;14(3):e0214680 (PubMed: 30921449)

26). WenjingZong et al. Ginsenoside compound K attenuates cognitive deficits in vascular dementia rats by reducing the Ab deposition. Journal of Pharmacological Sciences 2019

27). Tao H et al. LncRNA MEG3 inhibits trophoblast invasion and trophoblast-mediated VSMC loss in uterine spiral artery remodeling. Mol Reprod Dev 2019 May 8 (PubMed: 31066488)

28). Li H et al. MicroRNA-181a regulates epithelial-mesenchymal transition by targeting PTEN in drug-resistant lung adenocarcinoma cells. Int J Oncol 2015 Oct;47(4):1379-92 (PubMed: 26323677)

29). et al. Strontium promotes osteogenic differentiation by activating autophagy via the the AMPK/mTOR signaling pathway in MC3T3‑E1 cells.

30). Guo L et al. Resveratrol Induces Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Neuron-Like Cells. Stem Cells Int 2017;2017:1651325 (PubMed: 28512471)

31). Zhang Q et al. Anti-inflammatory action of ambuic acid, a natural product isolated from the solid culture of Pestalotiopsis neglecta, through blocking ERK/JNK mitogen-activated protein kinase signaling pathway. Exp Ther Med 2018 Aug;16(2):1538-1546 (PubMed: 30116402)

32). Li H et al. Dysifragilone A inhibits LPS‑induced RAW264.7 macrophage activation by blocking the p38 MAPK signaling pathway. Mol Med Rep 2018 Jan;17(1):674-682 (PubMed: 29115475)

33). Zhao YX et al. Fine Particulate Matter-Induced Exacerbation of Allergic Asthma via Activation of T-cell Immunoglobulin and Mucin Domain 1. Chin Med J (Engl) 2018 Oct 20;131(20):2461-2473 (PubMed: 30334531)

34). Li H et al. Dysifragilone A inhibits LPS‑induced RAW264.7 macrophage activation by blocking the p38 MAPK signaling pathway. Mol Med Rep 2018 Jan;17(1):674-682 (PubMed: 29115475)

35). Han X et al. The relationship between phospho-p38, MMP-9, and MICA expression in pituitary adenomas demonstrates a new mechanism of pituitary adenoma immune escape. World Neurosurg 2018 Nov 17 (PubMed: 30458325)

36). Yong Wang et al. The Role of YB1 in Renal Cell Carcinoma Cell Adhesion. Int J Med Sci 2018; 15(12):1304-1311

37). Zeng SY et al. A Reduction in ADAM17 Expression Is Involved in the Protective Effect of the PPAR-α Activator Fenofibrate on Pressure Overload-Induced Cardiac Hypertrophy. PPAR Res 2018 Jul 19;2018:7916953 (PubMed: 30105051)

38). Zhang T et al. Interaction with tumor‑associated macrophages promotes PRL‑3‑induced invasion of colorectal cancer cells via MAPK pathway‑induced EMT and NF‑κB signaling‑induced angiogenesis. Oncol Rep 2019 Mar 7 (PubMed: 30864736)

39). Luo DD et al. Tetrahydrocurcumin and octahydrocurcumin, the primary and final hydrogenated metabolites of curcumin, possess superior hepatic-protective effect against acetaminophen-induced liver injury: Role of CYP2E1 and Keap1-Nrf2 pathway. Food Chem Toxicol 2019 Jan;123:349-362 (PubMed: 30423402)

40). Luo L et al. Decreased expression of ubiquilin‑1 following neonatal hypoxia‑ischemic brain injury in mice. Mol Med Rep 2019 Apr 15 (PubMed: 31059032)

41). et al. Alteration of the Wnt/GSK3β/β‑catenin signalling pathway by rapamycin ameliorates pathology in an Alzheimer's disease model.

42). Xiong Y et al. Hypoxia-inducible factor 1α-induced epithelial-mesenchymal transition of endometrial epithelial cells may contribute to the development of endometriosis. Hum Reprod 2016 Jun;31(6):1327-38 (PubMed: 27094478)

Application: WB    Species:human;    Sample:Not available

Figure 2 The expression of epithelial –mesenchymal transition (EMT) markers and b-catenin was detected at each time point. (A) Immunoblotting analysis of human primary cultured endometrial epithelial cell extracts using the corresponding antibodies. The ratios of each protein relative to non-treated cells were normalized to GAPDH. (B) The relative expression of HIF-1a, N-cadherin, E-cadherin, b-catenin, vimentin and snail proteins in human endometrial epithelial glands under hypoxic conditions at each time point was investigated by western blot. Data are represented as mean+SD and are representative of the relative expression of protein normalized by GAPDH. All experiments were repeated four times. Data were evaluated by one-way ANOVA analysis (*P , 0.05, **P , 0.01 compared with untreated group). (C) The changed cellular morphologies of human endometrial epithelial glands in hypoxia compared with cells in normoxia, the hypoxic time was 48 h. Red arrows indicate the spindle-shaped and fibroblast-like cells.


43). Liu C et al. PADI4 stimulates esophageal squamous cell carcinoma tumor growth and up-regulates CA9 expression. Mol Carcinog 2018 Sep 22 (PubMed: 30242913)

44). Bao H et al. Lentinan produces a robust antidepressant-like effect via enhancing the prefrontal Dectin-1/AMPA receptor signaling pathway. Behav Brain Res 2017 Jan 15;317:263-271 (PubMed: 27693847)

45). Chuanchang Li et al. Upregulation of E‑cadherin expression mediated by a novel dsRNA suppresses the growth and metastasis of bladder cancer cells by inhibiting β-catenin/TCF target genes. Int J Oncol 2018 Mar;1815-1826

46). Liu H et al. Long non-coding RNA MALAT1 mediates hypoxia-induced pro-survival autophagy of endometrial stromal cells in endometriosis. J Cell Mol Med 2018 Oct 15 (PubMed: 30324652)

47). Ge QD et al. Differential Expression of miRNAs in the Hippocampi of Offspring Rats Exposed to Fluorine Combined with Aluminum during the Embryonic Stage and into Adulthood. Biol Trace Elem Res 2018 Jul 22 (PubMed: 30033483)

48). Chi S et al. Knockdown of long non-coding HOTAIR enhances the sensitivity to progesterone in endometrial cancer by epigenetic regulation of progesterone receptor isoform B. Cancer Chemother Pharmacol 2018 Nov 15 (PubMed: 30443761)

49). Cui X et al. IL22 furthers malignant transformation of rat mesenchymal stem cells, possibly in association with IL22RA1/STAT3 signaling. Oncol Rep 2019 Apr;41(4):2148-2158 (PubMed: 30816520)

50). An X et al. Increasing the TRPM2 Channel Expression in Human Neuroblastoma SH-SY5Y Cells Augments the Susceptibility to ROS-Induced Cell Death. Cells 2019 Jan 8;8(1) (PubMed: 30625984)

51). Keren Wu et al. Antitumor effect of ginsenoside Rg3 on gallbladder cancer by inducing endoplasmic reticulum stress‑mediated apoptosis in vitro and in vivo. ONCOL LETT 2018 Aug;0:0-0

52). Zeng SY et al. Rutaecarpine prevents hypertensive cardiac hypertrophy involving the inhibition of Nox4-ROS-ADAM17 pathway. J Cell Mol Med 2019 Apr 5 (PubMed: 30953402)

53). Chen Z et al. Lower Expression of Gelsolin in Colon Cancer and Its Diagnostic Value in Colon Cancer Patients. J Cancer 2019 Jan 30;10(5):1288-1296 (PubMed: 30854138)

54). et al. Low dose Bisphenol A (BPA) induced DNA damage and tumorigenic events in MCF-10A cells.

55). Nan C et al. Tetramethylpyrazine induces differentiation of human umbilical cord-derived mesenchymal stem cells into neuron-like cells in vitro. Int J Oncol 2016 Jun;48(6):2287-94 (PubMed: 27035275)

56). Jin X et al. 11-O-acetylcyathatriol inhibits MAPK/p38-mediated inflammation in LPS-activated RAW 264.7 macrophages and has a protective effect on ethanol-induced gastric injury. Mol Med Rep 2016 Jul;14(1):874-80 (PubMed: 27222252)

Application: WB    Species:mouse;    Sample:Not available


57). Li CL et al. Comparison of anti-inflammatory effects of berberine, and its natural oxidative and reduced derivatives from Rhizoma Coptidis in vitro and in vivo. Phytomedicine 2019 Jan;52:272-283 (PubMed: 30599908)

58). Shao Q et al. MicroRNA-139-5p affects cisplatin sensitivity in human nasopharyngeal carcinoma cells by regulating the epithelial-to-mesenchymal transition. Gene 2018 Apr 30;652:48-58 (PubMed: 29427737)

59). Huang S et al. Nepeta angustifolia attenuates responses to vascular inflammation in high glucose-induced human umbilical vein endothelial cells through heme oxygenase-1 induction. J Ethnopharmacol 2019 Mar 1;231:187-196 (PubMed: 30419276)

60). Zeng SY et al. Evidence That ADAM17 Mediates the Protective Action of CGRP against Angiotensin II-Induced Inflammation in Vascular Smooth Muscle Cells. Mediators Inflamm 2018 Jun 12;2018:2109352 (PubMed: 30046277)

61). Shen YH et al. Ethyl Rosmarinate Protects High Glucose-Induced Injury in Human Endothelial Cells. Molecules 2018 Dec 19;23(12) (PubMed: 30572638)

62). Li A et al. Epigallocatechin-3-gallate affects the proliferation, apoptosis, migration and invasion of tongue squamous cell carcinoma through the hippo-TAZ signaling pathway. Int J Mol Med 2018 Aug 9 (PubMed: 30106116)

63). Fan H et al. The in vitro and in vivo anti-inflammatory effect of osthole, the major natural coumarin from Cnidium monnieri (L.) Cuss, via the blocking of the activation of the NF-κB and MAPK/p38 pathways. Phytomedicine 2019 Feb 18;58:152864 (PubMed: 30878874)

64). Liu X et al. The role of Delta-like 4 ligand/Notch-ephrin-B2 cascade in the pathogenesis of preeclampsia by regulating functions of endothelial progenitor cell. Placenta 2015 Sep;36(9):1002-10 (PubMed: 26212082)

65). Jia D et al. A Novel Berberine - Metformin Hybrid Compound Exerts Therapeutic Effects on Obese Type 2 Diabetic Rats. Clin Exp Pharmacol Physiol 2019 Mar 18 (PubMed: 30883863)

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Catalog Number :

S0001-BP

Price/Size :

$200/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

IMPORTANT: For western blots, incubate membrane with diluted secondary antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 37°C, 2H.