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  • Product Name
    Tubulin alpha Antibody
  • Catalog No.
  • RRID
  • Source
  • Application
  • Reactivity
    Human, Mouse, Zebrafish, Rabbit
  • UniProt
  • Mol.Wt
  • Concentration
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Product Information

Alternative Names:Expand▼

TUBA4A, Alpha-tubulin 1, TUBA1, Testis-specific alpha-tubulin, Tubulin, alpha 4a, H2-ALPHA, Tubulin alpha-1 chain, Tubulin alpha-4 chain, Tubulin alpha-4A chain, Tubulin H2-alpha, Tubulin, alpha 1


WB: 1:3000-1:10000
*The optimal dilutions should be determined by the end user.


Human, Mouse, Zebrafish, Rabbit





Clone Number:



affinity purification.


The α-tubulin antibody can detects ebrafish endogenousα-tubulin protein.


Please cite this product as: Affinity Biosciences Cat# T0033, RRID:AB_2839424.





Storage Condition and Buffer:

PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C. Do not aliquot the antibody.

Immunogen Information in 3D


Peptide from Zebrafish alpha tubulin.


>>Visit The Human Protein Atlas

Gene ID:

Gene Name:


Molecular Weight:

Observed Mol.Wt.: 55kD.
Predicted Mol.Wt.: 50kDa(Calculated)..

Subcellular Location:

Cytoplasm > cytoskeleton.


Tubulin is one of several members of a small family of globular proteins. The tubulin superfamily includes five distinct families, the alpha-, beta-, gamma-, delta-, and epsilon-tubulins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 KD. Microtubules are assembled from dimers of α- and β-tubulin.


Research Background


Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Post-translational Modifications:

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold.

Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).

Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly.

Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability.

Subcellular Location:


Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells. Interacts with CFAP157 (By similarity).


Belongs to the tubulin family.

Research Fields

Research Fields:

· Cellular Processes > Transport and catabolism > Phagosome.(View pathway)
· Cellular Processes > Cell growth and death > Apoptosis.(View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction.(View pathway)
· Cellular Processes > Cellular community - eukaryotes > Gap junction.(View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

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Catalog Number :

(Blocking peptide available as T0033-BP)

Price/Size :

Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

P68366 as Substrate
Site PTM Type Enzyme
S6 Phosphorylation
Y24 Phosphorylation
S38 Phosphorylation
K40 Acetylation
T41 Phosphorylation
S48 Phosphorylation
C54 S-Nitrosylation
K60 Ubiquitination
T73 Phosphorylation
T94 Phosphorylation
K96 Acetylation
K96 Sumoylation
K96 Ubiquitination
Y103 Phosphorylation
Y108 Phosphorylation
T109 Phosphorylation
K112 Acetylation
K112 Sumoylation
K112 Ubiquitination
K124 Methylation
K124 Ubiquitination
S158 Phosphorylation
Y161 Phosphorylation
K163 Acetylation
K163 Methylation
K163 Ubiquitination
K164 Ubiquitination
K166 Ubiquitination
Y172 Phosphorylation
Y185 Phosphorylation
S187 Phosphorylation
T190 Phosphorylation
T191 Phosphorylation
T193 Phosphorylation
T194 Phosphorylation
S198 Phosphorylation
Y210 Phosphorylation
T223 Phosphorylation
Y224 Phosphorylation
T225 Phosphorylation
S232 Phosphorylation
S236 Phosphorylation
S237 Phosphorylation
T239 Phosphorylation
S241 Phosphorylation
T253 Phosphorylation
T257 Phosphorylation
Y262 Phosphorylation
T271 Phosphorylation
Y272 Phosphorylation
S277 Phosphorylation
K280 Acetylation
K280 Ubiquitination
Y282 Phosphorylation
S287 Phosphorylation
T292 Phosphorylation
C295 S-Nitrosylation
K304 Ubiquitination
K311 Acetylation
K311 Ubiquitination
Y312 Phosphorylation
Y319 Phosphorylation
K326 Ubiquitination
K336 Sumoylation
K336 Ubiquitination
K338 Ubiquitination
R339 Methylation
S340 Phosphorylation
C347 S-Nitrosylation
T349 Phosphorylation
K352 Acetylation
K352 Sumoylation
K352 Ubiquitination
Y357 Phosphorylation
T361 Phosphorylation
K370 Acetylation
K370 Sumoylation
K370 Ubiquitination
C376 S-Nitrosylation
S379 Phosphorylation
K394 Acetylation
K394 Sumoylation
K394 Ubiquitination
Y399 Phosphorylation
K401 Acetylation
K401 Sumoylation
K401 Ubiquitination
Y408 Phosphorylation
S419 Phosphorylation
Y432 Phosphorylation P43405 (SYK)
S439 Phosphorylation
Y440 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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