GAPDH Antibody - AF7021

Price Size
$150 50ul
$250 100ul
$350 200ul
$1200 1ml

Same day delivery

For pricing and ordering contact:

local distributors
  • Product Name
    GAPDH Antibody
  • Catalog No.
    AF7021
  • RRID
    AB_2839421
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC,ELISA(peptide)
  • Reactivity
    Human, Mouse, Rat, Pig, Bovine, Goat, Monkey, Chicken
  • Prediction
    Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
  • UniProt
  • Mol.Wt
    37kD;
    36kDa(Calculated).
  • Concentration
    1mg/ml

  • Browse similar products>>

Related Products

 #AF7021-BP GAPDH Blocking Peptide $350/1mg

Product Information

Alternative Names:Expand▼

GAPDH, A1 40 kd subunit, Activator 1 40 kd subunit, G3PD, GAPD, G3pdh, Rfc40, Rf-c 40 kd subunit

Applications:

WB 1:3000-1:30000, IHC 1:200, IF/ICC 1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat, Pig, Bovine, Goat, Monkey, Chicken

Predicted Reactivity:

Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

GAPDH antibody detects endogenous levels of total GAPDH.

RRID:

AB_2839421
Please cite this product as: Affinity Biosciences Cat# AF7021, RRID:AB_2839421.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthesized peptide derived from human GAPDH.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

GAPDH

Molecular Weight:

Observed Mol.Wt.: 37kD.
Predicted Mol.Wt.: 36kDa(Calculated)..

Subcellular Location:

Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.

Description:

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) iswell known as one of the key enzymes involved in glycolysis.As well as functioning as a glycolytic enzyme in cytoplasm,recent evidence suggests that mammalian GAPDHis also involved in a great number of intracellular procesessuch as membrane fusion, microtubule bundling, phosphotransferaseactivity, nuclear RNA export, DNA replication,and DNA repair.

Sequence:
MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE

Research Background

Function:

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

Post-translational Modifications:

S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus (By similarity). S-nitrosylation of Cys-247 is induced by interferon-gamma and LDL(ox) implicating the iNOS-S100A8/9 transnitrosylase complex and seems to prevent interaction with phosphorylated RPL13A and to interfere with GAIT complex activity.

ISGylated.

Sulfhydration at Cys-152 increases catalytic activity.

Oxidative stress can promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process called nucleocytoplasmic coagulation. Such aggregates can be observed in vivo in the affected tissues of patients with Alzheimer disease or alcoholic liver cirrhosis, or in cell cultures during necrosis. Oxidation at Met-46 may play a pivotal role in the formation of these insoluble structures. This modification has been detected in vitro following treatment with free radical donor (+/-)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide. It has been proposed to destabilize nearby residues, increasing the likelihood of secondary oxidative damages, including oxidation of Tyr-45 and Met-105. This cascade of oxidations may augment GAPDH misfolding, leading to intermolecular disulfide cross-linking and aggregation.

Succination of Cys-152 and Cys-247 by the Krebs cycle intermediate fumarate, which leads to S-(2-succinyl)cysteine residues, inhibits glyceraldehyde-3-phosphate dehydrogenase activity. Fumarate concentration as well as succination of cysteine residues in GAPDH is significantly increased in muscle of diabetic mammals. It was proposed that the S-(2-succinyl)cysteine chemical modification may be a useful biomarker of mitochondrial and oxidative stress in diabetes and that succination of GAPDH and other thiol proteins by fumarate may contribute to the metabolic changes underlying the development of diabetes complications.

Subcellular Location:

Cytoplasm>Cytosol. Nucleus. Cytoplasm>Perinuclear region. Membrane. Cytoplasm>Cytoskeleton.
Note: Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with CHP1; the interaction increases the binding of CHP1 with microtubules. Associates with microtubules (By similarity). Interacts with EIF1AD, USP25, PRKCI and WARS1. Interacts with phosphorylated RPL13A; inhibited by oxidatively-modified low-densitity lipoprotein (LDL(ox)). Component of the GAIT complex. Interacts with FKBP6; leading to inhibit GAPDH catalytic activity.

Similarity:

The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Research Fields

Research Fields:

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.(View pathway)
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Metabolism > Global and overview maps > Biosynthesis of amino acids.

Protocols & Troubleshooting

Reference Citations:

1). Yang Z et al. Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation. Nat Biomed Eng 2019 Dec 16 (PubMed: 31844155) [IF=18.952]
2). Meng J et al. Twist1 Regulates Vimentin through Cul2 Circular RNA to Promote EMT in Hepatocellular Carcinoma. Cancer Res 2018 Aug 1;78(15):4150-4162 (PubMed: 29844124) [IF=9.727]
3). Han J et al. YY1 complex promotes Quaking expression via super-enhancer binding during EMT of hepatocellular carcinoma. Cancer Res 2019 Feb 13 (PubMed: 30760518) [IF=9.727]
4). Wei S;Qiu T;Yao X;Wang N;Jiang L;Jia X;Tao Y;Wang Z;Pei P;Zhang J;Zhu Y;Yang G;Liu X;Liu S;Sun X; et al. Arsenic induces pancreatic dysfunction and ferroptosis via mitochondrial ROS-autophagy-lysosomal pathway. J Hazard Mater 2020 Feb 15;384:121390. (PubMed: 31735470) [IF=9.038]
5). Jia X;Qiu T;Yao X;Jiang L;Wang N;Wei S;Tao Y;Pei P;Wang Z;Zhang J;Zhu Y;Yang G;Liu X;Liu S;Sun X; et al. Arsenic induces hepatic insulin resistance via mtROS-NLRP3 inflammasome pathway. J Hazard Mater 2020 Jun 4;399:123034. (PubMed: 32544768) [IF=9.038]
6). Xie-an Yu et al. A cancer-specific activatable theranostic nanodrug for enhanced therapeutic efficacy via amplification of oxidative stress. Theranostics 2020, Vol. 10, Issue 1 [IF=8.579]
7). Fan L;Lei H;Zhang S;Peng Y;Fu C;Shu G;Yin G; et al. Non-canonical signaling pathway of SNAI2 induces EMT in ovarian cancer cells by suppressing miR-222-3p transcription and upregulating PDCD10. Theranostics 2020 Apr 27;10(13):5895-5913. (PubMed: 32483426) [IF=8.579]
8). Wang JJ;Tian Y;Li MH;Feng YQ;Kong L;Zhang FL;Shen W; et al. Single-cell transcriptome dissection of the toxic impact of Di (2-ethylhexyl) phthalate on primordial follicle assembly. Theranostics 2021 Mar 5;11(10):4992-5009. (PubMed: 33754040) [IF=8.579]
9). Zhou Zhu et al. Micro or nano: Evaluation of biosafety and biopotency of magnesium metal organic framework-74 with different particle sizes. Nano Res 2020 Jan 16 [IF=8.183]
10). Xing Y et al. Dimerization of MICU Proteins Controls Ca2+ Influx through the Mitochondrial Ca2+ Uniporter. Cell Rep 2019 Jan 29;26(5):1203-1212 (PubMed: 30699349) [IF=8.109]
11). Meng J et al. Hsp90β promotes aggressive vasculogenic mimicry via epithelial-mesenchymal transition in hepatocellular carcinoma. Oncogene 2018 Aug 7 (PubMed: 30087438) [IF=7.971]
12). Liu L;Liang L;Yang C;Zhou Y;Chen Y; et al. Extracellular vesicles of Fusobacterium nucleatum compromise intestinal barrier through targeting RIPK1-mediated cell death pathway. Gut Microbes Jan-Dec 2021;13(1):1-20. (PubMed: 33769187) [IF=7.740]
13). Zhang W;Wang Q;Xing X;Yang L;Xu M;Cao C;Wang R;Li W;Niu X;Gao D; et al. The antagonistic effects and mechanisms of microRNA‐26a action in hypertensive vascular remodeling. Br J Pharmacol 2020 Dec 11. (PubMed: 33305374) [IF=7.730]
14). Hou H;Su K;Huang C;Yuan Q;Li S;Sun J;Lin Y;Du Z;Ke C;Yuan Z; et al. TRAIL‐Armed ER Nanosomes Induce Drastically Enhanced Apoptosis in Resistant Tumor in Combination with the Antagonist of IAPs (AZD5582). Adv Healthc Mater 2021 Jun;10(11):e2100030. (PubMed: 33963815) [IF=7.367]
15). Chen J et al. circPTN sponges miR-145-5p/miR-330-5p to promote proliferation and stemness in glioma. J Exp Clin Cancer Res 2019 Sep 11;38(1):398 (PubMed: 31511040) [IF=7.068]
16). Ye F et al. circFBXW7 Inhibits Malignant Progression by Sponging miR-197-3p and Encoding a 185-aa Protein in Triple-Negative Breast Cancer. Mol Ther Nucleic Acids 2019 Aug 14;18:88-98 (PubMed: 31536884) [IF=7.032]
17). Hu L;Liu J;Xue H;Panayi AC;Xie X;Lin Z;Wang T;Xiong Y;Hu Y;Yan C;Chen L;Abududilibaier A;Zhou W;Mi B;Liu G; et al. miRNA-92a-3p regulates osteoblast differentiation in patients with concomitant limb fractures and TBI via IBSP/PI3K-AKT inhibition. Mol Ther Nucleic Acids 2021 Feb 15;23:1345-1359. (PubMed: 33717654) [IF=7.032]
18). Li X et al. CircRNA104250 and lncRNAuc001.dgp.1 promote the PM2.5-induced inflammatory response by co-targeting miR-3607-5p in BEAS-2B cells. Environ Pollut 2019 Dec 7;258:113749 (PubMed: 31864925) [IF=6.792]
19). Liu S et al. CXCL13/CXCR5 Signaling Contributes to Diabetes-induced Tactile Allodynia via Activating pERK, pSTAT3, pAKT Pathways and Pro-inflammatory Cytokines Production in the Spinal Cord of male mice. Brain Behav Immun 2019 May 14 (PubMed: 31100371) [IF=6.633]
20). Zhao Z;Xue F;Gu Y;Han J;Jia Y;Ye K;Zhang Y; et al. Crosstalk between the muscular estrogen receptor α and BDNF/TrkB signaling alleviates metabolic syndrome via 7, 8-dihydroxyflavone in female mice. Mol Metab 2020 Dec 19;101149. (PubMed: 33352311) [IF=6.448]
21). Zhang Q et al. Thymidine phosphorylase promotes malignant progression in hepatocellular carcinoma through pentose Warburg effect. Cell Death Dis 2019 Jan 17;10(2):43 (PubMed: 30674871) [IF=6.304]
22). Fu R et al. Avenanthramide A triggers potent ROS-mediated anti-tumor effects in colorectal cancer by directly targeting DDX3. Cell Death Dis 2019 Aug 7;10(8):593 (PubMed: 31391454) [IF=6.304]
23). Yang L et al. Protopanaxadiol inhibits epithelial-mesenchymal transition of hepatocellular carcinoma by targeting STAT3 pathway. Cell Death Dis 2019 Aug 20;10(9):630 (PubMed: 31431619) [IF=6.304]
24). Duan X et al. Inhibition of keratinocyte necroptosis mediated by RIPK1/RIPK3/MLKL provides a protective effect against psoriatic inflammation. Cell Death Dis 2020 Feb 19;11(2):134 (PubMed: 32075957) [IF=6.304]
25). Zhu H et al. CircGCN1L1 promotes synoviocyte proliferation and chondrocyte apoptosis by targeting miR-330-3p and TNF-α in TMJ osteoarthritis. Cell Death Dis 2020 Apr 24;11(4):284 (PubMed: 32332704) [IF=6.304]
26). Chen Z;Wu C;Liu Y;Li H;Zhu Y;Huang C;Lin H;Qiao Q;Huang M;Zhu Q;Wang L; et al. ELABELA attenuates deoxycorticosterone acetate/salt-induced hypertension and renal injury by inhibition of NADPH oxidase/ROS/NLRP3 inflammasome pathway. Cell Death Dis 2020 Aug 22;11(8):698. (PubMed: 32829380) [IF=6.304]
27). He XF;Xu JH;Li G;Li MY;Li LL;Pei Z;Zhang LY;Hu XQ; et al. NLRP3-dependent microglial training impaired the clearance of amyloid-beta and aggravated the cognitive decline in Alzheimer’s disease. Cell Death Dis 2020 Oct 13;11(10):849. (PubMed: 33051464) [IF=6.304]
28). Cheng J;Li Y;Wang X;Dong G;Sheng C; et al. Discovery of Novel PDEδ Degraders for the Treatment of KRAS Mutant Colorectal Cancer. J Med Chem 2020 Jul 14. (PubMed: 32603594) [IF=6.205]
29). Lu C;Fan L;Zhang PF;Tao WW;Yang CB;Shang EX;Chen FY;Che CT;Cheng HB;Duan JA;Zhao M; et al. A novel P38α MAPK activator Bruceine A exhibits potent anti-pancreatic cancer activity. Comput Struct Biotechnol J 2021 Jun 6;19:3437-3450. (PubMed: 34194669) [IF=6.018]
30). Chen S;Lv K;Sharda A;Deng J;Zeng W;Zhang C;Hu Q;Jin P;Yao G;Xu X;Ming Z;Fang C; et al. Anti-Thrombotic Effects Mediated by Dihydromyricetin Involve Both Platelet Inhibition and Endothelial Protection. Pharmacol Res 2021 Mar 9;105540. (PubMed: 33711433) [IF=5.893]
31). Liu Y;Wang Z;Ju M;Zhao Y;Jing Y;Li J;Shao C;Fu T;Lv Z;Li G; et al. Modification of COL1A1 in autologous adipose tissue‐derived progenitor cells rescues the bone phenotype in a mouse model of osteogenesis imperfecta. J Bone Miner Res 2021 May 5. (PubMed: 33950576) [IF=5.854]
32). Tao Y et al. Autophagic-CTSB-inflammasome axis modulates hepatic stellate cells activation in arsenic-induced liver fibrosis. Chemosphere 2019 Sep 24;242:124959 (PubMed: 31669990) [IF=5.778]
33). Ke W;Wang B;Hua W;Song Y;Lu S;Luo R;Li G;Wang K;Liao Z;Xiang Q;Li S;Wu X;Zhang Y;Yang C; et al. The distinct roles of myosin IIA and IIB under compression stress in nucleus pulposus cells. Cell Prolif 2021 Jan 7;e12987. (PubMed: 33415745) [IF=5.753]
34). Li S;Luo L;He Y;Li R;Xiang Y;Xing Z;Li Y;Albashari AA;Liao X;Zhang K;Gao L;Ye Q; et al. Dental pulp stem cell‐derived exosomes alleviate cerebral ischaemia‐reperfusion injury through suppressing inflammatory response. Cell Prolif 2021 Aug;54(8):e13093. (PubMed: 34231932) [IF=5.753]
35). Wang H et al. Oleanolic acid inhibits epithelial-mesenchymal transition of hepatocellular carcinoma by promoting iNOS dimerization. Mol Cancer Ther 2018 Oct 8 (PubMed: 30297361) [IF=5.615]
36). Liu H et al. Antimalarial Drug Pyrimethamine Plays a Dual Role in Antitumor Proliferation and Metastasis through Targeting DHFR and TP. Mol Cancer Ther 2019 Mar;18(3):541-555 (PubMed: 30642883) [IF=5.615]
37). Wu L et al. Morin reduces inflammatory responses and alleviates lipid accumulation in hepatocytes. J Cell Physiol 2019 Apr 1 (PubMed: 30937936) [IF=5.546]
38). Tao Y;Qiu T;Yao X;Jiang L;Wang N;Jiang J;Jia X;Wei S;Zhang J;Zhu Y;Tian W;Yang G;Liu X;Liu S;Ding Y;Sun X; et al. IRE1α/NOX4 signaling pathway mediates ROS‐dependent activation of hepatic stellate cells in NaAsO2‐induced liver fibrosis. J Cell Physiol 2020 Aug 10. (PubMed: 32776539) [IF=5.546]
39). Duan Z;Yuan C;Han Y;Zhou L;Zhao J;Ruan Y;Chen J;Ni M;Ji X; et al. TMT-based quantitative proteomics analysis reveals the attenuated replication mechanism of Newcastle disease virus caused by nuclear localization signal mutation in viral matrix protein. Virulence 2020 May 18. (PubMed: 32420802) [IF=5.542]
40). Yu C et al. IL-17A promotes fatty acid uptake through the IL-17A/IL-17RA/p-STAT3/FABP4 axis to fuel ovarian cancer growth in an adipocyte-rich microenvironment. Cancer Immunol Immunother 2019 Dec 4 (PubMed: 31802182) [IF=5.442]
41). Ding Q et al. The role of the apoptosis-related protein BCL-B in the regulation of mitophagy in hepatic stellate cells during the regression of liver fibrosis. Exp Mol Med 2019 Jan 11;51(1):6 (PubMed: 30635551) [IF=5.418]
42). Adetula AA;Fan X;Zhang Y;Yao Y;Yan J;Chen M;Tang Y;Liu Y;Yi G;Li K;Tang Z; et al. Landscape of tissue-specific RNA Editome provides insight into co-regulated and altered gene expression in pigs (Sus-scrofa). RNA Biol 2021 Jul 27;1-12. (PubMed: 34314293) [IF=5.350]
43). Hu B;Zhang S;Liu W;Wang P;Chen S;Lv X;Shi D;Ma K;Wang B;Wu Y;Shao Z; et al. Inhibiting Heat Shock Protein 90 Protects Nucleus Pulposus-Derived Stem/Progenitor Cells From Compression-Induced Necroptosis and Apoptosis. Front Cell Dev Biol 2020 Aug 7;8:685. (PubMed: 32850811) [IF=5.201]
44). Chen S;Liang H;Ji Y;Kou H;Zhang C;Shang G;Shang C;Song Z;Yang L;Liu L;Wang Y;Liu H; et al. Curcumin Modulates the Crosstalk Between Macrophages and Bone Mesenchymal Stem Cells to Ameliorate Osteogenesis. Front Cell Dev Biol 2021 Feb 9;9:634650. (PubMed: 33634135) [IF=5.201]
45). Chen C et al. A multifunctional-targeted nanoagent for dual-mode image-guided therapeutic effects on ovarian cancer cells. Int J Nanomedicine 2019 Jan 21;14:753-769 (PubMed: 30718954) [IF=5.115]
46). Yuan K;Mei J;Shao D;Zhou F;Qiao H;Liang Y;Li K;Tang T; et al. Cerium Oxide Nanoparticles Regulate Osteoclast Differentiation Bidirectionally by Modulating the Cellular Production of Reactive Oxygen Species. Int J Nanomedicine 2020 Aug 25;15:6355-6372. (PubMed: 32922006) [IF=5.115]
47). Tian Y et al. Magnolol Alleviates Inflammatory Responses and Lipid Accumulation by AMP-Activated Protein Kinase-Dependent Peroxisome Proliferator-Activated Receptor α Activation. Front Immunol 2018 Feb 5;9:147 (PubMed: 29467759) [IF=5.085]
48). Zhou WJ;Wang DD;Tao J;Tai Y;Zhou ZW;Wang Z;Guo PP;Sun WY;Chen JY;Wu HX;Yan SX;Zhang LL;Wang QT;Wei W; et al. Deficiency of β-arrestin2 exacerbates inflammatory arthritis by facilitating plasma cell formation. Acta Pharmacol Sin 2020 Aug 27. (PubMed: 32855529) [IF=5.064]
49). He T et al. Casein kinase 1 epsilon facilitates cartilage destruction in osteoarthritis through JNK pathway. FASEB J 2020 Mar 16 (PubMed: 32175635) [IF=4.966]
50). Du P;Hu T;An Z;Li P;Liu L; et al. In vitro and in vivo synergistic efficacy of ceritinib combined with programmed cell death ligand‐1 inhibitor in anaplastic lymphoma kinase‐rearranged non‐small‐cell lung cancer. Cancer Sci 2020 Jun;111(6):1887-1898. (PubMed: 32227409) [IF=4.966]
51). Ke Q;Yang J;Liu H;Huang Z;Bu L;Jin D;Liu C; et al. Dose-and time-effects responses of Nonylphenol on oxidative stress in rat through the Keap1-Nrf2 signaling pathway. Ecotoxicol Environ Saf 2021 Apr 6;216:112185. (PubMed: 33836420) [IF=4.872]
52). Cai S et al. Glycyrrhizic Acid-Induced Differentiation Repressed Stemness in Hepatocellular Carcinoma by Targeting c-Jun N-Terminal Kinase 1. Front Oncol 2020 Jan 9;9:1431 (PubMed: 31998631) [IF=4.848]
53). Shi L et al. Cellular senescence induced by S100A9 in mesenchymal stromal cells through NLRP3 inflammasome activation. Aging (Albany NY) 2019 Nov 14;11(21):9626-9642 (PubMed: 31727865) [IF=4.831]
54). Jiang M;Liu J;Liu W;Zhu X;Bano Y;Liao H;Li H;Jiang HH; et al. Bone marrow stem cells secretome accelerates simulated birth trauma-induced stress urinary incontinence recovery in rats. Aging (Albany NY) 2021 Mar 31;13(7):10517-10534. (PubMed: 33793419) [IF=4.831]
55). Han B;Wang L;Yu S;Ge W;Li Y;Jiang H;Shen W;Sun Z; et al. One potential biomarker for teratozoospermia identified by in-depth integrative analysis of multiple microarray data. Aging (Albany NY) 2021 Mar 26;13(7):10208-10224. (PubMed: 33819193) [IF=4.831]
56). Luo DD et al. Tetrahydrocurcumin and octahydrocurcumin, the primary and final hydrogenated metabolites of curcumin, possess superior hepatic-protective effect against acetaminophen-induced liver injury: Role of CYP2E1 and Keap1-Nrf2 pathway. Food Chem Toxicol 2018 Nov 10 (PubMed: 30423402) [IF=4.679]
57). Li X;Shao S;Li H;Bi Z;Zhang S;Wei Y;Bai J;Zhang R;Ma X;Ma B;Zhang L;Xie C;Ning W;Zhou H;Yang C; et al. Byakangelicin protects against carbon tetrachloride–induced liver injury and fibrosis in mice. J Cell Mol Med 2020 Jul 9. (PubMed: 32643868) [IF=4.658]
58). Mei Y;Liu B;Su H;Zhang H;Liu F;Ke Q;Sun X;Tan W; et al. Isosteviol sodium protects the cardiomyocyte response associated with the SIRT1/PGC‐1α pathway. J Cell Mol Med 2020 Aug 5. (PubMed: 32757458) [IF=4.658]
59). Liu H;Zhao Z;Wu T;Zhang Q;Lu F;Gu J;Jiang T;Xue J; et al. Inhibition of autophagy‐dependent pyroptosis attenuates cerebral ischaemia/reperfusion injury. J Cell Mol Med 2021 May 2. (PubMed: 33938129) [IF=4.658]
60). Wang X et al. Autophagy enhanced antitumor effect in K562 and K562/ADM cells using realgar transforming solution. Biomed Pharmacother 2018 Feb;98:252-264 (PubMed: 29272786) [IF=4.545]
61). Lian FZ et al. Xin-Ji-Er-Kang ameliorates kidney injury following myocardial infarction by inhibiting oxidative stress via Nrf2/HO-1 pathway in rats. Biomed Pharmacother 2019 Jun 19;117:109124 (PubMed: 31228798) [IF=4.545]
62). Wu J et al. Patchouli alcohol attenuates 5-fluorouracil-induced intestinal mucositis via TLR2/MyD88/NF-kB pathway and regulation of microbiota. Biomed Pharmacother 2020 Jan 28;124:109883 (PubMed: 32004938) [IF=4.545]
63). Ding Y;Wang L;Ji W;Chen Z;Wang D;Chen C;Tong H;Han Z;Niu C;Chu M;Huang J;Guo X; et al. Generation of a human induced pluripotent stem cell line with Cas9 driven by Tet-on operator via AAVS1 safe harbor gene-editing. Stem Cell Res 2020 Oct 27;49:102064. (PubMed: 33207306) [IF=4.489]
64). Liu H et al. Long non-coding RNA MALAT1 mediates hypoxia-induced pro-survival autophagy of endometrial stromal cells in endometriosis. J Cell Mol Med 2019 Jan;23(1):439-452 (PubMed: 30324652) [IF=4.486]
65). Xie J et al. circEPSTI1 regulates ovarian cancer progression via decoying miR-942. J Cell Mol Med 2019 Mar 19 (PubMed: 30887698) [IF=4.486]
66). Shen B et al. Aucubin inhibited lipid accumulation and oxidative stress via Nrf2/HO-1 and AMPK signalling pathways. J Cell Mol Med 2019 Apr 4 (PubMed: 30950217) [IF=4.486]
67). Li X;Shao S;Li H;Bi Z;Zhang S;Wei Y;Bai J;Zhang R;Ma X;Ma B;Zhang L;Xie C;Ning W;Zhou H;Yang C; et al. Byakangelicin protects against carbon tetrachloride–induced liver injury and fibrosis in mice. J Cell Mol Med 2020 Jul 9. (PubMed: 32643868) [IF=4.486]
68). Shen B et al. Geniposide alleviates non-alcohol fatty liver disease via regulating Nrf2/AMPK/mTOR signalling pathways. J Cell Mol Med 2020 Apr 15 (PubMed: 32293113) [IF=4.486]
69). Mei Y;Liu B;Su H;Zhang H;Liu F;Ke Q;Sun X;Tan W; et al. Isosteviol sodium protects the cardiomyocyte response associated with the SIRT1/PGC‐1α pathway. J Cell Mol Med 2020 Aug 5. (PubMed: 32757458) [IF=4.486]
70). Liu H;Zhao Z;Wu T;Zhang Q;Lu F;Gu J;Jiang T;Xue J; et al. Inhibition of autophagy‐dependent pyroptosis attenuates cerebral ischaemia/reperfusion injury. J Cell Mol Med 2021 May 2. (PubMed: 33938129) [IF=4.486]
71). Liu K et al. Melatonin reduces intramuscular fat deposition by promoting lipolysis and increasing mitochondrial function. J Lipid Res 2018 Dec 14 (PubMed: 30552289) [IF=4.483]
72). Yueyang Zhao et al. Dihydroartemisinin Ameliorates Learning and Memory in Alzheimer’s Disease Through Promoting Autophagosome-Lysosome Fusion and Autolysosomal Degradation for Aβ Clearance. Front Aging Neurosci 2020 Mar 2; [IF=4.362]
73). Hu J et al. Effects of Xin-Ji-Er-Kang on heart failure induced by myocardial infarction: Role of inflammation, oxidative stress and endothelial dysfunction. Phytomedicine 2018 Mar 15;42:245-257 (PubMed: 29655692) [IF=4.268]
74). Xiong H et al. Tibetan medicine Kuan-Jin-Teng exerts anti-arthritic effects on collagen-induced arthritis rats via inhibition the production of pro-inflammatory cytokines and down-regulation of MAPK signaling pathway. Phytomedicine 2018 Dec 18;57:271-281 (PubMed: 30802713) [IF=4.268]
75). Zhang QQ;Li GQ;Zhong Y;Wang J;Wang AN;Zhou X;Mao XM; et al. Empagliflozin improves chronic hypercortisolism-induced abnormal myocardial structure and cardiac function in mice. Ther Adv Chronic Dis 2020 Nov 25;11:2040622320974833. (PubMed: 33294147) [IF=4.257]
76). Zhou J et al. Adipose derived mesenchymal stem cells alleviated osteoarthritis and chondrocyte apoptosis through autophagy inducing. J Cell Biochem 2018 Oct 13 (PubMed: 30315711) [IF=4.237]
77). Qu M;Wang W;Li W;Cao J;Zhang X;Wang C;Wu J;Yu B;Zhang H;Wu H;Kong W;Yu X; et al. Antiviral Activity of Feline BCA2 Is Mainly Dependent on Its Interference With Proviral Transcription Rather Than Degradation of FIV Gag. Front Microbiol 2020 Jun 11;11:1230. (PubMed: 32595622) [IF=4.235]
78). Li MY et al. Anti-Inflammatory Effects of Huangqin Decoction on Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice Through Regulation of the Gut Microbiota and Suppression of the Ras-PI3K-Akt-HIF-1α and NF-κB Pathways. Front Pharmacol 2020 Jan 20;10:1552 (PubMed: 32038240) [IF=4.225]
79). Cheng Y;Yang Y;Wu Y;Wang W;Xiao L;Zhang Y;Tang J;Huang YD;Zhang S;Xiang Q; et al. The Curcumin Derivative, H10, Suppresses Hormone-Dependent Prostate Cancer by Inhibiting 17β-Hydroxysteroid Dehydrogenase Type 3. Front Pharmacol 2020 May 8;11:637. (PubMed: 32457626) [IF=4.225]
80). Gu Y;Zhang Y;Li M;Huang Z;Jiang J;Chen Y;Chen J;Jia Y;Zhang L;Zhou F; et al. Ferulic acid ameliorates atherosclerotic injury by modulating gut microbiota and lipid metabolism. Front Pharmacol 2021 Mar 25;12:621339. (PubMed: 33841148) [IF=4.225]
81). Lim C et al. Ubiquitin-specific peptide 22 acts as an oncogene in gastric cancer in a son of sevenless 1-dependent manner. Cancer Cell Int 2020 Feb 10;20:45 (PubMed: 32063746) [IF=4.175]
82). Meng F;Zhang L;Zhang M;Ye K;Guo W;Liu Y;Yang W;Zhai Z;Wang H;Xiao J;Dai H; et al. Down-regulation of BCL2L13 renders poor prognosis in clear cell and papillary renal cell carcinoma. Cancer Cell Int 2021 Jun 30;21(1):332. (PubMed: 34193180) [IF=4.175]
83). Li X;Lu Z;Zhou F;Jin W;Yang Y;Chen S;Xie Z;Zhao Y; et al. Indoxyl sulfate promotes the atherosclerosis through up-regulating the miR-34a expression in endothelial cells and vascular smooth muscle cells in vitro. Vascul Pharmacol 2020 Jun 25;106763. (PubMed: 32593718) [IF=4.152]
84). Liu Y et al. A novel transgenic murine model with persistently brittle bones simulating osteogenesis imperfecta type I. Bone 2019 Jul 29 (PubMed: 31369917) [IF=4.147]
85). Seo E et al. Exendin-4 in combination with adipose-derived stem cells promotes angiogenesis and improves diabetic wound healing. J Transl Med 2017 Feb 15;15(1):35 (PubMed: 28202074) [IF=4.124]
86). Xu H et al. Involvement of insulin signalling pathway in methamphetamine-induced hyperphosphorylation of Tau. Toxicology 2018 Jul 4;408:88-94 (PubMed: 29981415) [IF=4.099]
87). Liu X et al. Lipoxin A4 and its analog suppress inflammation by modulating HMGB1 translocation and expression in psoriasis. Sci Rep 2017 Aug 2;7(1):7100 (PubMed: 28769106) [IF=3.998]

Application: WB    Species:human;    Sample:Not available

Figure 5. LXA4 pretreatment downregulates the LPS-induced secretion and expression of HMGB1 in keratinocytes. NHEKs were stimulated with LPS (10μg/ml) with and without preincubation with LXA4 (100nmol/l) for 30minutes. (A) Western blot analysis shows specifc bands for the expression of nuclear and cytoplasmic HMGB1 in NHEKs. Full-length blots are presented in Suppl.

88). Yuan Y et al. Regulation of Signaling Pathways Involved in the Anti-proliferative and Apoptosis-inducing Effects of M22 against Non-small Cell Lung Adenocarcinoma A549 Cells. Sci Rep 2018 Jan 17;8(1):992 (PubMed: 29343765) [IF=3.998]
89). Liang Y et al. A novel long non-coding RNA LINC00355 promotes proliferation of lung adenocarcinoma cells by down-regulating miR-195 and up-regulating the expression of CCNE1. Cell Signal 2019 Nov 2:109462 (PubMed: 31689506) [IF=3.968]
90). Wang J et al. PIM1 inhibitor SMI-4a attenuated lipopolysaccharide-induced acute lung injury through suppressing macrophage inflammatory responses via modulating p65 phosphorylation. Int Immunopharmacol 2019 Jun 13;73:568-574 (PubMed: 31203114) [IF=3.943]
91). Beng H et al. Differential effects of inhaled R- and S-terbutaline in ovalbumin-induced asthmatic mice. Int Immunopharmacol 2019 Aug;73:581-589 (PubMed: 31234092) [IF=3.943]
92). Liu S et al. Limonin ameliorates ulcerative colitis by regulating STAT3/miR-214 signaling pathway. Int Immunopharmacol 2019 Oct;75:105768 (PubMed: 31382166) [IF=3.943]
93). Liu S et al. Isosteroid alkaloids with different chemical structures from Fritillariae cirrhosae bulbus alleviate LPS-induced inflammatory response in RAW 264.7 cells by MAPK signaling pathway. Int Immunopharmacol 2019 Dec 6;78:106047 (PubMed: 31816576) [IF=3.943]
94). Jia H;Wu D;Zhang Z;Li S; et al. Regulatory effect of the MAFG‑AS1/miR‑150‑5p/MYB axis on the proliferation and migration of breast cancer cells. Int J Oncol 2020 Nov 20;58(1):33-44. (PubMed: 33367930) [IF=3.899]
95). Cheng RD;Ren W;Sun P;Tian L;Zhang L;Zhang J;Li JB;Ye XM; et al. Spinal cord injury causes insulin resistance associated with PI3K signaling pathway in hypothalamus. Neurochem Int 2020 Aug 24;140:104839. (PubMed: 32853751) [IF=3.881]
96). Ding CL et al. Identification of retinoid acid induced 16 as a novel androgen receptor target in prostate cancer cells. Mol Cell Endocrinol 2020 Jan 31:110745 (PubMed: 32014455) [IF=3.871]
97). Shi Y et al. Synaptic Plasticity of Human Umbilical Cord Mesenchymal Stem Cell Differentiating into Neuron-like Cells In Vitro Induced by Edaravone. Stem Cells Int 2018 Oct 28;2018:5304279 (PubMed: 30510585) [IF=3.869]
98). Zhang C et al. Transplantation of Human Urine-Derived Stem Cells Ameliorates Erectile Function and Cavernosal Endothelial Function by Promoting Autophagy of Corpus Cavernosal Endothelial Cells in Diabetic Erectile Dysfunction Rats. Stem Cells Int 2019 Sep 9;2019:2168709 (PubMed: 31582984) [IF=3.869]
99). Huang X et al. High glucose disrupts autophagy lysosomal pathway in gingival epithelial cells via ATP6V0C. J Periodontol 2019 Aug 30 (PubMed: 31471894) [IF=3.742]
100). Zhao C et al. β-Catenin Controls the Electrophysiologic Properties of Skeletal Muscle Cells by Regulating the α2 Isoform of Na+/K+-ATPase. Front Neurosci 2019 Aug 7;13:831 (PubMed: 31440132) [IF=3.707]
101). Fu WJ et al. Anti-atherosclerosis and cardio-protective effects of the Angong Niuhuang Pill on a high fat and vitamin D3 induced rodent model of atherosclerosis. J Ethnopharmacol 2017 Jan 4;195:118-126 (PubMed: 27880884) [IF=3.690]
102). Gan Y et al. Patchouli oil ameliorates 5-fluorouracil-induced intestinal mucositis in rats via protecting intestinal barrier and regulating water transport. J Ethnopharmacol 2019 Dec 25;250:112519 (PubMed: 31883475) [IF=3.690]
103). Liu T;Xie XL;Zhou X;Chen SX;Wang YJ;Shi LP;Chen SJ;Wang YJ;Wang SL;Zhang JN;Dou SY;Jiang XY;Cui RL;Jiang HQ; et al. Y-box binding protein 1 augments sorafenib resistance via the PI3K/Akt signaling pathway in hepatocellular carcinoma. World J Gastroenterol 2021 Jul 28;27(28):4667-4686. (PubMed: 34366628) [IF=3.665]
104). Zhou J et al. MicroRNA-145 overexpression attenuates apoptosis and increases matrix synthesis in nucleus pulposus cells. Life Sci 2019 Feb 20 (PubMed: 30797016) [IF=3.647]
105). Shen X;Zhao Y;Liu G;Zhou HL;Fan J;Zhang L;Li YL;Wang Y;Liang J;Xu ZX; et al. Upregulation of programmed death ligand 1 by liver kinase B1 and its implication in programmed death 1 blockade therapy in non-small cell lung cancer. Life Sci 2020 Jun 6;256:117923. (PubMed: 32522567) [IF=3.647]
106). Guo Y;Wang X;Jia P;You Y;Cheng Y;Deng H;Luo S;Huang B; et al. Ketogenic diet aggravates hypertension via NF-κB-mediated endothelial dysfunction in spontaneously hypertensive rats. Life Sci 2020 Jul 21;258:118124. (PubMed: 32702443) [IF=3.647]
107). Xun T et al. Advanced oxidation protein products upregulate efflux transporter expression and activity through activation of the Nrf-2-mediated signaling pathway in vitro and in vivo. Eur J Pharm Sci 2020 Apr 18:105342 (PubMed: 32315774) [IF=3.616]
108). Chen Z et al. Lower Expression of Gelsolin in Colon Cancer and Its Diagnostic Value in Colon Cancer Patients. J Cancer 2019 Jan 30;10(5):1288-1296 (PubMed: 30854138) [IF=3.565]
109). Zhong X;Huang S;Liu D;Jiang Z;Jin Q;Li C;Da L;Yao Q;Wang D; et al. Galangin promotes cell apoptosis through suppression of H19 expression in hepatocellular carcinoma cells. Cancer Med 2020 Jun 2. (PubMed: 32485786) [IF=3.491]
110). Wang DD;Li Y;Wu YJ;Wu YL;Han J;Olatunji OJ;Wang L;Zuo J; et al. Xanthones from securidaca inappendiculata antagonized the anti-rheumatic effects of methotrexate in vivo by promoting its secretion into urine. Expert Opin Drug Metab Toxicol 2020 Nov 12;1-10. (PubMed: 33107357) [IF=3.470]
111). Guo Q;Lin Y;Hu J; et al. Inhibition of miR-665-3p Enhances Autophagy and Alleviates Inflammation in Fusarium solani-Induced Keratitis. Invest Ophthalmol Vis Sci 2021 Jan 4;62(1):24. (PubMed: 33481985) [IF=3.470]
112). Cai W;Ni W;Jin Y;Li Y; et al. TRIP13 promotes lung cancer cell growth and metastasis through AKT/mTORC1/c-Myc signaling. Cancer Biomark 2020 Oct 23. (PubMed: 33136091) [IF=3.436]
113). Cong J;Cheng B;Liu J;He P; et al. RTEF-1 Inhibits Vascular Smooth Muscle Cell Calcification through Regulating Wnt/β-Catenin Signaling Pathway. Calcif Tissue Int 2021 Mar 13. (PubMed: 33713163) [IF=3.423]
114). Gao T et al. Aberrant hypomethylation and overexpression of the eyes absent homologue 2 suppresses tumor cell growth of human lung adenocarcinoma cells. Oncol Rep 2015 Nov;34(5):2333-42 (PubMed: 26329363) [IF=3.417]

Application: WB    Species:human;    Sample:NSCLC cells

Figure 1. Expression of EYA2 in NSCLC cells. (A) Western blot analysis. (B) EYA2 protein expression was significantly upregulated 3.2‑fold in the A549 cells compared to the BEAS-2B cells, but had no obvious change in the H661 and SKMES1 cells. (C) mRNA level of EYA2 exhibited a 2.1-fold upregulation in the A549 cells compared to the BEAS-2B cells. The groups were obtained from different parts of the same gel. * P

115). Cheng Z et al. Rab1A promotes proliferation and migration abilities via regulation of the HER2/AKT-independent mTOR/S6K1 pathway in colorectal cancer. Oncol Rep 2019 Mar 15 (PubMed: 30896866) [IF=3.417]
116). An Q;Liu T;Wang MY;Yang YJ;Zhang ZD;Liu ZJ;Yang B; et al. KRT7 promotes epithelial‑mesenchymal transition in ovarian cancer via the TGF‑β/Smad2/3 signaling pathway. Oncol Rep 2020 Dec 8;45(2):481-492. (PubMed: 33416175) [IF=3.417]
117). Ji S et al. Resveratrol promotes oxidative stress to drive DLC1 mediated cellular senescence in cancer cells. Exp Cell Res 2018 Sep 15;370(2):292-302 (PubMed: 29964052) [IF=3.383]
118). Lin FH;Wang A;Dai W;Chen S;Ding Y;Sun LV; et al. Lmod3 promotes myoblast differentiation and proliferation via the AKT and ERK pathways. Exp Cell Res 2020 Sep 25;396(2):112297. (PubMed: 32980291) [IF=3.383]
119). Bai XD et al. Constructing a better binding peptide for drug delivery targeting the interleukin-4 receptor. J Drug Target 2020 May 4:1-26 (PubMed: 32363946) [IF=3.380]
120). Liang R et al. Plasma amyloid beta level changes in aged mice with cognitive dysfunction following sevoflurane exposure. Exp Gerontol 2020 Jan;129:110737 (PubMed: 31521721) [IF=3.376]
121). Zhang QB;Ye RF;Ye LY;Zhang QY;Dai NG; et al. Isocorydine decrease gemcitabine-resistance by inhibiting epithelial-mesenchymal transition via STAT3 in pancreatic cancer cells. Am J Transl Res 2020 Jul 15;12(7):3702-3714. (PubMed: 32774728) [IF=3.375]
122). Chen WB;Zhang LX;Zhao YK;Li J;Jiao Y; et al. C/EBPα-mediated transcriptional activation of miR-134-5p entails KPNA3 inhibition and modulates focal hypoxic-ischemic brain damage in neonatal rats. Brain Res Bull 2020 Nov;164:350-360. (PubMed: 32814091) [IF=3.370]
123). Fang Q;Zheng B;Liu N;Liu J;Liu W;Huang X;Zeng X;Chen L;Li Z;Ouyang D; et al. Trimethylamine N-Oxide Exacerbates Renal Inflammation and Fibrosis in Rats With Diabetic Kidney Disease. Front Physiol 2021 Jun 16;12:682482. (PubMed: 34220546) [IF=3.367]
124). Zhai Z et al. Emerging Roles Of hsa-circ-0046600 Targeting The miR-640/HIF-1α Signalling Pathway In The Progression Of HCC. Onco Targets Ther 2019 Nov 6;12:9291-9302 (PubMed: 31807009) [IF=3.337]
125). Lei H;Ma Y;Tan J;Liu Q; et al. Helicobacter pylori Regulates the Apoptosis of Human Megakaryocyte Cells via NF-κB/IL-17 Signaling. Onco Targets Ther 2021 Mar 19;14:2065-2074. (PubMed: 33776453) [IF=3.337]
126). Liu H et al. Autophagy contributes to hypoxia-induced epithelial to mesenchymal transition of endometrial epithelial cells in endometriosis. Biol Reprod 2018 May 31 (PubMed: 29860279) [IF=3.322]
127). Wang KZ et al. Dietary gossypol reduced intestinal immunity and aggravated inflammation in on-growing grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol 2019 Mar;86:814-831 (PubMed: 30543935) [IF=3.298]
128). Li M;Hu FC;Qiao F;Du ZY;Zhang ML; et al. Sodium acetate alleviated high-carbohydrate induced intestinal inflammation by suppressing MAPK and NF-κB signaling pathways in Nile tilapia (Oreochromis niloticus). Fish Shellfish Immunol 2020 Mar;98:758-765. (PubMed: 31730927) [IF=3.298]
129). Ma W et al. MARVELD1 attenuates arsenic trioxide-induced apoptosis in liver cancer cells by inhibiting reactive oxygen species production. Ann Transl Med 2019 May;7(9):200 (PubMed: 31205918) [IF=3.297]
130). Lu K;Zhou J;Deng J;Li Y;Wu C;Bao J; et al. Periplaneta americana Oligosaccharides Exert Anti-Inflammatory Activity through Immunoregulation and Modulation of Gut Microbiota in Acute Colitis Mice Model. Molecules 2021 Mar 19;26(6):1718. (PubMed: 33808686) [IF=3.267]
131). Zhang J et al. Electrophysiological and trafficking defects of the SCN5A T353I mutation in Brugada syndrome are rescued by alpha-allocryptopine. Eur J Pharmacol 2015 Jan 5;746:333-43 (PubMed: 25261036) [IF=3.263]

Application: WB    Species:Not available;    Sample:Not available

132). Ren H et al. Endostatin attenuates PDGF-BB- or TGF-β1-induced HSCs activation via suppressing RhoA/ROCK1 signal pathways. Drug Des Devel Ther 2019 Jan 11;13:285-290 (PubMed: 30666090) [IF=3.216]
133). [No authors listed] et al. Erratum: Endostatin attenuates PDGF-BB- or TGF-β1-induced HSCs activation via suppressing RhoA/ROCK1 signal pathways [Erratum]. Drug Des Devel Ther 2019 Jan 31;13:499-500 (PubMed: 30787592) [IF=3.216]
134). Yang K et al. Metabolomics Analysis Reveals Therapeutic Effects of α-Mangostin on Collagen-Induced Arthritis in Rats by Down-regulating Nicotinamide Phosphoribosyltransferase. Inflammation 2019 Apr;42(2):741-753 (PubMed: 30484004) [IF=3.212]
135). Liu H et al. Hypoxia-inducible factor-1α promotes endometrial stromal cells migration and invasion by upregulating autophagy in endometriosis. Reproduction 2017 Jun;153(6):809-820 (PubMed: 28348069) [IF=3.206]

Application: WB    Species:human;    Sample:Not available

FIGURE 4. Knockdown of HIF-1α interferes with hypoxia-induced autophagy in HESCs. (A) Representative western blots of HIF-1α, Beclin1 and LC3 protein in HESCs transfected with scrambled control siRNA or HIF- 1α specific siRNA in the presence or absence of hypoxia. (B) The protein expression levels were quantified by Image J software and normalized to GAPDH protein levels. The data are presented as the means ± SD from at least three independent experiments (*p<0.05;**p<0.01; ***p

136). Li N et al. Notch activity mediates oestrogen-induced stromal cell invasion in endometriosis. Reproduction 2019 Feb 1 (PubMed: 30753135) [IF=3.206]
137). Li A et al. NUPR1 Silencing Induces Autophagy-Mediated Apoptosis in Multiple Myeloma Cells Through the PI3K/AKT/mTOR Pathway. DNA Cell Biol 2020 Jan 22 (PubMed: 31971825) [IF=3.191]
138). Liu X et al. The role of Delta-like 4 ligand/Notch-ephrin-B2 cascade in the pathogenesis of preeclampsia by regulating functions of endothelial progenitor cell. Placenta 2015 Sep;36(9):1002-10 (PubMed: 26212082) [IF=3.177]

Application: WB    Species:human;    Sample:human

Fig. 2. Transfection and reagents influenced the expression of ephrin-B2 in EPCs.

139). Liu W et al. dNK derived IFN-γ mediates VSMC migration and apoptosis via the induction of LncRNA MEG3: A role in uterovascular transformation. Placenta 2017 Feb;50:32-39 (PubMed: 28161059) [IF=3.177]
140). Sun L et al. Effects of high-intensity interval training on adipose tissue lipolysis, inflammation, and metabolomics in aged rats. Pflugers Arch 2020 Feb;472(2):245-258 (PubMed: 32006095) [IF=3.158]
141). Zhan G et al. PGC-1α-FNDC5-BDNF signaling pathway in skeletal muscle confers resilience to stress in mice subjected to chronic social defeat. Psychopharmacology (Berl) 2018 Sep 26 (PubMed: 30259075) [IF=3.130]
142). Gao T et al. Interleukin-4 signalling pathway underlies the anxiolytic effect induced by 3-deoxyadenosine. Psychopharmacology (Berl) 2019 Apr 8 (PubMed: 30963194) [IF=3.130]
143). Huang N et al. Contribution of skeletal muscular glycine to rapid antidepressant effects of ketamine in an inflammation-induced mouse model of depression. Psychopharmacology (Berl) 2019 Jul 18 (PubMed: 31321459) [IF=3.130]
144). Liu SY et al. Fibroblast growth factor-23 may serve as a novel biomarker for renal osteodystrophy progression. Int J Mol Med 2018 Oct 15 (PubMed: 30365152) [IF=3.098]
145). Liao ZL et al. Salidroside protects PC-12 cells against amyloid β-induced apoptosis by activation of the ERK1/2 and AKT signaling pathways. Int J Mol Med 2019 Feb 1 (PubMed: 30720058) [IF=3.098]
146). Wang X;Liu X;Liu N;Chen H; et al. Prediction of crucial epigenetically‑associated, differentially expressed genes by integrated bioinformatics analysis and the identification of S100A9 as a novel biomarker in psoriasis. Int J Mol Med 2020 Jan;45(1):93-102. (PubMed: 31746348) [IF=3.098]
147). Luo Z;Lin J;Sun Y;Wang C;Chen J; et al. Bone Marrow Stromal Cell-Derived Exosomes promote muscle healing following contusion through macrophage polarization. Stem Cells Dev 2020 Dec 15. (PubMed: 33323007) [IF=3.082]
148). Huang N et al. Alterations in the BDNF-mTOR Signaling Pathway in the Spinal Cord Contribute to Hyperalgesia in a Rodent Model of Chronic Restraint Stress. Neuroscience 2019 Mar 30 (PubMed: 30940563) [IF=3.056]
149). Su R et al. The potential immunotoxicity of fine particulate matter based on SD rat spleen. Environ Sci Pollut Res Int 2019 Aug;26(23):23958-23966 (PubMed: 31218585) [IF=3.056]
150). Cai Y et al. Intermittent Theta Burst Stimulation Improves the Spatial Cognitive Function of Rats with Chronic Hypertension-induced Cerebral Small Vessel Disease. Neuroscience 2020 Apr 27 (PubMed: 32353458) [IF=3.056]
151). Liu DH et al. Anticonvulsant and Neuroprotective Effects of Paeonol in Epileptic Rats. Neurochem Res 2019 Sep 13 (PubMed: 31520267) [IF=3.038]
152). Chen M et al. Long Noncoding RNA SNHG5 Knockdown Alleviates Neuropathic Pain by Targeting the miR-154-5p/CXCL13 Axis. Neurochem Res 2020 Apr 4 (PubMed: 32248399) [IF=3.038]
153). Luo Q;Zhang W;Liu X;Zheng Y;Gao H;Zhao Y;Zou L; et al. Delta-Like 4-Notch signaling regulates trophoblast migration and invasion by targeting EphrinB2. Biochem Biophys Res Commun 2020 Jul 5;527(4):915-921. (PubMed: 32430173)
154). Li R;Hou S;Zou M;Ye K;Xiang L; et al. miR-543 impairs cell proliferation, migration, and invasion in breast cancer by suppressing VCAN. Biochem Biophys Res Commun 2021 Jul 19;570:191-198. (PubMed: 34293593)
155). Wei SJ;Zhang Q;Xiang YJ;Peng LY;Peng W;Ren Q;Gao YX; et al. Guizhi-Shaoyao-Zhimu decoction attenuates bone erosion in rats that have collagen-induced arthritis via modulating NF‐κB signalling to suppress osteoclastogenesis. Pharm Biol 2021 Dec;59(1):262-274. (PubMed: 33626293)
156). Luo J;Mu J;Zhang M;Zhao B;Liu L; et al. SPAG6-silencing enhances decitabine-induced apoptosis and demethylation of PTEN in SKM-1 cells and in a xenograft mouse model. Leuk Lymphoma 2021 Apr 10;1-12. (PubMed: 33843428)
157). Pan L et al. The cardiac glycoside oleandrin induces apoptosis in human colon cancer cells via the mitochondrial pathway. Cancer Chemother Pharmacol 2017 Jul;80(1):91-100 (PubMed: 28597038)

Application: WB    Species:human;    Sample:SW480

158). Chen L et al. Methamphetamine exposure induces neuropathic protein β-Amyloid expression. Toxicol In Vitro 2019 Feb;54:304-309 (PubMed: 30367931)
159). Hu T;Luo Z;Li K;Wang S;Wu D; et al. Zanthoxylum nitidum extract attenuates BMP-2 induced inflammation and hyperpermeability. Biosci Rep 2020 Oct 8;BSR20201098. (PubMed: 33030503)
160). Jiang Y;Chen D;Gong Q;Xu Q;Pan D;Lu F;Tang Q; et al. Elucidation of SIRT-1/PGC-1α-associated mitochondrial dysfunction and autophagy in nonalcoholic fatty liver disease. Lipids Health Dis 2021 Apr 26;20(1):40. (PubMed: 33902605)
161). Zhao H et al. KIF15 promotes bladder cancer proliferation via the MEK-ERK signaling pathway. Cancer Manag Res 2019 Feb 26;11:1857-1868 (PubMed: 30881113)
162). Ding L et al. The protective effects of polysaccharide extract from Xin-Ji-Er-Kang formula on Ang II-induced HUVECs injury, L-NAME-induced hypertension and cardiovascular remodeling in mice. BMC Complement Altern Med 2019 Jun 13;19(1):127 (PubMed: 31196042)
163). Zhou Y et al. Magnesium ion leachables induce a conversion of contractile vascular smooth muscle cells to an inflammatory phenotype. J Biomed Mater Res B Appl Biomater 2018 Oct 1 (PubMed: 30270501)
164). Tao H et al. LncRNA MEG3 inhibits trophoblast invasion and trophoblast-mediated VSMC loss in uterine spiral artery remodeling. Mol Reprod Dev 2019 May 8 (PubMed: 31066488)
165). Zhang S;Liu W;Wang P;Hu B;Lv X;Chen S;Wang B;Shao Z; et al. Activation of HSP70 impedes tert-butyl hydroperoxide (t-BHP)-induced apoptosis and senescence of human nucleus pulposus stem cells via inhibiting the JNK/c-Jun pathway. Mol Cell Biochem 2021 Jan 28. (PubMed: 33511552)
166). Ma J et al. Locostatin Alleviates Liver Fibrosis Induced by Carbon Tetrachloride in Mice. Dig Dis Sci 2019 Sep;64(9):2570-2580 (PubMed: 30874989)
167). Su C et al. [EXPRESS] X-ray induces mechanical and heat allodynia in mouse via TRPA1 and TRPV1 activation. Mol Pain 2019 Apr 23:1744806919849201 (PubMed: 31012378)
168). Zhao X et al. Up-regulation of miR-21 and miR-23a Contributes to As2 O3 -induced hERG Channel Deficiency. Basic Clin Pharmacol Toxicol 2015 Jun;116(6):516-23 (PubMed: 25395240)

Application: WB    Species:Not available;    Sample:Not available

169). Xiao J;Chen X;Lu X;Xie M;He B;He S;You S;Chen Q; et al. Progesterone/Org inhibits lung adenocarcinoma cell growth via membrane progesterone receptor alpha. Thorac Cancer 2020 Jun 11. (PubMed: 32529777)
170). Ruan H et al. Anlotinib attenuated bleomycin-induced pulmonary fibrosis via the TGF-β1 signalling pathway. J Pharm Pharmacol 2019 Oct 28 (PubMed: 31659758)
171). Liu S et al. Isosteroid alkaloids from Fritillaria cirrhosa bulbus as inhibitors of cigarette smoke-induced oxidative stress. Fitoterapia 2020 Jan;140:104434 (PubMed: 31760067)
172). Chen D et al. Quxie Capsule Inhibits Colon Tumor Growth Partially Through Foxo1-Mediated Apoptosis and Immune Modulation. Integr Cancer Ther 2019 Jan-Dec;18:1534735419846377 (PubMed: 31030593)
173). Wu Q;Chen J;Hu X;Zhu Y;Xie S;Wu C;Pei Z;Xiong S;Peng Y; et al. Amphiregulin Alleviated Concanavalin A-induced Acute Liver Injury via IL-22. Immunopharmacol Immunotoxicol 2020 Aug 17;1-28. (PubMed: 32806961)
174). Zhang J et al. Long non-coding RNA NEAT1 regulates E2F3 expression by competitively binding to miR-377 in non-small cell lung cancer. Oncol Lett 2017 Oct;14(4):4983-4988 (PubMed: 29085511)
175). Liu Y et al. Endovascular hypothermia improves post-resuscitation myocardial dysfunction by increasing mitochondrial biogenesis in a pig model of cardiac arrest. Cryobiology 2019 Jul 6 (PubMed: 31283936)
176). Wang L et al. CDK3 is a major target of miR-150 in cell proliferation and anti-cancer effect. Exp Mol Pathol 2017 Apr;102(2):181-190 (PubMed: 28108217)

Application: WB    Species:human;    Sample:H1299 cells

Figure 6. miR-150 suppresses CDK3 expression in lung cancer compared cells. SPC1and H1299 cells were transfected with miR-150 mimics and mimics NC for forty-eight hours, respectively. A. Western blot of CDK3 in SPC1and H1299 cells. B. qRT-PCR analysis of miR-150 in SPC1and H1299 cells. C. qRT-PCR analysis of CDK3 mRNA in SPC1and H1299 cells. Data are the means ± SEM of three independent experiments. *p<0.05, **p

177). Liu T;Liang X;Yang S;Sun Y; et al. Long noncoding RNA PTCSC1 drives esophageal squamous cell carcinoma progression through activating Akt signaling. Exp Mol Pathol 2020 Dec;117:104543. (PubMed: 32971114)
178). Zhang YH;Chen AL;Yu RQ;Jia BB;Ye DN;Wang M;Mei YZ;Fang GD;Jiang SY;Zhou Q;Zhang B; et al. miR-296-5p Inhibits the Secretion of Pulmonary Surfactants in Pulmonary Epithelial Cells via the Downregulation of Wnt7b/β-Catenin Signaling. Biomed Res Int 2021 Jan 5;2021:4051504. (PubMed: 33490270)
179). Li A;Chen X;Jing Z;Chen J; et al. Trifluoperazine induces cellular apoptosis by inhibiting autophagy and targeting NUPR1 in multiple myeloma. FEBS Open Bio 2020 Aug 18. (PubMed: 32810364)
180). Mao X et al. Inhibitors of PARP-1 exert inhibitory effects on the biological characteristics of hepatocellular carcinoma cells in vitro. Mol Med Rep 2017 Jul;16(1):208-214 (PubMed: 28498459)

Application: WB    Species:human;    Sample:HepG2

Figure 4. Effects of different concentrations of AG014699 and BSI‑201 on protein levels of Caspase 3, Caspase 8, Bax and Bcl‑2 in HepG2 cells.(A) Blots showing proteins in cells treated with AGO14699 and (B) quantification. (C) Blots showing proteins in cells treated with (C) BSI‑201 and (D) quantification. * P<0.05, compared with the control group; ∆P<0.05, compared with the low dose group; ∆∆P<0.05, compared with the middle dose group. CTRL, control; Bcl‑2, B‑cell lymphoma 2; BAX, Bcl‑2‑associated X protein.

181). Wu Y et al. Study on the mechanism of JAK2/STAT3 signaling pathway-mediated inflammatory reaction after cerebral ischemia. Mol Med Rep 2018 Apr;17(4):5007-5012 (PubMed: 29393445)
182). Zhao WX et al. Notch2 and Notch3 suppress the proliferation and mediate invasion of trophoblast cell lines. Biol Open 2017 Aug 15;6(8):1123-1129 (PubMed: 28606936)
183). Yang J;Ding X;Meng S;Cai J;Zhou W; et al. The c.863A>G (p.Glu288Gly) variant of the CTSD gene is not associated with CLN10 disease. Mol Genet Genomic Med 2021 Jul 31;e1777. (PubMed: 34331747)
184). Zhao J;Han Y;Wang Z;Zhang R;Wang G;Mao Y; et al. Alginate oligosaccharide protects endothelial cells against oxidative stress injury via integrin-α/FAK/PI3K signaling. Biotechnol Lett 2020 Sep 28. (PubMed: 32986180)
185). Li Y;Wang C;Liu Y;You J;Su G; et al. Autophagy, lysosome dysfunction and mTOR inhibition in MNU-induced photoreceptor cell damage. Tissue Cell 2019 Dec;61:98-108. (PubMed: 31759414)
186). Jiang W et al. Identification of Key Candidate Genes and Pathways of Candida albicans-Infected Human Umbilical Vein Endothelial Cells and Drug Screening. Indian J Microbiol 2020 Mar;60(1):62-69 (PubMed: 32089575)
187). Kan J;Huang H;Jiang Z;Zhou R;Bai S;Liao C;Chen J;Dong J;Zhang Y;Zhang J;Zhang R;Zhou D;Zhang E; et al. Arenobufagin Promoted Oxidative Stress-Associated Mitochondrial Pathway Apoptosis in A549 Non-Small-Cell Lung Cancer Cell Line. Evid Based Complement Alternat Med 2020 Apr 22;2020:8909171. (PubMed: 32382311)
188). Li Y;Lu X;Li X;Guo X;Sheng Y;Li Y;Xu G;Han X;An L;Du P; et al. Effects of Agaricus blazei Murrill polysaccharides on hyperlipidemic rats by regulation of intestinal microflora. Food Sci Nutr 2020 Apr 19;8(6):2758-2772. (PubMed: 32566193)
189). Huo C;Wang L;Wang Q;Yang Y;Chen B; et al. Hydroxysafflor Yellow A inhibits the viability and migration of vascular smooth muscle cells induced by serum from rats with chronic renal failure via inactivation of the PI3K/Akt signaling pathway. Exp Ther Med 2021 Aug;22(2):850. (PubMed: 34149896)
190). Ali I;Li C;Li L;Kuang M;Shafiq M;Wang Y;Yang M;Wang G; et al. Effect of acetate, β-hydroxybutyrate and their interaction on lipogenic gene expression, triglyceride contents and lipid droplet formation in dairy cow mammary epithelial cells. In Vitro Cell Dev Biol Anim 2021 Jan 5. (PubMed: 33403623)
191). Xu Y et al. Novel conjugates of endoperoxide and 4-anilinoquinazoline induce myeloma cell apoptosis by inhibiting the IGF1-R/AKT/mTOR signaling pathway. Biosci Trends 2020 Mar 13 (PubMed: 32173687)
192). Luo ML et al. Conditioned Medium from Human Umbilical Vein Endothelial Cells Promotes Proliferation, Migration, Invasion and Angiogenesis of Adipose Derived Stem Cells. Curr Med Sci 2018 Feb;38(1):124-130 (PubMed: 30074161)
193). Ye R et al. Stress causes cognitive impairment by affecting cholesterol efflux and reuptake leading to abnormalities in lipid metabolism of rats. J Integr Neurosci 2020 Mar 30;19(1):39-49 (PubMed: 32259885)
194). Yuan H;Liu F;Ma T;Zeng Z;Zhang N; et al. miR-338-3p inhibits cell growth, invasion, and EMT process in neuroblastoma through targeting MMP-2. Open Life Sci 2021 Mar 5;16(1):198-209. (PubMed: 33817311)
195). Xiang XJ et al. MiR-1271 Inhibits Cell Proliferation, Invasion and EMT in Gastric Cancer by Targeting FOXQ1. Cell Physiol Biochem 2015;36(4):1382-94 (PubMed: 26159618)

Application: WB    Species:human;    Sample:human gastric cancer

Fig. 2. FOXQ1 is a direct target of miR-1271. (A) TargetScan (www.targetscan. org) and miRanda (www. microRNA.org) showed that FOXQ1 was a direct target of miR-1271. (B) The luciferase reporter assay showed that FOXQ1 could be targeted by miR-1271 in MGC- 803 and SGC-7901 cells. (C) The qPCR and western blot analyses showed that miR-1271 mimics could inhibit the mRNA and protein expression of FOXQ1 in MGC-803 cells, whereas the miR-1271 inhibitor could increase the mRNA and protein expression of FOXQ1 in SGC-7901 cells. Three independent experiments were conducted. *P < 0.05, **P < 0.01.

196). Liu YR et al. Twist1 confers multidrug resistance in colon cancer through upregulation of ATP-binding cassette transporters. Oncotarget 2017 May 2;8(32):52901-52912 (PubMed: 28881781)
197). Li XH et al. Parthenolide attenuated bleomycin-induced pulmonary fibrosis via the NF-κB/Snail signaling pathway. Respir Res 2018 Jun 5;19(1):111 (PubMed: 29871641)
198). Sun C et al. Hsa-miR-326 targets CCND1 and inhibits non-small cell lung cancer development. Oncotarget 2016 Feb 16;7(7):8341-59 (PubMed: 26840018)

Application: WB    Species:Not available;    Sample:Not available

Figure 8: Ectopic expression of miR-326 promotes apoptosis in A549 and SPC-A-1 cells. (C) Western-blot of Bcl2 protein in A549 and SPC-A-1 cells after transfection.

199). Xin XL et al. Mechanisms of IFNalpha-1a-Induced Apoptosis in a Laryngeal Cancer Cell Line. Med Sci Monit 2019 Sep 22;25:7100-7114 (PubMed: 31542790)
200). Li S;Li Z;Guo T;Xing XF;Cheng X;Du H;Wen XZ;Ji JF; et al. Maternal embryonic leucine zipper kinase serves as a poor prognosis marker and therapeutic target in osteosarcoma. Oncotarget 2016 Feb 2;7(5):6266-80. (PubMed: 26701722)
201). Du P;Hu T;An Z;Li P;Liu L; et al. In vitro and in vivo synergistic efficacy of ceritinib combined with programmed cell death ligand‐1 inhibitor in anaplastic lymphoma kinase‐rearranged non‐small‐cell lung cancer. Cancer Sci 2020 Jun;111(6):1887-1898. (PubMed: 32227409)
202). Sun H et al. Role of 5-HT1A receptor in insular cortex mediating stress - induced visceral sensory dysfunction. Neurogastroenterol Motil 2016 Jul;28(7):1104-13 (PubMed: 26969829)

Application: WB    Species:Not available;    Sample:Not available

Figure 3 Comparison of the expression levels of HTR1As and NR2B in bilateral insular cortexes among three groups. The mRNA and protein expression levels of HTR1As (A and C) and NR2B (B and D) in bilateral insular cortexes were compared among WAS, sham WAS and normal control groups. (E) and (F): Representative immunoblots from the bilateral insular cortexes show the expression of NR2B, 5HTR1A and GAPDH, with an indication of size (kilodaltons, kDa). *represented that the difference was significant, p < 0.05. WAS, water avoidance stress.

203). Xiulong Niu et al. IL-17A exacerbates cisplatin-based resistance of OVCA via upregulating the expression of ABCG2 and MDR1 through Gli1-mediated Hh signaling. Oncotarget 2016
204). et al. Cdk5-Mediated Drp1 Phosphorylation Drives Mitochondrial Defects and Neuronal Apoptosis in Radiation-Induced Optic Neuropathy.
205). et al. Evaluation of curcumin-mediated photodynamic therapy on the reverse of multidrug resistance in tumor cells.
206). Wang H;Zhang S;Yang F;Xin R;Wang S;Cui D;Sun Y; et al. The gut microbiota confers protection in the CNS against neurodegeneration induced by manganism. Biomed Pharmacother 2020 Apr 21;127:110150. (PubMed: 32330797)
207). Zhao L;Bi M;Zhang H;Shi M; et al. Downregulation of NEAT1 Suppresses Cell Proliferation, Migration, and Invasion in NSCLC Via Sponging miR-153-3p. Cancer Biother Radiopharm 2020 May 7. (PubMed: 32380843)
208). Lu Z;Lu F;Wu L;He B;Chen Z;Yan M; et al. Berberine attenuates non-alcoholic steatohepatitis by regulating chemerin/CMKLR1 signalling pathway and Treg/Th17 ratio. Naunyn Schmiedebergs Arch Pharmacol 2020 Jun 10. (PubMed: 32524150)
209). et al. Regulation of RUNX3 expression by DNA methylation in prostate cancer.
210). et al. NUSAP1 Promotes Gastric Cancer Progression Through Regulation of Yap Stability.
211). Liu Y;Ju M;Wang Z;Li J;Shao C;Fu T;Jing Y;Zhao Y;Lv Z;Li G; et al. The synergistic effect of NELL1 and adipose-derived stem cells on promoting bone formation in osteogenesis imperfecta treatment. Biomed Pharmacother 2020 May 23;128:110235. (PubMed: 32454289)
212). et al. Ellagic Acid Attenuates BLM-Induced Pulmonary Fibrosis via Inhibiting Wnt Signaling Pathway.
213). Zhou W;Hu M;Zang X;Liu Q;Du J;Hu J;Zhang L;Du Z;Xiang Z; et al. Luteolin attenuates imiquimod–induced psoriasis-like skin lesions in BALB/c mice via suppression of inflammation response. Biomed Pharmacother 2020 Sep 10;131:110696. (PubMed: 32920513)
214). Wang H;Yang F;Xin R;Cui D;He J;Zhang S;Sun Y; et al. The gut microbiota attenuate neuroinflammation in manganese exposure by inhibiting cerebral NLRP3 inflammasome. Biomed Pharmacother 2020 Sep;129:110449. (PubMed: 32768944)
215). Guo Y;Ran Z;Zhang Y;Song Z;Wang L;Yao L;Zhang M;Xin J;Mao X; et al. Marein ameliorates diabetic nephropathy by inhibiting renal sodium glucose transporter 2 and activating the AMPK signaling pathway in db/db mice and high glucose–treated HK-2 cells. Biomed Pharmacother 2020 Nov;131:110684. (PubMed: 33152903)
216). et al. The MYC Paralog-PARP1 Axis as a Potential Therapeutic Target in MYC Paralog-Activated Small Cell Lung Cancer.
217). et al. AKAP95 transports Cx43 into nucleus, regulating G1/S transition of A549 cells.
218). et al. Effect of Qizhu Granule on Liver Sinusoidal Endothelial Cells Capillarization in Carbon Tetrachloride-Induced Rats Fibrosis.
219). et al. PINK1 Contained in huMSCs-Exosomes Prevents Cardiomyocyte Mitochondrial Calcium Overload in Sepsis by Recovering Mitochondrial Ca2+ Efflux.
220). Liu YS;Yuan MH;Zhang CY;Liu HM;Liu JR;Wei AL;Ye Q;Zeng B;Li MF;Guo YP;Guo L; et al. Puerariae Lobatae radix flavonoids and puerarin alleviate alcoholic liver injury in zebrafish by regulating alcohol and lipid metabolism. Biomed Pharmacother 2020 Dec 17;134:111121. (PubMed: 33341668)
221). et al. Free CA125 promotes ovarian cancer cell migration and tumor metastasis by binding Mesothelin to reduce DKK1 expression and activate the SGK3/FOXO3 pathway.
222). et al. CircRNA_104565 Promoted Cell Proliferation in Papillary Thyroid Carcinoma by Sponging miR-134.
223). et al. Inhibition of NOX4 by Cissus quadrangularis extract protects from Type 2 diabetes induced-steatohepatitis.
224). et al. Estrogen deficiency exacerbates learning and memory deficits associated with glucose metabolism disorder in APP/PS1 double transgenic female mice.
225). et al. miRNA-92a-3p Regulates Osteoblast Differentiation in Patients with Concomitant Limb Fractures and Traumatic Brain Injury Through IBSP/PI3K-AKT Inhabitation.
226). et al. Hyperoside protects cardiomyocytes against hypoxia‑induced injury via upregulation of microRNA‑138.
227). et al. Protective effect of Wuling mycelia, alone and in combination with valproic acid, on pentylenetetrazolinduced epilepsy in rats.
228). et al. Regorafenib-Attenuated, Bleomycin-Induced Pulmonary Fibrosis by Inhibiting the TGF-β1 Signaling Pathway.
229). et al. Exosomal connexin 43 regulates the resistance of glioma cells to temozolomide.
230). et al. Chrysophanol localizes in mitochondria to promote cell death through upregulation of mitochondrial cyclophilin D in HepG2 cells.
231). et al. Gut microbiota-mediated transformation of coptisine into a novel metabolite 8-oxocoptisine: Insight into its superior anti-colitis effect.
232). Wang L;Yi S;Wang R;Wang J; et al. Long noncoding RNA KCNQ1OT1 promotes proliferation, migration, and invasion via targeting miR‐134 in retinoblastoma. J Gene Med 2021 Apr 5;e3336. (PubMed: 33818859)
233). et al. Evaluating the protective effects of individual or combined ginsenoside compound K and the downregulation of soluble epoxide hydrolase expression against sodium valproate-induced liver cell damage.
234). et al. Fractalkine Aggravates LPS-induced Macrophage Activation and Acute Kidney Injury via Wnt/β-catenin Signaling Pathway.
235). et al. MitoQ Inhibits Hepatic Stellate Cell Activation and Liver Fibrosis by Enhancing PINK1/parkin-mediated Mitophagy.
236). Li Y;Wu Q;Li L;Chen F;Bao J;Li W; et al. Decellularization of porcine whole lung to obtain a clinical‐scale bioengineered scaffold. J Biomed Mater Res A 2021 Mar 7. (PubMed: 33682365)
237). Sun Y;Lin J;Luo Z;Zhang Y;Chen J; et al. The Serum from Patients with Secondary Frozen Shoulder Following Rotator Cuff Repair Induces Shoulder Capsule Fibrosis and Promotes Macrophage Polarization and Fibroblast Activation. J Inflamm Res 2021 Mar 23;14:1055-1068. (PubMed: 33790620)
238). et al. Ultrafiltration Extract of Radix Angelica Sinensis and Radix Hedysari Attenuates Risk of Low-Dose X-Ray Radiation-Induced Myocardial Fibrosis In Vitro.
239). et al. DNA methyltransferase 1 inhibits O6-methylguanine-DNAmethyl-transferase-mediated cell growth and metastasis of hypopharyngeal squamous carcinoma.
240). Xun T;Lin Z;Wang X;Zhan X;Feng H;Gan D;Yang X; et al. Advanced oxidation protein products downregulate CYP1A2 and CYP3A4 expression and activity via the NF-κB-mediated signaling pathway in vitro and in vivo. Lab Invest 2021 May 24. (PubMed: 34031539)
241). et al. Targeting thymidine phosphorylase as a potential therapy for bone loss associated periprosthetic osteolysis.
242). et al. Cerebroprotein hydrolysate‐I protects senescence‐induced by D‐galactose in PC12 cells and mice.
243). et al. Comparative evaluation of methods for isolating small extracellular vesicles derived from pancreatic cancer cells.
244). et al. Long non‑coding RNA B3GALT5‑AS1 contributes to the progression of gastric cancer via interacting with CSNK2A1.
245). et al. Polydatin Protects Against Articular Cartilage Degeneration by Regulating Autophagy Mediated by AMPK/Mtor Signaling Pathway.
246). Sun T;Gong Q;Wu Y;Shen Z;Zhang Y;Ge S;Duan JS; et al. Dexmedetomidine alleviates cardiomyocyte apoptosis and cardiac dysfunction may be associated with inhibition of RhoA/ROCK pathway in mice with myocardial infarction. Naunyn Schmiedebergs Arch Pharmacol 2021 Jul;394(7):1569-1577. (PubMed: 33782744)
247). et al. Leonurine Ameliorates Oxidative Stress and Insufficient Angiogenesis by Regulating the PI3K/Akt-eNOS Signaling Pathway in H2O2-Induced HUVECs.
248). et al. FAM134B-Mediated ER-phagy Upregulation Attenuates AGEs-Induced Apoptosis and Senescence in Human Nucleus Pulposus Cells.
249). et al. Zanubrutinib Attenuated Bleomycin-Induced Pulmonary Fibrosis by Inhibiting the TGF-β1 Signaling Pathway.
250). et al. Abnormal N-glycosylated CPXM1 promotes gastric cancer metastasis through ITGB2/FAK pathway.
251). et al. Liquiritigenin Promotes Osteogenic Differentiation And Prevents Bone Loss Via Inducing Auto-lysosomal Degradation And Inhibiting Apoptosis.
252). et al. Intracellular pH-responsive iron-catechin nanoparticles with osteogenic/anti-adipogenic and immunomodulatory effects for efficient bone repair.
No comment
Total 0 records, divided into1 pages. First Prev Next Last

Submit Review

Support JPG, GIF, PNG format only
captcha
Catalog Number :

AF7021-BP
(Blocking peptide available as AF7021-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Horse
100%
Bovine
100%
Sheep
100%
Dog
100%
Chicken
100%
Rabbit
100%
Xenopus
90%
Zebrafish
0%
High similarity Medium similarity Low similarity No similarity
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

To Top