Home / Loading control & tag antibody / GAPDH Antibody

GAPDH Antibody AF7021

  • GAPDH Antibody
  • GAPDH Antibody
  • GAPDH Antibody
  • GAPDH Antibody
  • GAPDH Antibody
  • GAPDH Antibody
  • GAPDH Antibody
  • GAPDH Antibody
  • GAPDH Antibody
  • GAPDH Antibody
  • GAPDH Antibody
  • GAPDH Antibody
GAPDH Antibody
  • Catalog No.
    AF7021
  • Source
    Rabbit
  • P04406 UniProt
  • 2597 Gene ID
  • Predicted M.W.
    37kDa
  • Observed M.W.
    37kDa
  • Concentration
    1mg/ml
  • Clonality
    Polyclonal
  • Price/Size
    150$/50ul,250$/100ul,350$/200ul,1200$/1ml
  • Delivery time
    Same day delivery

Browsing history

Datasheet Validation(6) Citations(38) Review Blocking Peptides

1). Liu H et al. Hypoxia-inducible factor-1α promotes endometrial stromal cells migration and invasion by upregulating autophagy in endometriosis. Reproduction 2017 Jun;153(6):809-820 (PubMed: 28348069)

Application: WB(1/1000);   Species: human;    Sample:None Available;

WB


2). Xiang XJ et al. MiR-1271 Inhibits Cell Proliferation, Invasion and EMT in Gastric Cancer by Targeting FOXQ1. Cell Physiol Biochem 2015;36(4):1382-94 (PubMed: 26159618)

Application: WB;   Species: human;    Sample: human gastric cancer;

WB


3). Zhang J et al. Electrophysiological and trafficking defects of the SCN5A T353I mutation in Brugada syndrome are rescued by alpha-allocryptopine. Eur J Pharmacol 2015 Jan 5;746:333-43 (PubMed: 25261036)

Application: WB;   Species:None Available;    Sample:None Available;

WB


4). Sun H et al. Role of 5-HT1A receptor in insular cortex mediating stress - induced visceral sensory dysfunction. Neurogastroenterol Motil 2016 Jul;28(7):1104-13 (PubMed: 26969829)

Application: WB;   Species:None Available;    Sample:None Available;

WB


5). Liu X et al. The role of Delta-like 4 ligand/Notch-ephrin-B2 cascade in the pathogenesis of preeclampsia by regulating functions of endothelial progenitor cell. Placenta 2015 Sep;36(9):1002-10 (PubMed: 26212082)

Application: WB(1/2000);   Species: human;    Sample: human;

WB


6). X Niu et al. IL-17A exacerbates cisplatin-based resistance of OVCA via upregulating the expression of ABCG2 and MDR1 through Gli1-mediated Hh signaling. Oncotarget 2016 Jul 18; (PubMed: 27447857)

Application: None Available;   Species:None Available;    Sample:None Available;



7). Gao T et al. Aberrant hypomethylation and overexpression of the eyes absent homologue 2 suppresses tumor cell growth of human lung adenocarcinoma cells. Oncol Rep 2015 Nov;34(5):2333-42 (PubMed: 26329363)

Application: WB;   Species: human;    Sample: NSCLC cells;

WB


8). Zhao X et al. Up-regulation of miR-21 and miR-23a Contributes to As2 O3 -induced hERG Channel Deficiency. Basic Clin Pharmacol Toxicol 2015 Jun;116(6):516-23 (PubMed: 25395240)

Application: WB;   Species:None Available;    Sample:None Available;

WB


9). Fu WJ et al. Anti-atherosclerosis and cardio-protective effects of the Angong Niuhuang Pill on a high fat and vitamin D3 induced rodent model of atherosclerosis. J Ethnopharmacol 2017 Jan 4;195:118-126 (PubMed: 27880884)

Application: None Available;   Species:None Available;    Sample:None Available;



10). Liu W et al. dNK derived IFN-γ mediates VSMC migration and apoptosis via the induction of LncRNA MEG3: A role in uterovascular transformation. Placenta 2017 Feb;50:32-39 (PubMed: 28161059)

Application: None Available;   Species:None Available;    Sample:None Available;



11). Wang L et al. CDK3 is a major target of miR-150 in cell proliferation and anti-cancer effect. Exp Mol Pathol 2017 Apr;102(2):181-190 (PubMed: 28108217)

Application: WB(1/5000);   Species: human;    Sample: H1299 cells;

WB


12). Mao X et al. Inhibitors of PARP-1 exert inhibitory effects on the biological characteristics of hepatocellular carcinoma cells in vitro. Mol Med Rep 2017 Jul;16(1):208-214 (PubMed: 28498459)

Application: WB(1/1000);   Species: human;    Sample: HepG2;

WB


13). Liu X et al. Lipoxin A4 and its analog suppress inflammation by modulating HMGB1 translocation and expression in psoriasis. Sci Rep 2017 Aug 2;7(1):7100 (PubMed: 28769106)

Application: WB(1/1000);   Species: human;    Sample:None Available;

WB


14). Zhang J et al. Long non-coding RNA NEAT1 regulates E2F3 expression by competitively binding to miR-377 in non-small cell lung cancer. Oncol Lett 2017 Oct;14(4):4983-4988 (PubMed: 29085511)

Application: None Available;   Species:None Available;    Sample:None Available;



15). Liu YR et al. Twist1 confers multidrug resistance in colon cancer through upregulation of ATP-binding cassette transporters. Oncotarget 2017 May 2;8(32):52901-52912 (PubMed: 28881781)

Application: None Available;   Species:None Available;    Sample:None Available;



16). Pan L et al. The cardiac glycoside oleandrin induces apoptosis in human colon cancer cells via the mitochondrial pathway. Cancer Chemother Pharmacol 2017 Jul;80(1):91-100 (PubMed: 28597038)

Application: WB(1/5000);   Species: human;    Sample: SW480;

WB


17). Wang X et al. Autophagy enhanced antitumor effect in K562 and K562/ADM cells using realgar transforming solution. Biomed Pharmacother 2018 Feb;98:252-264 (PubMed: 29272786)

Application: None Available;   Species:None Available;    Sample:None Available;



18). Seo E et al. Exendin-4 in combination with adipose-derived stem cells promotes angiogenesis and improves diabetic wound healing. J Transl Med 2017 Feb 15;15(1):35 (PubMed: 28202074)

Application: None Available;   Species:None Available;    Sample:None Available;



19). Tian Y et al. Magnolol Alleviates Inflammatory Responses and Lipid Accumulation by AMP-Activated Protein Kinase-Dependent Peroxisome Proliferator-Activated Receptor α Activation. Front Immunol 2018 Feb 5;9:147 (PubMed: 29467759)

Application: None Available;   Species:None Available;    Sample:None Available;



20). Wu Y et al. Study on the mechanism of JAK2/STAT3 signaling pathway-mediated inflammatory reaction after cerebral ischemia. Mol Med Rep 2018 Apr;17(4):5007-5012 (PubMed: 29393445)

Application: None Available;   Species:None Available;    Sample:None Available;



21). Seo E et al. Exendin-4 in combination with adipose-derived stem cells promotes angiogenesis and improves diabetic wound healing. J Transl Med 2017 Feb 15;15(1):35 (PubMed: 28202074)

Application: None Available;   Species:None Available;    Sample:None Available;



22). Seo E et al. Exendin-4 in combination with adipose-derived stem cells promotes angiogenesis and improves diabetic wound healing. J Transl Med 2017 Feb 15;15(1):35 (PubMed: 28202074)

Application: None Available;   Species:None Available;    Sample:None Available;



23). Yuan Y et al. Regulation of Signaling Pathways Involved in the Anti-proliferative and Apoptosis-inducing Effects of M22 against Non-small Cell Lung Adenocarcinoma A549 Cells. Sci Rep 2018 Jan 17;8(1):992 (PubMed: 29343765)

Application: None Available;   Species:None Available;    Sample:None Available;



24). Seo E et al. Exendin-4 in combination with adipose-derived stem cells promotes angiogenesis and improves diabetic wound healing. J Transl Med 2017 Feb 15;15(1):35 (PubMed: 28202074)

Application: None Available;   Species:None Available;    Sample:None Available;



25). Meng J et al. Twist1 Regulates Vimentin through Cul2 Circular RNA to Promote EMT in Hepatocellular Carcinoma. Cancer Res 2018 Aug 1;78(15):4150-4162 (PubMed: 29844124)

Application: None Available;   Species:None Available;    Sample:None Available;



26). Li XH et al. Parthenolide attenuated bleomycin-induced pulmonary fibrosis via the NF-κB/Snail signaling pathway. Respir Res 2018 Jun 5;19(1):111 (PubMed: 29871641)

Application: None Available;   Species:None Available;    Sample:None Available;



27). Liu H et al. Autophagy contributes to hypoxia-induced epithelial to mesenchymal transition of endometrial epithelial cells in endometriosis. Biol Reprod 2018 May 31 (PubMed: 29860279)

Application: None Available;   Species:None Available;    Sample:None Available;



28). Hu J et al. Effects of Xin-Ji-Er-Kang on heart failure induced by myocardial infarction: Role of inflammation, oxidative stress and endothelial dysfunction. Phytomedicine 2018 Mar 15;42:245-257 (PubMed: 29655692)

Application: None Available;   Species:None Available;    Sample:None Available;



29). Seo E et al. Exendin-4 in combination with adipose-derived stem cells promotes angiogenesis and improves diabetic wound healing. J Transl Med 2017 Feb 15;15(1):35 (PubMed: 28202074)

Application: None Available;   Species:None Available;    Sample:None Available;



30). Ji S et al. Resveratrol promotes oxidative stress to drive DLC1 mediated cellular senescence in cancer cells. Exp Cell Res 2018 Sep 15;370(2):292-302 (PubMed: 29964052)

Application: None Available;   Species:None Available;    Sample:None Available;



31). Seo E et al. Exendin-4 in combination with adipose-derived stem cells promotes angiogenesis and improves diabetic wound healing. J Transl Med 2017 Feb 15;15(1):35 (PubMed: 28202074)

Application: None Available;   Species:None Available;    Sample:None Available;



32). Xu H et al. Involvement of insulin signalling pathway in methamphetamine-induced hyperphosphorylation of Tau. Toxicology 2018 Jul 4;408:88-94 (PubMed: 29981415)

Application: None Available;   Species:None Available;    Sample:None Available;



33). Meng J et al. Hsp90β promotes aggressive vasculogenic mimicry via epithelial-mesenchymal transition in hepatocellular carcinoma. Oncogene 2018 Aug 7 (PubMed: 30087438)

Application: None Available;   Species:None Available;    Sample:None Available;



34). Yun-Peng Shi et al. Synaptic plasticity of Human Umbilical Cord Mesenchymal Stem Cell differentiating into neuron like cells in vitro induced by Edavarone. STEM CELLS INT 2018 Aug;

Application: None Available;   Species:None Available;    Sample:None Available;



35). et al. Conditioned Medium from Human Umbilical Vein Endothelial Cells Promotes Proliferation, Migration, Invasion and Angiogenesis of Adipose Derived Stem Cells. (PubMed: 30074161)

Application: None Available;   Species:None Available;    Sample:None Available;



36). Sun C et al. Hsa-miR-326 targets CCND1 and inhibits non-small cell lung cancer development. Oncotarget 2016 Feb 16;7(7):8341-59 (PubMed: 26840018)

Application: WB(1/5000);   Species:None Available;    Sample:None Available;

WB


37). Zhan G et al. PGC-1α-FNDC5-BDNF signaling pathway in skeletal muscle confers resilience to stress in mice subjected to chronic social defeat. Psychopharmacology (Berl) 2018 Sep 26 (PubMed: 30259075)

Application: None Available;   Species:None Available;    Sample:None Available;



38). Zhou J et al. Adipose derived mesenchymal stem cells alleviated osteoarthritis and chondrocyte apoptosis through autophagy inducing. J Cell Biochem 2018 Oct 13 (PubMed: 30315711)

Application: None Available;   Species:None Available;    Sample:None Available;



Expand↓

Alternative Names:

GAPDH, A1 40 kd subunit, Activator 1 40 kd subunit, G3PD, GAPD, G3pdh, Rfc40, Rf-c 40 kd subunit

Applications:

WB 1:3000-1:30000 IHC 1:200 IF 1:200, ELISA(peptide) 1:20000-1:40000

Source:

Rabbit

Reactivity:

Human,Mouse,Rat,Pig,Zebrafish,Bovine,Goat,Monkey,Chicken

Clonality:

Polyclonal

Concentration:

1mg/ml

Molecular Weight:

Observed: 37kDa. Predicted: 37kDa.

GAPDH Antibody Western blot analysis of GAPDH expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Western blot analysis of GAPDH expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
GAPDH Antibody Western blot analysis of extracts of various sample,using GAPDH antibody. Western blot analysis of extracts of various sample,using GAPDH antibody.
GAPDH Antibody AF7021 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary. AF7021 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
GAPDH Antibody AF7021 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary. AF7021 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
GAPDH Antibody Western blot analysis of extracts from rat brain, mouse muscle, rat liver, using GAPDH antibody. Western blot analysis of extracts from rat brain, mouse muscle, rat liver, using GAPDH antibody.
GAPDH Antibody AF7021 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody. AF7021 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

GAPDH antibody detects endogenous levels of total GAPDH.

Immunogen:

A synthesized peptide derived from human GAPDH.

Uniprot:

Gene id:

Research Fields:

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.(View pathway)
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
· Metabolism > Global and overview maps > Biosynthesis of amino acids.
· Metabolism > Global and overview maps > Carbon metabolism.
· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.
· Metabolism > Global and overview maps > Metabolic pathways.

Description:

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) iswell known as one of the key enzymes involved in glycolysis.As well as functioning as a glycolytic enzyme in cytoplasm,recent evidence suggests that mammalian GAPDHis also involved in a great number of intracellular procesessuch as membrane fusion, microtubule bundling, phosphotransferaseactivity, nuclear RNA export, DNA replication,and DNA repair.

Function:

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

Subcellular Location:

Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.

Post-translational Modifications:

S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus (By similarity). S-nitrosylation of Cys-247 is induced by interferon-gamma and LDL(ox) implicating the iNOS-S100A8/9 transnitrosylase complex and seems to prevent interaction with phosphorylated RPL13A and to interfere with GAIT complex activity.ISGylated.Sulfhydration at Cys-152 increases catalytic activity.Oxidative stress can promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process called nucleocytoplasmic coagulation. Such aggregates can be observed in vivo in the affected tissues of patients with Alzheimer disease or alcoholic liver cirrhosis, or in cell cultures during necrosis. Oxidation at Met-46 may play a pivotal role in the formation of these insoluble structures. This modification has been detected in vitro following treatment with free radical donor (+/-)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide. It has been proposed to destabilize nearby residues, increasing the likelihood of secondary oxidative damages, including oxidation of Tyr-45 and Met-105. This cascade of oxidations may augment GAPDH misfolding, leading to intermolecular disulfide cross-linking and aggregation.

Subunit Structure:

Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with CHP1; the interaction increases the binding of CHP1 with microtubules. Associates with microtubules (By similarity). Interacts with EIF1AD, USP25, PRKCI and WARS. Interacts with phosphorylated RPL13A; inhibited by oxidatively-modified low-densitity lipoprotein (LDL(ox)). Component of the GAIT complex. Interacts with FKBP6; leading to inhibit GAPDH catalytic activity.

Similarity:

The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Protein BLAST With

Sequence:

        10         20         30         40         50
MGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD
60 70 80 90 100
STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG
110 120 130 140 150
VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA
160 170 180 190 200
SCTTNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR
210 220 230 240 250
GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE
260 270 280 290 300
KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG
310 320 330
IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKE

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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Catalog Number :

AF7021-BP

Price/Size :

$200/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

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