Price Size
$150 50ul
$250 100ul
$350 200ul
$1200 1ml

Same day delivery


For pricing and ordering contact:

local distributors
  • Product Name
    Tubulin gamma Antibody
  • Catalog No.
    AF7012
  • RRID
    AB_2839419
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC,ELISA(peptide)
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Zebrafish, Rabbit, Xenopus
  • UniProt
  • Mol.Wt
    51kD;
    51kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

Related Products

Product Information

Alternative Names:Expand▼

TUBG1, Gamma-1-tubulin, GCP-1, Gamma Tubulin, TUBGCP1, Tubulin gamma chain, TUBG, Tubulin, gamma polypeptide, Tubulin gamma-1 chain, Tubulin, gamma 1

Applications:

WB 1:000-1:5000, IHC 1:50-1:200, IF/ICC 1:200-500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Zebrafish, Rabbit, Xenopus

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

Tubulin gamma Antibody detects endogenous levels of total Tubulin gamma.

RRID:

AB_2839419
Please cite this product as: Affinity Biosciences Cat# AF7012, RRID:AB_2839419.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthesized peptide derived from human Tubulin gamma.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

TUBG1

Molecular Weight:

Observed Mol.Wt.: 51kD.
Predicted Mol.Wt.: 51kDa(Calculated)..

Subcellular Location:

Cytoplasm > cytoskeleton > centrosome.

Description:

TUBG1 Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. Interacts with GCP2 and GCP3. Interacts with B9D2. Interacts with CDK5RAP2;

Sequence:
MPREIITLQLGQCGNQIGFEFWKQLCAEHGISPEGIVEEFATEGTDRKDVFFYQADDEHYIPRAVLLDLEPRVIHSILNSPYAKLYNPENIYLSEHGGGAGNNWASGFSQGEKIHEDIFDIIDREADGSDSLEGFVLCHSIAGGTGSGLGSYLLERLNDRYPKKLVQTYSVFPNQDEMSDVVVQPYNSLLTLKRLTQNADCVVVLDNTALNRIATDRLHIQNPSFSQINQLVSTIMSASTTTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQSVASVRKTTVLDVMRRLLQPKNVMVSTGRDRQTNHCYIAILNIIQGEVDPTQVHKSLQRIRERKLANFIPWGPASIQVALSRKSPYLPSAHRVSGLMMANHTSISSLFERTCRQYDKLRKREAFLEQFRKEDMFKDNFDEMDTSREIVQQLIDEYHAATRPDYISWGTQEQ

Research Background

Function:

Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.

Post-translational Modifications:

Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

Interacts with TUBGCP2 and TUBGCP3. Interacts with B9D2 (By similarity). Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2. Interacts with PIFO. Interacts with SAS6 and NUP62 at the centrosome.

Similarity:

Belongs to the tubulin family.

Research Fields

Research Fields:

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

No comment
Total 0 records, divided into1 pages. First Prev Next Last

Submit Review

Support JPG, GIF, PNG format only
captcha
Catalog Number :

AF7012-BP
(Blocking peptide available as AF7012-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Rabbit
87%
Zebrafish
87%
Xenopus
80%
Pig
73%
Horse
73%
Bovine
73%
Sheep
73%
Dog
73%
Chicken
0%
High similarity Medium similarity Low similarity No similarity
P23258 as Substrate
Site PTM Type Enzyme
S32 Phosphorylation
K48 Ubiquitination
S80 Phosphorylation P24941 (CDK2)
K84 Ubiquitination
K113 Ubiquitination
S129 Phosphorylation
S131 Phosphorylation Q8TDC3-2 (BRSK1)
K164 Ubiquitination
K193 Ubiquitination
S284 Phosphorylation
K287 Ubiquitination
R295 Methylation
K301 Ubiquitination
S306 Phosphorylation
T307 Phosphorylation
K335 Ubiquitination
S336 Phosphorylation
K344 Ubiquitination
K363 Ubiquitination
S364 Phosphorylation
S385 Phosphorylation Q8TDC3 (BRSK1)
K397 Acetylation
K400 Acetylation
K400 Ubiquitination
K410 Ubiquitination
K415 Ubiquitination
T423 Phosphorylation
S424 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

To Top