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  • Product Name
    Tubulin alpha Antibody
  • Catalog No.
    AF7010
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC,ELISA
  • Reactivity
    Hm,Ms,Rt,Pg,Bv,Rb,Ck,Fs
  • UniProt
  • Mol.Wt.
    50kDa
  • Concentration
    1mg/ml
  • Browse similar products>>

Product Information

Alternative Names:Expand▼

TUBA1A; Alpha-tubulin 3; B-ALPHA-1; Hum-a-tub1; TUBA3; Tubulin alpha-3 chain; Tubulin B-alpha-1; Tubulin, alpha 1a; Tubulin; alpha; brain-specific; Hum-a-tub2; LIS3; Tubulin Alpha 1a; Tubulin alpha-1A chain;

Applications:

WB 1:5000-1:20000 IHC 1:50-1:200 IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Reactivity:

Human,Mouse,Rat,Pig,Bovine,Rabbit,Chicken,Fish

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

Tubulin alpha Antibody detects endogenous levels of total Tubulin alpha.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human Tubulin alpha.

Uniprot:



>>Visit The Human Protein Atlas

Gene id:

Molecular Weight:

Observed Mol.Wt.: 50kDa.
Predicted Mol.Wt.: 51kDa.

Subcellular Location:

Cytoplasm, cytoskeleton.

Description:

TUBA1B Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Belongs to the tubulin family

Sequence:
        10         20         30         40         50
MRECISIHVG QAGVQIGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDSFN
60 70 80 90 100
TFFSETGAGK HVPRAVFVDL EPTVIDEVRT GTYRQLFHPE QLITGKEDAA
110 120 130 140 150
NNYARGHYTI GKEIIDLVLD RIRKLADQCT GLQGFLVFHS FGGGTGSGFT
160 170 180 190 200
SLLMERLSVD YGKKSKLEFS IYPAPQVSTA VVEPYNSILT THTTLEHSDC
210 220 230 240 250
AFMVDNEAIY DICRRNLDIE RPTYTNLNRL ISQIVSSITA SLRFDGALNV
260 270 280 290 300
DLTEFQTNLV PYPRIHFPLA TYAPVISAEK AYHEQLSVAE ITNACFEPAN
310 320 330 340 350
QMVKCDPRHG KYMACCLLYR GDVVPKDVNA AIATIKTKRS IQFVDWCPTG
360 370 380 390 400
FKVGINYQPP TVVPGGDLAK VQRAVCMLSN TTAIAEAWAR LDHKFDLMYA
410 420 430 440 450
KRAFVHWYVG EGMEEGEFSE AREDMAALEK DYEEVGVDSV EGEGEEEGEE

Y

Background

Function:

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Post-translational Modifications:

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly.Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability.Nitration of Tyr-451 is irreversible and interferes with normal dynein intracellular distribution.Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.Tubulin alpha-1B chain: Tyrosination promotes microtubule interaction with CAP-Gly domain-containing proteins such as CLIP1, CLIP2 and DCTN1 (By similarity). Tyrosination regulates the initiation of dynein-dynactin motility via interaction with DCTN1, which brings the dynein-dynactin complex into contact with microtubules (PubMed:26972003). In neurons, tyrosinated tubulins mediate the initiation of retrograde vesicle transport (By similarity).Detyrosinated tubulin alpha-1B chain: Detyrosination is involved in metaphase plate congression by guiding chromosomes during mitosis: detyrosination promotes interaction with CENPE, promoting pole-proximal transport of chromosomes toward the equator (PubMed:25908662). Detyrosination increases microtubules-dependent mechanotransduction in dystrophic cardiac and skeletal muscle. In cardiomyocytes, detyrosinated microtubules are required to resist to contractile compression during contraction: detyrosination promotes association with desmin (DES) at force-generating sarcomeres, leading to buckled microtubules and mechanical resistance to contraction (By similarity).

Subcellular Location:

Cytoskeleton;

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.

Similarity:

Belongs to the tubulin family.

Research Fields

Research Fields:

· Cellular Processes > Transport and catabolism > Phagosome.(View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction.(View pathway)
· Cellular Processes > Cellular community - eukaryotes > Gap junction.(View pathway)
· Cellular Processes > Cell growth and death > Apoptosis.(View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

Western blot analysis of Tubulin alpha expression in various sample
Acetylated α-Tubulin Regulated by N-Acetyl-Seryl-Aspartyl-Lysyl-Proline (Ac-SDKP) Exerts the Anti-fibrotic Effect in Rat Lung Fibrosis Induced by Silica W Xiaojun, L Yan, X Hong, Z Xianghong… Scientific …, 2016 ncbi.nlm.nih.gov
Western blot analysis of Tubulin alpha expression in various sample
Western blot analysis of Tubulin alpha expression in various whole cell lysates
AF7010 at 1/200 staining human colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF7010 at 1/100 staining human breast tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at106
AF7010 at 1/200 staining human colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF7010 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Acetylated α-Tubulin Regulated by N-Acetyl-Seryl-Aspartyl-Lysyl-Proline (Ac-SDKP) Exerts the Anti-fibrotic Effect in Rat Lung Fibrosis Induced by Silica W Xiaojun, L Yan, X Hong, Z Xianghong… Scientific …, 2016 ncbi.nlm.nih.gov

Reference Citations:

1). Qiu P et al. Herbal SGR Formula Prevents Acute Ethanol-Induced Liver Steatosis via Inhibition of Lipogenesis and Enhancement Fatty Acid Oxidation in Mice. Evid Based Complement Alternat Med 2015;2015:613584 (PubMed: 26101535)

Application: WB    Species: mouse;    Sample: mouse;


2). Xiaojun W et al. Acetylated α-Tubulin Regulated by N-Acetyl-Seryl-Aspartyl-Lysyl-Proline(Ac-SDKP) Exerts the Anti-fibrotic Effect in Rat Lung Fibrosis Induced by Silica. Sci Rep 2016 Aug 31;6:32257 (PubMed: 27577858)

Application: WB    Species: rat;    Sample:Not available;

Figure 3. Effect of Ac-SDKP on col I, α-SMA, and α-Ac-Tub in silicosis rats. (a) The expression of α-SMA and α-Ac-Tub in lung tissue measured by immunohistochemistry. Scale bar=200μm and 50μm. (b) The expression of col I, α-SMA and α-Ac-Tub in lung tissue measured by Western blot. Data presented as mean±SEM; N=4 independent experiments.

Application: IF/ICC    Species: rat;    Sample:Not available;

Figure 2. The co-expression of Tub-α and α-Ac-Tub in lung tissue and fibroblasts. (a) The co-expression of Tub-α and α-Ac-Tub in lungs of rat silicosis model and fibroblasts induced by Ang II measured by immunofluorescence. Scale bar=100μm and 50μm


3). Li H et al. Increased TMEM16A Involved in Alveolar Fluid Clearance After Lipopolysaccharide Stimulation. Inflammation 2016 Apr;39(2):881-90 (PubMed: 26899569)

Application: IF/ICC    Species: mouse;    Sample: lung tissue;

Fig. 3. The effects of LPS on TMEM16A protein expression in LA795 cells and lung tissue. a Plasma membrane localization of endogenous TMEM16A in LA795 cells. Cells were stained with anti-α-tubulin (green) and anti-TMEM16A antibodies (red). Nuclei were stained with Hoechst (blue). TMEM16A expression was significantly increased by LPS stimulation (10 μg/ml, 24 h). a1–d1 control; a2–d2 LPS (10 μg/ml). The bars represent 20 μm. TMEMpositive cell numbers per image field in the immunofluorescence staining were calculated


4). He J et al. PTEN Reduced UVB-Mediated Apoptosis in Retinal Pigment Epithelium Cells. Biomed Res Int 2017;2017:3681707 (PubMed: 28321407)

Application: WB    Species: human;    Sample:Not available;

????-Tubulin expression was detected as the loading control


5). Liu L et al. The inhibition of NOTCH2 reduces UVB-induced damage in retinal pigment epithelium cells. Mol Med Rep 2017 Jul;16(1):730-736 (PubMed: 28560393)

6). Zhang X et al. Inhibition of transmembrane member 16A calcium-activated chloride channels by natural flavonoids contributes to flavonoid anticancer effects. Br J Pharmacol 2017 Jul;174(14):2334-2345 (PubMed: 28452066)

7). Liu H et al. Protective effect of lutein on ARPE-19 cells upon H2O2-induced G2/M arrest. Mol Med Rep 2017 Aug;16(2):2069-2074 (PubMed: 28656238)

8). Qiu P et al. Dihydromyricetin modulates p62 and autophagy crosstalk with the Keap-1/Nrf2 pathway to alleviate ethanol-induced hepatic injury. Toxicol Lett 2017 May 15;274:31-41 (PubMed: 28419832)

Application: WB    Species:Not available;    Sample:Not available;


9). Huang Z et al. Notch signaling pathway mediates Doxorubicin-driven apoptosis in cancers. Cancer Manag Res 2018 Jun 8;10:1439-1448 (PubMed: 29922088)

10). et al. Proteomic Profile of TGF-β1 treated Lung Fibroblasts identifies Novel Markers of Activated Fibroblasts in the Silica Exposed Rat Lung.

11). et al. Semen hoveniae extract ameliorates alcohol-induced chronic liver damage in rats via modulation of the abnormalities of Gut-Liver Axis.

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Catalog Number :

AF7010-BP

Price/Size :

$200/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.