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$150 50ul
$250 100ul
$350 200ul
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  • Product Name
    Tubulin alpha Antibody
  • Catalog No.
    AF7010
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC,ELISA
  • Reactivity:
    Human, Mouse, Rat, Pig, Bovine, Rabbit, Chicken, Fish
  • Prediction:
    Horse(100%), Sheep(100%), Dog(100%)
  • UniProt
  • Mol.Wt.
    50kDa
  • Concentration
    1mg/ml
  • Browse similar products>>

Product Information

Alternative Names:Expand▼

TUBA1A; Alpha-tubulin 3; B-ALPHA-1; Hum-a-tub1; TUBA3; Tubulin alpha-3 chain; Tubulin B-alpha-1; Tubulin, alpha 1a; Tubulin; alpha; brain-specific; Hum-a-tub2; LIS3; Tubulin Alpha 1a; Tubulin alpha-1A chain;

Applications:

WB 1:5000-1:20000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Reactivity:

Human, Mouse, Rat, Pig, Bovine, Rabbit, Chicken, Fish

Predicted Reactivity:

Horse(100%), Sheep(100%), Dog(100%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

Tubulin alpha Antibody detects endogenous levels of total Tubulin alpha.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human Tubulin alpha.

Uniprot:



>>Visit The Human Protein Atlas

Gene id:

Molecular Weight:

Observed Mol.Wt.: 50kDa.
Predicted Mol.Wt.: 51kDa.

Subcellular Location:

Cytoplasm, cytoskeleton.

Description:

TUBA1B Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Belongs to the tubulin family

Sequence:
        10         20         30         40         50
MRECISIHVG QAGVQIGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDSFN
60 70 80 90 100
TFFSETGAGK HVPRAVFVDL EPTVIDEVRT GTYRQLFHPE QLITGKEDAA
110 120 130 140 150
NNYARGHYTI GKEIIDLVLD RIRKLADQCT GLQGFLVFHS FGGGTGSGFT
160 170 180 190 200
SLLMERLSVD YGKKSKLEFS IYPAPQVSTA VVEPYNSILT THTTLEHSDC
210 220 230 240 250
AFMVDNEAIY DICRRNLDIE RPTYTNLNRL ISQIVSSITA SLRFDGALNV
260 270 280 290 300
DLTEFQTNLV PYPRIHFPLA TYAPVISAEK AYHEQLSVAE ITNACFEPAN
310 320 330 340 350
QMVKCDPRHG KYMACCLLYR GDVVPKDVNA AIATIKTKRS IQFVDWCPTG
360 370 380 390 400
FKVGINYQPP TVVPGGDLAK VQRAVCMLSN TTAIAEAWAR LDHKFDLMYA
410 420 430 440 450
KRAFVHWYVG EGMEEGEFSE AREDMAALEK DYEEVGVDSV EGEGEEEGEE

Y

Background

Function:

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Post-translational Modifications:

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly.Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability.Nitration of Tyr-451 is irreversible and interferes with normal dynein intracellular distribution.Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.Tubulin alpha-1B chain: Tyrosination promotes microtubule interaction with CAP-Gly domain-containing proteins such as CLIP1, CLIP2 and DCTN1 (By similarity). Tyrosination regulates the initiation of dynein-dynactin motility via interaction with DCTN1, which brings the dynein-dynactin complex into contact with microtubules (PubMed:26972003). In neurons, tyrosinated tubulins mediate the initiation of retrograde vesicle transport (By similarity).Detyrosinated tubulin alpha-1B chain: Detyrosination is involved in metaphase plate congression by guiding chromosomes during mitosis: detyrosination promotes interaction with CENPE, promoting pole-proximal transport of chromosomes toward the equator (PubMed:25908662). Detyrosination increases microtubules-dependent mechanotransduction in dystrophic cardiac and skeletal muscle. In cardiomyocytes, detyrosinated microtubules are required to resist to contractile compression during contraction: detyrosination promotes association with desmin (DES) at force-generating sarcomeres, leading to buckled microtubules and mechanical resistance to contraction (By similarity).

Subcellular Location:

Cytoskeleton;

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.

Similarity:

Belongs to the tubulin family.

Research Fields

Research Fields:

· Cellular Processes > Transport and catabolism > Phagosome.(View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction.(View pathway)
· Cellular Processes > Cellular community - eukaryotes > Gap junction.(View pathway)
· Cellular Processes > Cell growth and death > Apoptosis.(View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

Western blot analysis of Tubulin alpha expression in various sample
Western blot analysis of Tubulin alpha expression in various sample
AF7010 at 1/100 staining human breast tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at106
AF7010 at 1/200 staining human colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF7010 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AF7010 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.

Reference Citations:

1). Liu K et al. Melatonin reduces intramuscular fat deposition by promoting lipolysis and increasing mitochondrial function. J Lipid Res 2018 Dec 14 (PubMed: 30552289) [IF=4.743]

2). Sun R et al. Rat sinus mucosa- and periosteum-derived exosomes accelerate osteogenesis. J Cell Physiol 2019 May 9 (PubMed: 31074002) [IF=4.522]

3). Qiu P et al. Semen hoveniae extract ameliorates alcohol-induced chronic liver damage in rats via modulation of the abnormalities of gut-liver axis. Phytomedicine 2019 Jan;52:40-50 (PubMed: 30599911) [IF=4.180]

4). Xiaojun W et al. Acetylated α-Tubulin Regulated by N-Acetyl-Seryl-Aspartyl-Lysyl-Proline(Ac-SDKP) Exerts the Anti-fibrotic Effect in Rat Lung Fibrosis Induced by Silica. Sci Rep 2016 Aug 31;6:32257 (PubMed: 27577858) [IF=4.011]

Application: WB    Species:rat;    Sample:Not available

Figure 3. Effect of Ac-SDKP on col I, α-SMA, and α-Ac-Tub in silicosis rats. (a) The expression of α-SMA and α-Ac-Tub in lung tissue measured by immunohistochemistry. Scale bar=200μm and 50μm. (b) The expression of col I, α-SMA and α-Ac-Tub in lung tissue measured by Western blot. Data presented as mean±SEM; N=4 independent experiments.

Application: IF/ICC    Species:rat;    Sample:Not available

Figure 2. The co-expression of Tub-α and α-Ac-Tub in lung tissue and fibroblasts. (a) The co-expression of Tub-α and α-Ac-Tub in lungs of rat silicosis model and fibroblasts induced by Ang II measured by immunofluorescence. Scale bar=100μm and 50μm


5). Liu H et al. Protective effect of lutein on ARPE-19 cells upon H2O2-induced G2/M arrest. Mol Med Rep 2017 Aug;16(2):2069-2074 (PubMed: 28656238)

6). Liu L et al. The inhibition of NOTCH2 reduces UVB-induced damage in retinal pigment epithelium cells. Mol Med Rep 2017 Jul;16(1):730-736 (PubMed: 28560393)

7). Na Mao et al. Proteomic Profile of TGF-β1 treated Lung Fibroblasts identifies Novel Markers of Activated Fibroblasts in the Silica Exposed Rat Lung. bioRxiv 2018

8). Wang YP et al. Chronic photodamage in the chicken retina using 650-nm semiconductor laser. Int J Ophthalmol 2019 Jun 18;12(6):936-942 (PubMed: 31236349)

9). Li H et al. Increased TMEM16A Involved in Alveolar Fluid Clearance After Lipopolysaccharide Stimulation. Inflammation 2016 Apr;39(2):881-90 (PubMed: 26899569)

Application: IF/ICC    Species:mouse;    Sample:lung tissue

Fig. 3. The effects of LPS on TMEM16A protein expression in LA795 cells and lung tissue. a Plasma membrane localization of endogenous TMEM16A in LA795 cells. Cells were stained with anti-α-tubulin (green) and anti-TMEM16A antibodies (red). Nuclei were stained with Hoechst (blue). TMEM16A expression was significantly increased by LPS stimulation (10 μg/ml, 24 h). a1–d1 control; a2–d2 LPS (10 μg/ml). The bars represent 20 μm. TMEMpositive cell numbers per image field in the immunofluorescence staining were calculated


10). Qiu P et al. Dihydromyricetin modulates p62 and autophagy crosstalk with the Keap-1/Nrf2 pathway to alleviate ethanol-induced hepatic injury. Toxicol Lett 2017 May 15;274:31-41 (PubMed: 28419832)

Application: WB    Species:Not available;    Sample:Not available


11). Wei Z et al. Rho GDP dissociation inhibitor α silencing attenuates silicosis by inhibiting RhoA/Rho kinase signalling. Exp Cell Res 2019 Apr 25 (PubMed: 31029634)

12). Qiu P et al. Herbal SGR Formula Prevents Acute Ethanol-Induced Liver Steatosis via Inhibition of Lipogenesis and Enhancement Fatty Acid Oxidation in Mice. Evid Based Complement Alternat Med 2015;2015:613584 (PubMed: 26101535)

Application: WB    Species:mouse;    Sample:mouse


13). Zhang X et al. Inhibition of transmembrane member 16A calcium-activated chloride channels by natural flavonoids contributes to flavonoid anticancer effects. Br J Pharmacol 2017 Jul;174(14):2334-2345 (PubMed: 28452066)

14). He J et al. PTEN Reduced UVB-Mediated Apoptosis in Retinal Pigment Epithelium Cells. Biomed Res Int 2017;2017:3681707 (PubMed: 28321407)

Application: WB    Species:human;    Sample:Not available

????-Tubulin expression was detected as the loading control


15). Huang Z et al. Notch signaling pathway mediates Doxorubicin-driven apoptosis in cancers. Cancer Manag Res 2018 Jun 8;10:1439-1448 (PubMed: 29922088)

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Catalog Number :

AF7010-BP

Price/Size :

$200/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Sheep
100%
Dog
100%
Bovine
100%
Horse
100%
Rabbit
0%
Chicken
0%
Xenopus
0%
Zebrafish
0%
High similarity Medium similarity Low similarity No similarity
P68363/Q71U36 as Substrate
Site PTM Type Enzyme
S6 Phosphorylation
Y24 Phosphorylation
S38 Phosphorylation
K40 Acetylation
K40 Methylation
K40 Ubiquitination
T41 Phosphorylation
S48 Phosphorylation
T51 Phosphorylation
S54 Phosphorylation
T56 Phosphorylation
K60 Acetylation
K60 Sumoylation
K60 Ubiquitination
T73 Phosphorylation
T80 Phosphorylation
T82 Phosphorylation
Y83 Phosphorylation
T94 Phosphorylation
K96 Acetylation
K96 Sumoylation
K96 Ubiquitination
Y103 Phosphorylation
Y108 Phosphorylation
T109 Phosphorylation
K112 Acetylation
K112 Sumoylation
K112 Ubiquitination
K124 Acetylation
K124 Ubiquitination
S151 Phosphorylation
S158 Phosphorylation
Y161 Phosphorylation
K163 Acetylation
K163 Methylation
K163 Ubiquitination
K164 Ubiquitination
S165 Phosphorylation
K166 Ubiquitination
Y172 Phosphorylation
Y185 Phosphorylation
S187 Phosphorylation
T190 Phosphorylation
T191 Phosphorylation
T193 Phosphorylation
T194 Phosphorylation
S198 Phosphorylation
Y210 Phosphorylation
T223 Phosphorylation
Y224 Phosphorylation
T225 Phosphorylation
S236 Phosphorylation
S237 Phosphorylation
T253 Phosphorylation
T257 Phosphorylation
Y262 Phosphorylation
T271 Phosphorylation
Y272 Phosphorylation
S277 Phosphorylation
K280 Ubiquitination
Y282 Phosphorylation
S287 Phosphorylation
T292 Phosphorylation
K304 Ubiquitination
K311 Acetylation
K311 Ubiquitination
Y312 Phosphorylation
Y319 Phosphorylation
K326 Acetylation
K326 Sumoylation
K326 Ubiquitination
T334 Phosphorylation
K336 Acetylation
K336 Sumoylation
K336 Ubiquitination
T337 Phosphorylation
K338 Methylation
K338 Ubiquitination
T340 Phosphorylation
T349 Phosphorylation
K352 Acetylation
K352 Sumoylation
K352 Ubiquitination
Y357 Phosphorylation
T361 Phosphorylation
K370 Acetylation
K370 Sumoylation
K370 Ubiquitination
S379 Phosphorylation
K394 Acetylation
K394 Sumoylation
K394 Ubiquitination
Y399 Phosphorylation
K401 Acetylation
K401 Sumoylation
K401 Ubiquitination
Y408 Phosphorylation
S419 Phosphorylation
K430 Ubiquitination
Y432 Phosphorylation
S439 Phosphorylation
Y451 Phosphorylation
Site PTM Type Enzyme
S6 Phosphorylation
Y24 Phosphorylation
S38 Phosphorylation
K40 Acetylation
K40 Methylation
K40 Ubiquitination
T41 Phosphorylation
S48 Phosphorylation
T51 Phosphorylation
S54 Phosphorylation
T56 Phosphorylation
K60 Acetylation
K60 Sumoylation
K60 Ubiquitination
T73 Phosphorylation
T80 Phosphorylation
T82 Phosphorylation
Y83 Phosphorylation
T94 Phosphorylation
K96 Acetylation
K96 Sumoylation
K96 Ubiquitination
Y103 Phosphorylation
Y108 Phosphorylation
T109 Phosphorylation
K112 Acetylation
K112 Sumoylation
K112 Ubiquitination
K124 Acetylation
K124 Ubiquitination
S151 Phosphorylation
S158 Phosphorylation
Y161 Phosphorylation
K163 Acetylation
K163 Methylation
K163 Ubiquitination
K164 Ubiquitination
K166 Ubiquitination
Y172 Phosphorylation
Y185 Phosphorylation
S187 Phosphorylation
T190 Phosphorylation
T191 Phosphorylation
T193 Phosphorylation
T194 Phosphorylation
S198 Phosphorylation
Y210 Phosphorylation
T223 Phosphorylation
Y224 Phosphorylation
T225 Phosphorylation
S232 Phosphorylation
S236 Phosphorylation
S237 Phosphorylation
T239 Phosphorylation
S241 Phosphorylation
T253 Phosphorylation
T257 Phosphorylation
Y262 Phosphorylation
T271 Phosphorylation
Y272 Phosphorylation
S277 Phosphorylation
K280 Acetylation
K280 Ubiquitination
Y282 Phosphorylation
S287 Phosphorylation
T292 Phosphorylation
C295 S-Nitrosylation
K304 Ubiquitination
K311 Acetylation
K311 Ubiquitination
Y312 Phosphorylation
C315 S-Nitrosylation
C316 S-Nitrosylation
Y319 Phosphorylation
K326 Acetylation
K326 Sumoylation
K326 Ubiquitination
T334 Phosphorylation
K336 Acetylation
K336 Sumoylation
K336 Ubiquitination
T337 Phosphorylation
K338 Methylation
K338 Ubiquitination
R339 Methylation
S340 Phosphorylation
C347 S-Nitrosylation
T349 Phosphorylation
K352 Acetylation
K352 Sumoylation
K352 Ubiquitination
Y357 Phosphorylation
T361 Phosphorylation
K370 Acetylation
K370 Sumoylation
K370 Ubiquitination
C376 S-Nitrosylation
S379 Phosphorylation
K394 Acetylation
K394 Methylation
K394 Sumoylation
K394 Ubiquitination
Y399 Phosphorylation
K401 Acetylation
K401 Sumoylation
K401 Ubiquitination
Y408 Phosphorylation
S419 Phosphorylation
K430 Ubiquitination
Y432 Phosphorylation
S439 Phosphorylation
Y451 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.