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beta-Actin Mouse Monoclonal Antibody T0022

  • beta-Actin Mouse Monoclonal Antibody
  • beta-Actin Mouse Monoclonal Antibody
  • beta-Actin Mouse Monoclonal Antibody
  • beta-Actin Mouse Monoclonal Antibody
beta-Actin Mouse Monoclonal Antibody
  • Catalog No.
    T0022
  • Source
    Mouse
  • P60709 UniProt
  • 60 Gene ID
  • Predicted M.W.
    42kDa
  • Observed M.W.
    43KD
  • Concentration
    1mg/ml
  • Clonality
    Monoclonal (F929)
  • Price/Size
    1200$/1ml,350$/200ul,250$/100ul,150$/50ul
  • Delivery time
    Same day delivery

Browsing history

Datasheet Validation(2) Citations(18) Review

1). Xiang XJ et al. MiR-1271 Inhibits Cell Proliferation, Invasion and EMT in Gastric Cancer by Targeting FOXQ1. Cell Physiol Biochem 2015;36(4):1382-94 (PubMed: 26159618)

Application: WB;   Species: human;    Sample: human gastric cancer;

WB


2). Zhang J et al. FOXQ1 promotes gastric cancer metastasis through upregulation of Snail. Oncol Rep 2016 Jun;35(6):3607-13 (PubMed: 27109028)

Application: WB;   Species: human;    Sample: BGC-823 cell;

WB


3). Wang Z et al. The role of MAPK signaling pathway in the Her-2-positive meningiomas. Oncol Rep 2016 Aug;36(2):685-95 (PubMed: 27279438)

Application: WB;   Species:None Available;    Sample:None Available;

WB


4). Yi S et al. Tcf12, A Member of Basic Helix-Loop-Helix Transcription Factors, Mediates Bone Marrow Mesenchymal Stem Cell Osteogenic Differentiation In Vitro and In Vivo. Stem Cells 2017 Feb;35(2):386-397 (PubMed: 27574032)

Application: WB;   Species:None Available;    Sample:None Available;

WB


5). Feng Y et al. Resveratrol attenuates neuronal autophagy and inflammatory injury by inhibiting the TLR4/NF-κB signaling pathway in experimental traumatic brain injury. Int J Mol Med 2016 Apr;37(4):921-30 (PubMed: 26936125)

Application: WB;   Species: rat;    Sample:None Available;

WB


6). Liang X et al. Rictor regulates the vasculogenic mimicry of melanoma via the AKT-MMP-2/9 pathway. J Cell Mol Med 2017 Dec;21(12):3579-3591 (PubMed: 28699701)

Application: WB;   Species: human;    Sample:None Available;

WB


7). Yang YF et al. Ficolin-A/2, acting as a new regulator of macrophage polarization, mediates the inflammatory response in experimental mouse colitis. Immunology 2017 Aug;151(4):433-450 (PubMed: 28380665)

Application: WB;   Species: mouse;    Sample:None Available;

WB


8). Zhou P et al. Combination therapy of PKCζ and COX-2 inhibitors synergistically suppress melanoma metastasis. J Exp Clin Cancer Res 2017 Sep 2;36(1):115 (PubMed: 28865485)

Application: WB;   Species: mouse;    Sample: B16F10;

WB


9). Guo J et al. Tumor-associated macrophages induce the expression of FOXQ1 to promote epithelial-mesenchymal transition and metastasis in gastric cancer cells. Oncol Rep 2017 Oct;38(4):2003-2010 (PubMed: 28791370)

Application: WB;   Species: human;    Sample:None Available;

WB


10). Yi S et al. Tcf12, A Member of Basic Helix-Loop-Helix Transcription Factors, Mediates Bone Marrow Mesenchymal Stem Cell Osteogenic Differentiation In Vitro and In Vivo. Stem Cells 2017 Feb;35(2):386-397 (PubMed: 27574032)

Application: None Available;   Species:None Available;    Sample:None Available;



11). Zhang W et al. Down-regulating Myoferlin inhibits the vasculogenic mimicry of melanoma via decreasing MMP-2 and inducing mesenchymal-to-epithelial transition. J Cell Mol Med 2018 Mar;22(3):1743-1754 (PubMed: 29164766)

Application: None Available;   Species:None Available;    Sample:None Available;



12). Wu L et al. KIF17 mediates the learning and memory impairment in offspring induced by maternal exposure to propofol during middle pregnancy. Mol Med Rep 2018 Apr;17(4):5428-5434 (PubMed: 29393422)

Application: None Available;   Species:None Available;    Sample:None Available;



13). Shao Q et al. MicroRNA-139-5p affects cisplatin sensitivity in human nasopharyngeal carcinoma cells by regulating the epithelial-to-mesenchymal transition. Gene 2018 Apr 30;652:48-58 (PubMed: 29427737)

Application: None Available;   Species:None Available;    Sample:None Available;



14). Yuan F et al. Leptin Signaling in the Carotid Body Regulates a Hypoxic Ventilatory Response Through Altering TASK Channel Expression. Front Physiol 2018 Mar 27;9:249 (PubMed: 29636698)

Application: None Available;   Species:None Available;    Sample:None Available;



15). Duan Z et al. Endothelin-1-induced expression of α-smooth muscle actin in human myometrial fibroblasts. J Obstet Gynaecol Res 2018 Mar;44(3):540-546 (PubMed: 29271089)

Application: None Available;   Species:None Available;    Sample:None Available;



16). Wang C et al. Cytoprotective effects of diosmetin against hydrogen peroxide-induced L02 cell oxidative damage via activation of the Nrf2-ARE signaling pathway. Mol Med Rep 2018 May;17(5):7331-7338 (PubMed: 29568961)

Application: None Available;   Species:None Available;    Sample:None Available;



17). Xia G et al. ROS‑mediated autophagy through the AMPK signaling pathway protects INS‑1 cells from human islet amyloid polypeptide‑induced cytotoxicity. Mol Med Rep 2018 Sep;18(3):2744-2752 (PubMed: 30015901)

Application: None Available;   Species:None Available;    Sample:None Available;



18). Feng Y et al. Neuroprotective Effects of Resatorvid Against Traumatic Brain Injury in Rat: Involvement of Neuronal Autophagy and TLR4 Signaling Pathway. Cell Mol Neurobiol 2017 Jan;37(1):155-168 (PubMed: 26961544)

Application: WB(1/1000);   Species: rat;    Sample: rat hippocampus;

WB


Expand↓

Alternative Names:

ACTB;Actin; cytoplasmic 1; Beta-actin; Beta actin; BRWS1; Actin; beta; Beta cytoskeletal actin; PS1TP5-binding protein 1; PS1TP5BP1;

Applications:

WB: 1:3000~1:10000, IHC: 1:200, IF/ICC: 1:200, ELISA(peptide) 1:20000-1:40000

Source:

Mouse

Reactivity:

Human,Mouse,Rat,Pig,Zebrafish,Bovine,Sheep,Rabbit,Goat,Guinea pig,Dog,Monkey,Hamster,Chicken,Plants,Rice,Fish

Clonality:

Monoclonal (F929)

Concentration:

1mg/ml

Molecular Weight:

Observed: 43KD. Predicted: 42kDa.

beta-Actin Mouse Monoclonal Antibody Western blot analysis of various lysates, using beta-Actin Mouse Monoclonal Antibody. Western blot analysis of various lysates, using beta-Actin Mouse Monoclonal Antibody.
beta-Actin Mouse Monoclonal Antibody IF analysis of Hela with T0022 diluted at 1:500. IF analysis of Hela with T0022 diluted at 1:500.

Purification:

Affinity-chromatography.

Specificity:

beta-Actin Mouse Monoclonal antibody detects endogenous levels of total beta-Actin protein.

Immunogen:

Full-length beta-Actin protein of human.

Uniprot:

Gene id:

Show more↓

Research Fields:

· Cellular Processes > Transport and catabolism > Phagosome.(View pathway)
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.(View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction.(View pathway)
· Cellular Processes > Cell growth and death > Apoptosis.(View pathway)
· Cellular Processes > Cellular community - eukaryotes > Adherens junction.(View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.(View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.(View pathway)
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.(View pathway)

Description:

beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.

Function:

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Subcellular Location:

Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Post-translational Modifications:

ISGylated.Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity).Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).

Subunit Structure:

Interacts with CPNE1 (via VWFA domain) and CPNE4 (via VWFA domain) (By similarity). Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCSAM. Interacts with TBC1D21 (By similarity). Interacts with DHX9 (via C-terminus); this interaction is direct and mediates the attachment to nuclear ribonucleoprotein complexes (PubMed:11687588).

Similarity:

Belongs to the actin family.

Protein BLAST With

Sequence:

        10         20         30         40         50
MDDDIAALVV DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGVMVGMGQK
60 70 80 90 100
DSYVGDEAQS KRGILTLKYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE
110 120 130 140 150
HPVLLTEAPL NPKANREKMT QIMFETFNTP AMYVAIQAVL SLYASGRTTG
160 170 180 190 200
IVMDSGDGVT HTVPIYEGYA LPHAILRLDL AGRDLTDYLM KILTERGYSF
210 220 230 240 250
TTTAEREIVR DIKEKLCYVA LDFEQEMATA ASSSSLEKSY ELPDGQVITI
260 270 280 290 300
GNERFRCPEA LFQPSFLGME SCGIHETTFN SIMKCDVDIR KDLYANTVLS
310 320 330 340 350
GGTTMYPGIA DRMQKEITAL APSTMKIKII APPERKYSVW IGGSILASLS
360 370
TFQQMWISKQ EYDESGPSIV HRKCF

Storage Condition and Buffer:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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Catalog Number :

T0022-BP

Price/Size :

$200/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

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