Price Size
$150 50ul
$250 100ul
$350 200ul
$1200 1ml

Same day delivery


For pricing and ordering contact:

local distributors
  • Product Name
    His-Tag Antibody
  • Catalog No.
    T0009
  • RRID
    AB_2839416
  • Source
    Mouse
  • Application
    WB,IF/ICC,IP,ELISA
  • Reactivity
    All
  • Concentration
    1mg/ml

Product Information

Alternative Names:Expand▼

his tag

Applications:

WB: 1:3000-1:10000, IF/ICC: 1:200, IP 1:100-1:500
*The optimal dilutions should be determined by the end user.

Reactivity:

All

Source:

Mouse

Clonality:

Monoclonal

Clone Number:

T113

Purification:

Affinity-chromatography.

Specificity:

His-Tag Mouse Monoclonal antibody detects endogenous levels of internal, C-terminal, or N-terminal 6His -tagged proteins.

RRID:

AB_2839416
Please cite this product as: Affinity Biosciences Cat# T0009, RRID:AB_2839416.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthetic peptide HHHHHH coupled to KLH.

Molecular Weight:

Observed Mol.Wt.: Not available.
Predicted Mol.Wt.: Not available.

Subcellular Location:

Endoplasmic reticulum membrane. Microsome membrane.

Description:

N/A

Reference Citations:

1). Liu X;Li Y;Wang M;Wang X;Zhang L;Peng T;Liang W;Wang Z;Lu H; et al. The 1316T> C missenses mutation in MTHFR contributes to MTHFR deficiency by targeting MTHFR to proteasome degradation. Aging (Albany NY) 2020 Dec 3;12. (PubMed: 33290257) [IF=4.831]

2). Ge S;Xu L;Li B;Zhong F;Liu X;Zhang X; et al. Canine Parvovirus is diagnosed and neutralized by chicken IgY-scFv generated against the virus capsid protein. Vet Res 2020 Sep 3;51(1):110. (PubMed: 32883344) [IF=3.357]

3). Duan Z;Han Y;Zhou L;Yuan C;Wang Y;Zhao C;Tang H;Chen J; et al. Chicken bromodomain-containing protein 2 interacts with the Newcastle disease virus matrix protein and promotes viral replication. Vet Res 2020 Sep 22;51(1):120. (PubMed: 32962745) [IF=3.357]

4). Wang JL;Chen WG;Zhang JJ;Xu CJ; et al. Nogo-A-Δ20/EphA4 interaction antagonizes apoptosis of neural stem cells by integrating p38 and JNK MAPK signaling. J Mol Histol 2021 Jun;52(3):521-537. (PubMed: 33555537)

5). et al. A sensitive and reversible staining of proteins on blot membranes.

6). et al. Generation and Evaluation of Chicken IgY-scFv for the Purposes of CPV Diagnosis and Therapy.

7). et al. Study on β-Chitosan against the binding of SARS-CoV-2S-RBD/ACE2.

No comment
Total 0 records, divided into1 pages. First Prev Next Last

Submit Review

Support JPG, GIF, PNG format only
captcha
Catalog Number :

T0009-BP
(Blocking peptide available as T0009-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

To Top