Price Size
$150 50ul
$250 100ul
$350 200ul
$1200 1ml

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  • Product Name
    HA-Tag Antibody
  • Catalog No.
    T0008
  • RRID
    AB_2839415
  • Source
    Mouse
  • Application
    WB,IF/ICC,IP,ELISA
  • Reactivity
    All
  • Concentration
    1mg/ml

Product Information

Alternative Names:Expand▼

ha tag

Applications:

WB: 1:3000-1:10000, IF/ICC: 1:200, IP 1:200
*The optimal dilutions should be determined by the end user.

Reactivity:

All

Source:

Mouse

Clonality:

Monoclonal

Clone Number:

T80

Purification:

Affinity-chromatography.

Specificity:

HA-Tag Mouse Monoclonal antibody detects endogenous levels of internal, C-terminal, or N-terminal HA-tagged recombinant proteins.

RRID:

AB_2839415
Please cite this product as: Affinity Biosciences Cat# T0008, RRID:AB_2839415.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,.

Immunogen Information in 3D

Immunogen:

A synthetic peptide YPYDVPDYA coupled to KLH.

Molecular Weight:

Observed Mol.Wt.: Not available.
Predicted Mol.Wt.: Not available.

Subcellular Location:

Endoplasmic reticulum membrane. Microsome membrane.

Description:

N/A

Reference Citations:

1). Liu T et al. The m6A reader YTHDF1 promotes ovarian cancer progression via augmenting EIF3C translation. Nucleic Acids Res 2020 Jan 30 (PubMed: 31996915) [IF=11.501]

2). Zhang H et al. Oryza sativa POSITIVE REGULATOR OF IRON DEFICIENCY RESPONSE 2 (OsPRI2) and OsPRI3 are involved in the maintenance of Fe homeostasis. Plant Cell Environ 2020 Jan;43(1):261-274 (PubMed: 31674679) [IF=6.362]

3). Yin X et al. The Nuclear-Localized RxLR Effector PvAvh74 From Plasmopara viticola Induces Cell Death and Immunity Responses in Nicotiana benthamiana. Front Microbiol 2019 Jul 10;10:1531 (PubMed: 31354650) [IF=4.235]

4). Yin X et al. The Nuclear-Localized RxLR Effector PvAvh74 From Plasmopara viticola Induces Cell Death and Immunity Responses in Nicotiana benthamiana. Front Microbiol 2019 Jul 10;10:1531 (PubMed: 31354650) [IF=4.235]

5). Duan Z;Han Y;Zhou L;Yuan C;Wang Y;Zhao C;Tang H;Chen J; et al. Chicken bromodomain-containing protein 2 interacts with the Newcastle disease virus matrix protein and promotes viral replication. Vet Res 2020 Sep 22;51(1):120. (PubMed: 32962745) [IF=3.357]

6). Sun L;Büeler H; et al. Proteasome inhibition promotes mono-ubiquitination and nuclear translocation of mature (52 kDa) PINK1. Biochem Biophys Res Commun 2019 Sep 17;517(2):376-382. (PubMed: 31362890)

7). et al. Assessment of SENP3-interacting proteins in hepatocytes treated with diethylnitrosamine by BioID assay.

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Catalog Number :

T0008-BP
(Blocking peptide available as T0008-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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