Product Info

WB: 1:3000-1:10000
*The optimal dilutions should be determined by the end user.
Monoclonal [T571]
GST-Tag Mouse Monoclonal antibody detects endogenous levels of C-terminal, internal, and N-terminal GST-tagged proteins.
Cite Format: Affinity Biosciences Cat# T0007, RRID:AB_2839414.
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.



Full-length GST protein.


1). Zhou H et al. miR-506 enhances the sensitivity of human colorectal cancer cells to oxaliplatin by suppressing MDR1/P-gp expression. Cell Prolif 2017 Jun;50(3) (PubMed: 28217977) [IF=5.753]

Application: WB    Species: human    Sample:

FIGURE 3  miR-506 down-regulated MDR1/P-gp expression in HCT116-OxR. A, The mRNA level of MDR1 was decreased after transfection with the miR-506 mimic of the relative chemoresistant genes as demonstrated by qRT-PCR. B, The protein level of MDR1 was decreased after transfection with the miR-506 mimic of the relative chemoresistant proteins as demonstrated by Western blot. C, Expression of P-gp detected by immunofluorescence staining.HCT116-OxR-miR-506 cells showed low levels of fluorescent staining of P-gp, whereas maximal staining of P-gp was observed in HCT116-OxR cells, readily distinguished from background. Zoom: 200×. *P<.05)

2). Shi Z et al. Hepatoprotective and Anti-Oxidative Effects of Total Flavonoids From Qu Zhi Qiao (Fruit of Citrus Paradisi cv.Changshanhuyou) on Nonalcoholic Steatohepatitis In Vivo and In Vitro Through Nrf2-ARE Signaling Pathway. Front Pharmacol 2020 Apr 22;11:483 (PubMed: 32390839) [IF=4.225]

Application: WB    Species: mouse    Sample: liver

FIGURE 5 | Expression of the targeted proteins in mice liver. Values are shown as mean ± SD (n = 8). Data (mean ± SD) with different case letters are statistically different with each other at LSD multiple comparisons, which with red letters differed significantly as compared to model group level. uppercase letter p < 0.01 vs.another group.

3). Duan Z et al. Chicken bromodomain-containing protein 2 interacts with the Newcastle disease virus matrix protein and promotes viral replication. Vet Res 2020 Sep 22;51(1):120. (PubMed: 32962745) [IF=3.357]

Application: WB    Species:    Sample:

Figure 1| Identifcation of the interaction between the NDV M protein and chBRD2 protein.C A Coomassie stained gel showing the bacterial expression purifed proteins. The His-tagged chBRD2 (His-chBRD2), His tag,GST-tagged M (GST-M), and GST tag were expressed in E. coli BL21 (DE3), and the soluble His-chBRD2 and His tag or GST-M and GST tag were purifed on Ni–NTA His*Bind Resin or Glutathione-Sepharose 4B beads, respectively. The bacterial expression purifed proteins were detected by SDS-PAGE along with Coomassie blue staining. Identifcation of the interaction between the M protein and chBRD2 protein by His pull-down assay (D) and GST pull-down assay (E).

4). Plant AFC2 kinase desensitizes thermomorphogenesis through modulation of alternative splicing.

5). Lin J et al. Plant AFC2 kinase desensitizes thermomorphogenesis through modulation of alternative splicing. iScience 2022 Mar 11;25(4):104051. (PubMed: 35345463)

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