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$150 50ul
$250 100ul
$350 200ul
$1200 1ml

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  • Product Name
    GST-Tag Antibody
  • Catalog No.
    T0007
  • RRID
    AB_2839414
  • Source
    Mouse
  • Application
    WB,ELISA
  • Reactivity
    All
  • Concentration
    1mg/ml

Product Information

Applications:

WB: 1:3000-1:10000
*The optimal dilutions should be determined by the end user.

Reactivity:

All

Source:

Mouse

Clonality:

Monoclonal

Clone Number:

T571

Purification:

Affinity-chromatography.

Specificity:

GST-Tag Mouse Monoclonal antibody detects endogenous levels of C-terminal, internal, and N-terminal GST-tagged proteins.

RRID:

AB_2839414
Please cite this product as: Affinity Biosciences Cat# T0007, RRID:AB_2839414.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

Full-length GST protein.

Molecular Weight:

Observed Mol.Wt.: Not available.
Predicted Mol.Wt.: Not available.

Subcellular Location:

Endoplasmic reticulum membrane. Microsome membrane.

Reference Citations:

1). Zhou H et al. miR-506 enhances the sensitivity of human colorectal cancer cells to oxaliplatin by suppressing MDR1/P-gp expression. Cell Prolif 2017 Jun;50(3) (PubMed: 28217977) [IF=5.753]

Application: WB    Species:human;    Sample:Not available

FIGURE 3  miR-506 down-regulated MDR1/P-gp expression in HCT116-OxR. A, The mRNA level of MDR1 was decreased after transfection with the miR-506 mimic of the relative chemoresistant genes as demonstrated by qRT-PCR. B, The protein level of MDR1 was decreased after transfection with the miR-506 mimic of the relative chemoresistant proteins as demonstrated by Western blot. C, Expression of P-gp detected by immunofluorescence staining.HCT116-OxR-miR-506 cells showed low levels of fluorescent staining of P-gp, whereas maximal staining of P-gp was observed in HCT116-OxR cells, readily distinguished from background. Zoom: 200×. *P<.05)


2). Shi Z et al. Hepatoprotective and Anti-Oxidative Effects of Total Flavonoids From Qu Zhi Qiao (Fruit of Citrus Paradisi cv.Changshanhuyou) on Nonalcoholic Steatohepatitis In Vivo and In Vitro Through Nrf2-ARE Signaling Pathway. Front Pharmacol 2020 Apr 22;11:483 (PubMed: 32390839) [IF=4.225]

3). Duan Z;Han Y;Zhou L;Yuan C;Wang Y;Zhao C;Tang H;Chen J; et al. Chicken bromodomain-containing protein 2 interacts with the Newcastle disease virus matrix protein and promotes viral replication. Vet Res 2020 Sep 22;51(1):120. (PubMed: 32962745) [IF=3.357]

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Catalog Number :

T0007-BP
(Blocking peptide available as T0007-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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