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  • Product Name
    NLRP3 Antibody
  • Catalog No.
    DF7438
  • RRID
    AB_2839376
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC,ELISA(peptide)
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Pig, Bovine, Horse, Sheep, Rabbit, Dog
  • UniProt
  • Mol.Wt
    80~120kD;
    118kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

AGTAVPRL; AII/AVP; Angiotensin/vasopressin receptor AII/AVP like; Angiotensin/vasopressin receptor AII/AVP-like; C1orf7; Caterpiller protein 1.1; CIAS 1; CIAS1; CLR1.1; Cold autoinflammatory syndrome 1; Cold autoinflammatory syndrome 1 protein; Cryopyrin; Familial cold autoinflammatory syndrome; FCAS; FCU; LRR and PYD domains-containing protein 3; Muckle-Wells syndrome; MWS; NACHT; NACHT LRR and PYD containing protein 3; NALP 3; NALP3; NALP3_HUMAN; NLR family pyrin domain containing 3; NLRP3; PYPAF 1; PYPAF1; PYRIN containing APAF1 like protein 1; PYRIN-containing APAF1-like protein 1;

Applications:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Pig, Bovine, Horse, Sheep, Rabbit, Dog

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

NLRP3 Antibody detects endogenous levels of total NLRP3.

RRID:

AB_2839376
Please cite this product as: Affinity Biosciences Cat# DF7438, RRID:AB_2839376.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthesized peptide derived from human NLRP3

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

NLRP3

Molecular Weight:

Observed Mol.Wt.: 80~120kD.
Predicted Mol.Wt.: 118kDa(Calculated)..

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Q96P20 NLRP3_HUMAN:
Predominantly expressed in macrophages. Also expressed in dendritic cells, B- and T-cells (at protein level) (PubMed:11786556) (PubMed:17164409). Expressed in LPS-treated granulocytes, but not in resting cells (at protein level) (PubMed:17164409). Expression in monocytes is very weak (at protein level) (PubMed:17164409). Expressed in stratified non-keratinizing squamous epithelium, including oral, esophageal and ectocervical mucosa and in the Hassall's corpuscles in the thymus. Also, detected in the stratified epithelium covering the bladder and ureter (transitional mucosa) (at protein level) (PubMed:17164409). Expressed in lung epithelial cells (at protein level) (PubMed:23229815). Expressed in chondrocytes (PubMed:12032915). Expressed at low levels in resting osteoblasts (PubMed:17907925).

Description:

This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid.

Sequence:
MKMASTRCKLARYLEDLEDVDLKKFKMHLEDYPPQKGCIPLPRGQTEKADHVDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPKWGSDNARVSNPTVICQEDSIEEEWMGLLEYLSRISICKMKKDYRKKYRKYVRSRFQCIEDRNARLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDEHSEPVHTVVFQGAAGIGKTILARKMMLDWASGTLYQDRFDYLFYIHCREVSLVTQRSLGDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDWQKAERGDILLSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEAQARAAFSLIQENEVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLSSLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKILFEESDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKLQIQPSQLELFYCLYEMQEEDFVQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESLSLGFLHNMPKEEEEEEKEGRHLDMVQCVLPSSSHAACSHGLVNSHLTSSFCRGLFSVLSTSQSLTELDLSDNSLGDPGMRVLCETLQHPGCNIRRLWLGRCGLSHECCFDISLVLSSNQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLKKLWLVSCCLTSACCQDLASVLSTSHSLTRLYVGENALGDSGVAILCEKAKNPQCNLQKLGLVNSGLTSVCCSALSSVLSTNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW

Research Background

Function:

As the sensor component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP3, PYCARD and CASP1 (and possibly CASP4 and CASP5). Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. Activation of NLRP3 inflammasome is also required for HMGB1 secretion. The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death. Under resting conditions, NLRP3 is autoinhibited. NLRP3 activation stimuli include extracellular ATP, reactive oxygen species, K(+) efflux, crystals of monosodium urate or cholesterol, amyloid-beta fibers, environmental or industrial particles and nanoparticles, cytosolic dsRNA, etc. However, it is unclear what constitutes the direct NLRP3 activator. Activation in presence of cytosolic dsRNA is mediated by DHX33. Independently of inflammasome activation, regulates the differentiation of T helper 2 (Th2) cells and has a role in Th2 cell-dependent asthma and tumor growth (By similarity). During Th2 differentiation, required for optimal IRF4 binding to IL4 promoter and for IRF4-dependent IL4 transcription. Binds to the consensus DNA sequence 5'-GRRGGNRGAG-3'. May also participate in the transcription of IL5, IL13, GATA3, CCR3, CCR4 and MAF (By similarity).

Post-translational Modifications:

The disulfide bond in the pyrin domain might play a role in reactive oxygen species-mediated activation.

Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Ubiquitination does not lead to degradation, but inhibits inflammasome activation (By similarity). Deubiquitination is catalyzed by BRCC3 and associated with NLRP3 activation and inflammasome assembly. This process can be induced by the activation of Toll-like receptors (by LPS), through a non-transcriptional pathway dependent on the mitochondrial production of reactive oxygen species, and by ATP.

Subcellular Location:

Cytoplasm>Cytosol. Inflammasome. Endoplasmic reticulum. Secreted. Nucleus.
Note: In macrophages, under resting conditions, mainly located in the cytosol, on the endoplasmic reticulum. After stimulation with inducers of the NLRP3 inflammasome, mitochondria redistribute in the vicinity of the endoplasmic reticulum in the perinuclear region, which results in colocalization of NLRP3 on the endoplasmic reticulum and PYCARD on mitochondria, allowing the activation of inflammasome assembly. After the induction of pyroptosis, inflammasome specks are released into the extracellular space where they can further promote IL1B processing and where they can be engulfed by macrophages. Phagocytosis induces lysosomal damage and inflammasome activation in the recipient cells (PubMed:24952504). In the Th2 subset of CD4(+) helper T-cells, mainly located in the nucleus. Nuclear localization depends upon KPNA2. In the Th1 subset of CD4(+) helper T-cells, mainly cytoplasmic (By similarity).

Golgi apparatus membrane.
Note: (Microbial infection) Upon HRSV infection, the protein is mainly located in lipid rafts in the Golgi membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

Predominantly expressed in macrophages. Also expressed in dendritic cells, B- and T-cells (at protein level). Expressed in LPS-treated granulocytes, but not in resting cells (at protein level). Expression in monocytes is very weak (at protein level). Expressed in stratified non-keratinizing squamous epithelium, including oral, esophageal and ectocervical mucosa and in the Hassall's corpuscles in the thymus. Also, detected in the stratified epithelium covering the bladder and ureter (transitional mucosa) (at protein level). Expressed in lung epithelial cells (at protein level). Expressed in chondrocytes. Expressed at low levels in resting osteoblasts.

Subunit Structure:

Sensor component of NLRP3 inflammasomes. Inflammasomes are supramolecular complexes that assemble in the cytosol in response to pathogens and other damage-associated signals and play critical roles in innate immunity and inflammation. The core of NLRP3 inflammasomes consists of a signal sensor component (NLRP3), an adapter (ASC/PYCARD), which recruits an effector proinflammatory caspase (CASP1 and, possibly, CASP4 and CASP5). Within the complex, NLRP3 and PYCARD interact via their respective DAPIN/pyrin domains. This interaction initiates speck formation (nucleation) which greatly enhances further addition of soluble PYCARD molecules to the speck in a prion-like polymerization process. NLRP3 localizes at the end of each PYCARD filament. Clustered PYCARD nucleates the formation of CASP1 filaments through the interaction of their respective CARD domains, acting as a platform for CASP1 polymerization. CASP1 filament formation increases local enzyme concentration, resulting in trans-autocleavage and activation. Active CASP1 then processes IL1B and IL18 precursors, leading to the release of mature cytokines in the extracellular milieu and inflammatory response. Reconstituted ternary inflammasomes show star-shaped structures, in which multiple filaments, containing CASP1, protrude radially from a single central hub, containing the sensor protein and PYCARD. In this complex, the sensor protein is sub-stoichiometric to PYCARD, and PYCARD is further substoichiometric to CASP1, suggesting amplifications of signal transduction from the sensor, via the adapter, to the effector. Interacts with MEFV; this interaction targets NLRP3 to degradation by autophagy, hence preventing excessive IL1B- and IL18-mediated inflammation. Interacts with GBP5 (via DAPIN domain); this interaction promotes inflammasome assembly in response to microbial and soluble, but not crystalline, agents. Interacts with EIF2AK2/PKR; this interaction requires EIF2AK2 activity, is accompanied by EIF2AK2 autophosphorylation and promotes inflammasome assembly in response to specific stimuli. Interacts with PML (isoform PML-1) (via the leucine-rich repeat (LRR) domain); PML-mediated increase in NLRP3 inflammasome activation does not depend upon this interaction. Directly interacts with IRF4 (via the LRR domain); this interaction is required for optimal IRF4 binding to IL4 promoter and efficient IL4 transactivation during differentiation of Th2 helper T-cells (By similarity). Interacts (via NACHT domain) with DHX33 (via DEAH box). Interacts with PYDC5.

Similarity:

The pyrin domain (also called DAPIN domain or PYD) is involved in PYCARD-binding.

The LRR domain mediates the interaction with IRF4 and PML.

Intramolecular interactions between NACHT and leucine-rich repeat (LRR) domains may be important for autoinhibition in the absence of activating signal.

Belongs to the NLRP family.

Research Fields

Research Fields:

· Cellular Processes > Cell growth and death > Necroptosis.(View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pertussis.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Organismal Systems > Immune system > NOD-like receptor signaling pathway.(View pathway)

Reference Citations:

1). Li X et al. Activation of NLRP3 in microglia exacerbates diesel exhaust particles-induced impairment in learning and memory in mice. Environ Int 2020 Mar;136:105487 (PubMed: 31999974) [IF=7.577]

2). Su P;Wang D;Cao Z;Chen J;Zhang J; et al. The role of NLRP3 in lead-induced neuroinflammation and possible underlying mechanism. Environ Pollut 2021 Oct 15;287:117520. (PubMed: 34182382) [IF=6.792]

3). Du L et al. Novel biphenyl diester derivative AB-38b inhibits NLRP3 inflammasome through Nrf2 activation in diabetic nephropathy. Cell Biol Toxicol 2019 Nov 25 (PubMed: 31768838) [IF=6.284]

4). Li XX et al. Protective effects of acarbose against vascular endothelial dysfunction through inhibiting Nox4/NLRP3 inflammasome pathway in diabetic rats. Free Radic Biol Med 2019 Sep 18 (PubMed: 31541678) [IF=6.170]

5). Zhu JJ;Yu BY;Huang XK;He MZ;Chen BW;Chen TT;Fang HY;Chen SQ;Fu XQ;Li PJ;Lin ZL;Zhu JH; et al. Neferine Protects against Hypoxic-Ischemic Brain Damage in Neonatal Rats by Suppressing NLRP3-Mediated Inflammasome Activation. Oxid Med Cell Longev 2021 May 8;2021:6654954. (PubMed: 34046147) [IF=5.076]

6). Park JH;Choi JY;Lee HK;Jo C;Koh YH; et al. Notch1-mediated inflammation is associated with endothelial dysfunction in human brain microvascular endothelial cells upon particulate matter exposure. Arch Toxicol 2020 Nov 7. (PubMed: 33159583) [IF=5.059]

7). Ding S et al. Resveratrol alleviates chronic "real-world" ambient particulate matter-induced lung inflammation and fibrosis by inhibiting NLRP3 inflammasome activation in mice. Ecotoxicol Environ Saf 2019 Oct 30;182:109425 (PubMed: 31295660) [IF=4.872]

8). Bai Y;Liu D;Zhang H;Wang Y;Wang D;Cai H;Wen H;Yuan G;An H;Wang Y;Shi T;Wang Z; et al. N-salicyloyl tryptamine derivatives as potential therapeutic agents for Alzheimer's disease with neuroprotective effects. Bioorg Chem 2021 Aug 14;115:105255. (PubMed: 34435574) [IF=4.831]

9). Ge L;Lin Z;Le G;Hou L;Mao X;Liu S;Liu D;Gan F;Huang K; et al. Nontoxic-dose deoxynivalenol aggravates lipopolysaccharides-induced inflammation and tight junction disorder in IPEC-J2 cells through activation of NF-κB and LC3B. Food Chem Toxicol 2020 Aug 30;145:111712. (PubMed: 32877744) [IF=4.679]

10). Li X;Li H;Cai D;Li P;Jin J;Jiang X;Li Z;Tian L;Chen G;Sun J;Bai W; et al. Chronic oral exposure to cadmium causes liver inflammation by NLRP3 inflammasome activation in pubertal mice. Food Chem Toxicol 2021 Feb;148:111944. (PubMed: 33359024) [IF=4.679]

11). Bai B;Yang Y;Ji S;Wang S;Peng X;Tian C;Sun RC;Yu T;Chu XM; et al. MicroRNA‐302c‐3p inhibits endothelial cell pyroptosis via directly targeting NOD‐, LRR‐and pyrin domain‐containing protein 3 in atherosclerosis. J Cell Mol Med 2021 Mar 30. (PubMed: 33783966) [IF=4.658]

12). Li L;Zeng X;Liu Z;Chen X;Li L;Luo R;Liu X;Zhang J;Liu J;Lu Y;Cheng J;Chen Y; et al. Mesenchymal stromal cells protect hepatocytes from lipotoxicity through alleviation of endoplasmic reticulum stress by restoring SERCA activity. J Cell Mol Med 2021 Feb 16. (PubMed: 33591626) [IF=4.658]

13). Bai B;Yang Y;Ji S;Wang S;Peng X;Tian C;Sun RC;Yu T;Chu XM; et al. MicroRNA‐302c‐3p inhibits endothelial cell pyroptosis via directly targeting NOD‐, LRR‐and pyrin domain‐containing protein 3 in atherosclerosis. J Cell Mol Med 2021 Mar 30. (PubMed: 33783966) [IF=4.486]

14). Li L;Zeng X;Liu Z;Chen X;Li L;Luo R;Liu X;Zhang J;Liu J;Lu Y;Cheng J;Chen Y; et al. Mesenchymal stromal cells protect hepatocytes from lipotoxicity through alleviation of endoplasmic reticulum stress by restoring SERCA activity. J Cell Mol Med 2021 Feb 16. (PubMed: 33591626) [IF=4.486]

15). He Q et al. Parkin-Dependent Mitophagy is Required for the Inhibition of ATF4 on NLRP3 Inflammasome Activation in Cerebral Ischemia-Reperfusion Injury in Rats. Cells 2019 Aug 14;8(8) (PubMed: 31416289) [IF=4.366]

16). Xu L;Lin G;Yu Q;Li Q;Mai L;Cheng J;Xie J;Liu Y;Su Z;Li Y; et al. Anti-Hyperuricemic and Nephroprotective Effects of Dihydroberberine in Potassium Oxonate-and Hypoxanthine-Induced Hyperuricemic Mice. Front Pharmacol 2021 Apr 20;12:645879. (PubMed: 33959014) [IF=4.225]

17). Hu F;Chen X;Gao J;Shen Y;Yang J; et al. CircDIP2C ameliorates oxidized low-density lipoprotein-induced cell dysfunction by binding to miR-556-5p to induce TET2 in human umbilical vein endothelial cells. Vascul Pharmacol 2021 Aug;139:106887. (PubMed: 34147657) [IF=4.152]

18). Lin JQ;Tian H;Zhao XG;Lin S;Li DY;Liu YY;Xu C;Mei XF; et al. Zinc provides neuroprotection by regulating NLRP3 inflammasome through autophagy and ubiquitination in a spinal contusion injury model. CNS Neurosci Ther 2020 Oct 9. (PubMed: 33034415) [IF=4.074]

19). Lu J;Wang X;Feng Z;Chen Y;Wen D;Liu Z; et al. The protective effect of isoflurane pretreatment on liver IRI by suppressing noncanonical pyroptosis of liver macrophages. Int Immunopharmacol 2021 Jul 29;99:107977. (PubMed: 34332342) [IF=3.943]

20). Gan W;Li X;Cui Y;Xiao T;Liu R;Wang M;Wei Y;Cui M;Ren S;Helian K;Ning W;Zhou H;Yang C; et al. Pinocembrin relieves lipopolysaccharide and bleomycin induced lung inflammation via inhibiting TLR4-NF-κB-NLRP3 inflammasome signaling pathway. Int Immunopharmacol 2020 Dec 3;90:107230. (PubMed: 33290968) [IF=3.943]

21). Gao Y;Li J;Wu Q;Wang S;Yang S;Li X;Chen N;Li L;Zhang L; et al. Tetrahydroxy stilbene glycoside ameliorates Alzheimer's disease in APP/PS1 mice via glutathione peroxidase related ferroptosis. Int Immunopharmacol 2021 Oct;99:108002. (PubMed: 34333354) [IF=3.943]

22). Wang X;Yin H;Fan L;Zhou Y;Tang X;Fei X;Tang H;Peng J;Zhang J;Xue Y;Luo J;Jin Q;Jin Q; et al. Shionone alleviates NLRP3 inflammasome mediated pyroptosis in interstitial cystitis injury. Int Immunopharmacol 2020 Nov 19;107132. (PubMed: 33223465) [IF=3.943]

23). Luo H;Xu N;Wu J;Gan Y;Chen L;Guan F;Li M;Li Y;Chen J;Su Z;Liu Y; et al. β-patchoulene protects against non-alcoholic steatohepatitis via interrupting the vicious circle among oxidative stress, histanoxia and lipid accumulation in rats. Int Immunopharmacol 2021 Sep;98:107915. (PubMed: 34198236) [IF=3.943]

24). Zhou J et al. Brusatol ameliorates 2, 4, 6-trinitrobenzenesulfonic acid-induced experimental colitis in rats: Involvement of NF-κB pathway and NLRP3 inflammasome. Int Immunopharmacol 2018 Nov;64:264-274 (PubMed: 30218953) [IF=3.943]

25). Jin J;Xie Y;Shi C;Ma J;Wang Y;Qiao L;Li K;Sun T; et al. Lipoxin A4 Inhibits NLRP3 Inflammasome Activation in Rats With Non-compressive Disc Herniation Through the JNK1/Beclin-1/PI3KC3 Pathway. Front Neurosci 2020 Sep 17;14:799. (PubMed: 33071721) [IF=3.707]

26). Chaturvedi S;Tiwari V;Gangadhar NM;Rashid M;Sultana N;Singh SK;Shukla S;Wahajuddin M; et al. Isoformononetin, a dietary isoflavone protects against streptozotocin induced rat model of neuroinflammation through inhibition of NLRP3/ASC/IL-1 axis activation. Life Sci 2021 Sep 28;286:119989. (PubMed: 34597609) [IF=3.647]

27). Lin X;Yang F;Huang J;Jiang S;Tang Y;Li J; et al. Ameliorate effect of pyrroloquinoline quinone against cyclophosphamide-induced nephrotoxicity by activating the Nrf2 pathway and inhibiting the NLRP3 pathway. Life Sci 2020 Jun 3;256:117901. (PubMed: 32504759) [IF=3.647]

28). Park JH et al. Involvement of ADAM10 in acrolein-induced astrocytic inflammation. Toxicol Lett 2019 Oct 19 (PubMed: 31639409) [IF=3.569]

29). Fang Q;Zheng B;Liu N;Liu J;Liu W;Huang X;Zeng X;Chen L;Li Z;Ouyang D; et al. Trimethylamine N-Oxide Exacerbates Renal Inflammation and Fibrosis in Rats With Diabetic Kidney Disease. Front Physiol 2021 Jun 16;12:682482. (PubMed: 34220546) [IF=3.367]

30). Lu K;Zhou J;Deng J;Li Y;Wu C;Bao J; et al. Periplaneta americana Oligosaccharides Exert Anti-Inflammatory Activity through Immunoregulation and Modulation of Gut Microbiota in Acute Colitis Mice Model. Molecules 2021 Mar 19;26(6):1718. (PubMed: 33808686) [IF=3.267]

31). Hu R et al. Salidroside ameliorates endothelial inflammation and oxidative stress by regulating the AMPK/NF-κB/NLRP3 signaling pathway in AGEs-induced HUVECs. Eur J Pharmacol 2019 Nov 17:172797 (PubMed: 31747547) [IF=3.263]

32). Yang K et al. Metabolomics Analysis Reveals Therapeutic Effects of α-Mangostin on Collagen-Induced Arthritis in Rats by Down-regulating Nicotinamide Phosphoribosyltransferase. Inflammation 2019 Apr;42(2):741-753 (PubMed: 30484004) [IF=3.212]

33). Zeng X et al. Oleic acid ameliorates palmitic acid induced hepatocellular lipotoxicity by inhibition of ER stress and pyroptosis. Nutr Metab (Lond) 2020 Jan 30;17:11 (PubMed: 32021639) [IF=3.211]

34). Fu J;Zhao B;Ni C;Ni H;Xu L;He Q;Xu M;Xu C;Luo G;Zhu J;Tao J;Yao M; et al. Rosiglitazone Alleviates Mechanical Allodynia of Rats with Bone Cancer Pain through the Activation of PPAR-γ to Inhibit the NF-κB/NLRP3 Inflammatory Axis in Spinal Cord Neurons. PPAR Res 2021 Aug 25;2021:6086265. (PubMed: 34484316)

35). Tian Q;Xu M;He B; et al. Histidine ameliorates elastase-and lipopolysaccharide-induced lung inflammation by inhibiting the activation of the NLRP3 inflammasome. Acta Biochim Biophys Sin (Shanghai) 2021 Jun 14;gmab072. (PubMed: 34125142)

36). Zeng H;Han X;Zhu Z;Yu S;Mei S;Cheng X;Zhang W;Zhang G;Fang D; et al. Increased uterine NLRP3 inflammasome and leucocyte infiltration in a rat model of preeclampsia. Am J Reprod Immunol 2021 Aug 10. (PubMed: 34375018)

37). Chen M et al. The Therapeutic Effects and Possible Mechanism of Pranoprofen in Mouse Model of Corneal Alkali Burns. J Ophthalmol 2020 Apr 6;2020:7485912 (PubMed: 32322412)

38). Chen L;Wang H;Ge S;Tai S; et al. IL-6/STAT3 pathway is involved in the regulation of autophagy in chronic non-bacterial prostatitis cells, and may be affected by the NLRP3 inflammasome. Ultrastruct Pathol 2021 Aug 23;1-10. (PubMed: 34423720)

39). Chen J;Zhang LG;Du HX;Zhan CS;Liu Y;Zhang M;Chen XG;Wen LP;Zhang L;Liang CZ; et al. Melatonin attenuates prostatic inflammation and pelvic pain via Sirt1-dependent inhibition of the NLRP3 inflammasome in an EAP mouse model. Prostate 2021 Nov;81(15):1179-1190. (PubMed: 34418127)

40). et al. Ketogenic Diet Ameliorates Lipid Dysregulation in Type 2 Diabetic Mice by Downregulating Hepatic Pescadillo 1.

41). et al. Expression and clinical significance of NLRP1 and NLRP3 in colonic tissues of patients with ulcerative colitis.

42). et al. Sesamol supplementation alleviates nonalcoholic steatohepatitis and atherosclerosis in high-fat, high carbohydrate and high-cholesterol diet-fed rats.

43). Jiang J;Zhang G;Yu M;Gu J;Zheng Y;Sun J;Ding S; et al. Quercetin improves the adipose inflammatory response and insulin signaling to reduce “real-world” particulate matter-induced insulin resistance. Environ Sci Pollut Res Int 2021 Aug 7. (PubMed: 34365603)

44). et al. Sonneratia apetala seed oil attenuates potassium oxonate/hypoxanthine-induced hyperuricemia and renal injury in mice.

45). et al. Callicarpa Nudiora Extract Exerts AntiInammatory Effect on H. Pylori-Associated Gastritis Through Repression of ROS/NLRP3/Caspase-1/IL-1β Signaling Ax.

46). et al. Resveratrol improves cardiac function and left ventricular fibrosis after myocardial infarction in rats by inhibiting NLRP3 inflammasome activity and the TGF-β1/SMAD2 signaling pathway.

47). et al. Gut microbiota-mediated transformation of coptisine into a novel metabolite 8-oxocoptisine: Insight into its superior anti-colitis effect.

48). et al. Inhibition of Caspase-1 Ameliorates Ischemia-Associated Blood-Brain Barrier Dysfunction and Integrity by Suppressing Pyroptosis Activation.

49). et al. Panax quinquefolius L. Saponins Protect Myocardial Ischemia Reperfusion No-Reflow Through Inhibiting the Activation of NLRP3 Inflammasome via TLR4/MyD88/NF-κB Signaling Pathway.

50). et al. Effect of sericin on the p38MAPK signaling pathway and NLRP3 inflammasome in the kidney of type 2 diabetic rats.

51). Wang H;Yang F;Xin R;Cui D;He J;Zhang S;Sun Y; et al. The gut microbiota attenuate neuroinflammation in manganese exposure by inhibiting cerebral NLRP3 inflammasome. Biomed Pharmacother 2020 Sep;129:110449. (PubMed: 32768944)

52). Yang SK;Han YC;He JR;Yang M;Zhang W;Zhan M;Li AM;Li L;Na-Song None;Liu YT;Wu XQ;Zhang Q;Wang JW;Zhang H; et al. Mitochondria targeted peptide SS-31 prevent on cisplatin-induced acute kidney injury via regulating mitochondrial ROS-NLRP3 pathway. Biomed Pharmacother 2020 Jul 24;130:110521. (PubMed: 32717631)

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Catalog Number :

DF7438-BP
(Blocking peptide available as DF7438-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
90%
Horse
90%
Bovine
90%
Sheep
90%
Rabbit
90%
Dog
89%
Xenopus
0%
Zebrafish
0%
Chicken
0%
High similarity Medium similarity Low similarity No similarity
Q96P20 as Substrate
Site PTM Type Enzyme
Ubiquitination
S5 Phosphorylation
Y13 Phosphorylation
S161 Phosphorylation
S163 Phosphorylation
S198 Phosphorylation
T233 Phosphorylation
S295 Phosphorylation
S334 Phosphorylation
S387 Phosphorylation
S436 Phosphorylation
K496 Ubiquitination
S728 Phosphorylation
Y861 Phosphorylation
S975 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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