GCLM Antibody | Affinity Biosciences

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Figure 3: Effects of oregano essential oil (OEO) on g-glutamylcysteine ligase (GCLM, GCLC), and Cu/Zn-superoxide dismutase (SOD1) expression in IPEC-J2 cells.

Figure 9.

FIGURE 5 | Expression of the targeted proteins in mice liver.

Fig.

Figure 6.

Product:	GCLM Antibody
Catalog:	DF7268
Description:	Rabbit polyclonal antibody to GCLM
Application:	WB IHC
Reactivity:	Human, Mouse, Rat, Pig
Prediction:	Pig, Bovine, Rabbit
Mol.Wt.:	30kDa; 31kD(Calculated).
Uniprot:	P48507
RRID:	AB_2839207

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Pig

Prediction:

Bovine(100%), Rabbit(100%)

Clonality:

Polyclonal

Specificity:

GCLM Antibody detects endogenous levels of total GCLM.

RRID:

AB_2839207
Cite Format: Affinity Biosciences Cat# DF7268, RRID:AB_2839207.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Gamma ECS regulatory subunit; Gamma-ECS regulatory subunit; Gamma-glutamylcysteine synthetase regulatory subunit; GCLM; GCS light chain; GLCLR; Glutamate cysteine ligase regulatory subunit; Glutamate--cysteine ligase modifier subunit; Glutamate--cysteine ligase regulatory subunit; GSC light chain; GSH0_HUMAN;

Immunogens

Immunogen:

Uniprot:

P48507(GSH0_HUMAN) >>Visit HPA database.

Gene(ID):

GCLM(2730)

Expression:

P48507 GSH0_HUMAN:

In all tissues examined. Highest levels in skeletal muscle.

Description:

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.

Sequence:

P48507 GSH0_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Rabbit

100

Xenopus

Horse

Sheep

Dog

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P48507 As Substrate

P48507

Site	PTM Type	Source
K9	Sumoylation	Uniprot
K9	Ubiquitination	Uniprot
T17	Phosphorylation	Uniprot
K34	Ubiquitination	Uniprot
C35	S-Nitrosylation	Uniprot
K49	Ubiquitination	Uniprot
C72	S-Nitrosylation	Uniprot
K80	Ubiquitination	Uniprot
K90	Ubiquitination	Uniprot
K158	Sumoylation	Uniprot
K158	Ubiquitination	Uniprot
T164	Phosphorylation	Uniprot
S165	Phosphorylation	Uniprot
K169	Ubiquitination	Uniprot
K205	Sumoylation	Uniprot
K263	Acetylation	Uniprot
K263	Ubiquitination	Uniprot
Y265	Phosphorylation	Uniprot
K270	Ubiquitination	Uniprot

Research Backgrounds

P48507_GSH0_HUMAN

Tissue Specificity:

In all tissues examined. Highest levels in skeletal muscle.

Subunit Structure:

Heterodimer of a catalytic heavy chain and a regulatory light chain.

Family&Domains:

Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Ferroptosis. (View pathway)

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Gly-Pro-Ala peptide and FGSHF3 exert protective effects in DON-induced toxicity and intestinal damage via decreasing oxidative stress. FOOD RESEARCH INTERNATIONAL (PubMed: 33509464) [IF=8.1]

2). Total flavonoids of Inula japonica alleviated the inflammatory response and oxidative stress in LPS-induced acute lung injury via inhibiting the sEH activity: Insights from lipid metabolomics. PHYTOMEDICINE (PubMed: 36150346) [IF=7.9]

3). Itaconate inhibits ferroptosis of macrophage via Nrf2 pathways against sepsis-induced acute lung injury. Cell Death Discovery (PubMed: 35110526) [IF=7.0]

Application: WB Species: Mice Sample: lung tissues

Fig. 3 4-OI inhibits ferroptosis through increasing and activating Nrf2 in vivo during ALI. A Western blots for Nrf2, GCLM, HO-1 and SLC7A11 in murine lung tissue. B–E Relative mRNA levels of Nrf2, GCLM, HO-1 and SLC7A11 in murine lung tissue (n = 6). F, G Relative levels of GSH and GSH/GSSG ratio in murine lung tissue (n = 6). H, I Representative images of fluorescence probe for ROS and its statistical results (n = 6) in lung tissue. (Data are presented as Mean ± SD *p < 0.05, **p < 0.01, ***p < 0.001).

4). Ameliorate effect of pyrroloquinoline quinone against cyclophosphamide-induced nephrotoxicity by activating the Nrf2 pathway and inhibiting the NLRP3 pathway. LIFE SCIENCES (PubMed: 32504759) [IF=6.1]

Application: WB Species: Mice Sample: kidney tissues

Fig. 6. Effect of PQQ on protein expression in the Nrf2/HO-1 pathway. The expression levels of Nrf2 (A), HO-1 (B), GCLM (C), and NQO1 (D). Data are shown as mean ± SD, n = 8. #p < 0.05 and ##p < 0.01 versus the Control group. ⁎ p < 0.05 and ⁎⁎p < 0.01 versus the Model group.

5). Therapeutic role of D-pinitol on experimental colitis via activating Nrf2/ARE and PPAR-γ/NF-κB signaling pathways. Food & Function (PubMed: 33625409) [IF=6.1]

6). Pyrroloquinoline quinone ameliorates liver injury in mice induced by cyclophosphamide. Environmental Science and Pollution Research (PubMed: 34997497) [IF=5.8]

7). Hepatoprotective and Anti-Oxidative Effects of Total Flavonoids From Qu Zhi Qiao (Fruit of Citrus Paradisi cv.Changshanhuyou) on Nonalcoholic Steatohepatitis In Vivo and In Vitro Through Nrf2-ARE Signaling Pathway. Frontiers in Pharmacology (PubMed: 32390839) [IF=5.6]

Application: WB Species: mouse Sample: liver

FIGURE 5 | Expression of the targeted proteins in mice liver. Values are shown as mean ± SD (n = 8). Data (mean ± SD) with different case letters are statistically different with each other at LSD multiple comparisons, which with red letters differed significantly as compared to model group level. uppercase letter p < 0.01 vs.another group.

8). Novel amphibian-derived antioxidant peptide protects skin against ultraviolet irradiation damage. Journal of photochemistry and photobiology-B, Biology (PubMed: 34628205) [IF=5.4]

9). Sodium Danshensu Cream Promotes the Healing of Pressure Ulcers in Mice through the Nrf2/HO-1 and NF-κB Pathways. Pharmaceuticals (PubMed: 36558999) [IF=4.6]

Application: WB Species: Mouse Sample:

Figure 6. Effect of SDSS on the protein levels of the Nrf2/HO-1 pathway (n = 5). (A) Western blot; the protein expression levels of Keap1 (B), Nrf2 (C), HO-1 (D), NQO1 (E), and GCLM (F) levels. * p < 0.05, ** p < 0.01 when compared to the control group. # p < 0.05, ## p < 0.01 when compared to the positive group.

10). Hepatoprotective effect of pyrroloquinoline quinone against alcoholic liver injury through activating Nrf2-mediated antioxidant and inhibiting TLR4-mediated inflammation responses. PROCESS BIOCHEMISTRY [IF=4.4]

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GCLM Antibody - #DF7268