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$350 200ul

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  • Product Name
    Annexin A6 Antibody
  • Catalog No.
    AF0116
  • RRID
    AB_2833266
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC,ELISA
  • Reactivity
    Human, Mouse
  • Prediction
    Pig(91%), Bovine(91%), Sheep(82%), Dog(91%)
  • UniProt
  • Mol.Wt
    75kD;
    76kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

67 kDa calelectrin; Annexin A6; Annexin VI; Annexin VI p68; Annexin-6; AnnexinA6; AnnexinVI; ANX 6; ANX A6; ANX6; ANXA 6; ANXA6; ANXA6_HUMAN; Calcium binding protein p68; Calelectrin; Calphobindin II; Calphobindin-II; CalphobindinII; CBP 68; CBP68; Chromobindin 20; Chromobindin-20; Chromobindin20; CPB II; CPB-II; CPBII; Lipocortin VI; LipocortinVI; p68; p70; Protein III; ProteinIII;

Applications:

WB: 1:500-1:3000, IHC: 1:50-1:200, IF/ICC: 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse

Predicted Reactivity:

Pig(91%), Bovine(91%), Sheep(82%), Dog(91%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

Annexin A6 Antibody detects endogenous levels of total Annexin A6.

RRID:

AB_2833266
Please cite this product as: Affinity Biosciences Cat# AF0116, RRID:AB_2833266.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human Annexin A6, corresponding to a region within N-terminal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

ANXA6

Molecular Weight:

Observed Mol.Wt.: 75kD.
Predicted Mol.Wt.: 76kDa(Calculated)..

Subcellular Location:

Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Description:

ANXA6 a calcium/phospholipid-binding protein that may associate with CD21. May regulate the release of Ca(2+) from intracellular stores. Phosphorylated in response to growth factor stimulation. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Sequence:
MAKPAQGAKYRGSIHDFPGFDPNQDAEALYTAMKGFGSDKEAILDIITSRSNRQRQEVCQSYKSLYGKDLIADLKYELTGKFERLIVGLMRPPAYCDAKEIKDAISGIGTDEKCLIEILASRTNEQMHQLVAAYKDAYERDLEADIIGDTSGHFQKMLVVLLQGTREEDDVVSEDLVQQDVQDLYEAGELKWGTDEAQFIYILGNRSKQHLRLVFDEYLKTTGKPIEASIRGELSGDFEKLMLAVVKCIRSTPEYFAERLFKAMKGLGTRDNTLIRIMVSRSELDMLDIREIFRTKYEKSLYSMIKNDTSGEYKKTLLKLSGGDDDAAGQFFPEAAQVAYQMWELSAVARVELKGTVRPANDFNPDADAKALRKAMKGLGTDEDTIIDIITHRSNVQRQQIRQTFKSHFGRDLMTDLKSEISGDLARLILGLMMPPAHYDAKQLKKAMEGAGTDEKALIEILATRTNAEIRAINEAYKEDYHKSLEDALSSDTSGHFRRILISLATGHREEGGENLDQAREDAQVAAEILEIADTPSGDKTSLETRFMTILCTRSYPHLRRVFQEFIKMTNYDVEHTIKKEMSGDVRDAFVAIVQSVKNKPLFFADKLYKSMKGAGTDEKTLTRIMVSRSEIDLLNIRREFIEKYDKSLHQAIEGDTSGDFLKALLALCGGED

Research Background

Function:

May associate with CD21. May regulate the release of Ca(2+) from intracellular stores.

Post-translational Modifications:

Phosphorylated in response to growth factor stimulation.

Subcellular Location:

Cytoplasm. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Similarity:

A pair of annexin repeats may form one binding site for calcium and phospholipid.

Belongs to the annexin family.

Review: good product

Anonymous | 2015-02-10


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Catalog Number :

AF0116-BP
(Blocking peptide available as AF0116-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
91%
Bovine
91%
Dog
91%
Sheep
82%
Xenopus
73%
Chicken
64%
Horse
57%
Rabbit
0%
Zebrafish
0%
High similarity Medium similarity Low similarity No similarity
P08133 as Substrate
Site PTM Type Enzyme
A2 Acetylation
K3 Acetylation
K9 Acetylation
K9 Ubiquitination
Y10 Phosphorylation
R11 Methylation
S13 Phosphorylation
Y30 Phosphorylation
K34 Ubiquitination
S38 Phosphorylation
K40 Acetylation
K40 Ubiquitination
S49 Phosphorylation
K63 Acetylation
K63 Ubiquitination
K68 Acetylation
K68 Ubiquitination
K75 Acetylation
K75 Ubiquitination
Y76 Phosphorylation
K81 Acetylation
K81 Ubiquitination
Y95 Phosphorylation
K99 Acetylation
K99 Ubiquitination
K102 Acetylation
K102 Ubiquitination
S106 Phosphorylation
K113 Ubiquitination
C114 S-Nitrosylation
S121 Phosphorylation
K135 Ubiquitination
S151 Phosphorylation
Y185 Phosphorylation
K220 Ubiquitination
K224 Ubiquitination
S229 Phosphorylation
S235 Phosphorylation
K240 Ubiquitination
K247 Ubiquitination
T252 Phosphorylation
R259 Methylation
K265 Acetylation
T269 Phosphorylation
T273 Phosphorylation
S282 Phosphorylation
Y297 Phosphorylation
K299 Acetylation
K299 Ubiquitination
S300 Phosphorylation
Y302 Phosphorylation
S303 Phosphorylation
K306 Acetylation
K306 Ubiquitination
S310 Phosphorylation
Y313 Phosphorylation
K314 Acetylation
Y340 Phosphorylation
T356 Phosphorylation
K370 Acetylation
K370 Ubiquitination
K377 Ubiquitination
T381 Phosphorylation
T391 Phosphorylation
K406 Ubiquitination
K418 Acetylation
K418 Ubiquitination
S419 Phosphorylation
S422 Phosphorylation
K442 Acetylation
K446 Ubiquitination
K456 Ubiquitination
T464 Phosphorylation
K478 Acetylation
K478 Ubiquitination
K483 Acetylation
K483 Ubiquitination
S490 Phosphorylation
S494 Phosphorylation
S503 Phosphorylation
T535 Phosphorylation
S537 Phosphorylation
K540 Ubiquitination
K568 Ubiquitination
Y572 Phosphorylation
K579 Ubiquitination
K580 Acetylation
S583 Phosphorylation
S596 Phosphorylation
K600 Ubiquitination
K607 Acetylation
K607 Ubiquitination
Y609 Phosphorylation
K613 Acetylation
K620 Acetylation
K620 Ubiquitination
S630 Phosphorylation
K644 Ubiquitination
K647 Acetylation
K647 Ubiquitination
T657 Phosphorylation
S658 Phosphorylation
K663 Ubiquitination
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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