CX3CR1 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from mouse fetal muscletissue lysates, using CX3CR1 antibody.

Product:	CX3CR1 Antibody
Catalog:	DF7096
Description:	Rabbit polyclonal antibody to CX3CR1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	44kDa; 40kD(Calculated).
Uniprot:	P49238
RRID:	AB_2839051

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

CX3CR1 Antibody detects endogenous levels of total CX3CR1.

RRID:

AB_2839051
Cite Format: Affinity Biosciences Cat# DF7096, RRID:AB_2839051.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Beta chemokine receptor-like 1; C X3 C CKR 1; C-X3-C CKR-1; CCRL1; Chemokine C X3 C motif receptor 1; CMK BRL 1; CMK-BRL-1; CMK-BRL1; CMKBLR1; CMKDR1; CX3C chemokine receptor 1; CX3C CKR1; CX3C1_HUMAN; CX3CR1; Fractalkine receptor; G-protein coupled receptor 13; GPR13; GPRV28; V28;

Immunogens

Immunogen:

Uniprot:

P49238(CX3C1_HUMAN) >>Visit HPA database.

Gene(ID):

CX3CR1(1524)

Expression:

P49238 CX3C1_HUMAN:

Expressed in lymphoid and neural tissues.

Description:

Fractalkine is a transmembrane protein and chemokine involved in the adhesion and migration of leukocytes. The protein encoded by this gene is a receptor for fractalkine. The encoded protein also is a coreceptor for HIV-1, and some variations in this gene lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Four transcript variants encoding two different isoforms have been found for this gene.

Sequence:

P49238 CX3C1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDQFPESVTENFEYDDLAEACYIGDIVVFGTVFLSIFYSVIFAIGLVGNLLVVFALTNSKKPKSVTDIYLLNLALSDLLFVATLPFWTHYLINEKGLHNAMCKFTTAFFFIGFFGSIFFITVISIDRYLAIVLAANSMNNRTVQHGVTISLGVWAAAILVAAPQFMFTKQKENECLGDYPEVLQEIWPVLRNVETNFLGFLLPLLIMSYCYFRIIQTLFSCKNHKKAKAIKLILLVVIVFFLFWTPYNVMIFLETLKLYDFFPSCDMRKDLRLALSVTETVAFSHCCLNPLIYAFAGEKFRRYLYHLYGKCLAVLCGRSVHVDFSSSESQRSRHGSVLSSNFTYHTSDGDALLLL

PTMs - P49238 As Substrate

P49238

Site	PTM Type	Source
Y128	Phosphorylation	Uniprot
S137	Phosphorylation	Uniprot
Y303	Phosphorylation	Uniprot
S329	Phosphorylation	Uniprot

Research Backgrounds

P49238_CX3C1_HUMAN

Function:

Receptor for the CX3C chemokine fractalkine (CX3CL1); binds to CX3CL1 and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1.

PTMs:

This protein is not N-glycosylated which is unusual for G-protein-coupled receptors.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Expressed in lymphoid and neural tissues.

Subunit Structure:

Found in a ternary complex with CX3CL1 and ITGAV:ITGB3 or ITGA4:ITGB1.

(Microbial infection) Interacts with human respiratory syncytial virus (HRSV) protein G; this interaction modulates host immune response.

(Microbial infection) Interacts with HIV-1 envelope polyprotein gp160.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)

Restrictive clause

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For Research Use Only.
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CX3CR1 Antibody - #DF7096