14-3-3 theta Antibody - #DF7016

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Product: 14-3-3 theta Antibody
Catalog: DF7016
Description: Rabbit polyclonal antibody to 14-3-3 theta
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.: 28kDa; 28kD(Calculated).
Uniprot: P27348
RRID: AB_2838972

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:50-1:100
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
14-3-3 theta Antibody detects endogenous levels of total 14-3-3 theta.
RRID:
AB_2838972
Cite Format: Affinity Biosciences Cat# DF7016, RRID:AB_2838972.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

14 3 3 protein T cell; 14 3 3 protein tau; 14 3 3 protein theta; 14 3 3 tau; 14 3 3 theta; 14-3-3 protein T-cell; 14-3-3 protein tau; 14-3-3 protein theta; 1433T_HUMAN; 1C5; HS1; KCIP1; Protein HS1; Protein kinase C inhibitor protein 1; Protein tau; tyr3/trp5 monooxygenase activation protein, theta; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, theta polypeptide; Ywhaq;

Immunogens

Immunogen:
Uniprot:

P27348(1433T_HUMAN) >>Visit HPA database.

Gene(ID):
YWHAQ(10971)
Expression:
P27348 1433T_HUMAN:

Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.

Description:
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.
Sequence:
MEKTELIQKAKLAEQAERYDDMATCMKAVTEQGAELSNEERNLLSVAYKNVVGGRRSAWRVISSIEQKTDTSDKKLQLIKDYREKVESELRSICTTVLELLDKYLIANATNPESKVFYLKMKGDYFRYLAEVACGDDRKQTIDNSQGAYQEAFDISKKEMQPTHPIRLGLALNFSVFYYEILNNPELACTLAKTAFDEAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDSAGEECDAAEGAEN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Chicken
100
Rabbit
100
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P27348 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K3 Acetylation
K3 Ubiquitination
K9 Acetylation
K9 Ubiquitination
K11 Acetylation
K11 Ubiquitination
Y19 Phosphorylation
T24 Phosphorylation
C25 S-Nitrosylation
K27 Acetylation
K27 Ubiquitination
S37 Phosphorylation
S45 Phosphorylation
Y48 Phosphorylation
K49 Acetylation
K49 Ubiquitination
S57 Phosphorylation
S64 Phosphorylation
K68 Acetylation
K68 Ubiquitination
K74 Ubiquitination
K75 Ubiquitination
K80 Acetylation
K80 Ubiquitination
Y82 Phosphorylation
K85 Acetylation
K85 Ubiquitination
S88 Phosphorylation
S92 Phosphorylation
C94 S-Nitrosylation
T95 Phosphorylation
K103 Ubiquitination
T110 Phosphorylation
S114 Phosphorylation
K115 Acetylation
K115 Ubiquitination
K120 Acetylation
K120 Ubiquitination
K122 Ubiquitination
Y125 Phosphorylation
Y128 Phosphorylation
C134 S-Nitrosylation
K139 Acetylation
K139 Ubiquitination
T141 Phosphorylation
S145 Phosphorylation
Y149 Phosphorylation
S156 Phosphorylation
K157 Acetylation
K157 Ubiquitination
K158 Acetylation
K158 Ubiquitination
K193 Ubiquitination
T205 Phosphorylation
S210 Phosphorylation
Y211 Phosphorylation
K212 Methylation
S214 Phosphorylation
T215 Phosphorylation
T229 Phosphorylation
S230 Phosphorylation
S232 Phosphorylation P11274 (BCR) , P48729 (CSNK1A1)

Research Backgrounds

Function:

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.

PTMs:

Ser-232 is probably phosphorylated by CK1.

Subcellular Location:

Cytoplasm.
Note: In neurons, axonally transported to the nerve terminals.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.

Subunit Structure:

Homodimer. Interacts with CDK16 (By similarity). Interacts with RGS7 (phosphorylated form). Interacts with SSH1. Interacts with CDKN1B ('Thr-198' phosphorylated form); the interaction translocates CDKN1B to the cytoplasm. Interacts with GAB2. Interacts with the 'Ser-241' phosphorylated form of PDPK1. Interacts with the 'Thr-369' phosphorylated form of DAPK2. Interacts with PI4KB, TBC1D22A and TBC1D22B. Interacts with SLITRK1. Interacts with RIPOR2 isoform 2. Interacts with INAVA; the interaction increases upon PRR (pattern recognition receptor) stimulation and is required for cellular signaling pathway activation and cytokine secretion. Interacts with MARK2, MARK3 and MARK4.

Family&Domains:

Belongs to the 14-3-3 family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

References

1). METTL3 preferentially enhances non-m6A translation of epigenetic factors and promotes tumourigenesis. Nature Cell Biology, 2022 (PubMed: 35927451) [IF=21.3]

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