NUMBL Antibody - #DF6863

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Product: NUMBL Antibody
Catalog: DF6863
Description: Rabbit polyclonal antibody to NUMBL
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 65kDa; 65kD(Calculated).
Uniprot: Q9Y6R0
RRID: AB_2838822

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(83%), Bovine(100%), Horse(89%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
NUMBL Antibody detects endogenous levels of total NUMBL.
RRID:
AB_2838822
Cite Format: Affinity Biosciences Cat# DF6863, RRID:AB_2838822.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CAG 3A; CAG3A; CTG 3a; CTG3a; NBL; NUMB Drosophilia Homolog Like; Numb homolog (Drosophila) like; Numb homolog like; Numb like protein; NUMB R; Numb-like protein; Numb-R; Numb-related gene; Numb-related protein; NUMBL; NUMBL_HUMAN; NUMBLIKE; NUMBR; TNRC 23; TNRC23; Trinucleotide repeat-containing gene 23;

Immunogens

Immunogen:
Uniprot:

Q9Y6R0(NUMBL_HUMAN) >>Visit HPA database.

Gene(ID):
NUMBL(9253)
Description:
Numb-like (Numbl or Nbl) binds Notch and is an intracellular modulator of Notch signaling pathway. Numbl contains a phosphotyrosine binding (PTB) domain that could bind the Y682 motif present in the cytoplasmic tail of APP. Deduced molecular weight of Numbl is 72 kDa.
Sequence:
MSRSAAASGGPRRPERHLPPAPCGAPGPPETCRTEPDGAGTMNKLRQSLRRRKPAYVPEASRPHQWQADEDAVRKGTCSFPVRYLGHVEVEESRGMHVCEDAVKKLKAMGRKSVKSVLWVSADGLRVVDDKTKDLLVDQTIEKVSFCAPDRNLDKAFSYICRDGTTRRWICHCFLALKDSGERLSHAVGCAFAACLERKQRREKECGVTAAFDASRTSFAREGSFRLSGGGRPAEREAPDKKKAEAAAAPTVAPGPAQPGHVSPTPATTSPGEKGEAGTPVAAGTTAAAIPRRHAPLEQLVRQGSFRGFPALSQKNSPFKRQLSLRLNELPSTLQRRTDFQVKGTVPEMEPPGAGDSDSINALCTQISSSFASAGAPAPGPPPATTGTSAWGEPSVPPAAAFQPGHKRTPSEAERWLEEVSQVAKAQQQQQQQQQQQQQQQQQQQQAASVAPVPTMPPALQPFPAPVGPFDAAPAQVAVFLPPPHMQPPFVPAYPGLGYPPMPRVPVVGITPSQMVANAFCSAAQLQPQPATLLGKAGAFPPPAIPSAPGSQARPRPNGAPWPPEPAPAPAPELDPFEAQWAALEGKATVEKPSNPFSGDLQKTFEIEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Horse
89
Pig
83
Rabbit
64
Zebrafish
57
Chicken
55
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6R0 As Substrate

Site PTM Type Enzyme
S48 Phosphorylation
T209 Phosphorylation
S224 Phosphorylation
S228 Phosphorylation
T251 Phosphorylation
S263 Phosphorylation
T265 Phosphorylation
T268 Phosphorylation
T269 Phosphorylation
S270 Phosphorylation
T279 Phosphorylation
S305 Phosphorylation
R307 Methylation
S313 Phosphorylation
S317 Phosphorylation
S324 Phosphorylation
T409 Phosphorylation
S411 Phosphorylation
S421 Phosphorylation
S594 Phosphorylation

Research Backgrounds

Function:

Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of embryonic neurogenesis. Also required postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. Negative regulator of NF-kappa-B signaling pathway. The inhibition of NF-kappa-B activation is mediated at least in part, by preventing MAP3K7IP2 to interact with polyubiquitin chains of TRAF6 and RIPK1 and by stimulating the 'Lys-48'-linked polyubiquitination and degradation of TRAF6 in cortical neurons.

Subcellular Location:

Cytoplasm.
Note: Symmetrically distributed throughout the cytoplasm in non dividing neuroblasts of the CNS.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts (via PTB domain) with MAP3K7IP2 (via C-terminal). Interacts (via C-terminal) with TRAF6 (via TRAF domains). Associates with EPS15 and NOTCH1.

Family&Domains:

The PTB domain is necessary for the inhibition of MAP3K7IP2-mediated activation of NF-kappa-B.

Research Fields

· Environmental Information Processing > Signal transduction > Notch signaling pathway.   (View pathway)

References

1). miR-140 Attenuates the Progression of Early-Stage Osteoarthritis by Retarding Chondrocyte Senescence. Molecular Therapy-Nucleic Acids (PubMed: 31790972) [IF=8.8]

Application: WB    Species: Human    Sample: Cartilage

Figure 5. Bioinformatics Analyses of the Potential Mechanisms by which miR-140 Regulates Chondrocyte Senescence (A) The number of predicted target genes of hsa-miR-140-5p in the miRDB, miRmap, PicTar, TargetScan, and DIANA microT-CDS databases. (B) Gene Ontology (GO) enrichment analysis for the predicted target genes of hsa-miR-140-5p, showing only the top 10 items in each category; the detailed results are provided in Figure S4. (C) Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis for the predicted target genes of hsa-miR-140-5p (p < 0.05). (D and E) Experimental validation of the effect of miR-140 on the proteins encoded by the predicted target genes of hsa-miR-140-5p by western blotting, including JAG1 and NUMBL in the Notch pathway (D) and IGF1R and TLR4 in the PI3K-AKT pathway (E). *p < 0.05, **p < 0.01.

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