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  • Product Name
    E-cadherin Antibody
  • Catalog No.
    AF0131
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC,ELISA
  • Reactivity
    Hm,Ms,Rt
  • UniProt
  • Mol.Wt.
    120kDa
  • Concentration
    1mg/ml
  • Browse similar products>>

Product Information

Alternative Names:Expand▼

Arc 1; CADH1_HUMAN; Cadherin 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD 324; CD324; CD324 antigen; cdh1; CDHE; E-Cad/CTF3; E-cadherin; ECAD; Epithelial cadherin; epithelial calcium dependant adhesion protein; LCAM; Liver cell adhesion molecule; UVO; Uvomorulin;

Applications:

WB: 1:500~1:3000 IHC: 1:50~1:200 IF/ICC: 1:100~1:500, ELISA(peptide) 1:20000-1:40000

Reactivity:

Human,Mouse,Rat

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

E-cadherin antibody detects endogenous levels of total E-cadherin.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human E-cadherin.

Uniprot:



>>Visit The Human Protein Atlas

Gene id:

Molecular Weight:

Observed Mol.Wt.: 120kDa.
Predicted Mol.Wt.: 98kDa.

Subcellular Location:

Cell junction. Cell membrane. Endosome. Golgi apparatus > trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.

Tissue Specificity:

Non-neural epithelial tissues.

Description:

CDH1 a single-pass type I membrane protein, and calcium dependent cell adhesion proteins. It is a ligand for integrin alpha-E/beta-7, and it colocalizes with DLG7 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis.

Sequence:
        10         20         30         40         50
MGPWSRSLSA LLLLLQVSSW LCQEPEPCHP GFDAESYTFT VPRRHLERGR
60 70 80 90 100
VLGRVNFEDC TGRQRTAYFS LDTRFKVGTD GVITVKRPLR FHNPQIHFLV
110 120 130 140 150
YAWDSTYRKF STKVTLNTVG HHHRPPPHQA SVSGIQAELL TFPNSSPGLR
160 170 180 190 200
RQKRDWVIPP ISCPENEKGP FPKNLVQIKS NKDKEGKVFY SITGQGADTP
210 220 230 240 250
PVGVFIIERE TGWLKVTEPL DRERIATYTL FSHAVSSNGN AVEDPMEILI
260 270 280 290 300
TVTDQNDNKP EFTQEVFKGS VMEGALPGTS VMEVTATDAD DDVNTYNAAI
310 320 330 340 350
AYTILSQDPE LPDKNMFTIN RNTGVISVVT TGLDRESFPT YTLVVQAADL
360 370 380 390 400
QGEGLSTTAT AVITVTDTND NPPIFNPTTY KGQVPENEAN VVITTLKVTD
410 420 430 440 450
ADAPNTPAWE AVYTILNDDG GQFVVTTNPV NNDGILKTAK GLDFEAKQQY
460 470 480 490 500
ILHVAVTNVV PFEVSLTTST ATVTVDVLDV NEAPIFVPPE KRVEVSEDFG
510 520 530 540 550
VGQEITSYTA QEPDTFMEQK ITYRIWRDTA NWLEINPDTG AISTRAELDR
560 570 580 590 600
EDFEHVKNST YTALIIATDN GSPVATGTGT LLLILSDVND NAPIPEPRTI
610 620 630 640 650
FFCERNPKPQ VINIIDADLP PNTSPFTAEL THGASANWTI QYNDPTQESI
660 670 680 690 700
ILKPKMALEV GDYKINLKLM DNQNKDQVTT LEVSVCDCEG AAGVCRKAQP
710 720 730 740 750
VEAGLQIPAI LGILGGILAL LILILLLLLF LRRRAVVKEP LLPPEDDTRD
760 770 780 790 800
NVYYYDEEGG GEEDQDFDLS QLHRGLDARP EVTRNDVAPT LMSVPRYLPR
810 820 830 840 850
PANPDEIGNF IDENLKAADT DPTAPPYDSL LVFDYEGSGS EAASLSSLNS
860 870 880
SESDKDQDYD YLNEWGNRFK KLADMYGGGE DD

Background

Function:

Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.

Post-translational Modifications:

During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.N-glycosylation at Asn-637 is essential for expression, folding and trafficking.Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-754.

Subcellular Location:

Endosome;Golgi apparatus;Plasma membrane;

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

Homodimer; disulfide-linked (PubMed:11856755). Component of an E-cadherin/ catenin adhesion complex composed of at least E-cadherin/CDH1, beta-catenin/CTNNB1 or gamma-catenin/JUP, and potentially alpha-catenin/CTNNA1; the complex is located to adherens junctions (PubMed:16126725, PubMed:7982500). Interacts with the TRPV4 and CTNNB1 complex (By similarity). Interacts with CTNND1 (PubMed:15240885). The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex (By similarity). Alternatively, the CTNNA1-containing complex may be linked to F-actin by other proteins such as LIMA1 (By similarity). Interaction with PSEN1, cleaves CDH1 resulting in the disassociation of cadherin-based adherens junctions (CAJs) (PubMed:11226248, PubMed:16126725). Interacts with AJAP1 and DLGAP5 (PubMed:14699157, PubMed:14595118). Interacts with TBC1D2 (PubMed:20116244). Interacts with LIMA1 (PubMed:18093941). Interacts with CAV1. Interacts with PIP5K1C (PubMed:17261850). Interacts with RAB8B (By similarity). Interacts with RAPGEF2 (By similarity). Interacts with DDR1; this stabilizes CDH1 at the cell surface and inhibits its internalization (PubMed:20432435). Interacts with KLRG1 (PubMed:19604491).

Similarity:

Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.

Research Fields

Research Fields:

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.(View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Apelin signaling pathway.(View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).(View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.(View pathway)
· Human Diseases > Cancers: Specific types > Thyroid cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Bladder cancer.(View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Gastric cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Endometrial cancer.(View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.
· Human Diseases > Cancers: Specific types > Melanoma.(View pathway)
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

Western blot analysis of extracts from mouse brain, using E-cadherin Antibody.
E-cadherin, β-catenin, vimentin, MMP-2 and MMP-9 which are EMT-related proteins, were assessed in terms of expression levels. EMT-related transcription factors (Snail, Slug, Twist and ZEB1) were measured in A549/PTX and A549/DDP cells using western blot analysis.
Western blot analysis on mouse liver tissue lysate using E-cadherin Antibody
Western blot analysis on 293 cell lysate using E-cadherin Antibody,The lane on the left is treated with the antigen-specific peptide.
JAM-A induced B-lymphoma cell invasion. (A,B) Epithelial-mesenchymal transition (EMT) was observed in JAM-A-overexpressing DB cells (A) and in DLBCL patients with high JAM-A expression (B). (C,D) JAM-A-transfected DB cells acquired increased cell invasion (C), which was inhibited in JAM-A-ShRNA-transfected cells (D). Data in (A,C and D) are representative of three independent experiments.
IHC analysis.
AF0131 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF0131 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
E-cadherin for IHC in human HepG2,Provided by Tianjin University

Reference Citations:

1). Li H et al. MicroRNA-181a regulates epithelial-mesenchymal transition by targeting PTEN in drug-resistant lung adenocarcinoma cells. Int J Oncol 2015 Oct;47(4):1379-92 (PubMed: 26323677)

Application: WB 1/500-1/3000    Species: human;    Sample: A549 cells;

Figure 3. A549/DDP and A549/PTX cells showed molecular and morphological changes that were consistent with EMT. (A) microscopy at x200 magnification was used to assess cell morphology. The A549 cells (parental cells) had an epithelioid, rounded cobblestone appearance and there was limited formation of pseudopodia. A549/PTX and A549/DDP cells exhibited a spindle-shaped morphology and an increased formation of pseudopodia, indicating a loss of cell polarity. (B) E-cadherin, β-catenin, vimentin, MMP-2 and MMP-9 which are EMT-related proteins, were assessed in terms of expression levels. EMT-related transcription factors (Snail, Slug, Twist and ZEB1) were measured in A549/PTX and A549/DDP cells using western blot analysis. (C) The expression changes were confirmed at the mRNA level by qRT-PCR. Expression was standardized to the expression of GAPDH and normalized to 1.0 in the parental cells (compared with the parental A549 cells, means ± SEM, n=3, * P<0.05)


2). Xiang XJ et al. MiR-1271 Inhibits Cell Proliferation, Invasion and EMT in Gastric Cancer by Targeting FOXQ1. Cell Physiol Biochem 2015;36(4):1382-94 (PubMed: 26159618)

Application: WB    Species: human;    Sample: human gastric cancer;

Fig. 6. MiR-1271 inhibited gastric cancer cell migration, invasion, and epithelial-mesenchymal transition. (A) Overexpression of miR-1271 could inhibit MGC-803 cell migration and invasion, whereas its downregulation in SGC-7901 cells increased the cell migration and invasion processes. (B) Western blots showed that overexpression of miR-1271 could upregulate E-cadherin and downregulate N-cadherin and vimentin expression in MGC-803 cells, whereas its downregulation had the opposite effect in SGC- 7901 cells. Three independent experiments were conducted. *P < 0.05, **P < 0.01.


3). Zhang J et al. FOXQ1 promotes gastric cancer metastasis through upregulation of Snail. Oncol Rep 2016 Jun;35(6):3607-13 (PubMed: 27109028)

Application: WB    Species: human;    Sample: BGC-823 cell;

Figure 4. Overexpression of FOXQ1 in BGC-823 cell line resulted in EMT and increased invasiveness. To determine whether FOXQ1 promotes the EMT to increase cell invasion, the expression levels of FOXQ1, E-cadherin and vimentin were detected via western blotting and qRT-PCR. The results revealed that FOXQ1 could increase EMT, which decreased E-cadherin expression and increased vimentin expression (* P


4). Bai Y et al. BCL2L10 inhibits growth and metastasis of hepatocellular carcinoma both in vitro and in vivo. Mol Carcinog 2017 Mar;56(3):1137-1149 (PubMed: 27770580)

Application: WB    Species: human;    Sample: HepG2;

Figure 6. Effect of BCL2L10 on its downstream gene expression profiles of human cancer pathway in HepG2 cells. (A) By human cancer pathway PCR array, ectopic expression of BCL2L10 up- or down-regulated several genes related to tumor proliferation, apoptosis, metastasis and angiogenesis. (B) Western blot was performed to confirm the downstream gene expression regulated by BCL2L10 in HepG2 cells. GAPDH was used as an internal control. (C) Schematic diagram of the molecular events for BCL2L10 function as a tumor suppressor through regulating cell cycle, proliferation, apoptosis metastasis and angiogenesis effectors.


5). Wang J et al. Knockdown of Nucleostemin in an ovarian cancer SKOV-3 cell line and its effects on cell malignancy. Biochem Biophys Res Commun 2017 May 27;487(2):262-267 (PubMed: 28412352)

Application: WB    Species: human;    Sample:Not available;

Fig. 3. Knockdown of NS inhibited tumor migration and invasion in vitro. (A) Crystal violet staining of the shNS and control group cells that crossed the polycarbonate membrane of the Transwell chamber to detect cell migration. (B) The number of cells that crossed the Transwell migration chamber in different groups. (C) Crystal violet staining of the shNS and control group cells that crossed the Matrigel-coated polycarbonate membrane of the Transwell chamber to detect cell invasion. (D) The number of cells that crossed the Transwell invasion chamber in different groups. (E) Representative Western blotting results indicate the EMT marker expressions in the different groups. The results are presented as the means ± SD, as based on three independent experiments. Statistical significance was determined using Student's t-test. *P < 0.05. Scale: 100 mm.


6). Yang J et al. miR-200b-containing microvesicles attenuate experimental colitis associated intestinal fibrosis by inhibiting epithelial-mesenchymal transition. J Gastroenterol Hepatol 2017 Dec;32(12):1966-1974 (PubMed: 28370348)

Application: WB    Species: rat;    Sample:Not available;

Effects of null-MVs and miR-200b-MVs administration on protein expression in TGFb-Indeced EMT.


7). Xu PP et al. JAM-A overexpression is related to disease progression in diffuse large B-cell lymphoma and downregulated by lenalidomide. Sci Rep 2017 Aug 7;7(1):7433 (PubMed: 28785100)

Application: IF/ICC    Species: mouse;    Sample:Not available;

Lenalidomide (1μM) inhibited JAM-A-transfected cell invasion (B) and EMT (C).


8). Guo J et al. Tumor-associated macrophages induce the expression of FOXQ1 to promote epithelial-mesenchymal transition and metastasis in gastric cancer cells. Oncol Rep 2017 Oct;38(4):2003-2010 (PubMed: 28791370)

Application: WB    Species: human;    Sample:Not available;

Figure 2. Co-culture with TAMs induces EMT in GC cells. (A) The EMT markers in MKN45 and MKN74 cells were analyzed using western blotting after being co-cultured with THP-1 cells. (B and C) The EMT markers in MKN45 and MKN74 cells were analyzed by RT-PCR after being co-cultured with THP-1 cells; * P


9). Li L et al. MiR-93-5p promotes gastric cancer-cell progression via inactivation of the Hippo signaling pathway. Gene 2018 Jan 30;641:240-247 (PubMed: 29045821)

Application: WB 1/600    Species: human;    Sample:Not available;

(C) miR-93-5p overexpression suppressed and increased E-cadherin and N-cadherin expression, respectively. The opposite result was observed in response to miR-93-5p downregulation.


10). Shi S et al. CRTC2 promotes non-small cell lung cancer A549 migration and invasion in vitro. Thorac Cancer 2018 Jan;9(1):136-141 (PubMed: 29105369)

11). Shao Q et al. MicroRNA-139-5p affects cisplatin sensitivity in human nasopharyngeal carcinoma cells by regulating the epithelial-to-mesenchymal transition. Gene 2018 Apr 30;652:48-58 (PubMed: 29427737)

12). Huang YX et al. Ovostatin 2 knockdown significantly inhibits the growth, migration, and tumorigenicity of cutaneous malignant melanoma cells. PLoS One 2018 Apr 23;13(4):e0195610 (PubMed: 29684087)

13). Qi F et al. Cinobufacini inhibits epithelial-mesenchymal transition of human hepatocellular carcinoma cells through c-Met/ERK signaling pathway. Biosci Trends 2018 Jul 17;12(3):291-297 (PubMed: 29794405)

14). Ran H et al. Stearoyl-CoA desaturase-1 promotes colorectal cancer metastasis in response to glucose by suppressing PTEN. J Exp Clin Cancer Res 2018 Mar 12;37(1):54 (PubMed: 29530061)

15). Meng J et al. Twist1 Regulates Vimentin through Cul2 Circular RNA to Promote EMT in Hepatocellular Carcinoma. Cancer Res 2018 Aug 1;78(15):4150-4162 (PubMed: 29844124)

16). Wu J et al. High glucose induces epithelial-mesenchymal transition and results in the migration and invasion of colorectal cancer cells. Exp Ther Med 2018 Jul;16(1):222-230 (PubMed: 29896243)

17). Ran H et al. Stearoyl-CoA desaturase-1 promotes colorectal cancer metastasis in response to glucose by suppressing PTEN. J Exp Clin Cancer Res 2018 Mar 12;37(1):54 (PubMed: 29530061)

18). Li XH et al. Parthenolide attenuated bleomycin-induced pulmonary fibrosis via the NF-κB/Snail signaling pathway. Respir Res 2018 Jun 5;19(1):111 (PubMed: 29871641)

19). Shi S et al. CRTC2 promotes non-small cell lung cancer A549 migration and invasion in vitro. Thorac Cancer 2018 Jan;9(1):136-141 (PubMed: 29105369)

20). Jin M et al. Responses of Intestinal Mucosal Barrier Functions of Rats to Simulated Weightlessness. Front Physiol 2018 Jun 14;9:729 (PubMed: 29962963)

21). Jia Y et al. CEP55 promotes the proliferation, migration and invasion of esophageal squamous cell carcinoma via the PI3K/Akt pathway. Onco Targets Ther 2018 Jul 20;11:4221-4232 (PubMed: 30050313)

22). Liu YR et al. Selenium-lentinan inhibits tumor progression by regulating epithelial-mesenchymal transition. Toxicol Appl Pharmacol 2018 Sep 18 (PubMed: 30240696)

23). Xin Z et al. Blocking FGFR4 exerts distinct anti-tumorigenic effects in esophageal squamous cell carcinoma. Thorac Cancer 2018 Sep 28 (PubMed: 30267473)

24). Wang H et al. Oleanolic acid inhibits epithelial-mesenchymal transition of hepatocellular carcinoma by promoting iNOS dimerization. Mol Cancer Ther 2018 Oct 8 (PubMed: 30297361)

25). Qin Y et al. Salidroside improves the hypoxic tumor microenvironment and reverses the drug resistance of platinum drugs via HIF-1α signaling pathway. EBioMedicine 2018 Nov 2 (PubMed: 30396856)

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Catalog Number :

AF0131-BP

Price/Size :

$200/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.