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E-cadherin Antibody AF0131

  • E-cadherin Antibody
  • E-cadherin Antibody
  • E-cadherin Antibody
  • E-cadherin Antibody
  • E-cadherin Antibody
  • E-cadherin Antibody
  • E-cadherin Antibody
  • E-cadherin Antibody
  • E-cadherin Antibody
  • E-cadherin Antibody
  • E-cadherin Antibody
  • E-cadherin Antibody
  • E-cadherin Antibody
  • E-cadherin Antibody
  • E-cadherin Antibody
  • E-cadherin Antibody
  • E-cadherin Antibody
  • E-cadherin Antibody
E-cadherin Antibody
  • Catalog No.
    AF0131
  • Source
    Rabbit
  • P12830 UniProt
  • 999 Gene ID
  • Predicted M.W.
    98kDa
  • Observed M.W.
    120kDa
  • Concentration
    1mg/ml
  • Clonality
    Polyclonal
  • Price/Size
    280$/100ul,350$/200ul
  • Delivery time
    Same day delivery

Browsing history

Datasheet Validation(9) Citations(22) Review Blocking Peptides

1). Li H et al. MicroRNA-181a regulates epithelial-mesenchymal transition by targeting PTEN in drug-resistant lung adenocarcinoma cells. Int J Oncol 2015 Oct;47(4):1379-92 (PubMed: 26323677)

Application: WB(1/500-1/3000);   Species: human;    Sample: A549 cells;

WB


2). Xiang XJ et al. MiR-1271 Inhibits Cell Proliferation, Invasion and EMT in Gastric Cancer by Targeting FOXQ1. Cell Physiol Biochem 2015;36(4):1382-94 (PubMed: 26159618)

Application: WB;   Species: human;    Sample: human gastric cancer;

WB


3). Zhang J et al. FOXQ1 promotes gastric cancer metastasis through upregulation of Snail. Oncol Rep 2016 Jun;35(6):3607-13 (PubMed: 27109028)

Application: WB;   Species: human;    Sample: BGC-823 cell;

WB


4). Bai Y et al. BCL2L10 inhibits growth and metastasis of hepatocellular carcinoma both in vitro and in vivo. Mol Carcinog 2017 Mar;56(3):1137-1149 (PubMed: 27770580)

Application: WB;   Species: human;    Sample: HepG2;

WB


5). Wang J et al. Knockdown of Nucleostemin in an ovarian cancer SKOV-3 cell line and its effects on cell malignancy. Biochem Biophys Res Commun 2017 May 27;487(2):262-267 (PubMed: 28412352)

Application: WB;   Species: human;    Sample:None Available;

WB


6). Yang J et al. miR-200b-containing microvesicles attenuate experimental colitis associated intestinal fibrosis by inhibiting epithelial-mesenchymal transition. J Gastroenterol Hepatol 2017 Dec;32(12):1966-1974 (PubMed: 28370348)

Application: WB;   Species: rat;    Sample:None Available;

WB


7). Xu PP et al. JAM-A overexpression is related to disease progression in diffuse large B-cell lymphoma and downregulated by lenalidomide. Sci Rep 2017 Aug 7;7(1):7433 (PubMed: 28785100)

Application: IF/ICC;   Species: mouse;    Sample:None Available;

IF/ICC


8). Guo J et al. Tumor-associated macrophages induce the expression of FOXQ1 to promote epithelial-mesenchymal transition and metastasis in gastric cancer cells. Oncol Rep 2017 Oct;38(4):2003-2010 (PubMed: 28791370)

Application: WB;   Species: human;    Sample:None Available;

WB


9). Li L et al. MiR-93-5p promotes gastric cancer-cell progression via inactivation of the Hippo signaling pathway. Gene 2018 Jan 30;641:240-247 (PubMed: 29045821)

Application: WB(1/600);   Species: human;    Sample:None Available;

WB


10). Shi S et al. CRTC2 promotes non-small cell lung cancer A549 migration and invasion in vitro. Thorac Cancer 2018 Jan;9(1):136-141 (PubMed: 29105369)

Application: None Available;   Species:None Available;    Sample:None Available;



11). Shao Q et al. MicroRNA-139-5p affects cisplatin sensitivity in human nasopharyngeal carcinoma cells by regulating the epithelial-to-mesenchymal transition. Gene 2018 Apr 30;652:48-58 (PubMed: 29427737)

Application: None Available;   Species:None Available;    Sample:None Available;



12). Huang YX et al. Ovostatin 2 knockdown significantly inhibits the growth, migration, and tumorigenicity of cutaneous malignant melanoma cells. PLoS One 2018 Apr 23;13(4):e0195610 (PubMed: 29684087)

Application: None Available;   Species:None Available;    Sample:None Available;



13). Qi F et al. Cinobufacini inhibits epithelial-mesenchymal transition of human hepatocellular carcinoma cells through c-Met/ERK signaling pathway. Biosci Trends 2018 Jul 17;12(3):291-297 (PubMed: 29794405)

Application: None Available;   Species:None Available;    Sample:None Available;



14). Ran H et al. Stearoyl-CoA desaturase-1 promotes colorectal cancer metastasis in response to glucose by suppressing PTEN. J Exp Clin Cancer Res 2018 Mar 12;37(1):54 (PubMed: 29530061)

Application: None Available;   Species:None Available;    Sample:None Available;



15). Meng J et al. Twist1 Regulates Vimentin through Cul2 Circular RNA to Promote EMT in Hepatocellular Carcinoma. Cancer Res 2018 Aug 1;78(15):4150-4162 (PubMed: 29844124)

Application: None Available;   Species:None Available;    Sample:None Available;



16). Wu J et al. High glucose induces epithelial-mesenchymal transition and results in the migration and invasion of colorectal cancer cells. Exp Ther Med 2018 Jul;16(1):222-230 (PubMed: 29896243)

Application: None Available;   Species:None Available;    Sample:None Available;



17). Ran H et al. Stearoyl-CoA desaturase-1 promotes colorectal cancer metastasis in response to glucose by suppressing PTEN. J Exp Clin Cancer Res 2018 Mar 12;37(1):54 (PubMed: 29530061)

Application: None Available;   Species:None Available;    Sample:None Available;



18). Li XH et al. Parthenolide attenuated bleomycin-induced pulmonary fibrosis via the NF-κB/Snail signaling pathway. Respir Res 2018 Jun 5;19(1):111 (PubMed: 29871641)

Application: None Available;   Species:None Available;    Sample:None Available;



19). Shi S et al. CRTC2 promotes non-small cell lung cancer A549 migration and invasion in vitro. Thorac Cancer 2018 Jan;9(1):136-141 (PubMed: 29105369)

Application: None Available;   Species:None Available;    Sample:None Available;



20). Jin M et al. Responses of Intestinal Mucosal Barrier Functions of Rats to Simulated Weightlessness. Front Physiol 2018 Jun 14;9:729 (PubMed: 29962963)

Application: None Available;   Species:None Available;    Sample:None Available;



21). Jia Y et al. CEP55 promotes the proliferation, migration and invasion of esophageal squamous cell carcinoma via the PI3K/Akt pathway. Onco Targets Ther 2018 Jul 20;11:4221-4232 (PubMed: 30050313)

Application: None Available;   Species:None Available;    Sample:None Available;



22). Liu YR et al. Selenium-lentinan inhibits tumor progression by regulating epithelial-mesenchymal transition. Toxicol Appl Pharmacol 2018 Sep 18 (PubMed: 30240696)

Application: None Available;   Species:None Available;    Sample:None Available;



Expand↓

Alternative Names:

Arc 1; CADH1_HUMAN; Cadherin 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD 324; CD324; CD324 antigen; cdh1; CDHE; E-Cad/CTF3; E-cadherin; ECAD; Epithelial cadherin; epithelial calcium dependant adhesion protein; LCAM; Liver cell adhesion molecule; UVO; Uvomorulin;

Applications:

WB: 1:500~1:3000 IHC: 1:50~1:200 IF/ICC: 1:100~1:500, ELISA(peptide) 1:20000-1:40000

Source:

Rabbit

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Concentration:

1mg/ml

Molecular Weight:

Observed: 120kDa. Predicted: 98kDa.

E-cadherin Antibody Western blot analysis on 293 cell lysate using E-cadherin Antibody,The lane on the left is treated with the antigen-specific peptide. Western blot analysis on 293 cell lysate using E-cadherin Antibody,The lane on the left is treated with the antigen-specific peptide.
E-cadherin Antibody E-cadherin for IHC in human HepG2,Provided by Tianjin University E-cadherin for IHC in human HepG2,Provided by Tianjin University
E-cadherin Antibody Western blot analysis on mouse liver  tissue  lysate using E-cadherin Antibody Western blot analysis on mouse liver tissue lysate using E-cadherin Antibody
E-cadherin Antibody  E-cadherin, β-catenin, vimentin, MMP-2 and MMP-9 which are EMT-related proteins, were assessed in terms of expression levels. EMT-related transcription factors (Snail, Slug, Twist and ZEB1) were measured in A549/PTX and A549/DDP cells using western blot analysis. E-cadherin, β-catenin, vimentin, MMP-2 and MMP-9 which are EMT-related proteins, were assessed in terms of expression levels. EMT-related transcription factors (Snail, Slug, Twist and ZEB1) were measured in A549/PTX and A549/DDP cells using western blot analysis.
E-cadherin Antibody AF0131 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary. AF0131 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
E-cadherin Antibody AF0131 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary. AF0131 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
E-cadherin Antibody IHC analysis. IHC analysis.
E-cadherin Antibody JAM-A induced B-lymphoma cell invasion. (A,B) Epithelial-mesenchymal transition (EMT) was observed in JAM-A-overexpressing DB cells (A) and in DLBCL patients with high JAM-A expression (B). (C,D) JAM-A-transfected DB cells acquired increased cell invasion (C), which was inhibited in JAM-A-ShRNA-transfected cells (D). Data in (A,C and D) are representative of three independent experiments. JAM-A induced B-lymphoma cell invasion. (A,B) Epithelial-mesenchymal transition (EMT) was observed in JAM-A-overexpressing DB cells (A) and in DLBCL patients with high JAM-A expression (B). (C,D) JAM-A-transfected DB cells acquired increased cell invasion (C), which was inhibited in JAM-A-ShRNA-transfected cells (D). Data in (A,C and D) are representative of three independent experiments.
E-cadherin Antibody Western blot analysis of extracts from mouse brain, using E-cadherin Antibody. Western blot analysis of extracts from mouse brain, using E-cadherin Antibody.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

E-cadherin antibody detects endogenous levels of total E-cadherin.

Immunogen:

A synthesized peptide derived from human E-cadherin.

Uniprot:

Gene id:

Show more↓

Research Fields:

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.(View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Apelin signaling pathway.(View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).(View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.(View pathway)
· Human Diseases > Cancers: Specific types > Thyroid cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Bladder cancer.(View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Gastric cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Endometrial cancer.(View pathway)

Description:

CDH1 a single-pass type I membrane protein, and calcium dependent cell adhesion proteins. It is a ligand for integrin alpha-E/beta-7, and it colocalizes with DLG7 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis.

Function:

Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.

Subcellular Location:

Cell junction. Cell membrane. Endosome. Golgi apparatus > trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.

Post-translational Modifications:

During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.N-glycosylation at Asn-637 is essential for expression, folding and trafficking.Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-754.

Tissue Specificity:

Non-neural epithelial tissues.

Subunit Structure:

Homodimer; disulfide-linked (PubMed:11856755). Component of an E-cadherin/ catenin adhesion complex composed of at least E-cadherin/CDH1, beta-catenin/CTNNB1 or gamma-catenin/JUP, and potentially alpha-catenin/CTNNA1; the complex is located to adherens junctions (PubMed:16126725, PubMed:7982500). Interacts with the TRPV4 and CTNNB1 complex (By similarity). Interacts with CTNND1 (PubMed:15240885). The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex (By similarity). Alternatively, the CTNNA1-containing complex may be linked to F-actin by other proteins such as LIMA1 (By similarity). Interaction with PSEN1, cleaves CDH1 resulting in the disassociation of cadherin-based adherens junctions (CAJs) (PubMed:11226248, PubMed:16126725). Interacts with AJAP1 and DLGAP5 (PubMed:14699157, PubMed:14595118). Interacts with TBC1D2 (PubMed:20116244). Interacts with LIMA1 (PubMed:18093941). Interacts with CAV1. Interacts with PIP5K1C (PubMed:17261850). Interacts with RAB8B (By similarity). Interacts with RAPGEF2 (By similarity). Interacts with DDR1; this stabilizes CDH1 at the cell surface and inhibits its internalization (PubMed:20432435). Interacts with KLRG1 (PubMed:19604491).

Similarity:

Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.

Protein BLAST With

Sequence:

        10         20         30         40         50
MGPWSRSLSA LLLLLQVSSW LCQEPEPCHP GFDAESYTFT VPRRHLERGR
60 70 80 90 100
VLGRVNFEDC TGRQRTAYFS LDTRFKVGTD GVITVKRPLR FHNPQIHFLV
110 120 130 140 150
YAWDSTYRKF STKVTLNTVG HHHRPPPHQA SVSGIQAELL TFPNSSPGLR
160 170 180 190 200
RQKRDWVIPP ISCPENEKGP FPKNLVQIKS NKDKEGKVFY SITGQGADTP
210 220 230 240 250
PVGVFIIERE TGWLKVTEPL DRERIATYTL FSHAVSSNGN AVEDPMEILI
260 270 280 290 300
TVTDQNDNKP EFTQEVFKGS VMEGALPGTS VMEVTATDAD DDVNTYNAAI
310 320 330 340 350
AYTILSQDPE LPDKNMFTIN RNTGVISVVT TGLDRESFPT YTLVVQAADL
360 370 380 390 400
QGEGLSTTAT AVITVTDTND NPPIFNPTTY KGQVPENEAN VVITTLKVTD
410 420 430 440 450
ADAPNTPAWE AVYTILNDDG GQFVVTTNPV NNDGILKTAK GLDFEAKQQY
460 470 480 490 500
ILHVAVTNVV PFEVSLTTST ATVTVDVLDV NEAPIFVPPE KRVEVSEDFG
510 520 530 540 550
VGQEITSYTA QEPDTFMEQK ITYRIWRDTA NWLEINPDTG AISTRAELDR
560 570 580 590 600
EDFEHVKNST YTALIIATDN GSPVATGTGT LLLILSDVND NAPIPEPRTI
610 620 630 640 650
FFCERNPKPQ VINIIDADLP PNTSPFTAEL THGASANWTI QYNDPTQESI
660 670 680 690 700
ILKPKMALEV GDYKINLKLM DNQNKDQVTT LEVSVCDCEG AAGVCRKAQP
710 720 730 740 750
VEAGLQIPAI LGILGGILAL LILILLLLLF LRRRAVVKEP LLPPEDDTRD
760 770 780 790 800
NVYYYDEEGG GEEDQDFDLS QLHRGLDARP EVTRNDVAPT LMSVPRYLPR
810 820 830 840 850
PANPDEIGNF IDENLKAADT DPTAPPYDSL LVFDYEGSGS EAASLSSLNS
860 870 880
SESDKDQDYD YLNEWGNRFK KLADMYGGGE DD

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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Catalog Number :

AF0131-BP

Price/Size :

$200/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

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