PPP1R2 Antibody - #AF0799

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Product: PPP1R2 Antibody
Catalog: AF0799
Description: Rabbit polyclonal antibody to PPP1R2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 25kDa; 23kD(Calculated).
Uniprot: P41236
RRID: AB_2833792

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
PPP1R2 Antibody detects endogenous levels of total PPP1R2.
RRID:
AB_2833792
Cite Format: Affinity Biosciences Cat# AF0799, RRID:AB_2833792.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

IPP 2; IPP-2; IPP2; IPP2_HUMAN; MGC87148; Phosphatase inhibitor 2; Phosphoprotein phosphatase; Ppp1r2; PPP1R2 protein; Protein phosphatase 1 regulatory (inhibitor) subunit 2; Protein phosphatase 1 regulatory subunit 2; Protein phosphatase inhibitor 2;

Immunogens

Immunogen:
Uniprot:

P41236(IPP2_HUMAN) >>Visit HPA database.

Gene(ID):
PPP1R2(5504)
Description:
PPP1R2 a member of the protein phosphatase inhibitor 1 family. A dopamine- and cyclic AMP-regulated neuronal phosphoprotein. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32; phosphorylated DARPP32 is a potent protein phosphatase-1 inhibitor. NMDA receptor stimulation elevates intracellular calcium, which leads to activation of calcineurin and dephosphorylation of phospho-DARPP32, thereby reducing the phosphatase-1 inhibitory activity of DARPP32.
Sequence:
MAASTASHRPIKGILKNKTSTTSSMVASAEQPRGNVDEELSKKSQKWDEMNILATYHPADKDYGLMKIDEPSTPYHSMMGDDEDACSDTEATEAMAPDILARKLAAAEGLEPKYRIQEQESSGEEDSDLSPEEREKKRQFEMKRKLHYNEGLNIKLARQLISKDLHDDDEDEEMLETADGESMNTEESNQGSTPSDQQQNKLRSS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P41236 As Substrate

Site PTM Type Enzyme
S4 Phosphorylation
S7 Phosphorylation
T19 Phosphorylation
S20 Phosphorylation
S23 Phosphorylation
S24 Phosphorylation
S28 Phosphorylation
R33 Methylation
S44 Phosphorylation Q13315 (ATM)
T55 Phosphorylation
Y56 Phosphorylation
Y63 Phosphorylation
S72 Phosphorylation P27448 (MARK3)
T73 Phosphorylation P49841 (GSK3B) , Q00535 (CDK5)
Y75 Phosphorylation
S77 Phosphorylation
S87 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2) , O94921 (CDK14)
T89 Phosphorylation
T92 Phosphorylation
K103 Ubiquitination
K113 Ubiquitination
Y114 Phosphorylation
S121 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
S122 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
S127 Phosphorylation
S130 Phosphorylation
K145 Ubiquitination
Y148 Phosphorylation
K155 Ubiquitination
T193 Phosphorylation

Research Backgrounds

Function:

Inhibitor of protein-phosphatase 1.

PTMs:

Phosphorylation on Thr-73 by GSK3 activates PP1 by dissociating the PP1-PPP1R2 complex (By similarity). Phosphorylation on Ser-44 by ATM activates PP1 by dissociating the PP1-PPP1R2 complex.

Subunit Structure:

Heterodimer with PP1.

Family&Domains:

Belongs to the protein phosphatase inhibitor 2 family.

Restrictive clause

 

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For Research Use Only.
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