Osteopontin Antibody - #DF6395

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Product: Osteopontin Antibody
Catalog: DF6395
Description: Rabbit polyclonal antibody to Osteopontin
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Sheep
Mol.Wt.: 33kDa; 35kD(Calculated).
Uniprot: P10451
RRID: AB_2838358

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Sheep(80%)
Clonality:
Polyclonal
Specificity:
Osteopontin Antibody detects endogenous levels of total Osteopontin.
RRID:
AB_2838358
Cite Format: Affinity Biosciences Cat# DF6395, RRID:AB_2838358.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BNSP; Bone sialoprotein 1; BSP I; BSPI; Early T lymphocyte activation 1; ETA 1; ETA1; MGC110940; Nephropontin; OPN; Osteopontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; OSTP_HUMAN; PSEC0156; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); Secreted phosphoprotein 1; SPP 1; SPP-1; SPP1; SPP1/CALPHA1 fusion; Urinary stone protein; Uropontin;

Immunogens

Immunogen:
Uniprot:

P10451(OSTP_HUMAN) >>Visit HPA database.

Gene(ID):
SPP1(6696)
Expression:
P10451 OSTP_HUMAN:

Bone. Found in plasma.

Description:
Osteopontin (OPN), also designated bone sialoprotein 1, urinary stone protein, spp-1, Eta-1, nephropontin and uropontin, is an extracellular matrix cell adhesion phosphoglycoprotein. OPN is deposited into unmineralized matrix prior to calcification leading to localization at various tissue interfaces including cement lines, lamina limitans and between collagen fibrils of fully matured hard tissues. While OPN is a major product of osteoblasts, it is also synthesized by brain and kidney cells. OPN isolated from or secreted by various tissues ranges in molecular weight due to posttranslational modifications. OPN functions as a substrate for transglutaminase and is involved in cell adhesion, chemoattraction and immunomodulation.
Sequence:
MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFKQETLPSKSNESHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQLDDQSAETHSHKQSRLYKRKANDESNEHSDVIDSQELSKVSREFHSHEFHSHEDMLVVDPKSKEEDKHLKFRISHELDSASSEVN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Sheep
80
Pig
0
Horse
0
Bovine
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P10451 As Substrate

Site PTM Type Enzyme
S24 Phosphorylation
S26 Phosphorylation
S27 Phosphorylation
S49 O-Glycosylation
S49 Phosphorylation
S62 Phosphorylation
S63 Phosphorylation
T66 Phosphorylation
S76 Phosphorylation
S78 Phosphorylation
S81 Phosphorylation
S99 Phosphorylation
S102 Phosphorylation
S105 Phosphorylation
S108 Phosphorylation
S117 Phosphorylation
S120 Phosphorylation
S123 Phosphorylation
S126 Phosphorylation
S129 Phosphorylation
T134 O-Glycosylation
T134 Phosphorylation
T138 O-Glycosylation
T138 Phosphorylation
T143 O-Glycosylation
T143 Phosphorylation
T147 O-Glycosylation
T147 Phosphorylation
T152 O-Glycosylation
T152 Phosphorylation
Y165 Phosphorylation
T185 Phosphorylation
T190 Phosphorylation
S191 Phosphorylation
S195 Phosphorylation
Y202 Phosphorylation
S215 Phosphorylation
S219 Phosphorylation
S224 Phosphorylation
Y225 Phosphorylation
T227 Phosphorylation
S228 Phosphorylation
S234 Phosphorylation
T237 Phosphorylation
S239 Phosphorylation
S243 Phosphorylation
S254 Phosphorylation
S258 Phosphorylation
S263 Phosphorylation
S267 Phosphorylation
S270 Phosphorylation
S275 Phosphorylation
S280 Phosphorylation
S291 Phosphorylation
S303 Phosphorylation
S308 Phosphorylation
S310 Phosphorylation
S311 Phosphorylation

Research Backgrounds

Function:

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.

Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

PTMs:

Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif.

O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Bone. Found in plasma.

Subunit Structure:

Ligand for integrin alpha-V/beta-3.

Family&Domains:

Belongs to the osteopontin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

References

1). Сравнительный анализ методик локальной стимуляции репаративного остеогенеза. Инновационная медицина Кубани
2). COMPARATIVE ANALYSIS OF LOCAL STIMULATION METHODS OF REPARATIVE OSTEOGENESIS. Инновационная медицина Кубани

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