SDC1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Hela cells(heat-shock treatment), using SDC1 Antibody.

Product:	SDC1 Antibody
Catalog:	DF6367
Description:	Rabbit polyclonal antibody to SDC1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	33kDa; 32kD(Calculated).
Uniprot:	P18827
RRID:	AB_2838331

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(83%), Xenopus(92%)

Clonality:

Polyclonal

Specificity:

SDC1 Antibody detects endogenous levels of total SDC1.

RRID:

AB_2838331
Cite Format: Affinity Biosciences Cat# DF6367, RRID:AB_2838331.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD_antigen; CD138; CD138 antigen; heparan sulfate proteoglycan fibroblast growth factor receptor; SDC; Sdc1; SDC1_HUMAN; SYND1; Syndecan 1; syndecan proteoglycan 1; Syndecan-1;

Immunogens

Immunogen:

Uniprot:

P18827(SDC1_HUMAN) >>Visit HPA database.

Gene(ID):

SDC1(6382)

Description:

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008]

Sequence:

P18827 SDC1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRRAALWLWLCALALSLQPALPQIVATNLPPEDQDGSGDDSDNFSGSGAGALQDITLSQQTPSTWKDTQLLTAIPTSPEPTGLEATAASTSTLPAGEGPKEGEAVVLPEVEPGLTAREQEATPRPRETTQLPTTHLASTTTATTAQEPATSHPHRDMQPGHHETSTPAGPSQADLHTPHTEDGGPSATERAAEDGASSQLPAAEGSGEQDFTFETSGENTAVVAVEPDRRNQSPVDQGATGASQGLLDRKEVLGGVIAGGLVGLIFAVCLVGFMLYRMKKKDEGSYSLEEPKQANGGAYQKPTKQEEFYA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Dog

100

Rabbit

100

Xenopus

Chicken

Sheep

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P18827 As Substrate

P18827

Site	PTM Type	Source
T61	O-Glycosylation	Uniprot
T72	O-Glycosylation	Uniprot
T76	O-Glycosylation	Uniprot
S77	O-Glycosylation	Uniprot
T81	O-Glycosylation	Uniprot
T122	O-Glycosylation	Uniprot
T164	O-Glycosylation	Uniprot
S165	O-Glycosylation	Uniprot
T166	O-Glycosylation	Uniprot
S171	O-Glycosylation	Uniprot
T177	O-Glycosylation	Uniprot
T180	O-Glycosylation	Uniprot
S186	O-Glycosylation	Uniprot
T188	O-Glycosylation	Uniprot
T240	O-Glycosylation	Uniprot
K280	Ubiquitination	Uniprot
K281	Ubiquitination	Uniprot
S285	Phosphorylation	Uniprot
Y286	Phosphorylation	Uniprot
S287	Phosphorylation	Uniprot
K292	Ubiquitination	Uniprot
Y299	Phosphorylation	Uniprot
K301	Ubiquitination	Uniprot
K304	Ubiquitination	Uniprot
Y309	Phosphorylation	Uniprot

Research Backgrounds

P18827_SDC1_HUMAN

Function:

Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP.

PTMs:

Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.

Subcellular Location:

Membrane>Single-pass type I membrane protein. Secreted. Secreted>Extracellular exosome.
Note: Shedding of the ectodomain produces a soluble form.

Subunit Structure:

Interacts with CDCP1. Interacts (via C-terminus) with TIAM1 (via PDZ domain). Interacts with MDK (By similarity).

Family&Domains:

Belongs to the syndecan proteoglycan family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Malaria.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

References

1). LncRNA H19 deficiency protects against the structural damage of glomerular endothelium in diabetic nephropathy via Akt/eNOS pathway. ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (PubMed: 35867533) [IF=3.0]

2). Repression of T Cell-Mediated Alloimmunity by CX-5461 via the 1 p53-DUSP5 Pathway.

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SDC1 Antibody - #DF6367