Caveolin 1 Antibody - #AF0126

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Product: Caveolin 1 Antibody
Catalog: AF0126
Description: Rabbit polyclonal antibody to Caveolin 1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Horse
Mol.Wt.: 25kDa; 20kD(Calculated).
Uniprot: Q03135
RRID: AB_2833310

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IF/ICC: 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(100%)
Clonality:
Polyclonal
Specificity:
Caveolin 1 Antibody detects endogenous levels of total Caveolin 1.
RRID:
AB_2833310
Cite Format: Affinity Biosciences Cat# AF0126, RRID:AB_2833310.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BSCL3; CAV; CAV1; CAV1_HUMAN; caveolae protein, 22 kD; caveolin 1 alpha isoform; caveolin 1 beta isoform; Caveolin 1 caveolae protein 22kDa; Caveolin-1; Caveolin1; cell growth-inhibiting protein 32; CGL3; LCCNS; MSTP085; OTTHUMP00000025031; PPH3; VIP 21; VIP21;

Immunogens

Immunogen:
Uniprot:

Q03135(CAV1_HUMAN) >>Visit HPA database.

Gene(ID):
CAV1(857)
Expression:
Q03135 CAV1_HUMAN:

Skeletal muscle, liver, stomach, lung, kidney and heart (at protein level). Expressed in the brain.

Description:
caveolin-1 May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Involved in the costimulatory signal essential for T-cell receptor (TCR)- mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3- dependent manner. Belongs to the caveolin family. Homooligomer. Interacts with GLIPR2, NOSTRIN, SNAP25 and syntaxin. Interacts with rotavirus A NSP4.
Sequence:
MSGGKYVDSEGHLYTVPIREQGNIYKPNNKAMADELSEKQVYDAHTKEIDLVNRDPKHLNDDVVKIDFEDVIAEPEGTHSFDGIWKASFTTFTVTKYWFYRLLSALFGIPMALIWGIYFAILSFLHIWAVVPCIKSFLIEIQCISRVYSIYVHTVCDPLFEAVGKIFSNVRINLQKEI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Pig
75
Bovine
75
Sheep
75
Dog
75
Rabbit
75
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q03135 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
K5 Acetylation
K5 Ubiquitination
Y6 Phosphorylation
S9 Phosphorylation
Y14 Phosphorylation P12931 (SRC) , P06241 (FYN) , P00519 (ABL1)
T15 Phosphorylation
Y25 Phosphorylation
K26 Sumoylation
K26 Ubiquitination
K30 Ubiquitination
S37 Phosphorylation
K39 Ubiquitination
Y42 Phosphorylation
T46 Phosphorylation
K47 Ubiquitination
K57 Acetylation
K57 Ubiquitination
K65 Ubiquitination
S80 Phosphorylation
S88 Phosphorylation
S168 Phosphorylation
K176 Ubiquitination

Research Backgrounds

Function:

May act as a scaffolding protein within caveolar membranes. Forms a stable heterooligomeric complex with CAV2 that targets to lipid rafts and drives caveolae formation. Mediates the recruitment of CAVIN proteins (CAVIN1/2/3/4) to the caveolae. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway (By similarity). Negatively regulates TGFB1-mediated activation of SMAD2/3 by mediating the internalization of TGFBR1 from membrane rafts leading to its subsequent degradation.

PTMs:

Ubiquitinated. Undergo monoubiquitination and multi- and/or polyubiquitination. Monoubiquitination of N-terminal lysines promotes integration in a ternary complex with UBXN6 and VCP which promotes oligomeric CAV1 targeting to lysosomes for degradation.

The initiator methionine for isoform 2 is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.

Phosphorylated at Tyr-14 by ABL1 in response to oxidative stress.

Subcellular Location:

Golgi apparatus membrane>Peripheral membrane protein. Cell membrane>Peripheral membrane protein. Membrane>Caveola>Peripheral membrane protein. Membrane raft. Golgi apparatus>trans-Golgi network.
Note: Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Skeletal muscle, liver, stomach, lung, kidney and heart (at protein level). Expressed in the brain.

Subunit Structure:

Homooligomer. Interacts with GLIPR2. Interacts with NOSTRIN. Interacts with SNAP25 and STX1A (By similarity). Interacts (via the N-terminus) with DPP4; the interaction is direct. Interacts with CTNNB1, CDH1 and JUP. Interacts with PACSIN2; this interaction induces membrane tubulation (By similarity). Interacts with SLC7A9 (By similarity). Interacts with BMX and BTK. Interacts with TGFBR1. Interacts with CAVIN3 (via leucine-zipper domain) in a cholesterol-sensitive manner. Interacts with CAVIN1. Interacts with EHD2 in a cholesterol-dependent manner. Forms a ternary complex with UBXN6 and VCP; mediates CAV1 targeting to lysosomes for degradation. Interacts with ABCG1; this interaction regulates ABCG1-mediated cholesterol efflux.

(Microbial infection) Interacts with rotavirus A NSP4.

Family&Domains:

Belongs to the caveolin family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cardiovascular diseases > Viral myocarditis.

References

1). Stem cell membrane-coated isotretinoin for acne treatment. JOURNAL OF NANOBIOTECHNOLOGY, 2020 (PubMed: 32723398) [IF=10.2]
2). Study on the Mechanism of Sancao Tiaowei Decoction in the Treatment of MNNG-Induced Precancerous Lesions of Gastric Carcinoma Through Hedgehog Signaling Pathway. Frontiers in Oncology, 2022 (PubMed: 35646631) [IF=4.7]
3). Identification of prothymosin alpha (PTMA) as a biomarker for esophageal squamous cell carcinoma (ESCC) by label-free quantitative proteomics and Quantitative Dot Blot (QDB). Clinical Proteomics, 2019 (PubMed: 30988666) [IF=3.8]

Application: WB    Species: human    Sample: ESCC tissues

Fig. 5|The diferentially expressed proteins were validated by Western Blot. Compared with adjacent normal tissues, the protein expression of PTMA, PAK2, PPP1CA, HMGB2 were up-regulated (a, b), and the protein expression of Caveolin, Integrin beta-1, Collagen alpha-2(VI), Leiomodin-1, Vinculin were down-regulated in ESCC tissues from four pairs of samples (c, d). Representative immunoblot images (a, c) and histograms (mean ± SD; b, d).The experiments were repeated at least three times, N represented normal tissues and T represented tumor tissues
4). O-GlcNAcylation of Keratin 18 coordinates TCA cycle to promote cholangiocarcinoma progression. bioRxiv, 2023

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