Ferritin Heavy Chain Antibody | Affinity Biosciences

Product:	FTH1 Antibody
Catalog:	DF6278
Description:	Rabbit polyclonal antibody to Ferritin Heavy Chain
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	21kDa; 21kD(Calculated).
Uniprot:	P02794
RRID:	AB_2838244

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Rabbit(90%), Dog(90%), Chicken(80%), Xenopus(88%)

Clonality:

Polyclonal

Specificity:

Ferritin Heavy Chain Antibody detects endogenous levels of total Ferritin Heavy Chain.

RRID:

AB_2838244
Cite Format: Affinity Biosciences Cat# DF6278, RRID:AB_2838244.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Apoferritin; Cell proliferation inducing gene 15 protein; Cell proliferation-inducing gene 15 protein; F HC; Ferritin H subunit; Ferritin heavy chain; Ferritin heavy polypeptide 1; FHC; FRIH; FRIH_HUMAN; FTH 1; FTH; FTH1; FTH1 protein; FTHL 6; FTHL6; Iron overload autosomal dominant; MGC104426; N-terminally processed; OK/SW-cl.84; PIG 15; PIG15; Placenta immunoregulatory factor; PLIF; Proliferation inducing gene 15 protein; Proliferation inducing protein 15;

Immunogens

Immunogen:

Uniprot:

P02794(FRIH_HUMAN) >>Visit HPA database.

Gene(ID):

FTH1(2495)

Expression:

P02794 FRIH_HUMAN:

Expressed in the liver.

Description:

Ferritin (FTH) is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis by sequestering and storing iron in a non-toxic and bioavailable form (1). The assembled ferritin molecule, often referred to as a nanocage, can store up to 4,500 atoms of iron (2,3). It forms a holoenzyme of ~450 kDa, consisting of 24 subunits made up of two types of polypeptide chains: ferritin heavy chain and ferritin light chain, each having unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe(II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe(III) (4). In addition to iron buffering, heavy chain ferritin also enhances thymidine biosynthesis (5). Serum ferritin levels serve as an indicator of the amount of iron stored in the body. Serum ferritin is the most sensitive test for anaemia. The level of serum ferritin is markedly elevated in inflammation, malignancy, and iron overload disorders (6). Research studies have found that defects in ferritin proteins are also associated with several neurodegenerative diseases (7).

Sequence:

P02794 FRIH_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTTASTSQVRQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSHEEREHAEKLMKLQNQRGGRIFLQDIKKPDCDDWESGLNAMECALHLEKNVNQSLLELHKLATDKNDPHLCDFIETHYLNEQVKAIKELGDHVTNLRKMGAPESGLAEYLFDKHTLGDSDNES

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Dog

Xenopus

Chicken

Horse

Pig

Bovine

Sheep

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P02794 As Substrate

P02794

Site	PTM Type	Source
M1	Acetylation	Uniprot
T2	Acetylation	Uniprot
T6	Phosphorylation	Uniprot
S7	Phosphorylation	Uniprot
Y13	Phosphorylation	Uniprot
K54	Ubiquitination	Uniprot
K69	Ubiquitination	Uniprot
K72	Sumoylation	Uniprot
K72	Ubiquitination	Uniprot
K88	Ubiquitination	Uniprot
S96	Phosphorylation	Uniprot
K109	Ubiquitination	Uniprot
S114	Phosphorylation	Uniprot
K120	Ubiquitination	Uniprot
K125	Ubiquitination	Uniprot
K144	Ubiquitination	Uniprot
K147	Ubiquitination	Uniprot
K158	Ubiquitination	Uniprot
S164	Phosphorylation	Uniprot
Y169	Phosphorylation	Uniprot
T175	Phosphorylation	Uniprot
S179	Phosphorylation	Uniprot
S183	Phosphorylation	Uniprot

Research Backgrounds

P02794_FRIH_HUMAN

Function:

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity).

Tissue Specificity:

Expressed in the liver.

Subunit Structure:

Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. In the human liver, the heavy chain is predominant. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited.

Family&Domains:

Belongs to the ferritin family.

Research Fields

· Cellular Processes > Cell growth and death > Ferroptosis. (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Organismal Systems > Digestive system > Mineral absorption.

References

1). VDR Activation Attenuates Renal Tubular Epithelial Cell Ferroptosis by Regulating Nrf2/HO-1 Signaling Pathway in Diabetic Nephropathy. Advanced science (Weinheim, Baden-Wurttemberg, Germany) (PubMed: 38145959) [IF=15.1]

2). Activation of integrated stress response and disordered iron homeostasis upon combined exposure to cadmium and PCB77. JOURNAL OF HAZARDOUS MATERIALS (PubMed: 31837937) [IF=13.6]

Application: WB Species: Human Sample: Human erythroleukemia cell lines (HEL)

Fig. 5. Disordered iron homeostasis and inhibited mTORC1 activity upon exposure to CdCl2 and PCB77 at low dose. (A) The relative fluorescence intensity of CAeAM for measuring LIP to reflect intracellular iron availability (n = 3–4), and (B) Representative blots of FTH1 protein content to reflect iron storage. Analyses were performed after single or combined exposure to CdCl2 and PCB77 at 1 μM for 48 h. (C) Phosphorylated S6 and total S6 content to re- flect mTORC1 activity as measured by Western blot. Ratio of FTH1 to eIF2αP and ratio of pS6 to S6 in the control group were defined as 1. Analyses were performed after single or combined exposure to CdCl2 and PCB77 at 1 μM for 48 h. a- significantly different from the control group. Data were presented in mean ± SE. P < 0.05 was considered statistically significant.

3). Identification of ZIP8-induced ferroptosis as a major type of cell death in monocytes under sepsis conditions. Redox biology (PubMed: 38103342) [IF=11.4]

4). Oxoeicosanoid receptor inhibition alleviates acute myocardial infarction through activation of BCAT1. BASIC RESEARCH IN CARDIOLOGY (PubMed: 33484341) [IF=9.5]

Application: IHC Species: mouse Sample: heart

Fig. 5| Overexpression of BCAT1 and BCAT2 ameliorated AMIinduced myocardial injury. All mice were killed 24 h after the left anterior descending CAL. The heart-specifc BCAT1 or BCAT2 over-expression mice were constructed by direct injecting Adenoassociated virus (AAV) vectors carrying BCAT1 or BCAT2 in the LV free wall in mice using a syringe with a 30-gauge needle.h Representative immunohistochemical analysis of the expression of FHC, FLC, GPX4 and Caspase-3 in heart tissue of sham and model mice with cardiac overexpression of BCAT1 and BCAT2 (n = 3 mice) (400 × magnifcation, scale bar, 50 µm). #p < 0.05, ##p < 0.01 versus sham group, *p < 0.05, **p < 0.01 versus model group

5). Suppression of the SLC7A11/glutathione axis causes ferroptosis and apoptosis and alters the mitogen-activated protein kinase pathway in nasopharyngeal carcinoma. International journal of biological macromolecules (PubMed: 37951442) [IF=8.2]

6). Tiliroside targets TBK1 to induce ferroptosis and sensitize hepatocellular carcinoma to sorafenib. Phytomedicine (PubMed: 36657316) [IF=7.9]

7). GSK-3β-dependent Nrf2 antioxidant response modulates ferroptosis of lens epithelial cells in age-related cataract. Free Radical Biology and Medicine (PubMed: 37156294) [IF=7.4]

8). YAP1 alleviates sepsis-induced acute lung injury via inhibiting ferritinophagy-mediated ferroptosis. Frontiers in Immunology (PubMed: 35979359) [IF=7.3]

9). YAP1 protects against PM2.5-induced lung toxicity by suppressing pyroptosis and ferroptosis. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (PubMed: 36863160) [IF=6.8]

Application: WB Species: Mouse Sample: lung tissues

Fig. 3. YAP1 knockout accelerates ferroptosis in PM2.5-induced lung toxicity. A, Representative fluorescent images of ROS staining; B, Quantification of ROS labeling intensity in the lung; C, Representative TEM images. The red arrow indicates representative mitochondria in WT or YAP1-CKO mouse lungs exposed to PM2.5 or not; D, Immunoblotting detection of SLC7A11, GPX4 and FTH1 expression in mice with the indicated treatment; quantitative results are shown in E-G n = 5; lung content of Fe2+ (H), MDA (I) and GSH (J) measured by the corresponding commercial kit, n = 5. The values are shown as the mean ± SD,

10). Alleviated NCOA4-mediated ferritinophagy protected RA FLSs from ferroptosis in lipopolysaccharide-induced inflammation under hypoxia. Inflammation research : official journal of the European Histamine Research Society ... [et al.] (PubMed: 38189810) [IF=6.7]

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FTH1 Antibody - #DF6278