Price Size
$250 50ul
$350 100ul
$450 200ul

Ship 3-4 business days

Contact distributor
  • Product Name
    ABL2 Antibody
  • Catalog No.
    BF0255
  • RRID
    AB_2833755
  • Source
    Mouse
  • Application
    WB,IF/ICC,ELISA
  • Reactivity
    Human, Mouse
  • UniProt
  • Mol.Wt
    128kD;
    128kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

Product Information

Alternative Names:Expand▼

Abelson murine leukemia viral oncogene homolog 2; Abelson related gene protein; Abelson tyrosine-protein kinase 2; Abelson-related gene protein; ABL2; ABL2_HUMAN; ABLL; ARG; Tyrosine kinase ARG; Tyrosine protein kinase ABL2; Tyrosine-protein kinase ARG; v abl Abelson murine leukemia viral oncogene homolog 2;

Applications:

ELISA 1:10000, WB 1:500-1:2000, IF/ICC 1:200-1:1000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse

Source:

Mouse

Clonality:

Monoclonal

Clone Number:

AFB1731

Purification:

Affinity-chromatography.

Specificity:

ABL2 antibody detects endogenous levels of total ABL2.

RRID:

AB_2833755
Please cite this product as: Affinity Biosciences Cat# BF0255, RRID:AB_2833755.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

Purified recombinant fragment of human ABL2 expressed in E. Coli.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

ABL2

Molecular Weight:

Observed Mol.Wt.: 128kD.
Predicted Mol.Wt.: 128kDa(Calculated)..

Subcellular Location:

Cytoplasm, cytoskeleton.

Tissue Specificity:

P42684 ABL2_HUMAN:
Widely expressed.

Description:

ABL2 is a cytoplasmic tyrosine kinase which is closely related to but distinct from ABL1. The similarity of the proteins includes the tyrosine kinase domains and extends amino-terminal to include the SH2 and SH3 domains. ABL2 is expressed in both normal and tumor cells. The ABL2 gene product is expressed as two variants bearing different amino termini, both approximately 12-kb in length.

Sequence:
MGQQVGRVGEAPGLQQPQPRGIRGSSAARPSGRRRDPAGRTTETGFNIFTQHDHFASCVEDGFEGDKTGGSSPEALHRPYGCDVEPQALNEAIRWSSKENLLGATESDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNQNGEWSEVRSKNGQGWVPSNYITPVNSLEKHSWYHGPVSRSAAEYLLSSLINGSFLVRESESSPGQLSISLRYEGRVYHYRINTTADGKVYVTAESRFSTLAELVHHHSTVADGLVTTLHYPAPKCNKPTVYGVSPIHDKWEMERTDITMKHKLGGGQYGEVYVGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTLEPPFYIVTEYMPYGNLLDYLRECNREEVTAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHVVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNTFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYDLLEKGYRMEQPEGCPPKVYELMRACWKWSPADRPSFAETHQAFETMFHDSSISEEVAEELGRAASSSSVVPYLPRLPILPSKTRTLKKQVENKENIEGAQDATENSASSLAPGFIRGAQASSGSPALPRKQRDKSPSSLLEDAKETCFTRDRKGGFFSSFMKKRNAPTPPKRSSSFREMENQPHKKYELTGNFSSVASLQHADGFSFTPAQQEANLVPPKCYGGSFAQRNLCNDDGGGGGGSGTAGGGWSGITGFFTPRLIKKTLGLRAGKPTASDDTSKPFPRSNSTSSMSSGLPEQDRMAMTLPRNCQRSKLQLERTVSTSSQPEENVDRANDMLPKKSEESAAPSRERPKAKLLPRGATALPLRTPSGDLAITEKDPPGVGVAGVAAAPKGKEKNGGARLGMAGVPEDGEQPGWPSPAKAAPVLPTTHNHKVPVLISPTLKHTPADVQLIGTDSQGNKFKLLSEHQVTSSGDKDRPRRVKPKCAPPPPPVMRLLQHPSICSDPTEEPTALTAGQSTSETQEGGKKAALGAVPISGKAGRPVMPPPQVPLPTSSISPAKMANGTAGTKVALRKTKQAAEKISADKISKEALLECADLLSSALTEPVPNSQLVDTGHQLLDYCSGYVDCIPQTRNKFAFREAVSKLELSLQELQVSSAAAGVPGTNPVLNNLLSCVQEISDVVQR

Research Background

Function:

Non-receptor tyrosine-protein kinase that plays an ABL1-overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion and receptor endocytosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement); CTTN (involved in signaling); or TUBA1 and TUBB (microtubule subunits). Binds directly F-actin and regulates actin cytoskeletal structure through its F-actin-bundling activity. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL, DOK1 or ARHGAP35. Adhesion-dependent phosphorylation of ARHGAP35 promotes its association with RASA1, resulting in recruitment of ARHGAP35 to the cell periphery where it inhibits RHO. Phosphorylates multiple receptor tyrosine kinases like PDGFRB and other substrates which are involved in endocytosis regulation such as RIN1. In brain, may regulate neurotransmission by phosphorylating proteins at the synapse. ABL2 acts also as a regulator of multiple pathological signaling cascades during infection. Pathogens can highjack ABL2 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1.

Post-translational Modifications:

Phosphorylated at Tyr-261 by ABL1 in response to oxidative stress. Phosphorylated by PDGFRB (By similarity).

Polyubiquitinated. Polyubiquitination of ABL2 leads to degradation.

Subcellular Location:

Cytoplasm>Cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

Widely expressed.

Subunit Structure:

Interacts with PSMA7. Interacts with CTTN. Found in a complex with ABL1, ABL2, CRK and UNC119; leading to the inhibition of CRK phosphorylation by ABL kinases.

Similarity:

Contains two distinct classes of F-actin-binding domains. Although both can bind F-actin, the 2 are required to bundle actin filaments (By similarity).

Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.

Research Fields

Research Fields:

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway.(View pathway)
· Human Diseases > Cardiovascular diseases > Viral myocarditis.

No comment
Total 0 records, divided into1 pages. First Prev Next Last

Submit Review

Support JPG, GIF, PNG format only
captcha
Catalog Number :

BF0255-BP
(Blocking peptide available as BF0255-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

P42684 as Substrate
Site PTM Type Enzyme
R23 Methylation
S31 Phosphorylation
T68 Phosphorylation
S71 Phosphorylation
S72 Phosphorylation
S96 Phosphorylation
S97 Phosphorylation
Y116 Phosphorylation
S121 Phosphorylation
Y139 Phosphorylation P09619 (PDGFRB)
S159 Phosphorylation
Y161 Phosphorylation P09619 (PDGFRB)
T163 Phosphorylation
Y174 Phosphorylation
S194 Phosphorylation
S200 Phosphorylation
S203 Phosphorylation
S208 Phosphorylation
Y213 Phosphorylation
Y218 Phosphorylation
Y231 Phosphorylation
Y261 Phosphorylation P00519 (ABL1) , P42684 (ABL2)
Y272 Phosphorylation P42684 (ABL2) , P09619 (PDGFRB)
S275 Phosphorylation
Y299 Phosphorylation
Y303 Phosphorylation
Y310 Phosphorylation
S311 Phosphorylation
T313 Phosphorylation
K320 Acetylation
K331 Ubiquitination
T435 Phosphorylation
T438 Phosphorylation
Y439 Phosphorylation P42684 (ABL2)
T440 Phosphorylation
K446 Ubiquitination
T452 Phosphorylation
Y459 Phosphorylation
Y515 Phosphorylation
Y568 Phosphorylation P42684 (ABL2)
S577 Phosphorylation
K578 Ubiquitination
T581 Phosphorylation
S602 Phosphorylation
S617 Phosphorylation
S618 Phosphorylation
S620 Phosphorylation
S631 Phosphorylation
S633 Phosphorylation
S634 Phosphorylation
S655 Phosphorylation
K659 Acetylation
T664 Phosphorylation
K667 Methylation
S671 Phosphorylation
Y683 Phosphorylation P42684 (ABL2)
Y718 Phosphorylation
T753 Phosphorylation
S781 Phosphorylation
S783 Phosphorylation
T784 Phosphorylation
K809 Ubiquitination
T815 Phosphorylation
S817 Phosphorylation
T818 Phosphorylation
S819 Phosphorylation
S820 Phosphorylation
T864 Phosphorylation
S866 Phosphorylation
S915 Phosphorylation
S936 Phosphorylation
T938 Phosphorylation
K957 Methylation
K957 Ubiquitination
K959 Methylation
S969 Phosphorylation
K981 Acetylation
T1050 Phosphorylation
S1051 Phosphorylation
S1054 Phosphorylation
K1057 Methylation
K1066 Methylation
S1107 Phosphorylation
T1112 Phosphorylation
P42684 as PTM Enzyme
Substrate Site Source
O00401 (WASL) Y256 Uniprot
O14818 (PSMA7) Y153 Uniprot
O15304 (SIVA1) Y34 Uniprot
P01112 (HRAS) Y137 Uniprot
P04040 (CAT) Y231 Uniprot
P04040 (CAT) Y386 Uniprot
P05556 (ITGB1) Y783 Uniprot
P07203-1 (GPX1) Y98 Uniprot
P09619 (PDGFRB) Y686 Uniprot
P10636-8 (MAPT) Y197 Uniprot
P10636-8 (MAPT) Y310 Uniprot
P10636-8 (MAPT) Y394 Uniprot
P17676 (CEBPB) Y78 Uniprot
P17931 (LGALS3) Y79 Uniprot
P17931 (LGALS3) Y118 Uniprot
P42684 (ABL2) Y261 Uniprot
P42684 (ABL2) Y272 Uniprot
P42684 (ABL2) Y439 Uniprot
P42684 (ABL2) Y568 Uniprot
P42684 (ABL2) Y683 Uniprot
P46108 (CRK) Y221 Uniprot
Q06124 (PTPN11) Y584 Uniprot
Q13671 (RIN1) Y36 Uniprot
Q14247 (CTTN) Y421 Uniprot
Q14247 (CTTN) Y470 Uniprot
Q14247 (CTTN) Y486 Uniprot
Q9NRY4 (ARHGAP35) Y1105 Uniprot
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

To Top