Catenin gamma Antibody - #AF0124

(1)  
Product: Catenin gamma Antibody
Catalog: AF0124
Description: Rabbit polyclonal antibody to Catenin gamma
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 82kDa; 82kD(Calculated).
Uniprot: P14923
RRID: AB_2833308

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%), Bovine(88%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Catenin gamma Antibody detects endogenous levels of total Catenin gamma.
RRID:
AB_2833308
Cite Format: Affinity Biosciences Cat# AF0124, RRID:AB_2833308.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ARVD 12; ARVD12; Catenin (cadherin associated protein), gamma 80kDa; catenin (cadherin-associated protein) gamma (80kD); Catenin gamma; CTNNG; Desmoplakin 3; Desmoplakin III; Desmoplakin-3; Desmoplakin3; DesmoplakinIII; DP 3; DP III; DP3; DPIII; gamma catenin; Junction plakoglobin; JUP; OTTHUMP00000164732; OTTHUMP00000164735; OTTHUMP00000164738; PDGB; PKGB; PLAK_HUMAN; PLAKOGLOBIN;

Immunogens

Immunogen:
Uniprot:

P14923(PLAK_HUMAN) >>Visit HPA database.

Gene(ID):
JUP(3728)
Description:
desmoplakin 3 Common junctional plaque protein. The membrane- associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE- cadherin function in endothelial cells. Belongs to the beta-catenin family. Homodimer. Interacts with MUC1.
Sequence:
MEVMNLMEQPIKVTEWQQTYTYDSGIHSGANTCVPSVSSKGIMEEDEACGRQYTLKKTTTYTQGVPPSQGDLEYQMSTTARAKRVREAMCPGVSGEDSSLLLATQVEGQATNLQRLAEPSQLLKSAIVHLINYQDDAELATRALPELTKLLNDEDPVVVTKAAMIVNQLSKKEASRRALMGSPQLVAAVVRTMQNTSDLDTARCTTSILHNLSHHREGLLAIFKSGGIPALVRMLSSPVESVLFYAITTLHNLLLYQEGAKMAVRLADGLQKMVPLLNKNNPKFLAITTDCLQLLAYGNQESKLIILANGGPQALVQIMRNYSYEKLLWTTSRVLKVLSVCPSNKPAIVEAGGMQALGKHLTSNSPRLVQNCLWTLRNLSDVATKQEGLESVLKILVNQLSVDDVNVLTCATGTLSNLTCNNSKNKTLVTQNSGVEALIHAILRAGDKDDITEPAVCALRHLTSRHPEAEMAQNSVRLNYGIPAIVKLLNQPNQWPLVKATIGLIRNLALCPANHAPLQEAAVIPRLVQLLVKAHQDAQRHVAAGTQQPYTDGVRMEEIVEGCTGALHILARDPMNRMEIFRLNTIPLFVQLLYSSVENIQRVAAGVLCELAQDKEAADAIDAEGASAPLMELLHSRNEGTATYAAAVLFRISEDKNPDYRKRVSVELTNSLFKHDPAAWEAAQSMIPINEPYGDDMDATYRPMYSSDVPLDPLEMHMDMDGDYPIDTYSDGLRPPYPTADHMLA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Sheep
100
Dog
100
Rabbit
100
Pig
88
Bovine
88
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P14923 As Substrate

Site PTM Type Enzyme
M1 Acetylation
T14 O-Glycosylation
T19 Phosphorylation
Y20 Phosphorylation
T21 Phosphorylation
Y22 Phosphorylation
S24 Phosphorylation
K40 Ubiquitination
Y53 Phosphorylation
T54 Phosphorylation
K56 Ubiquitination
K57 Ubiquitination
T58 Phosphorylation
T59 Phosphorylation
T60 Phosphorylation
Y61 Phosphorylation
T62 Phosphorylation
S68 Phosphorylation
Y74 Phosphorylation
S77 Phosphorylation
S94 Phosphorylation
S99 Phosphorylation
T104 Phosphorylation
S120 Phosphorylation
K124 Ubiquitination
S125 Phosphorylation
Y133 Phosphorylation P06241 (FYN)
T141 Phosphorylation
K149 Ubiquitination
T160 Phosphorylation
K161 Ubiquitination
S170 Phosphorylation
K171 Ubiquitination
S182 Phosphorylation
T196 Phosphorylation
S197 Phosphorylation
K224 Ubiquitination
K272 Ubiquitination
K279 Ubiquitination
K326 Acetylation
K336 Ubiquitination
K345 Ubiquitination
K359 Ubiquitination
K385 Ubiquitination
K424 Ubiquitination
K426 Ubiquitination
T430 Phosphorylation
K448 Ubiquitination
T463 Phosphorylation
Y480 Phosphorylation
K499 Ubiquitination
T546 Phosphorylation
Y550 Phosphorylation P06241 (FYN) , P16591 (FER)
T551 Phosphorylation
T564 Phosphorylation
S596 Phosphorylation
Y644 Phosphorylation P12931 (SRC)
Y660 Phosphorylation
S665 Phosphorylation
T669 Phosphorylation
S671 Phosphorylation
Y701 Phosphorylation
Y724 Phosphorylation
T728 Phosphorylation
Y729 Phosphorylation
S730 Phosphorylation

Research Backgrounds

Function:

Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity).

PTMs:

May be phosphorylated by FER.

Subcellular Location:

Cell junction>Adherens junction. Cell junction>Desmosome. Cytoplasm>Cytoskeleton. Membrane>Peripheral membrane protein.
Note: Cytoplasmic in a soluble and membrane-associated form.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer. Component of an E-cadherin/catenin adhesion complex composed of at least E-cadherin/CDH1 and gamma-catenin/JUP, and possibly alpha-catenin/CTNNA1; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Interacts with MUC1. Interacts with CAV1 (By similarity). Interacts with PTPRJ. Interacts with DSG1. Interacts with DSC1 and DSC2. Interacts with PKP2.

Family&Domains:

The entire ARM repeats region mediates binding to CDH1/E-cadherin. The N-terminus and first three ARM repeats are sufficient for binding to DSG1. The N-terminus and first ARM repeat are sufficient for association with CTNNA1. DSC1 association requires both ends of the ARM repeat region.

Belongs to the beta-catenin family.

Research Fields

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

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