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$280 100ul
$350 200ul

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  • Product Name
    IL6 Antibody
  • Catalog No.
    DF6087
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC,ELISA
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Bovine(100%)
  • UniProt
  • Mol.Wt.
    24kDa
  • Concentration
    1mg/ml
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Product Information

Alternative Names:Expand▼

Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; B-cell stimulatory factor 2; BSF 2; BSF-2; BSF2; CDF; CTL differentiation factor; Hepatocyte stimulatory factor; HGF; HSF; Hybridoma growth factor; Hybridoma growth factor Interferon beta-2; IFN-beta-2; IFNB2; IL 6; IL-6; IL6; IL6_HUMAN; Interferon beta 2; Interferon beta-2; Interleukin 6; Interleukin 6 (interferon beta 2); Interleukin BSF 2; Interleukin-6;

Applications:

WB 1:500-1:2000, IHC 1:200, IF/ICC 1:200, ELISA(peptide) 1:20000-1:40000

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Bovine(100%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

IL6 Antibody detects endogenous levels of total IL6.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human IL6, corresponding to a region within the internal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

P05231

Molecular Weight:

Observed Mol.Wt.: 24kDa.
Predicted Mol.Wt.: 24kDa.

Subcellular Location:

Secreted.

Description:

Interleukin-6 (IL-6) is a multifunctional cytokine with a wide variety of biological functions. IL-6 is implicated in the final differentiation of B-cells into immunoglobulin-secreting cells (1), myeloma and plasmacytoma growth (2), nerve cell differentiation, and activation of hepatocytes and mitogen-stimulated helper T cells (3). Upon activation, IL-6 induces at least three major signaling pathways: JAK/STAT, PI-3 kinase and MAPK (4,5).

Sequence:
        10         20         30         40         50
MNSFSTSAFG PVAFSLGLLL VLPAAFPAPV PPGEDSKDVA APHRQPLTSS
60 70 80 90 100
ERIDKQIRYI LDGISALRKE TCNKSNMCES SKEALAENNL NLPKMAEKDG
110 120 130 140 150
CFQSGFNEET CLVKIITGLL EFEVYLEYLQ NRFESSEEQA RAVQMSTKVL
160 170 180 190 200
IQFLQKKAKN LDAITTPDPT TNASLLTKLQ AQNQWLQDMT THLILRSFKE
210
FLQSSLRALR QM

Background

Function:

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation.

Post-translational Modifications:

N- and O-glycosylated.

Subcellular Location:

Extracellular region or secreted;

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Similarity:

Belongs to the IL-6 superfamily.

Research Fields

Research Fields:

· Cellular Processes > Cell growth and death > Cellular senescence.(View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > FoxO signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.(View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.(View pathway)
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.(View pathway)
· Human Diseases > Immune diseases > Graft-versus-host disease.
· Human Diseases > Cancers: Overview > Pathways in cancer.(View pathway)
· Human Diseases > Immune diseases > Rheumatoid arthritis.
· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).
· Human Diseases > Infectious diseases: Bacterial > Legionellosis.
· Human Diseases > Infectious diseases: Bacterial > Pertussis.
· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.
· Human Diseases > Infectious diseases: Viral > Measles.
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).
· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.
· Human Diseases > Infectious diseases: Parasitic > African trypanosomiasis.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).
· Human Diseases > Infectious diseases: Parasitic > Malaria.
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
· Human Diseases > Neurodegenerative diseases > Prion diseases.
· Organismal Systems > Immune system > Hematopoietic cell lineage.(View pathway)
· Organismal Systems > Immune system > Toll-like receptor signaling pathway.(View pathway)
· Organismal Systems > Immune system > IL-17 signaling pathway.(View pathway)
· Organismal Systems > Immune system > Intestinal immune network for IgA production.(View pathway)
· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.(View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation.(View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway.(View pathway)

Reference Citations:

1). Zhao YZ et al. Ulcerative colitis-specific delivery of keratinocyte growth factor by neutrophils-simulated liposomes facilitates the morphologic and functional recovery of the damaged colon through alleviating the inflammation. J Control Release 2019 Feb 23 (PubMed: 30807805) [IF=7.901]

2). Qiu LL et al. Dysregulation of BDNF/TrkB signaling mediated by NMDAR/Ca2+/calpain might contribute to postoperative cognitive dysfunction in aging mice. J Neuroinflammation 2020 Jan 16;17(1):23 (PubMed: 31948437) [IF=5.700]

3). Liu W et al. Micheliolide Ameliorates Diabetic Kidney Disease by Inhibiting Mtdh-mediated Renal Inflammation in Type 2 Diabetic db/db Mice. Pharmacol Res 2019 Oct 24:104506 (PubMed: 31669149) [IF=5.574]

4). Zhang Y et al. Vagus Nerve Stimulation Attenuates Acute Skeletal Muscle Injury Induced by Ischemia-Reperfusion in Rats. Oxid Med Cell Longev 2019 Feb 28;2019:9208949 (PubMed: 30944700) [IF=4.868]

5). Chen C et al. A multifunctional-targeted nanoagent for dual-mode image-guided therapeutic effects on ovarian cancer cells. Int J Nanomedicine 2019 Jan 21;14:753-769 (PubMed: 30718954) [IF=4.471]

6). Han X et al. Autolysosomal degradation of cytosolic chromatin fragments antagonizes oxidative stress-induced senescence. J Biol Chem 2020 Feb 11 (PubMed: 32047109) [IF=4.167]

7). Yi L et al. Inflammation-mediated SOD-2 upregulation contributes to epithelial-mesenchymal transition and migration of tumor cells in aflatoxin G1-induced lung adenocarcinoma. Sci Rep 2017 Aug 11;7(1):7953 (PubMed: 28801561) [IF=4.011]

Application: WB    Species:human;    Sample:Not available

Figure 3. Tumor-associated infammatory microenvironment is elicited in AFG1-induced lung adenocarcinoma. Te RNA and protein samples as well as parafn sections isolated from the lungadenocarcinoma or control lung tissues were collected from our previous study. (a) Several cytokines and chemokines expression at mRNA level were determined by real-time PCR. Data was shown as mean±SD (n=4, *p<0.05, compared to control group). (b) Te expression of IL-6 and TNF-α at protein level was shown by Western blot. Band intensity is coming from densitometry, and data was shown as mean±SD (n=3, *p<0.05, compared to control group). (c) Representative immunohistochemical staining of IL-6 and TNF-α in AFG1-induced lung adenocarcinoma. (d) Representative immunohistochemical staining of CD68 in lung tissues close to lung adenocarcinoma and control lung tissues. Black arrow points to positive cell.

Application: IHC    Species:human;    Sample:Not available

Figure 3. Tumor-associated infammatory microenvironment is elicited in AFG1-induced lung adenocarcinoma. Te RNA and protein samples as well as parafn sections isolated from the lungadenocarcinoma or control lung tissues were collected from our previous study. (a) Several cytokines and chemokines expression at mRNA level were determined by real-time PCR. Data was shown as mean±SD (n=4, *p<0.05, compared to control group). (b) Te expression of IL-6 and TNF-α at protein level was shown by Western blot. Band intensity is coming from densitometry, and data was shown as mean±SD (n=3, *p<0.05, compared to control group). (c) Representative immunohistochemical staining of IL-6 and TNF-α in AFG1-induced lung adenocarcinoma. (d) Representative immunohistochemical staining of CD68 in lung tissues close to lung adenocarcinoma and control lung tissues. Black arrow points to positive cell.


8). Huang X et al. Leucovorin ameliorated methotrexate induced intestinal toxicity via modulation of the gut microbiota. Toxicol Appl Pharmacol 2020 Feb 19;391:114900 (PubMed: 32061593) [IF=3.564]

9). Lu L et al. Shexiang Baoxin Pill Alleviates the Atherosclerotic Lesions in Mice via Improving Inflammation Response and Inhibiting Lipid Accumulation in the Arterial Wall. Mediators Inflamm 2019 Jul 11;2019:6710759 (PubMed: 31379468) [IF=3.545]

10). Wang M et al. Berberine combined with cyclosporine A alleviates acute graft-versus-host disease in murine models. Int Immunopharmacol 2020 Feb 9;81:106205 (PubMed: 32050154) [IF=3.361]

11). Ji S et al. Resveratrol promotes oxidative stress to drive DLC1 mediated cellular senescence in cancer cells. Exp Cell Res 2018 Sep 15;370(2):292-302 (PubMed: 29964052) [IF=3.329]

12). Hu R et al. Salidroside ameliorates endothelial inflammation and oxidative stress by regulating the AMPK/NF-κB/NLRP3 signaling pathway in AGEs-induced HUVECs. Eur J Pharmacol 2020 Jan 15;867:172797 (PubMed: 31747547) [IF=3.170]

13). Huang G et al. Protective effects of icariin on dorsal random skin flap survival: An experimental study. Eur J Pharmacol 2019 Aug 8:172600 (PubMed: 31401155) [IF=3.170]

14). Li D et al. LPS-induced inflammation delays the transportation of ASP+ due to down-regulation of OCTN1/2 in alveolar epithelial cells. J Drug Target 2019 Oct 8:1-31 (PubMed: 31591905)

15). Feng X et al. Effects of Asiaticoside Treatment on the Survival of Random Skin Flaps in Rats. J Invest Surg 2019 Mar 21:1-11 (PubMed: 30898065)

16). Ren QC et al. Total flavonoids from sea buckthorn ameliorates lipopolysaccharide/cigarette smoke-induced airway inflammation. Phytother Res 2019 Jun 17 (PubMed: 31209984)

17). Chen LJ et al. Single xenotransplant of rat brown adipose tissue prolonged the ovarian lifespan of aging mice by improving follicle survival. Aging Cell 2019 Aug 6:e13024 (PubMed: 31389140)

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Catalog Number :

DF6087-BP
(Blocking peptide available as DF6087-BP)

Price/Size :

$200/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Bovine
100%
Dog
78%
Horse
71%
Pig
71%
Sheep
71%
Rabbit
0%
Chicken
0%
Xenopus
0%
Zebrafish
0%
High similarity Medium similarity Low similarity No similarity
P05231 as Substrate
Site PTM Type Enzyme
N73 N-Glycosylation
S81 Phosphorylation
K94 Ubiquitination
S135 Phosphorylation
S136 Phosphorylation
T165 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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