CRP Antibody - #DF6027

(1)   (4)  
Product: CRP Antibody
Catalog: DF6027
Description: Rabbit polyclonal antibody to CRP
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Rabbit
Mol.Wt.: 25kDa; 25kD(Calculated).
Uniprot: P02741
RRID: AB_2838002

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Rabbit(89%)
Clonality:
Polyclonal
Specificity:
CRP Antibody detects endogenous levels of total CRP.
RRID:
AB_2838002
Cite Format: Affinity Biosciences Cat# DF6027, RRID:AB_2838002.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Pentraxin 1, short; C reactive protein; C reactive protein pentraxin related; C-reactive protein(1-205); CRP; CRP_HUMAN; MGC88244; Pentraxin 1; PTX 1; PTX1;

Immunogens

Immunogen:
Uniprot:

P02741(CRP_HUMAN) >>Visit HPA database.

Gene(ID):
CRP(1401)
Expression:
P02741 CRP_HUMAN:

Found in plasma.

Description:
Human c-Reactive protein (CRP) was discovered in serum of patients as a nonantibody precipitin of the C-polysaccharide from pneumococci(1). During acute infection, the amounts of circulating CPR increases 100-1000 fold (2). Serum CRP values are routinely measured to detect or monitor many human diseases and minor elevations of C-reactive protein are predictive of cardiovascular events in patients with coronary heart disease (3). CRP promotes host defense, is an efficient activator of the classical C cascade, and shown to be opsonic (4).The opsonic and C activation activities are responsible for the nonspecific host defense activities providing protection in the preimmune stage of acute infections. Additionally, IL-1 and IL-6 are known to stimulate CRP biosynthesis (5).
Sequence:
MEKLLCFLVLTSLSHAFGQTDMSRKAFVFPKESDTSYVSLKAPLTKPLKAFTVCLHFYTELSSTRGYSIFSYATKRQDNEILIFWSKDIGYSFTVGGSEILFEVPEVTVAPVHICTSWESASGIVEFWVDGKPRVRKSLKKGYTVGAEASIILGQEQDSFGGNFEGSQSLVGDIGNVNMWDFVLSPDEINTIYLGGPFSPNVLNWRALKYEVQGEVFTKPQLWP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
89
Pig
0
Horse
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P02741 As Substrate

Site PTM Type Enzyme
K3 Methylation

Research Backgrounds

Function:

Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Found in plasma.

Subunit Structure:

Homopentamer. Pentraxin (or pentaxin) have a discoid arrangement of 5 non-covalently bound subunits. Interacts with FCN1; may regulate monocyte activation by FCN1.

Family&Domains:

Belongs to the pentraxin family.

References

1). Low-Frequency Vibration Promotes Tumor Necrosis Factor-α Production to Increase Cartilage Degeneration in Knee Osteoarthritis. Cartilage, 2021 (PubMed: 32532183) [IF=2.7]

Application: WB    Species: mouse    Sample: cartilage

Figure 3.| Expression of FAH, TIM2, and CRP in the cartilage in 4 groups. Expression of FAH, TIM2, and CRP in the cartilage was detected in different groups. FAH = fumarylacetoacetase hydrolase; TIM2 = tissue inhibitor of metalloproteinases 2; CRP =C-reactive protein; WBV = whole-body vibration; TNF-α = tumor necrosis factor-α; DMM = destabilization medial meniscus.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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