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$280 100ul
$350 200ul

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  • Product Name
    ATF6 Antibody
  • Catalog No.
    DF6009
  • RRID
    AB_2833019
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC,ELISA
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Pig(100%), Zebrafish(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
  • UniProt
  • Mol.Wt
    (Observed)75kD; (Calculated)75kDa
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

Activating transcription factor 6 alpha; Activating transcription factor 6; ATF 6; ATF6 alpha; ATF6; ATF6-alpha; ATF6A; ATF6A_HUMAN; cAMP dependent transcription factor ATF 6 alpha; cAMP-dependent transcription factor ATF-6 alpha; Cyclic AMP dependent transcription factor ATF 6 alpha; DKFZp686P2194; ESTM49; FLJ21663; Processed cyclic AMP dependent transcription factor ATF 6 alpha; Processed cyclic AMP-dependent transcription factor ATF-6 alpha;

Applications:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Pig(100%), Zebrafish(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

ATF6 Antibody detects endogenous levels of total ATF6.

RRID:

AB_2833019
Please cite this product as: Affinity Biosciences Cat# DF6009, RRID:AB_2833019.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human ATF6, corresponding to a region within the internal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

ATF6

Molecular Weight:

Observed Mol.Wt.: (Observed)75kD.
Predicted Mol.Wt.: (Calculated)75kDa.

Subcellular Location:

Endoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.

Tissue Specificity:

P18850 ATF6A_HUMAN:
Ubiquitous.

Description:

This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis. [provided by RefSeq, Aug 2011]

Sequence:
MGEPAGVAGTMESPFSPGLFHRLDEDWDSALFAELGYFTDTDELQLEAANETYENNFDNLDFDLDLMPWESDIWDINNQICTVKDIKAEPQPLSPASSSYSVSSPRSVDSYSSTQHVPEELDLSSSSQMSPLSLYGENSNSLSSAEPLKEDKPVTGPRNKTENGLTPKKKIQVNSKPSIQPKPLLLPAAPKTQTNSSVPAKTIIIQTVPTLMPLAKQQPIISLQPAPTKGQTVLLSQPTVVQLQAPGVLPSAQPVLAVAGGVTQLPNHVVNVVPAPSANSPVNGKLSVTKPVLQSTMRNVGSDIAVLRRQQRMIKNRESACQSRKKKKEYMLGLEARLKAALSENEQLKKENGTLKRQLDEVVSENQRLKVPSPKRRVVCVMIVLAFIILNYGPMSMLEQDSRRMNPSVSPANQRRHLLGFSAKEAQDTSDGIIQKNSYRYDHSVSNDKALMVLTEEPLLYIPPPPCQPLINTTESLRLNHELRGWVHRHEVERTKSRRMTNNQQKTRILQGALEQGSNSQLMAVQYTETTSSISRNSGSELQVYYASPRSYQDFFEAIRRRGDTFYVVSFRRDHLLLPATTHNKTTRPKMSIVLPAININENVINGQDYEVMMQIDCQVMDTRILHIKSSSVPPYLRDQQRNQTNTFFGSPPAATEATHVVSTIPESLQ

Research Background

Function:

Transmembrane glycoprotein of the endoplasmic reticulum that functions as a transcription activator and initiates the unfolded protein response (UPR) during endoplasmic reticulum stress. Cleaved upon ER stress, the N-terminal processed cyclic AMP-dependent transcription factor ATF-6 alpha translocates to the nucleus where it activates transcription of genes involved in the UPR. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. May play a role in foveal development and cone function in the retina.

Post-translational Modifications:

During unfolded protein response, a fragment of approximately 50 kDa containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases.

N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR).

Phosphorylated in vitro by MAPK14/P38MAPK.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass type II membrane protein.

Nucleus.
Note: Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. THBS4 promotes its nuclear shuttling.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Homodimer and heterodimer with ATF6-beta. The dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC). Interacts also with the transcription factors GTF2I, YY1 and SRF. Interacts (via lumenal domain) with THBS1 (By similarity). Interacts with THBS4 (via EGF-like 3; calcium-binding domain) which facilitates its processing, activation and nuclear translocation (PubMed:22682248). Interacts with XBP1 isoform 2; the interaction occurs in a ER stress-dependent manner (PubMed:17765680).

Similarity:

The basic domain functions as a nuclear localization signal.

The basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE.

Belongs to the bZIP family. ATF subfamily.

Research Fields

Research Fields:

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.(View pathway)
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

Reference Citations:

1). Li Y et al. Branched chain amino acids exacerbate myocardial ischemia/reperfusion vulnerability via enhancing GCN2/ATF6/PPAR-α pathway-dependent fatty acid oxidation. Theranostics 2020 Apr 27;10(12):5623-5640 (PubMed: 32373236) [IF=8.063]

2). Wu X et al. Protective Effect of Patchouli Alcohol Against High-Fat Diet Induced Hepatic Steatosis by Alleviating Endoplasmic Reticulum Stress and Regulating VLDL Metabolism in Rats. Front Pharmacol 2019 Oct 1;10:1134 (PubMed: 31632274) [IF=3.845]

3). Xu A;Shang W;Wang Y;Sun X;Zhou B;Xie Y;Xu X;Liu T;Han F; et al. ALA Protects Against ERS-mediated Apoptosis in a Cochlear Cell Model With Low Citrate Synthase Expression. Arch Biochem Biophys 2020 May 11;688:108402. (PubMed: 32418909) [IF=3.559]

4). Yang M et al. Therapeutic effects of scavenger receptor MARCO ligand on silica-induced pulmonary fibrosis in rats. Toxicol Lett 2019 Apr 24;311:1-10 (PubMed: 31028789) [IF=3.499]

5). Chen X et al. Oleic acid protects saturated fatty acid mediated lipotoxicity in hepatocytes and rat of non-alcoholic steatohepatitis. Life Sci 2018 Jun 15;203:291-304 (PubMed: 29709653) [IF=3.448]

6). Su R et al. The potential immunotoxicity of fine particulate matter based on SD rat spleen. Environ Sci Pollut Res Int 2019 Aug;26(23):23958-23966 (PubMed: 31218585)

7). Qiuli H et al. [EXPRESS] Endoplasmic reticulum stress promoting caspase signaling pathway dependent apoptosis contributes to bone cancer pain in the spinal dorsal horn. Mol Pain 2019 Aug 27:1744806919876150 (PubMed: 31452457)

8). Sun X et al. Hepatic conditional knockout of ATF6 exacerbates liver metabolic damage by repressing autophage through MTOR pathway. Biochem Biophys Res Commun 2018 Oct 20;505(1):45-50 (PubMed: 30236984)

9). Lu HY et al. GRP78 silencing enhances hyperoxia-induced alveolar epithelial cell apoptosis via CHOP pathway. Mol Med Rep 2017 Aug;16(2):1493-1501 (PubMed: 28586043)

10). Yang X et al. SWNHs (Single-Wall Carbon Nanohorns) Supervises Endoplasmic Reticulum (ER) Stress in Hepatocellular Carcinoma. J Nanosci Nanotechnol 2018 Oct 1;18(10):6740-6745 (PubMed: 29954489)

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Catalog Number :

DF6009-BP
(Blocking peptide available as DF6009-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Xenopus
100%
Zebrafish
100%
Rabbit
100%
Pig
100%
Dog
100%
Sheep
100%
Horse
100%
Bovine
0%
Chicken
0%
High similarity Medium similarity Low similarity No similarity
P18850 as Substrate
Site PTM Type Enzyme
S16 Phosphorylation
K87 Sumoylation
K87 Ubiquitination
S94 Phosphorylation
Y100 Phosphorylation
S104 Phosphorylation
K152 Sumoylation
T166 Phosphorylation Q16539 (MAPK14)
K176 Acetylation
K176 Sumoylation
K176 Ubiquitination
K182 Sumoylation
K191 Sumoylation
K201 Ubiquitination
K216 Ubiquitination
K290 Sumoylation
K290 Ubiquitination
T296 Phosphorylation
Y330 Phosphorylation
K339 Ubiquitination
K349 Ubiquitination
S373 Phosphorylation
S410 Phosphorylation
K424 Ubiquitination
K436 Ubiquitination
N472 N-Glycosylation
K506 Ubiquitination
T565 Phosphorylation
N584 N-Glycosylation
K629 Ubiquitination
S632 Phosphorylation
N643 N-Glycosylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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