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  • Product Name
    PDGFRbeta Antibody
  • Catalog No.
    BF0289
  • RRID
    AB_2833717
  • Source
    Mouse
  • Application
    WB,ELISA
  • Reactivity
    Human, Mouse
  • UniProt
  • Mol.Wt
    190kD;
    124kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

Product Information

Alternative Names:Expand▼

Beta platelet derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD 140B; CD140 antigen-like family member B; CD140b; CD140b antigen; IBGC4; IMF1; JTK12; OTTHUMP00000160528; PDGF R beta; PDGF-R-beta; PDGFR 1; PDGFR; PDGFR beta; PDGFR1; PDGFRB; PGFRB_HUMAN; Platelet derived growth factor receptor 1; Platelet derived growth factor receptor beta; Platelet derived growth factor receptor beta polypeptide;

Applications:

ELISA 1:10000, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse

Source:

Mouse

Clonality:

Monoclonal

Clone Number:

AFB1693

Purification:

Affinity-chromatography.

Specificity:

PDGFRbeta antibody detects endogenous levels of total PDGFRbeta.

RRID:

AB_2833717
Please cite this product as: Affinity Biosciences Cat# BF0289, RRID:AB_2833717.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

Purified recombinant fragment of human PDGFRbeta expressed in E. Coli.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

PDGFRB

Molecular Weight:

Observed Mol.Wt.: 190kD.
Predicted Mol.Wt.: 124kDa(Calculated)..

Subcellular Location:

Membrane.

Description:

Platelet-derived growth factor receptor, beta polypeptide, also known as PDGFRB, PDGFRbeta or CD140B. It is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. This family exist as several disulphide-bonded, dimeric isoforms (PDGF AA, PDGF AB, PDGF BB, PDGF CC and PDGF DD) that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor α (PDGFRα) and PDGF receptor beta (PDGFRbeta). These growth factors are mitogens for cells of mesenchymal origin. PDGFRB is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.

Sequence:
MRLPGAMPALALKGELLLLSLLLLLEPQISQGLVVTPPGPELVLNVSSTFVLTCSGSAPVVWERMSQEPPQEMAKAQDGTFSSVLTLTNLTGLDTGEYFCTHNDSRGLETDERKRLYIFVPDPTVGFLPNDAEELFIFLTEITEITIPCRVTDPQLVVTLHEKKGDVALPVPYDHQRGFSGIFEDRSYICKTTIGDREVDSDAYYVYRLQVSSINVSVNAVQTVVRQGENITLMCIVIGNEVVNFEWTYPRKESGRLVEPVTDFLLDMPYHIRSILHIPSAELEDSGTYTCNVTESVNDHQDEKAINITVVESGYVRLLGEVGTLQFAELHRSRTLQVVFEAYPPPTVLWFKDNRTLGDSSAGEIALSTRNVSETRYVSELTLVRVKVAEAGHYTMRAFHEDAEVQLSFQLQINVPVRVLELSESHPDSGEQTVRCRGRGMPQPNIIWSACRDLKRCPRELPPTLLGNSSEEESQLETNVTYWEEEQEFEVVSTLRLQHVDRPLSVRCTLRNAVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQKKPRYEIRWKVIESVSSDGHEYIYVDPMQLPYDSTWELPRDQLVLGRTLGSGAFGQVVEATAHGLSHSQATMKVAVKMLKSTARSSEKQALMSELKIMSHLGPHLNVVNLLGACTKGGPIYIITEYCRYGDLVDYLHRNKHTFLQHHSDKRRPPSAELYSNALPVGLPLPSHVSLTGESDGGYMDMSKDESVDYVPMLDMKGDVKYADIESSNYMAPYDNYVPSAPERTCRATLINESPVLSYMDLVGFSYQVANGMEFLASKNCVHRDLAARNVLICEGKLVKICDFGLARDIMRDSNYISKGSTFLPLKWMAPESIFNSLYTTLSDVWSFGILLWEIFTLGGTPYPELPMNEQFYNAIKRGYRMAQPAHASDEIYEIMQKCWEEKFEIRPPFSQLVLLLERLLGEGYKKKYQQVDEEFLRSDHPAILRSQARLPGFHGLRSPLDTSSVLYTAVQPNEGDNDYIIPLPDPKPEVADEGPLEGSPSLASSTLNEVNTSSTISCDSPLEPQDEPEPEPQLELQVEPEPELEQLPDSGCPAPRAEAEDSFL

Research Background

Function:

Tyrosine-protein kinase that acts as cell-surface receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis and migration. Plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. Required for normal development of the cardiovascular system. Required for normal recruitment of pericytes (mesangial cells) in the kidney glomerulus, and for normal formation of a branched network of capillaries in kidney glomeruli. Promotes rearrangement of the actin cytoskeleton and the formation of membrane ruffles. Binding of its cognate ligands - homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFD -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PLCG1, PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1 and NCK1. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to the activation of the AKT1 signaling pathway. Phosphorylation of SHC1, or of the C-terminus of PTPN11, creates a binding site for GRB2, resulting in the activation of HRAS, RAF1 and down-stream MAP kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation and activation of SRC family kinases. Promotes phosphorylation of PDCD6IP/ALIX and STAM. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.

Post-translational Modifications:

Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-579, and to a lesser degree, at Tyr-581, is important for interaction with SRC family kinases. Phosphorylation at Tyr-740 and Tyr-751 is important for interaction with PIK3R1. Phosphorylation at Tyr-751 is important for interaction with NCK1. Phosphorylation at Tyr-771 and Tyr-857 is important for interaction with RASA1/GAP. Phosphorylation at Tyr-857 is important for efficient phosphorylation of PLCG1 and PTPN11, resulting in increased phosphorylation of AKT1, MAPK1/ERK2 and/or MAPK3/ERK1, PDCD6IP/ALIX and STAM, and in increased cell proliferation. Phosphorylation at Tyr-1009 is important for interaction with PTPN11. Phosphorylation at Tyr-1009 and Tyr-1021 is important for interaction with PLCG1. Phosphorylation at Tyr-1021 is important for interaction with CBL; PLCG1 and CBL compete for the same binding site. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021. Dephosphorylated by PTPN2 at Tyr-579 and Tyr-1021.

N-glycosylated.

Ubiquitinated. After autophosphorylation, the receptor is polyubiquitinated, leading to its degradation.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Cytoplasmic vesicle. Lysosome lumen.
Note: After ligand binding, the autophosphorylated receptor is ubiquitinated and internalized, leading to its degradation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

Interacts with homodimeric PDGFB and PDGFD, and with heterodimers formed by PDGFA and PDGFB. May also interact with homodimeric PDGFC. Monomer in the absence of bound ligand. Interaction with homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFD, leads to receptor dimerization, where both PDGFRA homodimers and heterodimers with PDGFRB are observed. Interacts with SH2B2/APS. Interacts directly (tyrosine phosphorylated) with SHB. Interacts (tyrosine phosphorylated) with PIK3R1 and RASA1. Interacts (tyrosine phosphorylated) with CBL. Interacts (tyrosine phosphorylated) with SRC and SRC family kinases. Interacts (tyrosine phosphorylated) with PIK3C2B, maybe indirectly. Interacts (tyrosine phosphorylated) with SHC1, GRB7, GRB10 and NCK1. Interaction with GRB2 is mediated by SHC1. Interacts (via C-terminus) with SLC9A3R1.

Similarity:

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

Research Fields

Research Fields:

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.(View pathway)
· Cellular Processes > Cellular community - eukaryotes > Gap junction.(View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.(View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Calcium signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway.(View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.(View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.(View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Glioma.(View pathway)
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.(View pathway)
· Human Diseases > Cancers: Overview > Choline metabolism in cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Melanoma.(View pathway)
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Human Diseases > Cancers: Specific types > Prostate cancer.(View pathway)

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Catalog Number :

BF0289-BP
(Blocking peptide available as BF0289-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

P09619 as Substrate
Site PTM Type Enzyme
N89 N-Glycosylation
N103 N-Glycosylation
S212 Phosphorylation
S213 Phosphorylation
N215 N-Glycosylation
N230 N-Glycosylation
N292 N-Glycosylation
N307 N-Glycosylation
Y562 Phosphorylation P09619 (PDGFRB)
Y579 Phosphorylation P09619 (PDGFRB)
Y581 Phosphorylation P09619 (PDGFRB)
T605 Phosphorylation
S608 Phosphorylation
K645 Ubiquitination
Y678 Phosphorylation
Y683 Phosphorylation
Y686 Phosphorylation P42684 (ABL2) , P00519 (ABL1)
Y692 Phosphorylation
S705 Phosphorylation
K707 Ubiquitination
S712 Phosphorylation
Y716 Phosphorylation P09619 (PDGFRB)
S717 Phosphorylation
Y740 Phosphorylation P09619 (PDGFRB)
Y751 Phosphorylation P09619 (PDGFRB)
Y763 Phosphorylation P09619 (PDGFRB)
Y771 Phosphorylation P09619 (PDGFRB)
Y775 Phosphorylation P09619 (PDGFRB)
Y778 Phosphorylation P09619 (PDGFRB)
Y857 Phosphorylation P09619 (PDGFRB)
Y921 Phosphorylation
S930 Phosphorylation
Y934 Phosphorylation P00519 (ABL1)
Y970 Phosphorylation P00519 (ABL1)
Y1009 Phosphorylation P09619 (PDGFRB)
Y1021 Phosphorylation P09619 (PDGFRB)
S1104 Phosphorylation
P09619 as PTM Enzyme
Substrate Site Source
P06241-3 (FYN) Y28 Uniprot
P09619 (PDGFRB) Y562 Uniprot
P09619 (PDGFRB) Y579 Uniprot
P09619 (PDGFRB) Y581 Uniprot
P09619-1 (PDGFRB) Y716 Uniprot
P09619 (PDGFRB) Y740 Uniprot
P09619 (PDGFRB) Y751 Uniprot
P09619-1 (PDGFRB) Y763 Uniprot
P09619-1 (PDGFRB) Y771 Uniprot
P09619 (PDGFRB) Y775 Uniprot
P09619 (PDGFRB) Y778 Uniprot
P09619-1 (PDGFRB) Y857 Uniprot
P09619 (PDGFRB) Y1009 Uniprot
P09619 (PDGFRB) Y1021 Uniprot
P12931 (SRC) Y139 Uniprot
P12931 (SRC) Y419 Uniprot
P15941 (MUC1) Y1203 Uniprot
P15941 (MUC1) Y1218 Uniprot
P16234 (PDGFRA) Y754 Uniprot
P27986 (PIK3R1) Y508 Uniprot
P34947 (GRK5) Y90 Uniprot
P34947 (GRK5) Y109 Uniprot
P34947 (GRK5) Y309 Uniprot
P34947 (GRK5) Y368 Uniprot
P42684 (ABL2) Y139 Uniprot
P42684 (ABL2) Y161 Uniprot
P42684 (ABL2) Y272 Uniprot
Q05397 (PTK2) Y5 Uniprot
Q05397 (PTK2) Y194 Uniprot
Q05655 (PRKCD) Y313 Uniprot
Q05655 (PRKCD) Y334 Uniprot
Q06124-2 (PTPN11) Y542 Uniprot
Q06124 (PTPN11) Y546 Uniprot
Q07912 (TNK2) Y635 Uniprot
Q13480 (GAB1) Y627 Uniprot
Q13480 (GAB1) Y659 Uniprot
Q13769 (THOC5) Y225 Uniprot
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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