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$150 50ul
$250 100ul
$350 200ul
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  • Product Name
    Lamin B Antibody
  • Catalog No.
    AF5161
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC,ELISA
  • Reactivity:
    Human, Mouse, Rat, Monkey, Fish
  • Prediction:
    Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Xenopus(83%)
  • UniProt
  • Mol.Wt.
    66 kDa
  • Concentration
    1mg/ml
  • Browse similar products>>

Product Information

Alternative Names:Expand▼

ADLD; lamin B1; Lamin-B1; LMN; LMN2; LMNB; Lmnb1; LMNB1_HUMAN; MGC111419; OTTHUMP00000159218;

Applications:

WB 1:500-1:2000, IHC 1:50-1:200, IF 1:200, ELISA(peptide) 1:20000-1:40000

Reactivity:

Human, Mouse, Rat, Monkey, Fish

Predicted Reactivity:

Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Xenopus(83%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

Lamin B Antibody detects endogenous levels of total Lamin B.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human Lamin B.

Uniprot:



>>Visit The Human Protein Atlas

Gene id:

Molecular Weight:

Observed Mol.Wt.: 66 kDa.
Predicted Mol.Wt.: 67kDa.

Subcellular Location:

Nucleus inner membrane.

Description:

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

Sequence:
        10         20         30         40         50
MATATPVPPR MGSRAGGPTT PLSPTRLSRL QEKEELRELN DRLAVYIDKV
60 70 80 90 100
RSLETENSAL QLQVTEREEV RGRELTGLKA LYETELADAR RALDDTARER
110 120 130 140 150
AKLQIELGKC KAEHDQLLLN YAKKESDLNG AQIKLREYEA ALNSKDAALA
160 170 180 190 200
TALGDKKSLE GDLEDLKDQI AQLEASLAAA KKQLADETLL KVDLENRCQS
210 220 230 240 250
LTEDLEFRKS MYEEEINETR RKHETRLVEV DSGRQIEYEY KLAQALHEMR
260 270 280 290 300
EQHDAQVRLY KEELEQTYHA KLENARLSSE MNTSTVNSAR EELMESRMRI
310 320 330 340 350
ESLSSQLSNL QKESRACLER IQELEDLLAK EKDNSRRMLT DKEREMAEIR
360 370 380 390 400
DQMQQQLNDY EQLLDVKLAL DMEISAYRKL LEGEEERLKL SPSPSSRVTV
410 420 430 440 450
SRASSSRSVR TTRGKRKRVD VEESEASSSV SISHSASATG NVCIEEIDVD
460 470 480 490 500
GKFIRLKNTS EQDQPMGGWE MIRKIGDTSV SYKYTSRYVL KAGQTVTIWA
510 520 530 540 550
ANAGVTASPP TDLIWKNQNS WGTGEDVKVI LKNSQGEEVA QRSTVFKTTI
560 570 580
PEEEEEEEEA AGVVVEEELF HQQGTPRASN RSCAIM

Background

Function:

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

Post-translational Modifications:

B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.

Subcellular Location:

Nucleus;

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

Homodimer. Interacts with lamin-associated polypeptides IA, IB and 2. Interacts with SPAG4 and SEPT12.

Similarity:

Belongs to the intermediate filament family.

Research Fields

Research Fields:

· Cellular Processes > Cell growth and death > Apoptosis.(View pathway)

Western blot analysis of extracts from Hela, using Lamin B Antibody. The lane on the left was treated with blocking peptide.
Western blot analysis of extracts from various samples, using Lamin B Antibody. Lane 1: Rat brain treated with blocking peptide; Lane 2: Rat brain; Lane 3: Myeloma cells; Lane 4: Rat heart; Lane 5: HUVEC.
AF5161 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF5161 at 1/100 staining human Pancreas cancer tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hou
AF5161 staining lovo cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondry antibody

Reference Citations:

1). Wang N et al. Fibroblast Growth Factor 21 Exerts Its Anti-inflammatory Effects on Multiple Cell Types of Adipose Tissue in Obesity. Obesity (Silver Spring) 2019 Jan 31 (PubMed: 30703283) [IF=3.969]

2). Tan F et al. Matrix stiffness mediates stemness characteristics via activating the Yes-associated protein in colorectal cancer cells. J Cell Biochem 2018 Sep 14 (PubMed: 30218452) [IF=3.448]

3). Tan F et al. Matrix stiffness mediates stemness characteristics via activating the Yes-associated protein in colorectal cancer cells. J Cell Biochem 2018 Sep 14 (PubMed: 30218452) [IF=3.448]

4). Wang N et al. Fibroblast growth factor 21 ameliorates pancreatic fibrogenesis via regulating polarization of macrophages. Exp Cell Res 2019 Jun 5 (PubMed: 31175853) [IF=3.329]

5). Zheng L et al. Selenium deficiency impaired immune function of the immune organs in young grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol 2018 Jun;77:53-70 (PubMed: 29559270) [IF=3.298]

6). Huang C et al. Deoxynivalenol decreased intestinal immune function related to NF-κB and TOR signalling in juvenile grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol 2018 Oct 16;84:470-484 (PubMed: 30339843) [IF=3.298]

7). Zheng L et al. Selenium deficiency impaired structural integrity of the head kidney, spleen and skin in young grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol 2018 Nov;82:408-420 (PubMed: 30142391) [IF=3.298]

8). Zhong JR et al. Phytic acid disrupted intestinal immune status and suppressed growth performance in on-growing grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol 2019 Jun 24;92:536-551 (PubMed: 31247320) [IF=3.298]

9). Wang KZ et al. Dietary gossypol reduced intestinal immunity and aggravated inflammation in on-growing grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol 2018 Dec 10 (PubMed: 30543935) [IF=3.298]

10). Peng XR et al. Supplementation exogenous bile acid improved growth and intestinal immune function associated with NF-κB and TOR signalling pathways in on-growing grass carp (Ctenopharyngodon idella): Enhancement the effect of protein-sparing by dietary lipid. Fish Shellfish Immunol 2019 Jun 26;92:552-569 (PubMed: 31252043) [IF=3.298]

11). Yu Y et al. Fibroblast growth factor (FGF21) protects mouse liver against D-galactose-induced oxidative stress and apoptosis via activating Nrf2 and PI3K/Akt pathways. Mol Cell Biochem 2015 May;403(1-2):287-99 (PubMed: 25701356)

Application: WB    Species:Not available;    Sample:Not available


12). Ding S et al. Combined effects of ambient particulate matter exposure and a high-fat diet on oxidative stress and steatohepatitis in mice. PLoS One 2019 Mar 28;14(3):e0214680 (PubMed: 30921449)

13). Yu Y et al. Fibroblast growth factor 21 protects mouse brain against D-galactose induced aging via suppression of oxidative stress response and advanced glycation end products formation. Pharmacol Biochem Behav 2015 Jun;133:122-31 (PubMed: 25871519)

Application: WB    Species:mouse;    Sample:mouse brain


14). Wu Q et al. The bispecific antibody aimed at the vicious circle of IL-1β and IL-17A, is beneficial for the collagen-induced rheumatoid arthritis of mice through NF-κB signaling pathway. Immunol Lett 2016 Nov;179:68-79 (PubMed: 27616043)

Application: WB    Species:mouse;    Sample:Not available


15). et al. Erucic acid inhibits growth performance and disrupts intestinal structural integrity of on-growing grass carp (Ctenopharyngodon idella).

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Catalog Number :

AF5161-BP
(Blocking peptide available as AF5161-BP)

Price/Size :

$200/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Bovine
100%
Sheep
100%
Rabbit
100%
Xenopus
83%
Chicken
70%
Zebrafish
58%
Dog
0%
Horse
0%
High similarity Medium similarity Low similarity No similarity
P20700 as Substrate
Site PTM Type Enzyme
A2 Acetylation
T3 Phosphorylation
T5 Phosphorylation
R10 Methylation
S13 Phosphorylation
R14 Methylation
T19 Phosphorylation
T20 Phosphorylation
S23 Phosphorylation P06493 (CDK1)
T25 Phosphorylation
S28 Phosphorylation
K33 Acetylation
K33 Ubiquitination
R42 Methylation
Y46 Phosphorylation
K49 Acetylation
K49 Ubiquitination
S58 Phosphorylation
K79 Acetylation
K79 Ubiquitination
Y82 Phosphorylation
T84 Phosphorylation
K102 Acetylation
K102 Sumoylation
K102 Ubiquitination
K109 Ubiquitination
K111 Acetylation
K111 Ubiquitination
K123 Acetylation
K123 Ubiquitination
K124 Ubiquitination
S126 Phosphorylation
K134 Acetylation
K134 Ubiquitination
Y138 Phosphorylation
S144 Phosphorylation
K145 Ubiquitination
K156 Acetylation
K156 Ubiquitination
K157 Acetylation
K157 Ubiquitination
S158 Phosphorylation
K167 Ubiquitination
K181 Acetylation
K181 Ubiquitination
K182 Ubiquitination
R197 Methylation
C198 S-Nitrosylation
S200 Phosphorylation
K209 Acetylation
K209 Ubiquitination
S210 Phosphorylation
Y212 Phosphorylation
K222 Ubiquitination
S232 Phosphorylation
Y238 Phosphorylation
K241 Sumoylation
K241 Ubiquitination
R250 Methylation
K261 Sumoylation
K261 Ubiquitination
T267 Phosphorylation
Y268 Phosphorylation
K271 Acetylation
K271 Ubiquitination
S278 Phosphorylation
S279 Phosphorylation
T283 Phosphorylation
S284 Phosphorylation
T285 Phosphorylation
S288 Phosphorylation
R297 Methylation
R299 Methylation
S302 Phosphorylation
S304 Phosphorylation
S305 Phosphorylation
K312 Acetylation
K312 Ubiquitination
K330 Ubiquitination
T340 Phosphorylation
K342 Ubiquitination
Y360 Phosphorylation
S375 Phosphorylation
Y377 Phosphorylation
K379 Acetylation
K379 Sumoylation
K379 Ubiquitination
R387 Methylation
K389 Sumoylation
K389 Ubiquitination
S391 Phosphorylation
S393 Phosphorylation P06493 (CDK1)
S395 Phosphorylation P05771 (PRKCB)
S396 Phosphorylation
T399 Phosphorylation
S401 Phosphorylation
S404 Phosphorylation
S405 Phosphorylation P05771 (PRKCB)
S406 Phosphorylation
S408 Phosphorylation
T411 Phosphorylation
T412 Phosphorylation
K457 Ubiquitination
S460 Phosphorylation
K474 Ubiquitination
S479 Phosphorylation
S481 Phosphorylation
K483 Acetylation
K483 Ubiquitination
K528 Ubiquitination
K532 Acetylation
K532 Ubiquitination
S534 Phosphorylation
K547 Ubiquitination
T575 Phosphorylation
S579 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.