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  • Product Name
    ZO 1 Antibody
  • Catalog No.
    AF5145
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC,ELISA
  • Reactivity
    Human, Mouse, Rat, Pig, Monkey
  • Prediction
    Horse(85%), Dog(92%)
  • UniProt
  • Mol.Wt.
    195 kDa,196kDa
  • Concentration
    1mg/ml
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Product Information

Alternative Names:Expand▼

Tight junction protein 1; Tight junction protein ZO-1; Tight junction protein ZO1; TJP1; zo-1; Zo1; ZO1_HUMAN; Zona occludens 1; Zona occludens 1 protein; Zona occludens protein 1; Zonula occludens 1 protein; Zonula occludens protein 1;

Applications:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Reactivity:

Human, Mouse, Rat, Pig, Monkey

Predicted Reactivity:

Horse(85%), Dog(92%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

ZO 1 Antibody detects endogenous levels of total ZO 1.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human ZO 1, corresponding to a region within the internal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

Q07157

Molecular Weight:

Observed Mol.Wt.: 195 kDa.
Predicted Mol.Wt.: 196kDa.

Subcellular Location:

Cell membrane. Cell junction > tight junction. Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact.

Tissue Specificity:

The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.

Description:

The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.

Sequence:
        10         20         30         40         50
MSARAAAAKS TAMEETAIWE QHTVTLHRAP GFGFGIAISG GRDNPHFQSG
60 70 80 90 100
ETSIVISDVL KGGPAEGQLQ ENDRVAMVNG VSMDNVEHAF AVQQLRKSGK
110 120 130 140 150
NAKITIRRKK KVQIPVSRPD PEPVSDNEED SYDEEIHDPR SGRSGVVNRR
160 170 180 190 200
SEKIWPRDRS ASRERSLSPR SDRRSVASSQ PAKPTKVTLV KSRKNEEYGL
210 220 230 240 250
RLASHIFVKE ISQDSLAARD GNIQEGDVVL KINGTVTENM SLTDAKTLIE
260 270 280 290 300
RSKGKLKMVV QRDERATLLN VPDLSDSIHS ANASERDDIS EIQSLASDHS
310 320 330 340 350
GRSHDRPPRR SRSRSPDQRS EPSDHSRHSP QQPSNGSLRS RDEERISKPG
360 370 380 390 400
AVSTPVKHAD DHTPKTVEEV TVERNEKQTP SLPEPKPVYA QVGQPDVDLP
410 420 430 440 450
VSPSDGVLPN STHEDGILRP SMKLVKFRKG DSVGLRLAGG NDVGIFVAGV
460 470 480 490 500
LEDSPAAKEG LEEGDQILRV NNVDFTNIIR EEAVLFLLDL PKGEEVTILA
510 520 530 540 550
QKKKDVYRRI VESDVGDSFY IRTHFEYEKE SPYGLSFNKG EVFRVVDTLY
560 570 580 590 600
NGKLGSWLAI RIGKNHKEVE RGIIPNKNRA EQLASVQYTL PKTAGGDRAD
610 620 630 640 650
FWRFRGLRSS KRNLRKSRED LSAQPVQTKF PAYERVVLRE AGFLRPVTIF
660 670 680 690 700
GPIADVAREK LAREEPDIYQ IAKSEPRDAG TDQRSSGIIR LHTIKQIIDQ
710 720 730 740 750
DKHALLDVTP NAVDRLNYAQ WYPIVVFLNP DSKQGVKTMR MRLCPESRKS
760 770 780 790 800
ARKLYERSHK LRKNNHHLFT TTINLNSMND GWYGALKEAI QQQQNQLVWV
810 820 830 840 850
SEGKADGATS DDLDLHDDRL SYLSAPGSEY SMYSTDSRHT SDYEDTDTEG
860 870 880 890 900
GAYTDQELDE TLNDEVGTPP ESAITRSSEP VREDSSGMHH ENQTYPPYSP
910 920 930 940 950
QAQPQPIHRI DSPGFKPASQ QKAEASSPVP YLSPETNPAS STSAVNHNVN
960 970 980 990 1000
LTNVRLEEPT PAPSTSYSPQ ADSLRTPSTE AAHIMLRDQE PSLSSHVDPT
1010 1020 1030 1040 1050
KVYRKDPYPE EMMRQNHVLK QPAVSHPGHR PDKEPNLTYE PQLPYVEKQA
1060 1070 1080 1090 1100
SRDLEQPTYR YESSSYTDQF SRNYEHRLRY EDRVPMYEEQ WSYYDDKQPY
1110 1120 1130 1140 1150
PSRPPFDNQH SQDLDSRQHP EESSERGYFP RFEEPAPLSY DSRPRYEQAP
1160 1170 1180 1190 1200
RASALRHEEQ PAPGYDTHGR LRPEAQPHPS AGPKPAESKQ YFEQYSRSYE
1210 1220 1230 1240 1250
QVPPQGFTSR AGHFEPLHGA AAVPPLIPSS QHKPEALPSN TKPLPPPPTQ
1260 1270 1280 1290 1300
TEEEEDPAMK PQSVLTRVKM FENKRSASLE TKKDVNDTGS FKPPEVASKP
1310 1320 1330 1340 1350
SGAPIIGPKP TSQNQFSEHD KTLYRIPEPQ KPQLKPPEDI VRSNHYDPEE
1360 1370 1380 1390 1400
DEEYYRKQLS YFDRRSFENK PPAHIAASHL SEPAKPAHSQ NQSNFSSYSS
1410 1420 1430 1440 1450
KGKPPEADGV DRSFGEKRYE PIQATPPPPP LPSQYAQPSQ PVTSASLHIH
1460 1470 1480 1490 1500
SKGAHGEGNS VSLDFQNSLV SKPDPPPSQN KPATFRPPNR EDTAQAAFYP
1510 1520 1530 1540 1550
QKSFPDKAPV NGTEQTQKTV TPAYNRFTPK PYTSSARPFE RKFESPKFNH
1560 1570 1580 1590 1600
NLLPSETAHK PDLSSKTPTS PKTLVKSHSL AQPPEFDSGV ETFSIHAEKP
1610 1620 1630 1640 1650
KYQINNISTV PKAIPVSPSA VEEDEDEDGH TVVATARGIF NSNGGVLSSI
1660 1670 1680 1690 1700
ETGVSIIIPQ GAIPEGVEQE IYFKVCRDNS ILPPLDKEKG ETLLSPLVMC
1710 1720 1730 1740
GPHGLKFLKP VELRLPHCDP KTWQNKCLPG DPNYLVGANC VSVLIDHF

Background

Function:

TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed:7798316, PubMed:9792688). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells (PubMed:21240187). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (PubMed:20930113). With TJP2 and TJP3, participates to the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity).

Post-translational Modifications:

Phosphorylated at tyrosine redidues in response to epidermal growth factor (EGF) (PubMed:19332538, PubMed:7542259). This response is dependent on an intact actin microfilament system (PubMed:7542259). Dephosphorylated by PTPRJ (PubMed:19332538).

Subcellular Location:

Plasma membrane;

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

Homodimer (PubMed:17928286). Forms heterodimers (via the PDZ2 domain) with TJP2 and TJP3 (PubMed:9792688, PubMed:17928286, PubMed:16737969). Interacts with OCLN, CALM, claudins, CGN/cingulin, CXADR, GJA12, GJD3 and UBN1 (PubMed:7798316, PubMed:11734628, PubMed:12023291, PubMed:12154091, PubMed:15183511, PubMed:18823282, PubMed:20200156). Interacts (via ZU5 domain) with CDC42BPB and MYZAP (PubMed:20093627, PubMed:21240187). Interacts (via PDZ domain) with GJA1 (PubMed:18636092). Interacts (via PDZ domains) with ANKRD2 (PubMed:22016770). Interacts with BVES (via the C-terminus cytoplasmic tail) (By similarity). Interacts with HSPA4 and KIRREL1 (By similarity). Interacts with DLL1 (By similarity). Interacts with USP53 (via the C-terminal region) (By similarity). Interacts (via ABR region) with F-actin (PubMed:9792688, PubMed:12354695, PubMed:20930113).

Similarity:

The 244-aa domain between residues 633 and 876 is the primary occludin (OCLN)-binding site and is required for stable association with the tight junction (PubMed:9792688).The C-terminal region (residues 1151-1372) is an actin-binding region (ABR) that interacts directly with F-actin and plays an important role in the localization of TJP1 at junctions (PubMed:9792688, PubMed:12354695, PubMed:20930113). The ABR is also required for the localization to puncta at the free edge of cells before initiation of cell-cell contact (PubMed:12354695). The ABR is also necessary for TJP1 recruitment to podosomes (PubMed:20930113).The second PDZ domain (PDZ2) mediates homodimerization and heterodimerization with TJP2 and TJP3 (PubMed:9792688, PubMed:17928286).Belongs to the MAGUK family.

Research Fields

Research Fields:

· Cellular Processes > Cellular community - eukaryotes > Tight junction.(View pathway)
· Cellular Processes > Cellular community - eukaryotes > Gap junction.(View pathway)
· Cellular Processes > Cellular community - eukaryotes > Adherens junction.(View pathway)
· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.
· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.
· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

Reference Citations:

1). Wu C et al. Cell-Laden Electroconductive Hydrogel Simulating Nerve Matrix To Deliver Electrical Cues and Promote Neurogenesis. ACS Appl Mater Interfaces 2019 Jun 26;11(25):22152-22163 (PubMed: 31194504) [IF=8.456]

2). Wu X et al. NDP-MSH binding melanocortin-1 receptor ameliorates neuroinflammation and BBB disruption through CREB/Nr4a1/NF-κB pathway after intracerebral hemorrhage in mice. J Neuroinflammation 2019 Oct 28;16(1):192 (PubMed: 31660977) [IF=5.700]

3). Li C et al. Oxyberberine, a novel gut microbiota-mediated metabolite of berberine, possesses superior anti-colitis effect: Impact on intestinal epithelial barrier, gut microbiota profile and TLR4-MyD88-NF-κB pathway. Pharmacol Res 2020 Feb;152:104603 (PubMed: 31863867) [IF=5.574]

4). Shi J et al. Chemical features of the oligochitosan-glycated caseinate digest and its enhanced protection on barrier function of the acrylamide-injured IEC-6 cells. Food Chem 2019 Aug 30;290:246-254 (PubMed: 31000044) [IF=5.399]

5). Wu J et al. Patchouli alcohol attenuates 5-fluorouracil-induced intestinal mucositis via TLR2/MyD88/NF-kB pathway and regulation of microbiota. Biomed Pharmacother 2020 Jan 28;124:109883 (PubMed: 32004938) [IF=3.743]

6). Wang J et al. Blend of organic acids and medium chain fatty acids prevents the inflammatory response and intestinal barrier dysfunction in mice challenged with enterohemorrhagic Escherichia coli O157:H7. Int Immunopharmacol 2018 May;58:64-71 (PubMed: 29555328) [IF=3.361]

7). Zhou D et al. Astrocytes-Derived VEGF Exacerbates the Microvascular Damage of Late Delayed RBI. Neuroscience 2019 Mar 22 (PubMed: 30910640) [IF=3.244]

8). Shi J et al. Effect of caseinate glycation with oligochitosan and transglutaminase on the intestinal barrier function of the tryptic caseinate digest in IEC-6 cells. Food Funct 2019 Feb 20;10(2):652-664 (PubMed: 30652176) [IF=3.241]

9). Deng Z et al. FSGHF3 and peptides, prepared from fish skin gelatin, exert a protective effect on DSS-induced colitis via the Nrf2 pathway. Food Funct 2019 Dec 11 (PubMed: 31825438) [IF=3.241]

10). Shi J et al. Influence of the Maillard-type caseinate glycation with lactose on the intestinal barrier activity of the caseinate digest in IEC-6 cells. Food Funct 2019 Mar 25 (PubMed: 30906943) [IF=3.241]

11). Zhao Deng et al. FSGHF3 and peptides, prepared from fish skin gelatin, exert a protective effect on DSS-induced colitis via the Nrf2 pathway† . FOOD FUNCT 2019 Dec [IF=3.241]

12). Wang HF et al. Crocetin inhibits the proliferation, migration and TGF-β2-induced epithelial-mesenchymal transition of retinal pigment epithelial cells. Eur J Pharmacol 2017 Nov 15;815:391-398 (PubMed: 28970011) [IF=3.170]

Application: WB    Species:human;    Sample:ARPE-19

Figure 4. Crocetin inhibits TGF-β2-induced EMT. After 1 h of pretreatment with crocetin, ARPE-19 cells used for EMT assay were stimulated with or without recombinant human TGF-β2 for up to 24 or 48 h. (A) Phase contrast photomicrographs of confluent cultures of cells were captured after treatment for 48 h. Scale bar: 200 μm. (B) Western blot analysis levels of of ZO-1, E-cadherin, Vimentin, α-SMA and the housekeeping protein GAPDH in the lysates of ARPE-19 cells after treatment for 48 h. *P< 0.05, **P< 0.01, ***P< 0.001. The data are presented as the mean ± S.D. (n = 3/group).


13). Feng Y et al. Chronic colitis induces meninges traffic of gut-derived T cells, unbalances M1 and M2 microglia/macrophage and increases ischemic brain injury in mice. Brain Res 2018 Nov 13 (PubMed: 30445026)

14). Sun R et al. MiR-429 regulates blood-spinal cord barrier permeability by targeting Krüppel-like factor 6. Biochem Biophys Res Commun 2020 Mar 4 (PubMed: 32145913)

15). Zou Y et al. Oregano Essential Oil Improves Intestinal Morphology and Expression of Tight Junction Proteins Associated with Modulation of Selected Intestinal Bacteria and Immune Status in a Pig Model. Biomed Res Int 2016;2016:5436738 (PubMed: 27314026)

Application: WB    Species:pig;    Sample:jejunum

Figure 4: Effect of OEO on the ZO-1 and occludin levels in the jejunum of pig. (a) ZO-1 mRNA level, (b) occludin mRNA level, (c) ZO-1 protein level, and (d) occludin protein level. Expression of the selected genes was quantified by quantitative reverse transcription-PCR. Equal loading was assessed by


16). Zhong X et al. Scutellarin-treated exosomes increase claudin 5, occludin and ZO1 expression in rat brain microvascular endothelial cells. Exp Ther Med 2019 Jul;18(1):33-40 (PubMed: 31258635)

17). Zhong X et al. Scutellarin-treated exosomes increase claudin 5, occludin and ZO1 expression in rat brain microvascular endothelial cells. Exp Ther Med 2019 Jul;18(1):33-40 (PubMed: 31258635)

18). Yang X et al. Angiopoietin-Like Protein 4 (ANGPTL4) Induces Retinal Pigment Epithelial Barrier Breakdown by Activating Signal Transducer and Activator of Transcription 3 (STAT3): Evidence from ARPE-19 Cells Under Hypoxic Condition and Diabetic Rats. Med Sci Monit 2019 Sep 8;25:6742-6754 (PubMed: 31494661)

19). Gong Q et al. Enhanced ROBO4 is mediated by up-regulation of HIF-1α/SP1 or reduction in miR-125b-5p/miR-146a-5p in diabetic retinopathy. J Cell Mol Med 2019 May 15 (PubMed: 31094072)

20). Hui-Fang Wang et al. Crocetin inhibits the proliferation, migration and TGF-β 2-induced epithelial-mesenchymal transition of retinal pigment epithelial cells . EUR J PHARMACOL 2017 Nov;815:391-398

21). et al. Protective Effects of the Soluble Epoxide Hydrolase Inhibitor 1-Trifluoromethoxyphenyl-3-(1-Propionylpiperidin-4-yl) Urea in a Rat Model of Permanent Middle Cerebral Artery Occlusion.

22). Jia Shi et al. Effects of Maillard-type caseinate glycation on the preventive action of caseinate digests in acrylamide-induced intestinal barrier dysfunction in IEC-6 cells. RSC Advances 2018

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Catalog Number :

AF5145-BP
(Blocking peptide available as AF5145-BP)

Price/Size :

$200/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Dog
92%
Horse
85%
Chicken
77%
Rabbit
77%
Pig
62%
Bovine
46%
Sheep
46%
Zebrafish
0%
Xenopus
0%
High similarity Medium similarity Low similarity No similarity
Q07157 as Substrate
Site PTM Type Enzyme
T16 Phosphorylation
S53 Phosphorylation
S117 Phosphorylation
S125 Phosphorylation
S131 Phosphorylation
Y132 Phosphorylation
S141 Phosphorylation
S144 Phosphorylation
S151 Phosphorylation
K153 Ubiquitination
S160 Phosphorylation
S162 Phosphorylation
S166 Phosphorylation
S168 Phosphorylation
S175 Phosphorylation
S178 Phosphorylation
S179 Phosphorylation
K183 Ubiquitination
T185 Phosphorylation
K194 Ubiquitination
S212 Phosphorylation
S215 Phosphorylation
S241 Phosphorylation
T267 Phosphorylation
S275 Phosphorylation
S277 Phosphorylation
S280 Phosphorylation
S284 Phosphorylation
S290 Phosphorylation
S294 Phosphorylation
S297 Phosphorylation
S300 Phosphorylation
S313 Phosphorylation
S315 Phosphorylation
S320 Phosphorylation
S323 Phosphorylation
S329 Phosphorylation
S334 Phosphorylation
S337 Phosphorylation
S340 Phosphorylation
S347 Phosphorylation
K348 Ubiquitination
S353 Phosphorylation
T354 Phosphorylation
T363 Phosphorylation
T379 Phosphorylation
S381 Phosphorylation
Y389 Phosphorylation
S402 Phosphorylation
S421 Phosphorylation
S432 Phosphorylation
T476 Phosphorylation
S513 Phosphorylation
Y520 Phosphorylation
T548 Phosphorylation
Y550 Phosphorylation
K553 Ubiquitination
S585 Phosphorylation
Y588 Phosphorylation
T589 Phosphorylation
K592 Ubiquitination
S617 Phosphorylation
S622 Phosphorylation
T628 Phosphorylation
Y633 Phosphorylation
T648 Phosphorylation
Y669 Phosphorylation
K673 Ubiquitination
S686 Phosphorylation
K695 Ubiquitination
K702 Ubiquitination
T709 Phosphorylation
Y718 Phosphorylation
K733 Acetylation
K737 Acetylation
T738 Phosphorylation
T770 Phosphorylation
T772 Phosphorylation
T809 Phosphorylation
S810 Phosphorylation
S821 Phosphorylation
Y822 Phosphorylation
S824 Phosphorylation
S828 Phosphorylation
Y830 Phosphorylation
S831 Phosphorylation
Y833 Phosphorylation
S834 Phosphorylation
S837 Phosphorylation
Y843 Phosphorylation
T854 Phosphorylation
T861 Phosphorylation
T868 Phosphorylation
S877 Phosphorylation
S886 Phosphorylation
T894 Phosphorylation
Y895 Phosphorylation
Y898 Phosphorylation
S899 Phosphorylation
S912 Phosphorylation
S919 Phosphorylation
S926 Phosphorylation
S927 Phosphorylation
Y931 Phosphorylation
S933 Phosphorylation
T936 Phosphorylation
S964 Phosphorylation
T965 Phosphorylation
S966 Phosphorylation
Y967 Phosphorylation
S968 Phosphorylation
S973 Phosphorylation
T976 Phosphorylation
S978 Phosphorylation
T979 Phosphorylation
S992 Phosphorylation
S994 Phosphorylation
S1025 Phosphorylation
Y1039 Phosphorylation
S1051 Phosphorylation
T1058 Phosphorylation
Y1059 Phosphorylation
Y1061 Phosphorylation
S1064 Phosphorylation
S1065 Phosphorylation
Y1066 Phosphorylation
T1067 Phosphorylation
S1071 Phosphorylation
Y1074 Phosphorylation
Y1087 Phosphorylation
S1092 Phosphorylation
S1102 Phosphorylation
S1111 Phosphorylation
Y1128 Phosphorylation
S1139 Phosphorylation
Y1140 Phosphorylation
S1142 Phosphorylation
Y1146 Phosphorylation
S1153 Phosphorylation
Y1165 Phosphorylation
T1167 Phosphorylation
S1180 Phosphorylation
K1189 Ubiquitination
Y1191 Phosphorylation
Y1195 Phosphorylation
S1196 Phosphorylation
S1198 Phosphorylation
Y1199 Phosphorylation
K1269 Ubiquitination
S1278 Phosphorylation
K1321 Ubiquitination
T1322 Phosphorylation
Y1324 Phosphorylation
K1331 Ubiquitination
Y1346 Phosphorylation
Y1354 Phosphorylation
Y1355 Phosphorylation
K1357 Ubiquitination
Y1361 Phosphorylation
S1366 Phosphorylation
S1397 Phosphorylation
Y1398 Phosphorylation
S1399 Phosphorylation
S1413 Phosphorylation
K1417 Acetylation
Y1419 Phosphorylation
T1425 Phosphorylation
S1433 Phosphorylation
Y1435 Phosphorylation
S1439 Phosphorylation
T1443 Phosphorylation
S1444 Phosphorylation
S1446 Phosphorylation
S1451 Phosphorylation
S1503 Phosphorylation
T1521 Phosphorylation
Y1524 Phosphorylation
T1528 Phosphorylation
K1530 Methylation
Y1532 Phosphorylation
S1534 Phosphorylation
S1545 Phosphorylation
T1567 Phosphorylation
T1569 Phosphorylation
S1570 Phosphorylation
T1573 Phosphorylation
S1577 Phosphorylation
S1579 Phosphorylation
S1588 Phosphorylation
S1594 Phosphorylation
K1601 Ubiquitination
Y1602 Phosphorylation
S1617 Phosphorylation
S1619 Phosphorylation
S1655 Phosphorylation
K1687 Ubiquitination
K1709 Ubiquitination
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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