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  • Product Name
    His-Tag Rabbit pAb
  • Catalog No.
    T0051
  • Source
    Rabbit
  • Application
    WB,ELISA
  • Reactivity
    All
  • Concentration
    1mg/ml

Product Information

Applications:

WB: 1:3000~1:10000, ELISA(peptide) 1:20000-1:40000

Reactivity:

All

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and glycerol.

Immunogen Information

Immunogen:

Rabbit polyclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

Molecular Weight:

Observed Mol.Wt.: Not available.
Predicted Mol.Wt.: Not available.

Subcellular Location:

Endoplasmic reticulum membrane. Microsome membrane.

Description:

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.

Western blot analysis of His-Tag Rabbit pAb expression in His fusion protein sample

Reference Citations:

1). Rao XJ et al. Characterization of a dual-CRD galectin in the silkworm Bombyx mori. Dev Comp Immunol 2016 Jul;60:149-59 (PubMed: 26944801)

Application: WB    Species:Not available;    Sample:Not available;


2). Zhan MY et al. A single-CRD C-type lectin is important for bacterial clearance in the silkworm. Dev Comp Immunol 2016 Dec;65:330-339 (PubMed: 27519466)

3). Cao X et al. Expression and characterization of recombinant humanized anti-HER2 single-chain antibody in Pichia pastoris for targeted cancer therapy. Biotechnol Lett 2015 Jul;37(7):1347-54 (PubMed: 25735855)

Application: IF/ICC    Species:Not available;    Sample:Not available;


4). Shahzad T et al. Molecular cloning and analysis of a C-type lectin from silkworm Bombyx mori. Arch Insect Biochem Physiol 2017 Jul;95(3) (PubMed: 28618068)

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Catalog Number :

T0051-BP

Price/Size :

$200/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.