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$150 50ul
$250 100ul
$350 200ul
$1200 1ml

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  • Product Name
    HA-Tag Rabbit pAb
  • Catalog No.
    T0050
  • RRID
    AB_2837583
  • Source
    Rabbit
  • Application
    WB,IP,CoIP,ELISA(peptide)
  • Reactivity
    All
  • Concentration
    1mg/ml

Product Information

Applications:

WB: 1:3000-1:10000, IP 1:200, CoIP 1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

All

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

The HA tag antibody can recognize C-terminal, internal, and N-terminal HA-tag fusion proteins.

RRID:

AB_2837583
Please cite this product as: Affinity Biosciences Cat# T0050, RRID:AB_2837583.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and glycerol.

Immunogen Information in 3D

Immunogen:

Rabbit polyclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

Molecular Weight:

Observed Mol.Wt.: Not available.
Predicted Mol.Wt.: Not available.

Subcellular Location:

Endoplasmic reticulum membrane. Microsome membrane.

Description:

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.HA tag antibody is a highly sensitive and affinity monoclonal antibody applicable to HA-tagged fusion protein detection.

Reference Citations:

1). Lei R et al. bHLH121 Functions as A Direct Link that Facilitates the Activation of FIT by bHLH IVc Transcription Factors for Maintaining Fe Homeostasis in Arabidopsis. Mol Plant 2020 Jan 18 (PubMed: 31962167) [IF=12.084]

2). Zhang H et al. Oryza sativa POSITIVE REGULATOR OF IRON DEFICIENCY RESPONSE 2 (OsPRI2) and OsPRI3 are involved in the maintenance of Fe homeostasis. Plant Cell Environ 2020 Jan;43(1):261-274 (PubMed: 31674679) [IF=6.362]

3). Liang G;Zhang H;Li Y;Pu M;Yang Y;Li C;Lu C;Xu P;Yu D; et al. Oryza sativa FER‐LIKE FE DEFICIENCY‐INDUCED TRANSCRIPTION FACTOR (OsFIT/OsbHLH156) interacts with OsIRO2 to regulate iron homeostasis. J Integr Plant Biol 2020 May;62(5):668-689. (PubMed: 32237201) [IF=4.885]

4). Sun L et al. Proteasome inhibition promotes mono-ubiquitination and nuclear translocation of mature (52 kDa) PINK1. Biochem Biophys Res Commun 2019 Sep 17;517(2):376-382 (PubMed: 31362890)

5). Xie M;Yin Y;Chen L;Yin A;Liu Y;Liu Y;Dong L;Lai Q;Zhou J;Zhang L;Xu M;Chen Z;Zuo D; et al. Scavenger receptor A impairs interferon response to HBV infection by limiting TRAF 3 ubiquitination through recruiting OTUB 1. FEBS J 2020 Jan;287(2):310-324. (PubMed: 31386800)

6). Xie M;Yin Y;Chen L;Yin A;Liu Y;Liu Y;Dong L;Lai Q;Zhou J;Zhang L;Xu M;Chen Z;Zuo D; et al. Scavenger receptor A impairs interferon response to HBV infection by limiting TRAF 3 ubiquitination through recruiting OTUB 1. FEBS J 2020 Jan;287(2):310-324. (PubMed: 31386800)

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Catalog Number :

T0050-BP
(Blocking peptide available as T0050-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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