CDC42BPA Antibody - #DF4786

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Product: CDC42BPA Antibody
Catalog: DF4786
Description: Rabbit polyclonal antibody to CDC42BPA
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 97 KD; 197kD(Calculated).
Uniprot: Q5VT25
RRID: AB_2837140

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(91%)
Clonality:
Polyclonal
Specificity:
CDC42BPA Antibody detects endogenous levels of total CDC42BPA.
RRID:
AB_2837140
Cite Format: Affinity Biosciences Cat# DF4786, RRID:AB_2837140.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A930014J19Rik; CDC42 binding protein kinase alpha (DMPK like); CDC42 binding protein kinase alpha; CDC42 binding protein kinase alpha dmpk like; CDC42 binding protein kinase beta; CDC42-binding protein kinase alpha; CDC42BPA; DKFZp686L1738; DKFZp686P1738; DMPK like; DMPK-like alpha; FLJ23347; KIAA0451; MGC189823; MRCK alpha; mrck; mrcka; MRCKA_HUMAN; Myotonic dystrophy kinase related CDC42 binding protein kinase alpha; Myotonic dystrophy kinase-related CDC42-binding kinase alpha; Myotonic dystrophy protein kinase-like alpha; OTTHUMP00000035727; OTTHUMP00000035728; OTTHUMP00000035729; OTTHUMP00000035730; pk428; RP5-1087E8.4; Ser thr protein kinase PK428; Ser thr protein kinase related to the myotonic dystrophy protein kinase; Serine/threonine protein kinase MRCK alpha; Serine/threonine-protein kinase MRCK alpha;

Immunogens

Immunogen:
Uniprot:

Q5VT25(MRCKA_HUMAN) >>Visit HPA database.

Gene(ID):
CDC42BPA(8476)
Expression:
Q5VT25 MRCKA_HUMAN:

Abundant in the heart, brain, skeletal muscle, kidney, and pancreas, with little or no expression in the lung and liver.

Sequence:
MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
91
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q5VT25 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
R172 Methylation
R179 Methylation
S222 Phosphorylation Q5VT25 (CDC42BPA)
S234 Phosphorylation Q5VT25 (CDC42BPA)
T240 Phosphorylation Q5VT25 (CDC42BPA)
K338 Ubiquitination
K339 Ubiquitination
T403 Phosphorylation Q5VT25 (CDC42BPA)
Y444 Phosphorylation
K455 Ubiquitination
K461 Ubiquitination
Y474 Phosphorylation
S475 Phosphorylation
K490 Ubiquitination
K531 Ubiquitination
K669 Acetylation
K669 Ubiquitination
S674 Phosphorylation
S679 Phosphorylation
T687 Phosphorylation
S725 Phosphorylation
S750 Phosphorylation
K785 Ubiquitination
S843 Phosphorylation
T846 Phosphorylation
S856 Phosphorylation
T862 Phosphorylation
K893 Ubiquitination
K913 Ubiquitination
K920 Ubiquitination
S940 Phosphorylation
S989 Phosphorylation
T992 Phosphorylation
T994 Phosphorylation
S1003 Phosphorylation
K1079 Ubiquitination
K1122 Ubiquitination
S1171 Phosphorylation
S1173 Phosphorylation
T1185 Phosphorylation
K1217 Ubiquitination
K1228 Ubiquitination
K1307 Ubiquitination
K1312 Ubiquitination
S1378 Phosphorylation
T1505 Phosphorylation
Y1510 Phosphorylation
Y1544 Phosphorylation
S1545 Phosphorylation
S1604 Phosphorylation
S1611 Phosphorylation
S1613 Phosphorylation
S1616 Phosphorylation
T1618 Phosphorylation
S1629 Phosphorylation
S1632 Phosphorylation
S1635 Phosphorylation
S1639 Phosphorylation
S1644 Phosphorylation
S1651 Phosphorylation
S1654 Phosphorylation
S1656 Phosphorylation
S1664 Phosphorylation
S1669 Phosphorylation
S1693 Phosphorylation
S1700 Phosphorylation
S1703 Phosphorylation
S1706 Phosphorylation
S1710 Phosphorylation
S1719 Phosphorylation
S1721 Phosphorylation
S1724 Phosphorylation
T1725 Phosphorylation
S1729 Phosphorylation

PTMs - Q5VT25 As Enzyme

Substrate Site Source
P10916 (MYL2) S19 Uniprot
P24844 (MYL9) T19 Uniprot
P24844 (MYL9) S20 Uniprot
P26038 (MSN) T558 Uniprot
P53667-1 (LIMK1) T508 Uniprot
P53671-2 (LIMK2) T505 Uniprot
P53671-1 (LIMK2) T526 Uniprot
Q5VT25 (CDC42BPA) S222 Uniprot
Q5VT25 (CDC42BPA) S234 Uniprot
Q5VT25 (CDC42BPA) T240 Uniprot
Q5VT25 (CDC42BPA) T403 Uniprot
Q9BZL4 (PPP1R12C) T560 Uniprot

Research Backgrounds

Function:

Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates: PPP1R12A, LIMK1 and LIMK2. May play a role in TFRC-mediated iron uptake. In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (By similarity). Triggers the formation of an extrusion apical actin ring required for epithelial extrusion of apoptotic cells.

PTMs:

Proteolytically cleaved by caspases upon apoptosis induction. The cleavage at Asp-478 by CASP3 increases its kinase activity (in vitro).

Subcellular Location:

Cytoplasm. Cell projection>Lamellipodium.
Note: Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent. Localizes in the lamellipodium in a FAM89B/LRAP25-dependent manner.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Abundant in the heart, brain, skeletal muscle, kidney, and pancreas, with little or no expression in the lung and liver.

Subunit Structure:

Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42. Forms a tripartite complex with MYO18A and LURAP1 with the latter acting as an adapter connecting CDC42BPA and MYO18A. LURAP1 binding results in activation of CDC42BPA by abolition of its negative autoregulation. Interacts with LURAP1. Interacts (via AGC-kinase C-terminal domain) with FAM89B/LRAP25 (via LRR repeat). Forms a tripartite complex with FAM89B/LRAP25 and LIMK1 (By similarity).

Family&Domains:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.

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