EME1 Antibody - #DF4671

(1)  
Product: EME1 Antibody
Catalog: DF4671
Description: Rabbit polyclonal antibody to EME1
Application: WB IF/ICC
Reactivity: Human
Prediction: Rabbit, Dog
Mol.Wt.: 65 KD; 63kD(Calculated).
Uniprot: Q96AY2
RRID: AB_2837022

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Rabbit(100%), Dog(92%)
Clonality:
Polyclonal
Specificity:
EME1 Antibody detects endogenous levels of total EME1.
RRID:
AB_2837022
Cite Format: Affinity Biosciences Cat# DF4671, RRID:AB_2837022.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

6820428D13; Crossover junction endonuclease EME1; EC 3.1.22.-; EME1; Eme1 essential meiotic endonuclease 1 homolog 1 (S. pombe); EME1, S. pombe, homolog of, 1; EME1_HUMAN; essential meiotic endonuclease 1 homolog 1 (S pombe); Essential Meiotic Endonuclease 1 Homolog 1; essential meiotic endonuclease 1 homolog 2; Essential meiotic endonuclease 1, S. pombe, homolog of, 1; FLJ31364; hMMS4; homolog of yeast EME1 endonuclease; MGC106543; MMS4; MMS4 homolog; MMS4L;

Immunogens

Immunogen:
Uniprot:

Q96AY2(EME1_HUMAN) >>Visit HPA database.

Gene(ID):
EME1(146956)
Sequence:
MALKKSSPSLDSGDSDSEELPTFAFLKKEPSSTKRRQPEREEKIVVVDISDCEASCPPAPELFSPPVPEIAETVTQTQPVRLLSSESEDEEEFIPLAQRLTCKFLTHKQLSPEDSSSPVKSVLDHQNNEGASCDWKKPFPKIPEVPLHDTPERSAADNKDLILDPCCQLPAYLSTCPGQSSSLAVTKTNSDILPPQKKTKPSQKVQGRGSHGCRQQRQARQKESTLRRQERKNAALVTRMKAQRPEECLKHIIVVLDPVLLQMEGGGQLLGALQTMECRCVIEAQAVPCSVTWRRRAGPSEDREDWVEEPTVLVLLRAEAFVSMIDNGKQGSLDSTMKGKETLQGFVTDITAKTAGKALSLVIVDQEKCFSAQNPPRRGKQGANKQTKKQQQRQPEASIGSMVSRVDAEEALVDLQLHTEAQAQIVQSWKELADFTCAFTKAVAEAPFKKLRDETTFSFCLESDWAGGVKVDLAGRGLALVWRRQIQQLNRVSLEMASAVVNAYPSPQLLVQAYQQCFSDKERQNLLADIQVRRGEGVTSTSRRIGPELSRRIYLQMTTLQPHLSLDSAD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Dog
92
Horse
77
Pig
67
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96AY2 As Substrate

Site PTM Type Enzyme
S6 Phosphorylation
S7 Phosphorylation
S9 Phosphorylation
S12 Phosphorylation
S15 Phosphorylation
S17 Phosphorylation
T33 Phosphorylation
S64 Phosphorylation
S84 Phosphorylation
S85 Phosphorylation
S87 Phosphorylation
K103 Sumoylation
S111 Phosphorylation
S117 Phosphorylation
S132 Phosphorylation
K136 Sumoylation
T150 Phosphorylation
K232 Sumoylation
K232 Ubiquitination
T238 Phosphorylation
K241 Ubiquitination
K329 Ubiquitination
S332 Phosphorylation
T336 Phosphorylation
K338 Methylation
K338 Sumoylation
K340 Methylation
K340 Ubiquitination
K353 Ubiquitination
K357 Ubiquitination
K441 Ubiquitination
K449 Ubiquitination

Research Backgrounds

Function:

Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.

Subcellular Location:

Nucleus>Nucleolus.
Note: Recruited to regions of DNA damage in S-phase cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

May self-associate. Interacts with MUS81.

Family&Domains:

Belongs to the EME1/MMS4 family.

Research Fields

· Genetic Information Processing > Replication and repair > Homologous recombination.

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.