ME1 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/4

Western blot analysis of extracts from rat spleen, using ME1 Antibody.

DF4217 at 1/100 staining Rat liver tissue by IHC-P.

DF4217 staining Hela cells by IF/ICC.

DF4217 staining A549 cells by IF/ICC.

Product:	ME1 Antibody
Catalog:	DF4217
Description:	Rabbit polyclonal antibody to ME1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.:	64 KD; 64kD(Calculated).
Uniprot:	P48163
RRID:	AB_2836568

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(91%), Rabbit(100%)

Clonality:

Polyclonal

Specificity:

ME1 Antibody detects endogenous levels of total ME1.

RRID:

AB_2836568
Cite Format: Affinity Biosciences Cat# DF4217, RRID:AB_2836568.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Cytosolic malic enzyme 1; HUMNDME; Malate dehydrogenase; Malic enzyme 1; Malic enzyme 1, NADP(+) dependent, cytosolic; Malic enzyme 1, soluble; Malic enzyme, cytoplasmic; MAOX_HUMAN; ME1; MES; NADP dependent malic enzyme; NADP ME; NADP-dependent malic enzyme; NADP-ME; Pyruvic malic carboxylase;

Immunogens

Immunogen:

Uniprot:

P48163(MAOX_HUMAN) >>Visit HPA database.

Gene(ID):

ME1(4199)

Expression:

P48163 MAOX_HUMAN:

Expressed in all tissues tested including liver, placenta and white adipose tissue.

Sequence:

P48163 MAOX_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEPEAPRRRHTHQRGYLLTRNPHLNKDLAFTLEERQQLNIHGLLPPSFNSQEIQVLRVVKNFEHLNSDFDRYLLLMDLQDRNEKLFYRVLTSDIEKFMPIVYTPTVGLACQQYSLVFRKPRGLFITIHDRGHIASVLNAWPEDVIKAIVVTDGERILGLGDLGCNGMGIPVGKLALYTACGGMNPQECLPVILDVGTENEELLKDPLYIGLRQRRVRGSEYDDFLDEFMEAVSSKYGMNCLIQFEDFANVNAFRLLNKYRNQYCTFNDDIQGTASVAVAGLLAALRITKNKLSDQTILFQGAGEAALGIAHLIVMALEKEGLPKEKAIKKIWLVDSKGLIVKGRASLTQEKEKFAHEHEEMKNLEAIVQEIKPTALIGVAAIGGAFSEQILKDMAAFNERPIIFALSNPTSKAECSAEQCYKITKGRAIFASGSPFDPVTLPNGQTLYPGQGNNSYVFPGVALGVVACGLRQITDNIFLTTAEVIAQQVSDKHLEEGRLYPPLNTIRDVSLKIAEKIVKDAYQEKTATVYPEPQNKEAFVRSQMYSTDYDQILPDCYSWPEEVQKIQTKVDQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Rabbit

100

Sheep

Zebrafish

Dog

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P48163 As Substrate

P48163

Site	PTM Type	Source
M1	Acetylation	Uniprot
K26	Ubiquitination	Uniprot
K60	Acetylation	Uniprot
K60	Ubiquitination	Uniprot
S67	Phosphorylation	Uniprot
K84	Acetylation	Uniprot
K84	Ubiquitination	Uniprot
S92	Phosphorylation	Uniprot
Y113	Phosphorylation	Uniprot
T151	Phosphorylation	Uniprot
Y208	Phosphorylation	Uniprot
S336	Phosphorylation	Uniprot
K337	Ubiquitination	Uniprot
K351	Ubiquitination	Uniprot
K353	Ubiquitination	Uniprot
K412	Ubiquitination	Uniprot
K422	Ubiquitination	Uniprot
T474	Phosphorylation	Uniprot
K492	Ubiquitination	Uniprot
S510	Phosphorylation	Uniprot
K512	Ubiquitination	Uniprot
K516	Ubiquitination	Uniprot
K519	Ubiquitination	Uniprot
K525	Ubiquitination	Uniprot
K536	Ubiquitination	Uniprot
K569	Sumoylation	Uniprot
K569	Ubiquitination	Uniprot

Research Backgrounds

P48163_MAOX_HUMAN

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Expressed in all tissues tested including liver, placenta and white adipose tissue.

Subunit Structure:

Homotetramer.

Family&Domains:

Belongs to the malic enzymes family.

Research Fields

· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

ME1 Antibody - #DF4217