GNPAT Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from Rat brain, using GNPAT Antibody at 1/1000 dilution.

Product:	GNPAT Antibody
Catalog:	DF3993
Description:	Rabbit polyclonal antibody to GNPAT
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Rat, Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	77 KD; 77kD(Calculated).
Uniprot:	O15228
RRID:	AB_2836353

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Rat(%), Pig(100%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

GNPAT Antibody detects endogenous levels of total GNPAT.

RRID:

AB_2836353
Cite Format: Affinity Biosciences Cat# DF3993, RRID:AB_2836353.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Acyl CoA dihydroxyacetonephosphateacyltransferase; Acyl-CoA:dihydroxyacetonephosphateacyltransferase; DAP AT; DAP-AT; DAPAT; DHAP AT; DHAP-AT; DHAPAT; Dihydroxyacetone phosphate acyltransferase; EC 2.3.1.42; Glycerone-phosphate O-acyltransferase; Glyceronephosphate O acyltransferase; GNPAT; GNPAT_HUMAN; OTTHUMP00000036147;

Immunogens

Immunogen:

Uniprot:

O15228(GNPAT_HUMAN) >>Visit HPA database.

Gene(ID):

GNPAT(8443)

Sequence:

O15228 GNPAT_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MESSSSSNSYFSVGPTSPSAVVLLYSKELKKWDEFEDILEERRHVSDLKFAMKCYTPLVYKGITPCKPIDIKCSVLNSEEIHYVIKQLSKESLQSVDVLREEVSEILDEMSHKLRLGAIRFCAFTLSKVFKQIFSKVCVNEEGIQKLQRAIQEHPVVLLPSHRSYIDFLMLSFLLYNYDLPVPVIAAGMDFLGMKMVGELLRMSGAFFMRRTFGGNKLYWAVFSEYVKTMLRNGYAPVEFFLEGTRSRSAKTLTPKFGLLNIVMEPFFKREVFDTYLVPISISYDKILEETLYVYELLGVPKPKESTTGLLKARKILSENFGSIHVYFGDPVSLRSLAAGRMSRSSYNLVPRYIPQKQSEDMHAFVTEVAYKMELLQIENMVLSPWTLIVAVLLQNRPSMDFDALVEKTLWLKGLTQAFGGFLIWPDNKPAEEVVPASILLHSNIASLVKDQVILKVDSGDSEVVDGLMLQHITLLMCSAYRNQLLNIFVRPSLVAVALQMTPGFRKEDVYSCFRFLRDVFADEFIFLPGNTLKDFEEGCYLLCKSEAIQVTTKDILVTEKGNTVLEFLVGLFKPFVESYQIICKYLLSEEEDHFSEEQYLAAVRKFTSQLLDQGTSQCYDVLSSDVQKNALAACVRLGVVEKKKINNNCIFNVNEPATTKLEEMLGCKTPIGKPATAKL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Dog

100

Rabbit

100

Bovine

Sheep

Zebrafish

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15228 As Substrate

O15228

Site	PTM Type	Source
S17	Phosphorylation	Uniprot
K31	Ubiquitination	Uniprot
K49	Ubiquitination	Uniprot
K67	Ubiquitination	Uniprot
K90	Ubiquitination	Uniprot
S111	Phosphorylation	Uniprot
K113	Ubiquitination	Uniprot
K128	Acetylation	Uniprot
K136	Ubiquitination	Uniprot
K146	Ubiquitination	Uniprot
K251	Ubiquitination	Uniprot
T291	Phosphorylation	Uniprot
K304	Ubiquitination	Uniprot
K312	Ubiquitination	Uniprot
S345	Phosphorylation	Uniprot
S346	Phosphorylation	Uniprot
S589	Phosphorylation	Uniprot
K606	Ubiquitination	Uniprot
S625	Phosphorylation	Uniprot
K643	Acetylation	Uniprot
K644	Acetylation	Uniprot
K674	Ubiquitination	Uniprot

Research Backgrounds

O15228_GNPAT_HUMAN

Subcellular Location:

Peroxisome membrane>Peripheral membrane protein>Matrix side.
Note: Exclusively localized to the lumenal side of the peroxisomal membrane.

Subunit Structure:

May be part of a heterotrimeric complex composed of DAP-AT, ADAP-S and a modified form of DAP-AT.

Family&Domains:

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.

Belongs to the GPAT/DAPAT family.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

Restrictive clause

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For Research Use Only.
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GNPAT Antibody - #DF3993