MLANA Antibody - #AF0204

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Product: MLANA Antibody
Catalog: AF0204
Description: Rabbit polyclonal antibody to MLANA
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Mol.Wt.: 15kDa; 13kD(Calculated).
Uniprot: Q16655
RRID: AB_2833288

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
MLANA Antibody detects endogenous levels of total MLANA.
RRID:
AB_2833288
Cite Format: Affinity Biosciences Cat# AF0204, RRID:AB_2833288.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Antigen LB39 AA; Antigen LB39-AA; Antigen SK29 AA; Antigen SK29-AA; MAR1_HUMAN; MART 1; MART-1; MART1; Melan A; Melan A protein; Melanoma antigen recognized by T cells 1; Melanoma antigen recognized by T-cells 1; MLAN A; MLANA; OTTHUMP00000021036; OTTHUMP00000021037; OTTHUMP00000021038; Protein Melan-A;

Immunogens

Immunogen:
Uniprot:

Q16655(MAR1_HUMAN) >>Visit HPA database.

Gene(ID):
MLANA(2315)
Expression:
Q16655 MAR1_HUMAN:

Expression is restricted to melanoma and melanocyte cell lines and retina.

Description:
MLANA Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein SILV/PMEL17, which is critical to the formation of stage II melanosomes. Interacts with SILV/PMEL17.
Sequence:
MPREDAHFIYGYPKKGHGHSYTTAEEAAGIGILTVILGVLLLIGCWYCRRRNGYRALMDKSLHVGTQCALTRRCPQEGFDHRDSKVSLQEKNCEPVVPNAPPAYEKLSAEQSPPPYSP

PTMs - Q16655 As Substrate

Site PTM Type Enzyme
S87 Phosphorylation
Y104 Phosphorylation
S108 Phosphorylation
Y116 Phosphorylation

Research Backgrounds

Function:

Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.

PTMs:

Acylated.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass type III membrane protein. Golgi apparatus. Golgi apparatus>trans-Golgi network membrane. Melanosome.
Note: Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expression is restricted to melanoma and melanocyte cell lines and retina.

Subunit Structure:

Interacts with PMEL. Interacts with GPR143.

References

1). The HIF1α-PDGFD-PDGFRα axis controls glioblastoma growth at normoxia/mild-hypoxia and confers sensitivity to targeted therapy by echinomycin. Journal of experimental & clinical cancer research : CR, 2021 (PubMed: 34470658) [IF=11.3]

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