PTPN22 Antibody - #AF0391

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Product: PTPN22 Antibody
Catalog: AF0391
Description: Rabbit polyclonal antibody to PTPN22
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 91kDa; 92kD(Calculated).
Uniprot: Q9Y2R2
RRID: AB_2833545

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(92%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
PTPN22 Antibody detects endogenous levels of total PTPN22.
RRID:
AB_2833545
Cite Format: Affinity Biosciences Cat# AF0391, RRID:AB_2833545.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Hematopoietic cell protein tyrosine phosphatase 70Z PEP; Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP; Lymphoid phosphatase; Lymphoid specific protein tyrosine phosphatase; lymphoid-specific, inducible human protein tyrosine phosphatase; Lyp 1; Lyp 2; LyP; Lyp1; Lyp2; LyPTP; OTTHUMP00000013720; OTTHUMP00000013721; OTTHUMP00000233450; OTTHUMP00000233451; PEP; PEST-domain phosphatase; Protein tyrosine phosphatase non receptor type 22 (lymphoid); Protein tyrosine phosphatase non receptor type 22; Protein tyrosine phosphatase non receptor type 8; Protein tyrosine phosphatase non receptor type 8, formerly; PTN22_HUMAN; PTPN 22; PTPN 8; Ptpn22; PTPN8; PTPN8, formerly; Tyrosine-protein phosphatase non-receptor type 22;

Immunogens

Immunogen:
Uniprot:

Q9Y2R2(PTN22_HUMAN) >>Visit HPA database.

Gene(ID):
PTPN22(26191)
Expression:
Q9Y2R2 PTN22_HUMAN:

Expressed in bone marrow, B and T-cells, PBMCs, natural killer cells, monocytes, dendritic cells and neutrophils (PubMed:15208781). Both isoform 1 and 4 are predominantly expressed in lymphoid tissues and cells. Isoform 1 is expressed in thymocytes and both mature B and T-cells.

Description:
PTPN22 Seems to act on Cbl. May play a role in regulating the function of Cbl and its associated protein kinases. Acts as negative regulator of T cell receptor (TCR) signaling. Dephosphorylates and inactivates the SRC family kinases. Belongs to the protein-tyrosine phosphatase family.
Sequence:
MDQREILQKFLDEAQSKKITKEEFANEFLKLKRQSTKYKADKTYPTTVAEKPKNIKKNRYKDILPYDYSRVELSLITSDEDSSYINANFIKGVYGPKAYIATQGPLSTTLLDFWRMIWEYSVLIIVMACMEYEMGKKKCERYWAEPGEMQLEFGPFSVSCEAEKRKSDYIIRTLKVKFNSETRTIYQFHYKNWPDHDVPSSIDPILELIWDVRCYQEDDSVPICIHCSAGCGRTGVICAIDYTWMLLKDGIIPENFSVFSLIREMRTQRPSLVQTQEQYELVYNAVLELFKRQMDVIRDKHSGTESQAKHCIPEKNHTLQADSYSPNLPKSTTKAAKMMNQQRTKMEIKESSSFDFRTSEISAKEELVLHPAKSSTSFDFLELNYSFDKNADTTMKWQTKAFPIVGEPLQKHQSLDLGSLLFEGCSNSKPVNAAGRYFNSKVPITRTKSTPFELIQQRETKEVDSKENFSYLESQPHDSCFVEMQAQKVMHVSSAELNYSLPYDSKHQIRNASNVKHHDSSALGVYSYIPLVENPYFSSWPPSGTSSKMSLDLPEKQDGTVFPSSLLPTSSTSLFSYYNSHDSLSLNSPTNISSLLNQESAVLATAPRIDDEIPPPLPVRTPESFIVVEEAGEFSPNVPKSLSSAVKVKIGTSLEWGGTSEPKKFDDSVILRPSKSVKLRSPKSELHQDRSSPPPPLPERTLESFFLADEDCMQAQSIETYSTSYPDTMENSTSSKQTLKTPGKSFTRSKSLKILRNMKKSICNSCPPNKPAESVQSNNSSSFLNFGFANRFSKPKGPRNPPPTWNI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Rabbit
100
Sheep
92
Dog
92
Chicken
92
Zebrafish
83
Pig
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y2R2 As Substrate

Site PTM Type Enzyme
K9 Ubiquitination
K17 Ubiquitination
K18 Ubiquitination
K21 Ubiquitination
K30 Ubiquitination
K32 Ubiquitination
S35 Phosphorylation Q05655 (PRKCD)
K51 Ubiquitination
K61 Ubiquitination
Y84 Phosphorylation
K166 Ubiquitination
T173 Phosphorylation
K300 Ubiquitination
K309 Ubiquitination
K315 Ubiquitination
S325 Phosphorylation
K330 Ubiquitination
K337 Ubiquitination
K364 Acetylation
K364 Ubiquitination
K373 Acetylation
K396 Ubiquitination
K400 Ubiquitination
K411 Ubiquitination
S414 Phosphorylation
K429 Ubiquitination
K441 Ubiquitination
T447 Phosphorylation
K448 Ubiquitination
S449 Phosphorylation
K461 Ubiquitination
K466 Ubiquitination
Y499 Phosphorylation
S500 Phosphorylation
K506 Ubiquitination
Y526 Phosphorylation
S527 Phosphorylation
Y528 Phosphorylation
Y536 Phosphorylation
S550 Phosphorylation
K640 Ubiquitination
K647 Ubiquitination
K649 Ubiquitination
S653 Phosphorylation
K663 Ubiquitination
K664 Ubiquitination
K675 Ubiquitination
S691 Phosphorylation
S692 Phosphorylation
T741 Phosphorylation
K744 Ubiquitination
T747 Phosphorylation
S751 Phosphorylation
K770 Ubiquitination

Research Backgrounds

Function:

Acts as negative regulator of T-cell receptor (TCR) signaling by direct dephosphorylation of the Src family kinases LCK and FYN, ITAMs of the TCRz/CD3 complex, as well as ZAP70, VAV, VCP and other key signaling molecules. Associates with and probably dephosphorylates CBL. Dephosphorylates LCK at its activating 'Tyr-394' residue. Dephosphorylates ZAP70 at its activating 'Tyr-493' residue. Dephosphorylates the immune system activator SKAP2. Positively regulates toll-like receptor (TLR)-induced type 1 interferon production. Promotes host antiviral responses mediated by type 1 interferon (By similarity). Regulates NOD2-induced pro-inflammatory cytokine secretion and autophagy.

PTMs:

Phosphorylation on Ser-35 by PKC/PRKCD abrogates its ability to dephosphorylate and inactivate the SRC family kinases.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in bone marrow, B and T-cells, PBMCs, natural killer cells, monocytes, dendritic cells and neutrophils. Both isoform 1 and 4 are predominantly expressed in lymphoid tissues and cells. Isoform 1 is expressed in thymocytes and both mature B and T-cells.

Subunit Structure:

Interacts with CSK. Interacts with LPXN (By similarity). Interacts with CBL. Interacts with TRAF3 (via MATH domain); the interaction promotes TRAF3 polyubiquitination.

Family&Domains:

Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily.

Restrictive clause

 

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