Histone H1.0 Antibody - #AF0359

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Product: Histone H1.0 Antibody
Catalog: AF0359
Description: Rabbit polyclonal antibody to Histone H1.0
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Xenopus
Mol.Wt.: 20kDa; 21kD(Calculated).
Uniprot: P07305
RRID: AB_2833524

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Bovine(91%), Sheep(91%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
Histone H1.0 Antibody detects endogenous levels of total Histone H1.0.
RRID:
AB_2833524
Cite Format: Affinity Biosciences Cat# AF0359, RRID:AB_2833524.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

H1 histone family member 0; H1(0); H10; H10_HUMAN; h1f0; H1FV; Histone H1''; Histone H1(0); Histone H1.0; Histone H10; Histone H5; MGC5241; N-terminally processed;

Immunogens

Immunogen:
Uniprot:

P07305(H10_HUMAN) >>Visit HPA database.

Gene(ID):
H1F0(3005)
Description:
H1F0 Histones H1 are necessary for the condensation of nucleosome chains into higher order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. Belongs to the histone H1/H5 family. Both the unedited and the RNA edited versions are induced by butyrate (at protein level). Only RNA edited version is induced by DTT, vinblastine or TNF (at protein level).
Sequence:
MTENSTSAPAAKPKRAKASKKSTDHPKYSDMIVAAIQAEKNRAGSSRQSIQKYIKSHYKVGENADSQIKLSIKRLVTTGVLKQTKGVGASGSFRLAKSDEPKKSVAFKKTKKEIKKVATPKKASKPKKAASKAPTKKPKATPVKKAKKKLAATPKKAKKPKTVKAKPVKASKPKKAKPVKPKAKSSAKRAGKKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Bovine
91
Sheep
91
Xenopus
83
Horse
0
Dog
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P07305 As Substrate

Site PTM Type Enzyme
M1 Acetylation
T2 Acetylation
T2 Phosphorylation
S5 Phosphorylation
S7 Phosphorylation
K12 Methylation
K14 Acetylation
K27 Acetylation
K27 Ubiquitination
K40 Ubiquitination
K52 Acetylation
K52 Ubiquitination
Y58 Phosphorylation
K59 Acetylation
K59 Methylation
K59 Ubiquitination
S66 Phosphorylation
K82 Methylation
K82 Ubiquitination
T84 Phosphorylation
K85 Ubiquitination
S90 Phosphorylation
S92 Phosphorylation
K102 Methylation
K108 Acetylation
K108 Methylation
T119 Phosphorylation
K121 Acetylation
S124 Phosphorylation
T141 Phosphorylation
K149 Ubiquitination
T153 Phosphorylation
K155 Methylation
K161 Acetylation
S171 Phosphorylation

Research Backgrounds

Function:

Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The histones H1.0 are found in cells that are in terminal stages of differentiation or that have low rates of cell division.

PTMs:

Phosphorylated on Ser-17 in RNA edited version.

ADP-ribosylated on Ser-104 in response to DNA damage.

Subcellular Location:

Nucleus. Chromosome.
Note: The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the histone H1/H5 family.

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