Product: Phospho-ERK8 (Thr175+Tyr177) Antibody
Catalog: AF0029
Source: Rabbit
Application: WB, IF/ICC, ELISA(peptide)
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 60kD; 60kD(Calculated).
Uniprot: Q8TD08
RRID: AB_2833512

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-ERK8 (Thr175+Tyr177) Antibody detects endogenous levels of ERK8 only when phosphorylated at Threonine 175 and Tyrosine 177.
RRID:
AB_2833512
Cite Format: Affinity Biosciences Cat# AF0029, RRID:AB_2833512.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ERK-7; ERK-8; ERK7; ERK8; Extracellular regulated kinase 8 delta; Extracellular signal regulated kinase 8; Extracellular signal-regulated kinase 7; Extracellular signal-regulated kinase 8; MAP kinase 15; MAPK 15; Mapk15; Mitogen activated protein kinase 15; Mitogen-activated protein kinase 15; MK15_HUMAN;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q8TD08 MK15_HUMAN:

Widely expressed with a maximal expression in lung and kidney.

Description:
ERK7 is phosphorylates MBP in vitro. Interacts with CSK/c-Src, ABL1 and RET. 3 isoforms of the human protein are produced by alternative splicing.
Sequence:
MCTVVDPRIVRRYLLRRQLGQGAYGIVWKAVDRRTGEVVAIKKIFDAFRDKTDAQRTFREITLLQEFGDHPNIISLLDVIRAENDRDIYLVFEFMDTDLNAVIRKGGLLQDVHVRSIFYQLLRATRFLHSGHVVHRDQKPSNVLLDANCTVKLCDFGLARSLGDLPEGPEDQAVTEYVATRWYRAPEVLLSSHRYTLGVDMWSLGCILGEMLRGRPLFPGTSTLHQLELILETIPPPSEEDLLALGSGCRASVLHQLGSRPRQTLDALLPPDTSPEALDLLRRLLVFAPDKRLSATQALQHPYVQRFHCPSDEWAREADVRPRAHEGVQLSVPEYRSRVYQMILECGGSSGTSREKGPEGVSPSQAHLHKPRADPQLPSRTPVQGPRPRPQSSPGHDPAEHESPRAAKNVPRQNSAPLLQTALLGNGERPPGAKEAPPLTLSLVKPSGRGAAPSLTSQAAAQVANQALIRGDWNRGGGVRVASVQQVPPRLPPEARPGRRMFSTSALQGAQGGARALLGGYSQAYGTVCHSALGHLPLLEGHHV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8TD08 As Substrate

Site PTM Type Enzyme
T175 Phosphorylation Q8TD08 (MAPK15)
Y177 Phosphorylation Q8TD08 (MAPK15)
S192 Phosphorylation Q8TD08 (MAPK15)
S331 Phosphorylation Q8TD08 (MAPK15)
Y340 Phosphorylation
T352 Phosphorylation Q8TD08 (MAPK15)
S362 Phosphorylation Q8TD08 (MAPK15)
S379 Phosphorylation Q8TD08 (MAPK15)
T381 Phosphorylation Q8TD08 (MAPK15)
S415 Phosphorylation
R449 Methylation

PTMs - Q8TD08 As Enzyme

Substrate Site Source
P05412 (JUN) S63 Uniprot
P05412 (JUN) S73 Uniprot
Q8TD08 (MAPK15) T175 Uniprot
Q8TD08 (MAPK15) Y177 Uniprot
Q8TD08 (MAPK15) S192 Uniprot
Q8TD08 (MAPK15) S331 Uniprot
Q8TD08 (MAPK15) T352 Uniprot
Q8TD08 (MAPK15) S362 Uniprot
Q8TD08 (MAPK15) S379 Uniprot
Q8TD08 (MAPK15) T381 Uniprot

Research Backgrounds

Function:

Atypical MAPK protein that regulates several process such as autophagy, ciliogenesis, protein trafficking/secretion and genome integrity, in a kinase activity-dependent manner. Controls both, basal and starvation-induced autophagy throught its interaction with GABARAP, MAP1LC3B and GABARAPL1 leading to autophagosome formation, SQSTM1 degradation and reduced MAP1LC3B inhibitory phosphorylation. Regulates primary cilium formation and the localization of ciliary proteins involved in cilium structure, transport, and signaling. Prevents the relocation of the sugar-adding enzymes from the Golgi to the endoplasmic reticulum, thereby restricting the production of sugar-coated proteins. Upon amino-acid starvation, mediates transitional endoplasmic reticulum site disassembly and inhibition of secretion. Binds to chromatin leading to MAPK15 activation and interaction with PCNA, that which protects genomic integrity by inhibiting MDM2-mediated degradation of PCNA. Regulates DA transporter (DAT) activity and protein expression via activation of RhoA. In response to H(2)O(2) treatment phosphorylates ELAVL1, thus preventing it from binding to the PDCD4 3'UTR and rendering the PDCD4 mRNA accessible to miR-21 and leading to its degradation and loss of protein expression. Also functions in a kinase activity-independent manner as a negative regulator of growth (By similarity). Phosphorylates in vitro FOS and MBP. During oocyte maturation, plays a key role in the microtubule organization and meiotic cell cycle progression in oocytes, fertilized eggs, and early embryos (By similarity). Interacts with ESRRA promoting its re-localization from the nucleus to the cytoplasm and then prevents its transcriptional activity.

PTMs:

Autophosphorylated on Thr-175 and Tyr-177; activates the enzyme.

Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase (By similarity).

Subcellular Location:

Cytoplasm>Cytoskeleton>Cilium basal body. Cell junction>Tight junction. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Cytoplasmic vesicle>Autophagosome. Golgi apparatus. Nucleus. Cytoplasm. Cytoplasm>Cytoskeleton>Spindle.
Note: Co-localizes to the cytoplasm only in presence of ESRRA (PubMed:21190936). Translocates to the nucleus upon activation (PubMed:20638370). At prometaphase I, metaphase I (MI), anaphase I, telophase I, and metaphase II (MII) stages, is stably detected at the spindle (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed with a maximal expression in lung and kidney.

Subunit Structure:

Interacts with CSK/c-Src, ABL1, RET and TGFB1I1. Interacts with GABARAP, MAP1LC3B and GABARAPL1; controls, in a kinase-dependent fashion, both basal and starvation-induced autophagy. Interacts with ESRRA; promotes re-localization of ESRRA to the cytoplasm through a XPO1-dependent mechanism then inhibits ESRRA transcriptional activity. Interacts with PCNA; the interaction is chromatin binding- and kinase activity-dependent and prevents MDM2-mediated PCNA destruction by inhibiting the association of PCNA with MDM2. Interacts with DVL2 (By similarity). Interacts with CLIC3; MAPK15 does not phosphorylates CLIC3 (By similarity).

Family&Domains:

The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteasomal degradation.

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Research Fields

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

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